2. 诱导疾病模型产品 Immunology/Inflammation
  3. 免疫与炎症疾病模型 消化系统疾病模型 Toll-like Receptor (TLR)
  4. 关节炎模型 肝脏炎症模型 肝脏疾病模型
  5. Lipopolysaccharides, from E. coli O55:B5

Lipopolysaccharides, from E. coli O55:B5  (Synonyms: 脂多糖; LPS)

目录号: HY-D1056
COA 产品使用指南

摩尔计算器

Lipopolysaccharides, from E. coli O55:B5 是从 E. coli O55:B5 中提取的内毒素，由一个抗原 O-特异性链、一个核心寡糖和脂质 A 构成。Lipopolysaccharides, from E. coli O55:B5 激活免疫细胞的 TLR-4。Lipopolysaccharides, from E. coli O55:B5 可以引起大鼠体温变化，且具有剂量和血清型特异性，Lipopolysaccharides, from E. coli O55:B5 导致大鼠体温多相性和非剂量依赖性升高。

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Lipopolysaccharides, from E. coli O55:B5 Chemical Structure

Lipopolysaccharides, from E. coli O55:B5 Chemical Structure

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Lipopolysaccharides, from E. coli O55:B5 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 223 篇科研文献

Proliferation Assay
IF
WB
RT-PCR

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2022 May 23;e2105650.  [Abstract]

    In LPS (1 µg/mL; 24 h)-stimulated macrophages, Representative fluorescence images of macrophage phenotypes after incubation with different pretreated neutrophils; iNOS (red), CD206 (green), and nuclei (blue).

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2022 May 23;e2105650.  [Abstract]

    Western blot analysis of the phenotypic markers in LPS (1 µg/mL; 24 h)-stimulated macrophages.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: Biomater Res. 2022 Apr 25;26(1):15.  [Abstract]

    HUVECs ae induced with LPS 100 ng/mL for 24 h, then treated with PLCL-N, MPSS-loaded with 0.2 mg, 0.4 mg, 0.6 mg, 0.8 mg and 1 mg for another 24 h.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: Sens Actuators B Chem. 15 December 2022, 132707.

    LPS is used as an external stimulus to induce •OH generation in the A549 and HeLa cell lines. Co-localization fluorescence imaging in A549 and HeLa cells using MTG and PY. Cells were incubated with LPS (10.0 μg/mL) for 24 h and then stained with MTG (500 nM) and PY (5.0 μM) for 30 min.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Jan 21;13(1):73.  [Abstract]

    Effect of NKAP on the U87MG cell sensitivity to Erastin (10 μM), iFSP1 (100 μM), SAS (500 μM), Rotenone (2.5 μM), 17-DMAG (300 nM), Staurosporine (1.5 μM), TMZ (200 μM), β-lapachone (2 μM), H2O2 (1‰), LPS (200 μg/mL), and Rapamycin (300 nM). All drug treatments are for 24 h.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: Elife. 2022 May 4;11:e76707.  [Abstract]

    qPCR analysis for the expression of indicated genes in D40 fVBOrs treated with lipopolysaccharide (LPS) (500 ng/mL; for 72 hr) without or with PLX5622 2 μM using DMSO as vehicle control.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: Front Immunol. 2022 Jun 10;13:859806.  [Abstract]

    BMDMs are planted on XF24-well seahorse plates at the density of 5 × 104 cells per well and stimulated with 100 ng/mL LPS and 20 ng/mL hIFN-γ for 48 hours.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: Adv Funct Mater. 10 March 2022.

    The effect of CaNP on BMDM recruitment of T cells. Left panel: BMDM-M2 treated with CaNP (35 µg/mL) and LPS (50 ng/mL)/IFN-γ (20 ng/mL) for 48 h are in the lower compartment. CD8+ T cells are in the upper compartment. Right panel: representative images of BMDM recruitment of T cells. CD8+ T cells were labeled with FITC.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: J Med Virol. 2020 Aug 10.  [Abstract]

    The results show Apigenin significantly decreased 3pRNA‐ but not poly I:C‐ and LPS (0.5 μg/mL)‐induced A549 cell death.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MCE. Usage Cited in: J Control Release. 2020 Jan 28;320:304-313.  [Abstract]

    Cells were differentiated to macrophages with PMA and then treated with 1 μg/mL LPS for 3 h.

    查看 Toll-like Receptor (TLR) 亚型特异性产品:

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    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Lipopolysaccharides, from E. coli O55:B5 is an endotoxin extracted from E. coli O55:B5, consisting of an antigen-specific chain, A core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O55:B5 activates TLR-4 of immune cells. Lipopolysaccharides, from E. coli O55:B5 can induce the change of body temperature in rats with dose and serotype specificity. Lipopolysaccharides, from E. coli O55:B5 caused a heterogeneous and dose-independent increase in body temperature in rats[1][2][3][4][5].

    IC50 & Target

    TLR4

     

    体外研究
    (In Vitro)

    Lipopolysaccharides (10–80 μg/mL) 选择性地减少 THir (酪氨酸羟化酶免疫反应) 细胞并增加培养基中白细胞介素 1β (IL-1β) 和肿瘤坏死因子-α (TNF-α) 以及亚硝酸盐 (硝酸盐指数) 的水平氧化物 (NO) 的产生[5]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Lipopolysaccharides, from E. coli O55:B5 相关抗体:
    体内研究
    (In Vivo)

    诱导心脏功能障碍[8]

    致病原理
    心脏功能障碍 (心脏炎症) 是脂多糖 (LPS) 诱导脓毒症的常见并发症，其原因是严重的炎症反应。LPS 还可引起心脏收缩功能恶化，心脏脂质过氧化增加，导致心脏功能障碍。
    具体造模方法
    Rat[8]: male • Wistar male rats • 6-week-old
    Administration: 10 mg/kg • ip for single dose • collected cardiac tissues and serums 24 hr after LPS-injection.
    Note
    /
    造模成功指标
    表型变化：心功能的左心室收缩末期容积 (LVESV，%) 和左心室舒张末期容积 (LVEDV，%) 显著升高。
    分子层面：血清中 CK-MB、LDH、AST 水平↑，TLR4/NF-κB 通路受抑制。
    拮抗产品 Ferrostatin-1 (HY-100579)[7]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female and male CD1 mice[3]
    Dosage: 1.5mg/kg
    Administration: Intraperitoneal injection, once
    Result: Induced sickness behavior in all mice, but adult mice displayed more sickness than pubertal mice and adult males remained sick for a longer period of time than adult females.
    Caused a decrease in body temperature for all mice, but this decrease was greatest in adult males.
    Increased pro- and anti-inflammatory cytokines at various levels in pubertal and adult male and female mice, resulted in age and sex differences in cytokine concentrations following immune challenge.
    Only adult males and females treated with LPS displayed significantly more IL-6 than their saline controls, and pubertal males and females and adult females displayed significantly more IL-10 than their saline controls.
    All the mice displayed significantly more IL-12 and TNF-α than their saline controls.
    Clinical Trial
    性状

    固体

    颜色

    White to off-white

    中文名称

    脂多糖,来源于大肠杆菌O55:B5
    结构分类
    初始来源

    surface of Gram-negative bacteria
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 5 mg/mL (超声助溶)

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.43 mg/mL; 澄清溶液

      此方案可获得 ≥ 1.43 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 14.3 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 8.33 mg/mL; 澄清溶液; 超声助溶 (<60°C)

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    COA (255 KB)

    产品使用指南 (1538 KB)
    参考文献

    Lipopolysaccharides, from E. coli O55:B5 相关分类

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Lipopolysaccharides, from E. coli O55:B5LPS脂多糖,来源于大肠杆菌O55:B5Toll-like Receptor (TLR)EscherichiacoliO55:B5endotoxinGram-negativeimmunogenic antigenimmuneTNF-αhypothermiacytokinesInhibitorinhibitorinhibit

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