Siglec-2/CD22 蛋白, Mouse (690a.a, HEK293, Fc)

Siglec-2/CD22 Protein, Mouse (690a.a, HEK293, Fc) is a Siglec of ~140 kDa with seven Ig-like domains and is expressed in a B-cell lineage-specific fashion, starting at the pre-B cell stage. CD22 is associated with membrane-bound IgM on the surface of B cells. Siglec-2 (CD22) specifically recognizes 伪2.6 sialic acid-containing glycans. Siglec-2 negatively regulates B cell antigen receptor (BCR) signaling by recruitment and activation of SH2-containing phosphatase 1 (SHP-1), which counteracts the phospharylation-mediated activation of signaling molecules by dephosphorylation, and reduces Ca²⁺ signaling by regulating the activity of the Ca²⁺ pump PMCA^[1][2]. Siglec-2/CD22 Protein, Mouse (690a.a, HEK293, Fc) is the recombinant mouse-derived Siglec-2/CD22 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-2/CD22 Protein, Mouse (690a.a, HEK293, Fc) is 690 a.a., with molecular weight of ~125 kDa.

Siglec-2 (CD22) 特异性识别含 α2.6 唾液酸的聚糖，并在 B 细胞、抗体分泌细胞的前体、浆细胞上表达。Siglec-2 通过 B 细胞抗原受体 (BCR) 负向调节信号传导，BCR 在 B 细胞对抗原刺激的反应中发挥着至关重要的作用。Siglec-2 通过招募和激活含 SH2 的磷酸酶 1 (SHP-1) 负向调节 BCR 信号传导，从而通过去磷酸化抵消磷酸化介导的信号分子激活，并通过调节 Ca2+ 泵 PMCA 的活性来减少 Ca2+ 信号传导。抗原刺激 BCR 后，CD22 在其胞内尾部被酪氨酸磷酸化。CD22 表现出网格蛋白介导的内吞作用和运输至回收室的特征^[1][2]。

Mouse

HEK293

C-hFc

BAE33829 (R19-R708)

12483  [NCBI]

Approximately 125 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.