Ethambutol (Synonyms: 乙胺丁醇)

目录号: HY-B0535 纯度: ≥98.0%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Ethambutol是抗分枝杆菌化合物，能通过抑制阿拉伯糖基转移酶活性阻止细胞壁的形成。

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Ethambutol Chemical Structure

CAS No. : 74-55-5

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥440	In-stock
5 mg	￥400	In-stock
10 mg	￥680	In-stock
25 mg	￥1360	In-stock
50 mg	￥2250	In-stock
100 mg	￥3800	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

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Customer Review

Other Forms of Ethambutol:

Ethambutol-d₄ In-stock
Ethambutol-d₁₀ In-stock
Ethambutol dihydrochloride In-stock
Ethambutol-d₈ 询价
Ethambutol (dihydrochloride) (Standard) 询价

MCE 顾客使用本产品发表的 1 篇科研文献

•ACS Chem Biol. 2021 Dec 15. [Abstract]

Ethambutol 相关产品

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生物活性
纯度 & 产品资料
参考文献

生物活性

Ethambutol is a bacteriostatic antimycobacterial agent, which obstructs the formation of cell wall by inhibiting arabinosyl transferases. Target: Antibacterial Ethambutol directly affects two polymers, arabinogalactan (AG) and lipoarabinomannan (LAM) in Mycobacterium smegmatis. In M. smegmatis, Ethambutol inhibits synthesis of arabinan completely and inhibits AG synthesis most likely as a consequence of this; more than 50% of the cell arabinan is released from the bacteria following Ethambutol treatment, whereas no galactan is released. Ethambutol main targets against embB gene product in M. avium. Ethambutol induces 60% changes in the embB gene in M. tuberculosis resistant mutants [1]. Ethambutol is effective against actively growing microorganisms of the genus Mycobacterium, including M. tuberculosis. Nearly all strains of M. tuberculosis and M. kansasii as well as a number of strains of the M. aviumcomplex (MAC) are sensitive to Ethambutol. [1] Ethambutol is potency against M. tuberculosis (H37Rv) with MIC of 0.5 μg/mL in vitro [2]. Ethambutol is efficient on treatment of mycobacterial-infected macrophages. When M. tuberculosis infected macrophages are treated with 6 μg/mL Ethambutol, the log CFUs following treatment for 3 days is 4.17, while value in control group is 4.8. The MICs for M. avium (MTCC 1723) and M. smegmatis (MTCC 6) are 15 μg/mL and 0.18 μg/mL, respectively. Ethambutol is efficient in animal model. 100 mg/kg Ethambutol given orally 15 days post i.v. infection 1 ×/week for 5 weeks, induces a lower log CFU compared with untreatment (4.59 vs 5.07) [3].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
BMDM	CC₅₀	100 μg/mL Compound: Ethambutol	Cytotoxicity against Swiss mouse BMDM assessed as reduction in cell viability after 24 hrs by MTS assay Cytotoxicity against Swiss mouse BMDM assessed as reduction in cell viability after 24 hrs by MTS assay	[PMID: 28712709]
HepG2	CC₅₀	> 400 μg/mL Compound: EMB	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33940463]
MRC5	CC₅₀	> 10 μg/mL Compound: EMB	Cytotoxicity against human MRC5 cells after 24 hrs Cytotoxicity against human MRC5 cells after 24 hrs	[PMID: 26254828]
RAW264.7	CC₅₀	≥ 400 μg/mL Compound: EMB	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33940463]
THP-1	EC₅₀	5.38 mg/L Compound: Ethambutol	Antimycobacterial activity against Mycobacterium avium ATCC 700898 infected in human THP1 cells assessed as extracellular bacterial killing after 7 days Antimycobacterial activity against Mycobacterium avium ATCC 700898 infected in human THP1 cells assessed as extracellular bacterial killing after 7 days	[PMID: 20231389]
THP-1	EC₅₀	7.93 mg/L Compound: Ethambutol	Antimycobacterial activity against Mycobacterium avium ATCC 700898 infected in human THP1 cells assessed as intracellular bacterial killing after 7 days Antimycobacterial activity against Mycobacterium avium ATCC 700898 infected in human THP1 cells assessed as intracellular bacterial killing after 7 days	[PMID: 20231389]
Vero	IC₅₀	> 150 μg/mL Compound: ethambutol	Cytotoxicity against Vero cells after 48 hrs by XTT method Cytotoxicity against Vero cells after 48 hrs by XTT method	[PMID: 17888665]
Vero	CC₅₀	> 188.5 μM Compound: Ethambutol	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as cell viability after 72 hr by MTT assay Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as cell viability after 72 hr by MTT assay	10.1007/s00044-011-9638-9
Vero	IC₅₀	> 200 μg/mL Compound: EMB	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30145051]
Vero	IC₅₀	> 305.9 μM Compound: Ethambutol	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19942326]
Vero	IC₅₀	> 305.9 μM Compound: ethambutol	Cytotoxicity against Vero cells after 72 hrs by MTT assay Cytotoxicity against Vero cells after 72 hrs by MTT assay	[PMID: 17276683]
Vero	IC₅₀	> 50 μg/mL Compound: Ethambutol	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24044875]
Vero	CC₅₀	> 500 μM Compound: EMB	Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay	[PMID: 22626551]
Vero	IC₅₀	> 6.25 μg/mL Compound: Ethambutol	Cytotoxicity against monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 28552337]
Vero	IC₅₀	> 62.5 μg/mL Compound: Ethambutol	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24721315]
Vero	IC₅₀	> 62.5 μg/mL Compound: Ethambutol	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 23352268]
Vero	CC₅₀	1470 μM Compound: EMB	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 21376586]
Vero	CC₅₀	38.51 μg/mL Compound: A, ETB	Cytotoxicity against african green monkey Vero cells after 72 hrs by resazurin assay Cytotoxicity against african green monkey Vero cells after 72 hrs by resazurin assay	[PMID: 23474387]
Vero	IC₅₀	390.93 μg/mL Compound: EMB	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by trypan blue staining based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by trypan blue staining based assay	[PMID: 28844400]

体内研究
(In Vivo)

诱导高尿酸血症^[5]

致病原理

尿酸水平升高可导致晶体沉积，并可能导致痛风、尿石症和尿酸盐肾病。Ethambutol 可通过降低肾脏尿酸清除率而导致尿酸增高，最后形成高尿酸血症^[4]^[5]。

具体造模方法

Rat^[4]: male • Wistar rats • SPF • 200-220 g
Administrtion: 250 mg/kg • intragastric administration • once daily for 23 days

Note

1. Ethambutol 诱导高尿酸模型宜与腺嘌呤 (HY-B0152) 联用 (100 mg/kg；灌胃)，模型构建效果更好^[4]。
2. 部分化合物可协同促进尿酸生成，如：Ethanol 和 Vitamin B12 (HY-B0315)^[5]。3. Ethambutol 诱导的高尿酸模型可被黄嘌呤氧化酶抑制剂控制。

造模成功指标

病理变化：胸腺和脾脏出现病理性损伤和炎症反应；胸腺萎缩、皮质髓质融合；脾窦充血，有铁血黄色沉积。
生理指标变化：血清尿酸水平↑；细胞稀疏、组织细胞数量↑、淋巴细胞数量↓。
分子层面变化：肾脏匀浆中 URAT1 水平↑，OAT1 水平↓。

拮抗产品 Xanthine Oxidase Inhibitors

诱导视神经病变

致病原理

Ethambutol 在一定剂量和时间范围内治疗可引起双眼中毒。目前尚无明确的治疗方法可以预防 Ethambutol 诱导视神经病变 (EON) 病例中视网膜神经元细胞的损伤。

具体造模方法

Rat^[6]: Wistar rats • Administration: 35 mg/kg/day &bull po for 30 days

Note

造模成功指标

分子变化：Bcl-2、Caspase-3 表达水平↑。
病理指标：神经节细胞损伤，视网膜神经节细胞 (RGC) 密度增加；由于神经节细胞胞浆中形成空泡，RGC 细胞层增厚。

拮抗产品 Citicoline (HY-B0739)

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

204.31

Formula

C₁₀H₂₄N₂O₂

CAS 号

74-55-5

性状

固体

颜色

White to off-white

中文名称

乙胺丁醇；乙二胺二丁醇；乙二胺丁醇

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

细胞实验：

DMSO 中的溶解度 : 100 mg/mL (489.45 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.8945 mL	24.4726 mL	48.9452 mL
5 mM	0.9789 mL	4.8945 mL	9.7890 mL
10 mM	0.4895 mL	2.4473 mL	4.8945 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: ≥98.0%

选择批次：

Data Sheet (634 KB) SDS (393 KB)

COA (280 KB) HNMR (140 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Ethambutol. Tuberculosis (Edinb), 2008. 88(2): p. 102-5. [Content Brief]

[2]. Rastogi, N., V. Labrousse, and K.S. Goh, In vitro activities of fourteen antimicrobial agents against drug susceptible and resistant clinical isolates of Mycobacterium tuberculosis and comparative intracellular activities against the virulent H37Rv strain in human macrophages. Curr Microbiol, 1996. 33(3): p. 167-75. [Content Brief]

[3]. Kaur, D. and G.K. Khuller, In vitro, ex-vivo and in vivo activities of ethambutol and sparfloxacin alone and in combination against mycobacteria. Int J Antimicrob Agents, 2001. 17(1): p. 51-5. [Content Brief]

[4]. Kang L, Miao JX, Cao LH, Miao YY, Miao MS, Liu HJ, Xiang LL, Song YG. Total glucosides of herbaceous peony (Paeonia lactiflora Pall.) flower attenuate adenine- and ethambutol-induced hyperuricaemia in rats. J Ethnopharmacol. 2020 Oct 28;261:113054. [Content Brief]

[5]. Pham AQ, Doan A, Andersen M. Pyrazinamide-induced hyperuricemia. P T. 2014 Oct;39(10):695-715. [Content Brief]

[6]. Nusanti S, Sari RI, Siregar NC, Sidik M. The Effect of Citicoline on Ethambutol Optic Neuropathy: Histopathology and Immunohistochemistry Analysis of Retina Ganglion Cell Damage Level in Rat Model. J Ocul Pharmacol Ther. 2022 Oct;38(8):584-589. [Content Brief]

Ethambutol 相关分类

Infection

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.8945 mL	24.4726 mL	48.9452 mL	122.3631 mL
	5 mM	0.9789 mL	4.8945 mL	9.7890 mL	24.4726 mL
	10 mM	0.4895 mL	2.4473 mL	4.8945 mL	12.2363 mL
	15 mM	0.3263 mL	1.6315 mL	3.2630 mL	8.1575 mL
	20 mM	0.2447 mL	1.2236 mL	2.4473 mL	6.1182 mL
	25 mM	0.1958 mL	0.9789 mL	1.9578 mL	4.8945 mL
	30 mM	0.1632 mL	0.8158 mL	1.6315 mL	4.0788 mL
	40 mM	0.1224 mL	0.6118 mL	1.2236 mL	3.0591 mL
	50 mM	0.0979 mL	0.4895 mL	0.9789 mL	2.4473 mL
	60 mM	0.0816 mL	0.4079 mL	0.8158 mL	2.0394 mL
	80 mM	0.0612 mL	0.3059 mL	0.6118 mL	1.5295 mL
	100 mM	0.0489 mL	0.2447 mL	0.4895 mL	1.2236 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethambutol74-55-5乙胺丁醇乙二胺二丁醇乙二胺丁醇BacterialAntibioticInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Ethambutol (Synonyms: 乙胺丁醇)

Customer Review

Other Forms of Ethambutol:

MCE 顾客使用本产品发表的 1 篇科研文献