Atractylenolide III (Synonyms: 白术内酯III; ICodonolactone; 8β-Hydroxyasterolide)

目录号: HY-N0203 纯度: 99.94%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Atractylenolide III (ICodonolactone) 是白术根茎主要成分，有诱导肺癌细胞凋亡 (apoptosis) 活性。Atractylenolide III 是一种具有口服活性的胃保护剂。

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Atractylenolide III Chemical Structure

CAS No. : 73030-71-4

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥880	In-stock
5 mg	￥800	In-stock
10 mg	￥1250	In-stock
25 mg	￥2500	In-stock
50 mg	￥4000	In-stock
100 mg	现货	询价
200 mg		询价
500 mg		询价

or 大包装询价

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Customer Review

Other Forms of Atractylenolide III:

Atractylenolide III (Standard) 询价

MCE 顾客使用本产品发表的 3 篇科研文献

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生物活性
纯度 & 产品资料
参考文献

生物活性

Atractylenolide III (ICodonolactone) is the main component of Atractylodes rhizome and has the activity of inducing apoptosis in lung cancer cells. Atractylenolide III is an orally active gastroprotective agent^[1]^[2]^[3].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
CHO	IC₅₀	100.7 μM Compound: 1	Inhibition of human SOAT2-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis Inhibition of human SOAT2-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis	[PMID: 32035699]
CHO	IC₅₀	174.9 μM Compound: 1	Inhibition of human SOAT1 mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis Inhibition of human SOAT1 mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis	[PMID: 32035699]
CHO	IC₅₀	187.3 μM Compound: 1	Inhibition of human SOAT2-mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis Inhibition of human SOAT2-mediated [14C]Cholesterol ester synthesis in CHO cells after 6 hrs in presence of [14C]Oleic acid by TLC analysis	[PMID: 32035699]
CHO	IC₅₀	55.1 μM Compound: 1	Inhibition of human SOAT1-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis Inhibition of human SOAT1-mediated [14C]Cholesterol ester biosynthesis in CHO microsomal fraction after 5 mins in presence of [1-14C]oleoyl-CoA by TLC analysis	[PMID: 32035699]
CHO-K1	IC₅₀	> 195 μM Compound: 1	Inhibition of [14C]triglyceride biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method Inhibition of [14C]triglyceride biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method	[PMID: 32035699]
CHO-K1	IC₅₀	73.5 μM Compound: 1	Inhibition of [14C]cholesterol ester biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method Inhibition of [14C]cholesterol ester biosynthesis in CHOK1 cells using [14C]Oleic acid as substrate after 6 hrs by TLC method	[PMID: 32035699]
PC-12	EC₅₀	36.3 μM Compound: 16; 19	Neuroprotective activity against glutamate-induced neuronal cell death in rat PC12 cells assessed as increase in cell viability pretreated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay Neuroprotective activity against glutamate-induced neuronal cell death in rat PC12 cells assessed as increase in cell viability pretreated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay	[PMID: 29775304]
RAW264.7	IC₅₀	> 100 μM Compound: 8	Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 24 hrs in presence of LPS by colorimetric analysis Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 24 hrs in presence of LPS by colorimetric analysis	[PMID: 27865705]

体外研究
(In Vitro)

Atractylenolide III (1-100 μM, 48 h) 诱导 A549 细胞凋亡，并诱导 caspase-3 和 caspase-9 激活以及 PARP 裂解^[1]。
Atractylenolide III (1-100 μM，72 小时) 抑制 HUVEC 的增殖和血管生成^[1]。
Atractylenolide III (1-100 μM) 抑制 HMC-1 细胞中胸腺基质淋巴细胞生成素 (TSLP) 诱导的促炎细胞因子 (IL-6、IL-1b、TNF-α 和 IL-8) 的产生^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	A549
Concentration:	1, 10, 100 μM
Incubation Time:	24 h, 48 h
Result:	Increased active form of caspase-9, caspase-3, and the cleavage of PARP at 48 h. Increased the expression of proapoptotic protein bax, and induced AIF translocation to the nucleus at 24 h.

体内研究
(In Vivo)

Atractylenolide III (5 和 10 mg/kg，口服) 具有胃保护作用，可减少大鼠中 70% 乙醇诱发的胃溃疡^[2]。
Atractylenolide III (30 mg/kg，口服灌胃 14 天或 28 天) 可减少 Lipopolysaccharides (HY-D1056) 和慢性不可预测轻度应激 (CUMS) 诱导的大鼠抑郁模型中的抑郁和焦虑样行为^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	70% ethanol-induced gastric ulcers in rats^[2]
Dosage:	5 and 10 mg/kg
Administration:	p.o.
Result:	Inhibited gastric ulcer formation and the necrotic erosion of gastric mucosa. Downregulated the MMP-2/9 expression by activating the TIMP-2 and TIMP-1 expressions.

分子量

248.32

Formula

C₁₅H₂₀O₃

CAS 号

73030-71-4

性状

固体

颜色

Off-white to light yellow

中文名称

白术内酯 III

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (201.35 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.0271 mL	20.1353 mL	40.2706 mL
5 mM	0.8054 mL	4.0271 mL	8.0541 mL
10 mM	0.4027 mL	2.0135 mL	4.0271 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.07 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.07 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.07 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.94%

选择批次：

Data Sheet (641 KB) SDS (393 KB)

COA (290 KB) LCMS (90 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Kang TH, et al. Atractylenolide III, a sesquiterpenoid, induces apoptosis in human lung carcinoma A549 cells via mitochondria-mediated death pathway. Food Chem Toxicol. 2011 Feb;49(2):514-9. [Content Brief]

[2]. Wang KT, et al. Gastroprotective activity of atractylenolide III from Atractylodes ovata on ethanol-induced gastric ulcer in vitro and in vivo. J Pharm Pharmacol. 2010 Mar;62(3):381-8. [Content Brief]

[3]. Kim HK, et al. Toxicity of atractylon and atractylenolide III Identified in Atractylodes ovata rhizome to Dermatophagoides farinae and Dermatophagoides pteronyssinus. J Agric Food Chem. 2007 Jul 25;55(15):6027-31. [Content Brief]

[4]. Yoou MS, et al. Ameliorative effect of atractylenolide III in the mast cell proliferation induced by TSLP. Food Chem Toxicol. 2017 Aug;106(Pt A):78-85. [Content Brief]

[5]. Zhou Y, et al. Atractylenolide III reduces depressive- and anxiogenic-like behaviors in rat depression models. Neurosci Lett. 2021 Aug 10;759:136050. [Content Brief]

Atractylenolide III 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0271 mL	20.1353 mL	40.2706 mL	100.6765 mL
	5 mM	0.8054 mL	4.0271 mL	8.0541 mL	20.1353 mL
	10 mM	0.4027 mL	2.0135 mL	4.0271 mL	10.0677 mL
	15 mM	0.2685 mL	1.3424 mL	2.6847 mL	6.7118 mL
	20 mM	0.2014 mL	1.0068 mL	2.0135 mL	5.0338 mL
	25 mM	0.1611 mL	0.8054 mL	1.6108 mL	4.0271 mL
	30 mM	0.1342 mL	0.6712 mL	1.3424 mL	3.3559 mL
	40 mM	0.1007 mL	0.5034 mL	1.0068 mL	2.5169 mL
	50 mM	0.0805 mL	0.4027 mL	0.8054 mL	2.0135 mL
	60 mM	0.0671 mL	0.3356 mL	0.6712 mL	1.6779 mL
	80 mM	0.0503 mL	0.2517 mL	0.5034 mL	1.2585 mL
	100 mM	0.0403 mL	0.2014 mL	0.4027 mL	1.0068 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Atractylenolide III73030-71-4ICodonolactone 8β-Hydroxyasterolide白术内酯 IIIApoptosisgastroprotectiveanxiogenicA549HUVECInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Atractylenolide III (Synonyms: 白术内酯III; ICodonolactone; 8β-Hydroxyasterolide)

Customer Review

Other Forms of Atractylenolide III:

MCE 顾客使用本产品发表的 3 篇科研文献