Amonafide (Synonyms: 氨萘非特; AS1413)

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Amonafide 是一种拓扑异构酶 II (topoisomerase II) 的抑制剂和 DNA 的嵌入剂，通过抑制 Topo II 与 DNA 的结合，来诱导凋亡信号通路的发生。

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Amonafide Chemical Structure

CAS No. : 69408-81-7

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥737	In-stock
1 mg	￥268	In-stock
5 mg	￥669	In-stock
10 mg	￥837	In-stock
25 mg	￥1758	In-stock
50 mg	￥2790	In-stock
100 mg	￥4669	In-stock
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Customer Review

Other Forms of Amonafide:

Amonafide dihydrochloride 询价

MCE 顾客使用本产品发表的 3 篇科研文献

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Topoisomerase Topo I Topo II

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Amonafide is a topoisomerase II inhibitor and DNA intercalator that induces apoptotic signaling by blocking the binding of Topo II to DNA.

IC₅₀ & Target^[1]

Topoisomerase II

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A 172	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human A172 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human A172 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
A 172	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human A172 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human A172 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
A 172	IC₅₀	23.81 μM Compound: Amonafide	Cytotoxicity against human A 172 cells assessed as cell viability measured after 96 hrs by XTT assay Cytotoxicity against human A 172 cells assessed as cell viability measured after 96 hrs by XTT assay	[PMID: 34507011]
A2780	IC₅₀	11.66 μM Compound: Amonafide	Antiproliferative activity against human A2780 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human A2780 cells assessed as cell growth inhibition by MTT assay	[PMID: 36894107]
A-375	IC₅₀	14.7 μM Compound: B2	Cytotoxicity against human A375 cells in hypoxic condition after 24 hrs by MTT assay Cytotoxicity against human A375 cells in hypoxic condition after 24 hrs by MTT assay	[PMID: 17331719]
A-375	IC₅₀	7.51 μM Compound: Amonafide	Cytotoxicity against human A375 cells by MTT assay Cytotoxicity against human A375 cells by MTT assay	[PMID: 19643513]
A549	IC₅₀	1.1 μM Compound: Am	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 23353750]
A549	IC₅₀	1.1 μM Compound: B2	Cytotoxicity against human A549 cells by sulforhodamine B dye staining method Cytotoxicity against human A549 cells by sulforhodamine B dye staining method	[PMID: 17331719]
A549	IC₅₀	1.59 μM Compound: Amonafide	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	10.1039/C5MD00543D
A549	IC₅₀	1100 nM Compound: amonafide	Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B dye assay Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B dye assay	[PMID: 17707644]
A549	IC₅₀	1100 nM Compound: 1	Tested for inhibitory activity against human tumor cell line A549 (a non small, drug resistant cell line that does not produce P-glycoprotein) of lung carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line A549 (a non small, drug resistant cell line that does not produce P-glycoprotein) of lung carcinoma using sulforhodamine B assay.	[PMID: 10956214]
A549	IC₅₀	1100 nM Compound: Amonafide	Cytotoxic concentration against human A549 lung cancer cell line determined by sulforhodamine B dye-staining method Cytotoxic concentration against human A549 lung cancer cell line determined by sulforhodamine B dye-staining method	[PMID: 15876532]
A549	IC₅₀	13 μM Compound: Amonafide	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 24370011]
A549	IC₅₀	13 μM Compound: Amonafide	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 19643513]
A549	IC₅₀	13 μM Compound: amonafide	Growth inhibition of human A549 cells by SRB assay Growth inhibition of human A549 cells by SRB assay	[PMID: 17532640]
A549	IC₅₀	2 μM Compound: Amonafide	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 29342415]
A549	IC₅₀	23.46 μM Compound: Amonafide	Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 33965862]
A549	IC₅₀	3.2 μM Compound: Am	Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay	[PMID: 23770449]
A549	IC₅₀	3.8 μM Compound: 1, amonafide	Antiproliferative activity against A549 cells after 72 hrs by MTT assay Antiproliferative activity against A549 cells after 72 hrs by MTT assay	[PMID: 17658777]
A549	IC₅₀	7.76 μM Compound: 1	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30774856]
A549	IC₅₀	7.94 μM Compound: Amonafide	Antiproliferative activity against human A549 cells by MTT assay Antiproliferative activity against human A549 cells by MTT assay	[PMID: 33109400]
A549	IC₅₀	8.1 μM Compound: Amonafide	Growth inhibition of human A549 cells measured after 72 hrs by SRB assay Growth inhibition of human A549 cells measured after 72 hrs by SRB assay	[PMID: 22000922]
A549	IC₅₀	9.56 μM Compound: Amonafide	Cytotoxicity against cisplatin-resistant human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against cisplatin-resistant human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 33965862]
B16-F10	IC₅₀	6.7 μM Compound: Amonafide	Cytotoxicity against mouse B16F10 cells measured after 2 days by MTS assay Cytotoxicity against mouse B16F10 cells measured after 2 days by MTS assay	[PMID: 27939348]
BT-474	IC₅₀	37.8 μM Compound: Amonafide	Cytotoxicity against human BT474 cells after 3 days by MTT assay Cytotoxicity against human BT474 cells after 3 days by MTT assay	[PMID: 24835201]
Cancer cell lines	IC₅₀	2031 nM Compound: 2	Tested against UACC375 human melanoma cancer cells by sulforhodamine B (SRB) assay Tested against UACC375 human melanoma cancer cells by sulforhodamine B (SRB) assay	[PMID: 8459403]
Ca-Ski	IC₅₀	9.96 μM Compound: Amonafide	Cytotoxicity against human CaSki cells after 3 days by MTT assay Cytotoxicity against human CaSki cells after 3 days by MTT assay	[PMID: 24835201]
CNE-2	IC₅₀	8.65 μM Compound: Amonafide	Antiproliferative activity against human CNE-2 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human CNE-2 cells assessed as cell growth inhibition by MTT assay	[PMID: 36894107]
CT26	IC₅₀	11.69 μM Compound: Amonafide	Growth inhibition of mouse CT26 cells after 24 hrs by MTT assay Growth inhibition of mouse CT26 cells after 24 hrs by MTT assay	[PMID: 29990424]
GES1	IC₅₀	21.6 μM Compound: Am	Cytotoxicity against human GES-1 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human GES-1 cells after 72 hrs by sulforhodamine B assay	[PMID: 23770449]
HCT-116	IC₅₀	15.3 μM Compound: Amonafide	Growth inhibition of human HCT116 cells measured after 72 hrs by SRB assay Growth inhibition of human HCT116 cells measured after 72 hrs by SRB assay	[PMID: 22000922]
HCT-116	IC₅₀	2.7 μM Compound: Amonafide	Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth incubated for 24 hrs by XTT assay Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth incubated for 24 hrs by XTT assay	[PMID: 30312931]
HCT-116	IC₅₀	32 μM Compound: amonafide	Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 22428910]
HCT-116	IC₅₀	4.55 μM Compound: 3, ST	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 22138307]
HCT-116	IC₅₀	6.46 μM Compound: Amonafide	Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 30151093]
HCT-116	IC₅₀	6.86 μM Compound: Amonafide	Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 29133040]
HCT-116	IC₅₀	7.18 μM Compound: Amonafide	Growth inhibition of human HCT116 cells after 48 hrs by MTT assay Growth inhibition of human HCT116 cells after 48 hrs by MTT assay	[PMID: 29990424]
HCT-116	IC₅₀	8.55 μM Compound: Amonafide	Growth inhibition of human HCT116 cells after 24 hrs by MTT assay Growth inhibition of human HCT116 cells after 24 hrs by MTT assay	[PMID: 29990424]
HCT-15	IC₅₀	5.2 μM Compound: 1, amonafide	Antiproliferative activity against HCT15 cells after 72 hrs by MTT assay Antiproliferative activity against HCT15 cells after 72 hrs by MTT assay	[PMID: 17658777]
HCT-8	IC₅₀	9.26 μM Compound: Amonafide	Cytotoxicity against human HCT8 cells by MTT assay Cytotoxicity against human HCT8 cells by MTT assay	[PMID: 19643513]
HEL	IC₅₀	3.4 μM Compound: AM	Antiproliferative activity against human HEL cells after 48 hrs by MTT assay Antiproliferative activity against human HEL cells after 48 hrs by MTT assay	[PMID: 24054489]
HEL	IC₅₀	4 μM Compound: Amonafide	Antiproliferative activity against human HEL cells after 48 hrs by MTT assay Antiproliferative activity against human HEL cells after 48 hrs by MTT assay	[PMID: 28233678]
HeLa	IC₅₀	0.68 μM Compound: amonafide	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 19070496]
HeLa	IC₅₀	1.4 μM Compound: Amonafide	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19643513]
HeLa	IC₅₀	1.4 μM Compound: amonafide	Growth inhibition of human HeLa cells by MTT assay Growth inhibition of human HeLa cells by MTT assay	[PMID: 17532640]
HeLa	IC₅₀	1.73 μM Compound: Amonafide	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 21345546]
HeLa	IC₅₀	10.67 μM Compound: Amonofide	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 20170164]
HeLa	IC₅₀	3.41 μM Compound: Amonafide	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 29342415]
HeLa	IC₅₀	4.36 μM Compound: Amonafide	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	10.1039/C5MD00543D
HeLa	IC₅₀	6.02 μM Compound: amonafide	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 21208805]
HeLa	IC₅₀	6.71 μM Compound: Amonafide	Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 24370011]
HeLa	IC₅₀	9.52 μM Compound: AM	Cytotoxicity against human HeLa cells incubated for 24 hrs by sulforhodamine B assay Cytotoxicity against human HeLa cells incubated for 24 hrs by sulforhodamine B assay	[PMID: 26211460]
HepG2	IC₅₀	1.41 μM Compound: 3, ST	Cytotoxicity against human HepG2 after 72 hrs by MTT assay Cytotoxicity against human HepG2 after 72 hrs by MTT assay	[PMID: 22138307]
HepG2	IC₅₀	1.71 μM Compound: Amonafide	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 24835201]
HepG2	IC₅₀	10.11 μM Compound: Amonafide	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 28177238]
HepG2	IC₅₀	11.67 μM Compound: Amonafide	Growth inhibition of human HepG2 cells after 48 hrs by MTT assay Growth inhibition of human HepG2 cells after 48 hrs by MTT assay	[PMID: 29990424]
HepG2	IC₅₀	11.67 μM Compound: Amonafide	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 29133040]
HepG2	IC₅₀	36 μM Compound: amonafide	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 22428910]
HepG2	IC₅₀	6.45 μM Compound: amonafide	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 21208805]
HepG2	IC₅₀	9.38 μM Compound: Amonafide	Cytotoxicity against human HepG2 cells assessed as inhibitory of cell growth measured after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as inhibitory of cell growth measured after 24 hrs by MTT assay	[PMID: 33965862]
HepG2	IC₅₀	9.38 μM Compound: Amonafide	Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 33965862]
HepG2	IC₅₀	9.45 μM Compound: Amonafide	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 30151093]
HL-60	IC₅₀	0.9 μM Compound: Am	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 23770449]
HL-60	IC₅₀	1.63 μM Compound: amonafide	Growth inhibition of human HL60 cells by MTT assay Growth inhibition of human HL60 cells by MTT assay	[PMID: 17532640]
HL-60	IC₅₀	17.96 μM Compound: Amonofide	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 20170164]
HL-60	IC₅₀	17.96 μM Compound: Amonafide	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 19643513]
HL-60	IC₅₀	3.4 μM Compound: Amonafide	Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay	[PMID: 28233678]
Hs 683	IC₅₀	3.9 μM Compound: 1, amonafide	Antiproliferative activity against Hs683 cells after 72 hrs by MTT assay Antiproliferative activity against Hs683 cells after 72 hrs by MTT assay	[PMID: 17658777]
HT-29	IC₅₀	5.46 μM Compound: 1	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30774856]
Huh-7	IC₅₀	8.52 μM Compound: Amonafide	Cytotoxicity against human Huh-7 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay Cytotoxicity against human Huh-7 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay	[PMID: 33965862]
Huh-7	IC₅₀	8.52 μM Compound: Amonafide	Cytotoxicity against human Huh-7 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against human Huh-7 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 33965862]
K562	IC₅₀	> 50 μM Compound: Amonafide	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 22436386]
K562	IC₅₀	10.1 μM Compound: Amonafide	Antiproliferative activity against human K562 cells after 48 hrs by MTT assay Antiproliferative activity against human K562 cells after 48 hrs by MTT assay	[PMID: 29133040]
K562	IC₅₀	20.11 μM Compound: Amonafide	Growth inhibition of human K562 cells after 48 hrs by MTT assay Growth inhibition of human K562 cells after 48 hrs by MTT assay	[PMID: 29990424]
K562	IC₅₀	31.7 μM Compound: amonafide	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 22428910]
L02	IC₅₀	11.56 μM Compound: Amonafide	Cytotoxicity against human HL7702 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay Cytotoxicity against human HL7702 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay	[PMID: 33965862]
L02	IC₅₀	13.8 μM Compound: Am	Cytotoxicity against human LO2 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human LO2 cells after 72 hrs by sulforhodamine B assay	[PMID: 23770449]
L02	IC₅₀	5 μM Compound: Am	Cytotoxicity against human LO2 cells by SRB assay Cytotoxicity against human LO2 cells by SRB assay	[PMID: 23353750]
L1210	IC₅₀	625 nM Compound: 1	Antitumor activity against murine L1210 resistant cell line by using MTT assay Antitumor activity against murine L1210 resistant cell line by using MTT assay	[PMID: 7699715]
L1210	IC₅₀	625 nM Compound: 1	Anti-tumor activity against murine L1210 sensitive cell line by using MTT assay Anti-tumor activity against murine L1210 sensitive cell line by using MTT assay	[PMID: 7699715]
L1210	IC₅₀	625 nM Compound: 1	Tested for inhibitory activity against murine cell line L12110, a strain resistant to doxorubicin using MTT assay. Tested for inhibitory activity against murine cell line L12110, a strain resistant to doxorubicin using MTT assay.	[PMID: 10956214]
L1210	IC₅₀	625 nM Compound: 1	Tested for inhibitory activity against murine cell line L12110, a strain sensitive to doxorubicin using MTT assay. Tested for inhibitory activity against murine cell line L12110, a strain sensitive to doxorubicin using MTT assay.	[PMID: 10956214]
L1210	IC₅₀	625 nM Compound: 2	Tested against L1210 resistant murine leukemia cells by MTT tetrazolium dye assay Tested against L1210 resistant murine leukemia cells by MTT tetrazolium dye assay	[PMID: 8459403]
L1210	IC₅₀	625 nM Compound: 2	Tested against L1210 sensitive murine leukemia cells by MTT tetrazolium dye assay Tested against L1210 sensitive murine leukemia cells by MTT tetrazolium dye assay	[PMID: 8459403]
LoVo	IC₅₀	2.7 μM Compound: 1, amonafide	Antiproliferative activity against LoVo cells after 72 hrs by MTT assay Antiproliferative activity against LoVo cells after 72 hrs by MTT assay	[PMID: 17658777]
LS174T	IC₅₀	20.28 μM Compound: Amonofide	Cytotoxicity against human LS 174T cells after 72 hrs by MTT assay Cytotoxicity against human LS 174T cells after 72 hrs by MTT assay	[PMID: 20170164]
MCF7	IC₅₀	1.68 μM Compound: Amonafide	Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 24370011]
MCF7	IC₅₀	1.68 μM Compound: Amonafide	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 21345546]
MCF7	IC₅₀	10.93 μM Compound: Amonafide	Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 33965862]
MCF7	IC₅₀	11.88 μM Compound: Amonofide	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 20170164]
MCF7	IC₅₀	11.88 μM Compound: Amonafide	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 19643513]
MCF7	IC₅₀	1100 nM Compound: 1	Tested for inhibitory activity against human tumor cell line MCF7, a strain sensitive to doxorubicin of breast carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line MCF7, a strain sensitive to doxorubicin of breast carcinoma using sulforhodamine B assay.	[PMID: 10956214]
MCF7	IC₅₀	12.92 μM Compound: Amonafide	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 30151093]
MCF7	IC₅₀	1200 nM Compound: 1	Tested for inhibitory activity against human tumor cell line MCF7, a strain resistant to mitoxantrone of breast carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line MCF7, a strain resistant to mitoxantrone of breast carcinoma using sulforhodamine B assay.	[PMID: 10956214]
MCF7	IC₅₀	1800 nM Compound: 1	Tested for inhibitory activity against human tumor cell line D40, a strain resistant to doxorubicin of breast carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line D40, a strain resistant to doxorubicin of breast carcinoma using sulforhodamine B assay.	[PMID: 10956214]
MCF7	IC₅₀	4.05 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30774856]
MCF7	IC₅₀	5.8 μM Compound: 1, amonafide	Antiproliferative activity against MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against MCF7 cells after 72 hrs by MTT assay	[PMID: 17658777]
MCF7	IC₅₀	6.79 μM Compound: Amonafide	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 29342415]
MCF7	IC₅₀	8.02 μM Compound: Amonafide	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	10.1039/C5MD00543D
MDA-MB-231	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
MDA-MB-231	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human MDA-MB-231 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
MDA-MB-231	IC₅₀	15.23 μM Compound: Amonafide	Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 33965862]
MDA-MB-231	IC₅₀	4.4 μM Compound: Am	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by sulforhodamine B assay	[PMID: 23770449]
MDA-MB-231	IC₅₀	42 μM Compound: amonafide	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 22428910]
MDA-MB-231	IC₅₀	6.62 μM Compound: Amonafide	Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 30151093]
MDA-MB-238	IC₅₀	24.25 μM Compound: Amonafide	Cytotoxicity against human MDA-MB-238 cells assessed as cell viability measured after 96 hrs by XTT assay Cytotoxicity against human MDA-MB-238 cells assessed as cell viability measured after 96 hrs by XTT assay	[PMID: 34507011]
MGC-803	IC₅₀	3.23 μM Compound: Amonafide	Cytotoxicity against human MGC803 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MGC803 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 29342415]
MGC-803	IC₅₀	5.25 μM Compound: Amonafide	Antiproliferative activity against human MGC-803 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human MGC-803 cells assessed as cell growth inhibition by MTT assay	[PMID: 36894107]
MGC-803	IC₅₀	9.06 μM Compound: Amonafide	Antiproliferative activity against human MGC-803 cells by MTT assay Antiproliferative activity against human MGC-803 cells by MTT assay	[PMID: 33109400]
MKN-45	IC₅₀	> 50 μM Compound: Amonofide	Cytotoxicity against human MKN45 cells after 72 hrs by MTT assay Cytotoxicity against human MKN45 cells after 72 hrs by MTT assay	[PMID: 20170164]
NCI-H1299	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
NCI-H1299	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human NCI-H1299 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
NCI-H1299	IC₅₀	8.84 μM Compound: Amonafide	Cytotoxicity against human NCI-H1299 cells assessed as cell viability measured after 96 hrs by XTT assay Cytotoxicity against human NCI-H1299 cells assessed as cell viability measured after 96 hrs by XTT assay	[PMID: 34507011]
NCI-H460	IC₅₀	7.8 μM Compound: amonafide	Antiproliferative activity against human NCI-H460 cells after 72 hrs Antiproliferative activity against human NCI-H460 cells after 72 hrs	[PMID: 18061459]
OVCAR-3	IC₅₀	2180 nM Compound: 1	Anti-tumor activity against human OVCAR-3 ovarian cancer cell lines by using MTT assay Anti-tumor activity against human OVCAR-3 ovarian cancer cell lines by using MTT assay	[PMID: 7699715]
OVCAR-3	IC₅₀	2180 nM Compound: 2	Tested against OVCAR-3 human ovarian cancer cells by sulforhodamine B (SRB) assay Tested against OVCAR-3 human ovarian cancer cells by sulforhodamine B (SRB) assay	[PMID: 8459403]
OVCAR-3	IC₅₀	2180 nM Compound: 1	Tested for inhibitory activity against human tumor cell line OVCAR-3, a resistant ovarian carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line OVCAR-3, a resistant ovarian carcinoma using sulforhodamine B assay.	[PMID: 10956214]
P388	IC₅₀	0.2 μM Compound: Am	Cytotoxicity against mouse P388 cells by tetrazolium-formazan assay Cytotoxicity against mouse P388 cells by tetrazolium-formazan assay	[PMID: 23353750]
P388	IC₅₀	0.2 μM Compound: B2	Cytotoxicity against mouse P388 cells by tetrazolium-formazan method Cytotoxicity against mouse P388 cells by tetrazolium-formazan method	[PMID: 17331719]
P388	IC₅₀	200 nM Compound: amonafide	Cytotoxicity against mouse P388 cells after 48 hrs by tetrazolium-formazan method Cytotoxicity against mouse P388 cells after 48 hrs by tetrazolium-formazan method	[PMID: 17707644]
P388	IC₅₀	200 nM Compound: Amonafide	Cytotoxic concentration against murine P388 leukemia cell line growth determined by microculture tetrazolium-formazan method Cytotoxic concentration against murine P388 leukemia cell line growth determined by microculture tetrazolium-formazan method	[PMID: 15876532]
P388	IC₅₀	4.56 μM Compound: Amonafide	Cytotoxicity against mouse P388 cells by MTT assay Cytotoxicity against mouse P388 cells by MTT assay	[PMID: 19643513]
P388	IC₅₀	4.56 μM Compound: amonafide	Growth inhibition of mouse P388 cells by MTT assay Growth inhibition of mouse P388 cells by MTT assay	[PMID: 17532640]
P388D1	IC₅₀	0.68 μM Compound: amonafide	Antiproliferative activity against mouse P388D1 cells after 48 hrs by MTT assay Antiproliferative activity against mouse P388D1 cells after 48 hrs by MTT assay	[PMID: 21208805]
P388D1	IC₅₀	6.02 μM Compound: amonafide	Cytotoxicity against mouse P388D1 cells after 48 hrs by MTT assay Cytotoxicity against mouse P388D1 cells after 48 hrs by MTT assay	[PMID: 19070496]
PANC-1	IC₅₀	2.7 μM Compound: Amonafide	Cytotoxicity against human PANC-1 cells assessed as inhibition of cell growth incubated for 24 hrs by XTT assay Cytotoxicity against human PANC-1 cells assessed as inhibition of cell growth incubated for 24 hrs by XTT assay	[PMID: 30312931]
PC-3	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human PC3 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human PC3 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
PC-3	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human PC3 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human PC3 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
PC-3	IC₅₀	8.05 μM Compound: 3, ST	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 22138307]
SGC-7901	IC₅₀	5.32 μM Compound: Amonafide	Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay	10.1039/C5MD00543D
SK-BR-3	IC₅₀	7.4 μM Compound: Amonafide	Antiproliferative activity against human SK-BR-3 cells after 48 hrs by MTT assay Antiproliferative activity against human SK-BR-3 cells after 48 hrs by MTT assay	[PMID: 28233678]
SK-BR-3	IC₅₀	7.4 μM Compound: AM	Antiproliferative activity against human SKBR3 cells after 48 hrs by MTT assay Antiproliferative activity against human SKBR3 cells after 48 hrs by MTT assay	[PMID: 24054489]
SK-OV-3	IC₅₀	6.31 μM Compound: Amonafide	Antiproliferative activity against human SK-OV-3 cells by MTT assay Antiproliferative activity against human SK-OV-3 cells by MTT assay	[PMID: 33109400]
SK-OV-3	IC₅₀	8.83 μM Compound: 3, ST	Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay	[PMID: 22138307]
SMMC-7721	IC₅₀	10.32 μM Compound: Amonafide	Growth inhibition of human SMMC7721 cells after 48 hrs by MTT assay Growth inhibition of human SMMC7721 cells after 48 hrs by MTT assay	[PMID: 29990424]
SMMC-7721	IC₅₀	10.32 μM Compound: Amonafide	Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay	[PMID: 29133040]
SMMC-7721	IC₅₀	4.15 μM Compound: amonafide	Growth inhibition of human SMMC7721 cells by MTT assay Growth inhibition of human SMMC7721 cells by MTT assay	[PMID: 17532640]
SMMC-7721	IC₅₀	4.27 μM Compound: Amonafide	Cytotoxicity against human SMMC7721 cells after 48 hrs by MTT assay Cytotoxicity against human SMMC7721 cells after 48 hrs by MTT assay	[PMID: 21345546]
SMMC-7721	IC₅₀	6.93 μM Compound: Amonafide	Antiproliferative activity against human SMMC-7721 cells by MTT assay Antiproliferative activity against human SMMC-7721 cells by MTT assay	[PMID: 33109400]
SMMC-7721	IC₅₀	9.37 μM Compound: Amonafide	Cytotoxicity against human SMMC7721 cells after 48 hrs by MTT assay Cytotoxicity against human SMMC7721 cells after 48 hrs by MTT assay	[PMID: 30151093]
SMMC-7721	IC₅₀	9.95 μM Compound: Amonafide	Cytotoxicity against human SMMC7721 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity against human SMMC7721 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 28177238]
SW-620	IC₅₀	0.32 μM Compound: Amonafide	Cytotoxicity against human SW620 cells after 3 days by MTT assay Cytotoxicity against human SW620 cells after 3 days by MTT assay	[PMID: 24835201]
T-24	IC₅₀	5.01 μM Compound: Amonafide	Antiproliferative activity against human T-24 cells by MTT assay Antiproliferative activity against human T-24 cells by MTT assay	[PMID: 33109400]
T-24	IC₅₀	7.23 μM Compound: Amonafide	Antiproliferative activity against human T24 cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human T24 cells assessed as cell growth inhibition by MTT assay	[PMID: 36894107]
U-373MG ATCC	IC₅₀	3.5 μM Compound: 1, amonafide	Antiproliferative activity against U373MG cells after 72 hrs by MTT assay Antiproliferative activity against U373MG cells after 72 hrs by MTT assay	[PMID: 17658777]
U-87MG ATCC	IC₅₀	2.8 μM Compound: 3, ST	Cytotoxicity against human U87MG cells after 72 hrs by MTT assay Cytotoxicity against human U87MG cells after 72 hrs by MTT assay	[PMID: 22138307]
UACC-375	IC₅₀	2031 nM Compound: 1	Anti-tumor activity against human UACC375 melanoma cell lines by using MTT assay Anti-tumor activity against human UACC375 melanoma cell lines by using MTT assay	[PMID: 7699715]
UACC-375	IC₅₀	2031 nM Compound: 1	Tested for inhibitory activity against human tumor cell line UA375 of melanoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line UA375 of melanoma using sulforhodamine B assay.	[PMID: 10956214]
V79	IC₅₀	70.3 μM Compound: B2	Cytotoxicity against V79 cells in hypoxic condition after 24 hrs by MTT assay Cytotoxicity against V79 cells in hypoxic condition after 24 hrs by MTT assay	[PMID: 17331719]
WiDr	IC₅₀	1100 nM Compound: 1	Tested for inhibitory activity against a sensitive strain of human colon carcinoma WiDr using sulforhodamine B assay (it is MRP positive but non-P-glycoprotein). Tested for inhibitory activity against a sensitive strain of human colon carcinoma WiDr using sulforhodamine B assay (it is MRP positive but non-P-glycoprotein).	[PMID: 10956214]
WiDr	IC₅₀	2200 nM Compound: 1	Tested for inhibitory activity against a multi drug resistant (MDR) strain of human colon carcinoma WiDr using sulforhodamine B assay (it is MRP positive but non-P-glycoprotein). Tested for inhibitory activity against a multi drug resistant (MDR) strain of human colon carcinoma WiDr using sulforhodamine B assay (it is MRP positive but non-P-glycoprotein).	[PMID: 10956214]
WM 266-4	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human WM266.4 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human WM266.4 cells preincubated for 6 hrs followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
WM 266-4	GI₅₀	> 25 μM Compound: AM	Growth inhibition of human WM266.4 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay Growth inhibition of human WM266.4 cells preincubated for 6 hrs cotreated with chlorambucil followed compound washout measured after 24 hrs by XTT assay	[PMID: 28710962]
WM 266-4	IC₅₀	6.92 μM Compound: Amonafide	Cytotoxicity against human WM 266-4 cells assessed as cell viability measured after 96 hrs by XTT assay Cytotoxicity against human WM 266-4 cells assessed as cell viability measured after 96 hrs by XTT assay	[PMID: 34507011]

体外研究
(In Vitro)

Amonafide 是一种拓扑异构酶 II 抑制剂和 DNA 嵌入剂，可通过阻断 Topo II 与 DNA 的结合来诱导细胞凋亡信号^[1]。Amonafide 产生蛋白质相关的 DNA 裂解、单链断裂 (SSB) 以及 DPC 和 DNA 双链裂解。Amonafide (Nafidimide，400 nM-2.4 μM) 以剂量依赖性方式降低白血病细胞系的集落形成能力^[2]。Amonafide (0.05-0.4 μg/mL) 减少多种肿瘤生长。然而，与标准药物 (5-氟尿嘧啶、丝裂霉素 C、顺铂和依托泊苷) 相比，Amonafide 仅对 12% 的肿瘤具有活性，而标准药物对人类骨髓抑制范围内超过 40% 的肿瘤具有活性^[3]。Amonafide 抑制 HT-29、HeLa 和 PC-3 细胞系的生长，IC₅₀ 分别为 4.67、2.73 和 6.38 μM^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

283.33

Formula

C₁₆H₁₇N₃O₂

CAS 号

69408-81-7

性状

固体

颜色

Light yellow to yellow

中文名称

氨萘非特

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 41.67 mg/mL (147.07 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.5295 mL	17.6473 mL	35.2945 mL
5 mM	0.7059 mL	3.5295 mL	7.0589 mL
10 mM	0.3529 mL	1.7647 mL	3.5295 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.82 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (7.34 mM); 悬浊液; 超声助溶

此方案可获得 2.08 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.92%

选择批次：

Data Sheet (646 KB) SDS (392 KB)

COA (291 KB) HNMR (200 KB) LCMS (122 KB) Others (70 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Allen SL, et al. Amonafide: a potential role in treating acute myeloid leukemia. Expert Opin Investig Drugs. 2011 Jul;20(7):995-1003. [Content Brief]

[2]. Andersson BS, et al. In vitro toxicity and DNA cleaving capacity of benzisoquinolinedione (nafidimide; NSC 308847) in human leukemia. Cancer Res. 1987 Feb 15;47(4):1040-4. [Content Brief]

[3]. Ajani JA, et al. In vitro activity of amonafide against primary human tumors compared with the activity of standard agents. Invest New Drugs. 1988 Jun;6(2):79-85. [Content Brief]

Cell Assay ^[2]	In experiments measuring survival following 1 h drug treatments, 2 × 10⁶cells are resuspended in 2 mL warm (37°C) HBSS with 5% PCS; the appropriate drug (Amonafide) level is attained with the addition of less than 50 μL. Cells are incubated for 60 min at 37°C after which 10 mL ice cold PBS is added. The cells are then centrifuged at 200 × g for 10 min at 4°C. The wash is repeated once and the cells are resuspended in HBSS with 5% PCS and added to the agar-medium mixture for assessment of surviving clonogenic cells^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Allen SL, et al. Amonafide: a potential role in treating acute myeloid leukemia. Expert Opin Investig Drugs. 2011 Jul;20(7):995-1003. [Content Brief] [2]. Andersson BS, et al. In vitro toxicity and DNA cleaving capacity of benzisoquinolinedione (nafidimide; NSC 308847) in human leukemia. Cancer Res. 1987 Feb 15;47(4):1040-4. [Content Brief] [3]. Ajani JA, et al. In vitro activity of amonafide against primary human tumors compared with the activity of standard agents. Invest New Drugs. 1988 Jun;6(2):79-85. [Content Brief]

Cell Assay
^[2]

In experiments measuring survival following 1 h drug treatments, 2 × 10⁶cells are resuspended in 2 mL warm (37°C) HBSS with 5% PCS; the appropriate drug (Amonafide) level is attained with the addition of less than 50 μL. Cells are incubated for 60 min at 37°C after which 10 mL ice cold PBS is added. The cells are then centrifuged at 200 × g for 10 min at 4°C. The wash is repeated once and the cells are resuspended in HBSS with 5% PCS and added to the agar-medium mixture for assessment of surviving clonogenic cells^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Allen SL, et al. Amonafide: a potential role in treating acute myeloid leukemia. Expert Opin Investig Drugs. 2011 Jul;20(7):995-1003. [Content Brief]

[2]. Andersson BS, et al. In vitro toxicity and DNA cleaving capacity of benzisoquinolinedione (nafidimide; NSC 308847) in human leukemia. Cancer Res. 1987 Feb 15;47(4):1040-4. [Content Brief]

[3]. Ajani JA, et al. In vitro activity of amonafide against primary human tumors compared with the activity of standard agents. Invest New Drugs. 1988 Jun;6(2):79-85. [Content Brief]

Amonafide 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5295 mL	17.6473 mL	35.2945 mL	88.2363 mL
	5 mM	0.7059 mL	3.5295 mL	7.0589 mL	17.6473 mL
	10 mM	0.3529 mL	1.7647 mL	3.5295 mL	8.8236 mL
	15 mM	0.2353 mL	1.1765 mL	2.3530 mL	5.8824 mL
	20 mM	0.1765 mL	0.8824 mL	1.7647 mL	4.4118 mL
	25 mM	0.1412 mL	0.7059 mL	1.4118 mL	3.5295 mL
	30 mM	0.1176 mL	0.5882 mL	1.1765 mL	2.9412 mL
	40 mM	0.0882 mL	0.4412 mL	0.8824 mL	2.2059 mL
	50 mM	0.0706 mL	0.3529 mL	0.7059 mL	1.7647 mL
	60 mM	0.0588 mL	0.2941 mL	0.5882 mL	1.4706 mL
	80 mM	0.0441 mL	0.2206 mL	0.4412 mL	1.1030 mL
	100 mM	0.0353 mL	0.1765 mL	0.3529 mL	0.8824 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amonafide69408-81-7AS1413氨萘非特AS 1413AS-1413TopoisomeraseInhibitorinhibitorinhibit

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Amonafide (Synonyms: 氨萘非特; AS1413)

Customer Review

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MCE 顾客使用本产品发表的 3 篇科研文献

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H

J

L

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Q

S

T

X

Y

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