Apolipoprotein A-I/APOA1 蛋白, Human (HEK293, His)

Apolipoprotein A-I Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein A-I (ApoA-I), the major protein component in high-density lipoprotein (HDL), plays key roles in the Reverse Cholesterol Transport pathway. Apolipoprotein A-I is associated with dengue virus (DV) particles and enhances virus infection through SR-BI^[1][2][3].

载脂蛋白 A1 (ApoA1) 是一种 28 kDa 糖蛋白，是高密度脂蛋白 (HDL) 颗粒的主要蛋白质成分。HDL 颗粒在胆固醇从外周组织反向转运至肝脏的过程中发挥着核心作用。在过去的十年中，重组HDL（rHDL）已被用作治疗动脉粥样硬化的治疗剂。rHDL 促进胆固醇从外周细胞流出的能力已被证明可以减少动脉粥样硬化斑块病变的大小^[2]。

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Apolipoprotein A-I/ApoA1 at 5 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human SR-AI/MSR. The ED₅₀ for this effect is <1.2 µg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Apolipoprotein A-I/ApoA1 at 5 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human SR-AI/MSR.The ED₅₀ for this effect is 1.196 µg/mL.

Human

HEK293

C-6*His

P02647 (R19-Q267)

335  [NCBI]

RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQHHHHHH

Approximately 32.22 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ikon N, et al. A facile method for isolation of recombinant human apolipoprotein A-I from E. coli. Protein Expr Purif. 2017 Jun;134:18-24.  [Content Brief]

  [2]. Obici L, et al. Structure, function and amyloidogenic propensity of apolipoprotein A-I. Amyloid. 2006 Dec;13(4):191-205.  [Content Brief]

  [3]. Li Y, et al. Human apolipoprotein A-I is associated with dengue virus and enhances virus infection through SR-BI. PLoS One. 2013 Jul 24;8(7):e70390.  [Content Brief]