2. Apoptosis
  3. Apoptosis
  4. Sophoridine

Sophoridine  (Synonyms: 槐定碱)

目录号: HY-N1373 纯度: 99.45%
COA 产品使用指南

摩尔计算器

Sophoridine 是一种从豆科植物苦参中分离出来的喹喔啉生物碱。Sophoridine 诱导细胞凋亡 (apoptosis)。Sophoridine 有可能成为胰腺癌的有效，选择性和耐受性良好的候选活性分子。

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Sophoridine Chemical Structure

Sophoridine Chemical Structure

CAS No. : 6882-68-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥310
In-stock
5 mg ￥280
In-stock
10 mg ￥400
In-stock
20 mg ￥600
In-stock
50 mg ￥1150
In-stock
100 mg   询价  
200 mg   询价  

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Customer Review

Other Forms of Sophoridine:

Sophoridine 相关抗体

Top Publications Citing Use of Products

    Sophoridine purchased from MCE. Usage Cited in: Front Oncol. 2021 Feb 24;11:634851.  [Abstract]

    LPS and Sophoridine (20 and 40 μg/mL) triggered the phosphorylation of JNK, ERK, and p38 MAPK in RAW264.7 and THP-1-derived macrophages. RAW264.7 and THP-1 cells are pre-treated with SP600125, and then stimulated with or without Sophoridine (20 and 40 μg/mL).

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Sophoridine is a quinolizidine alkaloid isolated from Leguminous plant Sophora flavescens. Sophoridine induces apoptosis. Sophoridine has the potential to be a novel, potent and selective antitumor agent candidate for pancreatic cancer with well-tolerated toxicity[1].

    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A549 IC50
    > 80 μM
    Compound: 1
    Antiproliferative activity against human A549 cells after 3 days by SRB assay
    Antiproliferative activity against human A549 cells after 3 days by SRB assay
    		[PMID: 26865176]
    A549 IC50
    5836 μM
    Compound: Sophoridine
    Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    		[PMID: 30025344]
    CNE-2 IC50
    5379 μM
    Compound: Sophoridine
    Cytotoxicity against human CNE2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Cytotoxicity against human CNE2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    		[PMID: 30025344]
    HeLa IC50
    4332 μM
    Compound: Sophoridine
    Antiproliferative activity against human HeLa cells by MTT assay
    Antiproliferative activity against human HeLa cells by MTT assay
    		[PMID: 30007563]
    HepG2 IC50
    > 20 μg/mL
    Compound: 1
    Antiproliferative activity against human HepG2 cells assessed as growth inhibition after 48 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells assessed as growth inhibition after 48 hrs by MTT assay
    		[PMID: 24826818]
    HepG2 IC50
    > 80 μM
    Compound: 1
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 25408835]
    HepG2 IC50
    1160 μg/mL
    Compound: Sophoridine
    Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay
    		[PMID: 26299348]
    HepG2 IC50
    4509 μM
    Compound: Sophoridine
    Antiproliferative activity against human HepG2 cells by MTT assay
    Antiproliferative activity against human HepG2 cells by MTT assay
    		[PMID: 30007563]
    HepG2 IC50
    4670 μM
    Compound: Sophoridine
    Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 30125725]
    HepG2 IC50
    4670 μM
    Compound: Sophoridine
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    		[PMID: 30025344]
    KB IC50
    > 80 μM
    Compound: 1
    Antiproliferative activity against human KB cells after 3 days by SRB assay
    Antiproliferative activity against human KB cells after 3 days by SRB assay
    		[PMID: 26865176]
    MCF7 IC50
    > 80 μM
    Compound: 1
    Antiproliferative activity against human MCF7 cells after 3 days by SRB assay
    Antiproliferative activity against human MCF7 cells after 3 days by SRB assay
    		[PMID: 26865176]
    MDA-MB-231 IC50
    > 80 μM
    Compound: 1
    Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay
    Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay
    		[PMID: 26865176]
    体外研究
    (In Vitro)

    Sophoridine (0-500 μM; 48 hours) exhibits remarkable inhibition effects to the growth of human pancreatic, gastric, liver, colon, gallbladder, and prostate carcinoma cells with IC50 values of about 20 μM to 200 μM[1].
    Sophoridine (0-20 μM; 48 hours) increases S phase cell population from 26.23% (control) to 38.67% in Miapaca-2 cells and from 29.56% (control) to 39.16% in PANC-1 cells, about a 1.5-fold and a 1.3-fold increase, respectively[1].
    Sophoridine (0-20 μM; 48 hours) significantly increases bad and bax levels, and decreases bcl-2 and bcl-xl levels in contrast, with a significant increase in Bax/Bcl-2 ratio[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Sophoridine 相关抗体:

    Cell Viability Assay[1]

    Cell Line: Normal cells: IOSE144, HL-7702 and LO2, BEAS-2B, GES-1, HEK 293 T, HPDE, FHC, Human cancer cells: PANC-1, Mapaca-1, hepG2, SGC-7901, CBC-SD,SGC-996,PC-3,MKN-45,MGC-803,Hela and HCT116 cells
    Concentration: 0, 3.9, 7.8, 15.5, 31, 62.5, 125, 250, 500 μM
    Incubation Time: 48 hours
    Result: Exhibited the most potent cytotoxicity to cancer cells.

    Cell Cycle Analysis[1]

    Cell Line: PANC-1 cells; Miapca-2 cells
    Concentration: 20 μM
    Incubation Time: 48 hours
    Result: Led to accumulated population in the S phase.

    Western Blot Analysis[1]

    Cell Line: PANC-1 cells; Miapca-2 cells
    Concentration: 20 μM
    Incubation Time: 48 hours
    Result: Induced the activation of intrinsic apoptosis pathway.
    体内研究
    (In Vivo)

    Sophoridine (intraperitoneal injection; 20 or 40 mg/kg; 21 days) can inhibit the growth of xenograft pancreatic tumors[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: BALB/c homozygous (nu/nu) nude mice[1]
    Dosage: 20 or 40 mg/kg
    Administration: Intraperitoneal injection; 20 or 40 mg/kg; 21 days
    Result: Decreased xenograft pancreatic tumors mass.
    分子量

    248.36

    Formula

    C15H24N2O
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    槐定碱
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 50 mg/mL (201.32 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 4.0264 mL 20.1321 mL 40.2641 mL
    5 mM 0.8053 mL 4.0264 mL 8.0528 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.17 mg/mL (8.74 mM); 澄清溶液

      此方案可获得 ≥ 2.17 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.17 mg/mL (8.74 mM); 澄清溶液

      此方案可获得 ≥ 2.17 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料
    参考文献

    Sophoridine 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.0264 mL 20.1321 mL 40.2641 mL 100.6603 mL
    5 mM 0.8053 mL 4.0264 mL 8.0528 mL 20.1321 mL
    10 mM 0.4026 mL 2.0132 mL 4.0264 mL 10.0660 mL
    15 mM 0.2684 mL 1.3421 mL 2.6843 mL 6.7107 mL
    20 mM 0.2013 mL 1.0066 mL 2.0132 mL 5.0330 mL
    25 mM 0.1611 mL 0.8053 mL 1.6106 mL 4.0264 mL
    30 mM 0.1342 mL 0.6711 mL 1.3421 mL 3.3553 mL
    40 mM 0.1007 mL 0.5033 mL 1.0066 mL 2.5165 mL
    50 mM 0.0805 mL 0.4026 mL 0.8053 mL 2.0132 mL
    60 mM 0.0671 mL 0.3355 mL 0.6711 mL 1.6777 mL
    80 mM 0.0503 mL 0.2517 mL 0.5033 mL 1.2583 mL
    100 mM 0.0403 mL 0.2013 mL 0.4026 mL 1.0066 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sophoridine6882-68-4槐定碱ApoptosisInhibitorinhibitorinhibit

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