2. Apoptosis Metabolic Enzyme/Protease
  3. Glutathione Peroxidase Ferroptosis
  4. ML-210

ML-210 是一种选择性，共价的谷胱甘肽过氧化物酶 4 (GPX4) 抑制剂，EC50 为 30 nM。ML-210 结合 GPX4 硒代半胱氨酸残基。ML210 具有抗癌活性。

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ML-210 Chemical Structure

ML-210 Chemical Structure

CAS No. : 1360705-96-9

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥940
In-stock
1 mg ￥409
In-stock
5 mg ￥900
In-stock
10 mg ￥1600
In-stock
25 mg ￥3000
In-stock
50 mg ￥4800
In-stock
100 mg 现货 询价
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

ML-210 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

ML-210 is a selective and covalent glutathione peroxidase 4 (GPX4) inhibitor with an EC50 of 30 nM. ML-210 binds the GPX4 selenocysteine residue. ML-210 has anti-cancer activity[1][2].

IC50 & Target

Glutathione Peroxidase 4 (GPX4)[1]
细胞效力
(Cellular Effect)
Cell Line Type Value Description References
BJ IC50
107 nM
Compound: 2y
Cytotoxicity against human BJ cells expressing HRAS G12V mutant with alternative oncogenic constructs after 48 hrs by alamar blue assay
Cytotoxicity against human BJ cells expressing HRAS G12V mutant with alternative oncogenic constructs after 48 hrs by alamar blue assay
[PMID: 22297109]
HCT-116 IC50
> 1 μM
Compound: ML-210
Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
HeLa IC50
0.253 μM
Compound: ML-210
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
HT-1080 IC50
0.022 μM
Compound: ML-210
Cytotoxicity against human HT-1080 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against human HT-1080 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
MCF7 IC50
> 20 μM
Compound: ML210
Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs in presence of Fer-1 by CCK8 assay
Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs in presence of Fer-1 by CCK8 assay
[PMID: 33725632]
MCF7 IC50
0.076 μM
Compound: ML210
Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 assay
[PMID: 33725632]
MDA-MB-231 IC50
0.02 μM
Compound: ML-210
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
MDA-MB-468 IC50
0.066 μM
Compound: ML-210
Cytotoxicity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
MEF IC50
0.016 μM
Compound: ML-210
Cytotoxicity against mouse MEF cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against mouse MEF cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
NCI-H522 IC50
0.044 μM
Compound: ML-210
Cytotoxicity against human NCI-H522 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against human NCI-H522 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
NCI-H522 IC50
34.515 μM
Compound: ML-210
Cytotoxicity against human NCI-H522 cells assessed as reduction in cell viability incubated for 3 days in presence of ferroptosis inhibitor, liproxstatin-1 by methylene blue staining based analysis
Cytotoxicity against human NCI-H522 cells assessed as reduction in cell viability incubated for 3 days in presence of ferroptosis inhibitor, liproxstatin-1 by methylene blue staining based analysis
[PMID: 35984756]
U2OS IC50
0.822 μM
Compound: ML-210
Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
WI-38 IC50
0.06 μM
Compound: ML-210
Cytotoxicity against human WI-38 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
Cytotoxicity against human WI-38 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis
[PMID: 35984756]
体外研究
(In Vitro)

ML-210 在一组 821 种癌细胞系 (WM88、LOX-IMVI、CJM、U257、CAKI2、A498、HT1080、MC38、PANC02) 中表现出细胞杀伤活性。ML-210 是一种前药，需要细胞激活才能结合 GPX4[1]。
ML-210 对 BJeLR (HRASV12)、BJeH-LT 的 IC50 分别为 71 nM、272 nM 和 107 nM (无 HRASV12) 和 DRD 细胞系，分别为[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

ML-210 相关抗体:
分子量

475.32

Formula

C22H20Cl2N4O4
CAS 号
性状

固体

颜色

White to light yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 25 mg/mL (52.60 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1038 mL 10.5192 mL 21.0385 mL
5 mM 0.4208 mL 2.1038 mL 4.2077 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (5.26 mM); 悬浊液; 超声助溶

    此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (4.38 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.87%

COA (288 KB) LCMS (264 KB)

产品使用指南 (1538 KB)
参考文献

ML-210 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1038 mL 10.5192 mL 21.0385 mL 52.5961 mL
5 mM 0.4208 mL 2.1038 mL 4.2077 mL 10.5192 mL
10 mM 0.2104 mL 1.0519 mL 2.1038 mL 5.2596 mL
15 mM 0.1403 mL 0.7013 mL 1.4026 mL 3.5064 mL
20 mM 0.1052 mL 0.5260 mL 1.0519 mL 2.6298 mL
25 mM 0.0842 mL 0.4208 mL 0.8415 mL 2.1038 mL
30 mM 0.0701 mL 0.3506 mL 0.7013 mL 1.7532 mL
40 mM 0.0526 mL 0.2630 mL 0.5260 mL 1.3149 mL
50 mM 0.0421 mL 0.2104 mL 0.4208 mL 1.0519 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML-2101360705-96-9ML210ML 210Glutathione PeroxidaseFerroptosisCovalentglutathioneperoxidaseGPX4selenocysteineresidueanti-cancerInhibitorinhibitorinhibit

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