IL-17RA 蛋白, Human (HEK293, hFc)

IL-17RA (Interleukin 17 receptor A), a receptor for IL-17A and IL-17F, is a type I membrane glycoprotein. It is expressed ubiquitously and exhibits a broad tissue distribution, and plays a role in many inflammatory and autoimmune diseases. IL-17RA is a common co-receptor subunit for other members of the IL-17 family. IL-17RA associates with IL-17RC to form a signaling receptor complex for IL-17A and IL-17F^[1][2]. IL-17RA Protein, Human (HEK 293, hFc) is produced in HEK293 cells with a C-Terminal hFc-tag.

白细胞介素 17 受体 A (IL-17RA)，也称为 CD217，是一种结合白细胞介素 17 的细胞因子受体。IL-17RA 是由活化的 T 淋巴细胞分泌的促炎细胞因子。IL-17受体家族由IL-17RA至IL-17RE五个成员组成。IL-17RA 亚基普遍存在，是 IL-17 家族其他成员的常见共受体亚基。IL-17RA 基因定位于人类染色体 22q11.1，广泛表达并表现出广泛的组织分布^[1][2]。
人 IL17RA 蛋白的酸序列在小鼠和大鼠 IL-17A 蛋白之间具有较低的同源性。
IL-17A 是原型 T 辅助细胞 17 (Th17) 细胞因子，在粘膜表面的宿主防御中发挥着重要作用，同时也充当重要的炎症介质，参与许多自身免疫性疾病的发病机制，包括类风湿性关节炎（RA）。IL-17RA 与 IL-17RC 配对，以共价同二聚体形式结合 IL-17A 或 IL-17F，也以异二聚体形式结合 IL-17A/IL-17F。IL-17RA 可提供针对病原体感染的保护，因为它在宿主防御机制中发挥着重要作用。IL-17A 通过 IL-17 受体 (IL-17R) 复合物激活下游细胞信号传导，该复合物由对 IL-17RA 具有更高亲和力的 IL-17A 受体 (IL-17RA) 和 IL-17C 受体 (IL-17RC) 亚基组成。IL-17A/IL-17RA 轴与多种急性和慢性气道和肺部疾病的发病机制有关。然而，IL-17RA 与 IL-17 受体家族其他成员的结合使亲和力转向 IL-17 家族其他成员。事实上，IL-17RA 与 IL-17RB 的组合以共价同二聚体形式结合 IL-17E。此外，IL-17RA还可以与IL-17RE缔合，以同型二聚体形式结合IL-17C。然而，IL-17RA 的参与不是强制性的，因为 IL-17RB 形成同型二聚体，以共价同型二聚体的形式靶向 IL-17B^[1][2][3]。
IL-17RA在类风湿性关节炎等多种炎症和自身免疫性疾病中发挥致病作用。IL-17RA 参与抗菌宿主防御和组织完整性的维持。在感染模型中已证明，中性粒细胞对于宿主防御至关重要，IL-17RA 缺乏会导致趋化因子水平降低和中性粒细胞数量减少，并增加对感染的易感性。此外，IL-17RA 由多种肠道细胞类型表达，针对 IL-17A 的治疗会诱导不良肠道事件，这表明有额外的组织特异性功能。IL17RA 缺陷是导致家族性念珠菌病 5 型 (CANDF5) 的原因。IL17RA 缺乏可能导致慢性皮肤粘膜念珠菌病的风险^[1][2][3][4]。

IL-17RA Protein, Human (10 μg/mL; 72 hours) preincubated with IL-2 plus IL-25 for 30 min before addition to the PBMC cultures, the result shows IL-17RA does not inhibit IL-5 production in response to stimulation by IL-25 plus IL-2^[5].

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 110-200 ng/mL in the presence of 10 ng/mL recombinant human IL-17.

• 
  Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.The ED₅₀ for this effect is 0.1102 μg/mL in the presence of 10 ng/mL recombinant human IL‑17, corresponding to a specific activity is 9074.4 U/mg.

Human

HEK293

C-hFc

Q96F46-1(L33-W320)

23765  [NCBI]

LRLLDHRALVCSQPGLNCTVKNSTCLDDSWIHPRNLTPSSPKDLQIQLHFAHTQQGDLFPVAHIEWTLQTDASILYLEGAELSVLQLNTNERLCVRFEFLSKLRHHHRRWRFTFSHFVVDPDQEYEVTVHHLPKPIPDGDPNHQSKNFLVPDCEHARMKVTTPCMSSGSLWDPNITVETLEAHQLRVSFTLWNESTHYQILLTSFPHMENHSCFEHMHHIPAPRPEEFHQRSNVTLTLRNLKGCCRHQVQIQPFFSSCLNDCLRHSATVSCPEMPDTPEPIPDYMPLW

70-100 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Stéphane Nesmond, et al. Characteristic Pattern of IL-17RA, IL-17RB, and IL-17RC in Monocytes/Macrophages and Mast Cells From Patients With Bullous Pemphigoid. Front Immunol. 2019 Sep 11;10:2107.  [Content Brief]

  [2]. Xun Lin, et al. IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment. Immunity. 2022 Feb 8;55(2):237-253.e8.  [Content Brief]

  [3]. Laura Green, et al. Chronic Candidiasis in Children. Curr Allergy Asthma Rep. 2017 May;17(5):31.  [Content Brief]

  [4]. Jayasri Das Sarma, et al. Functional interleukin-17 receptor A is expressed in central nervous system glia and upregulated in experimental autoimmune encephalomyelitis. J Neuroinflammation. 2009 Apr 28;6:14.  [Content Brief]

  [5]. Erika A Rickel, et al. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J Immunol. 2008 Sep 15;181(6):4299-310.  [Content Brief]