1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, Influenza B (EPI529345, sf9, His)

HA/Hemagglutinin 蛋白, Influenza B (EPI529345, sf9, His)

目录号: HY-P73935
产品使用指南

HA/Hemagglutinin 蛋白是 I 类融合蛋白,作为附着因子和膜融合蛋白具有多种功能。HA/Hemagglutinin 蛋白 可与含有唾液酸的细胞表面受体结合,促进病毒颗粒附着在宿主细胞上,对病毒宿主范围限制和毒力有重要作用。HA/Hemagglutinin 蛋白, Influenza B (EPI529345, sf9, His) 是重组的 HA/血凝素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。HA/Hemagglutinin 蛋白, Influenza B (EPI529345, sf9, His) 全长 547 个氨基酸,分子量约为 ~59.1 kDa。

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描述

HA/Hemagglutinin proteins are Class I fusion proteins with multiple functions as attachment factors and membrane fusion proteins. HA/Hemagglutinin proteins can bind to cell surface receptors containing sialic acid, promote the attachment of virus particles to host cells, and play an important role in viral host range limitation and virulence. HA/Hemagglutinin Protein, Influenza B (EPI529345, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HA/Hemagglutinin Protein, Influenza B (EPI529345, sf9, His) is 547 a.a., with molecular weight of ~59.1 kDa.

研究背景

血凝素是 I 类融合蛋白,作为附着因子和膜融合蛋白具有多种功能,在病毒生命周期中起着至关重要的作用。它与含有唾液酸的细胞表面受体结合,促进病毒颗粒附着在宿主细胞上。这种附着通过依赖网格蛋白的内吞途径或不依赖网格蛋白和小洞蛋白的途径导致病毒粒子的内化。透明质酸蛋白是一类病毒融合蛋白,负责介导内吞病毒颗粒膜与内体膜的融合。这种融合事件允许病毒进入细胞的细胞质。核内体的低 pH 环境触发蛋白质 HA2 亚基的不可逆构象变化,导致融合疏水肽的释放。多个三聚体的 HA 蛋白是必要的,以形成一个合格的融合孔。透明质酸蛋白在受体结合、病毒内化、融合和膜穿透中的功能是决定病毒宿主范围限制和毒力的关键。流感血凝素 (HA) 是在流感病毒表面发现的一种同源三聚体糖蛋白,是其传染性的组成部分。

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

EPI529345 (M1-T547)

基因 ID

/

蛋白结构
N-term
HA (M1-T547)
Accession # EPI529345
His
C-term
同用名
HA; Hemagglutinin; HA/Hemagglutinin, Influenza B (B/PHUKET/3073/2013, sf9, His)
分子量

Approximately 59.1 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA/Hemagglutinin Protein, Influenza B (EPI529345, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA/Hemagglutinin Protein, Influenza B (EPI529345, sf9, His)
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HY-P73935
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