Cinnamic acid (Synonyms: 肉桂酸; 3-Phenylacrylic acid; β-Phenylacrylic acid)

目录号: HY-N0610A 纯度: 99.99%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Cinnamic acid 在癌症干预中有潜在的用途，其对胶质母细胞瘤，黑色素瘤，前列腺癌，肺癌细胞的 IC₅₀ 值为 1-4.5 mM。

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Cinnamic acid Chemical Structure

CAS No. : 621-82-9

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥550	In-stock
100 mg	￥500	In-stock
200 mg		询价
500 mg		询价

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Cinnamic acid has potential use in cancer intervention, with IC₅₀s of 1-4.5 mM in glioblastoma, melanoma, prostate and lung carcinoma cells.

IC₅₀ & Target

Human Endogenous Metabolite

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-375	IC₅₀	64 μM Compound: 16a	Anticancer activity against human A375 cells after 48 hrs by MTT assay Anticancer activity against human A375 cells after 48 hrs by MTT assay	[PMID: 22136907]
A549	IC₅₀	3.54 mM Compound: Cinnamic acid	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 31336310]
A549	IC₅₀	50.18 μM Compound: CA	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as decrease in cell viability after 24 hrs by MTT assay	10.1039/C6MD00178E
A549	IC₅₀	87 μM Compound: 16a	Anticancer activity against human A549 cells after 48 hrs by MTT assay Anticancer activity against human A549 cells after 48 hrs by MTT assay	[PMID: 22136907]
ACHN	IC₅₀	91 μM Compound: 16a	Anticancer activity against human ACHN cells after 48 hrs by MTT assay Anticancer activity against human ACHN cells after 48 hrs by MTT assay	[PMID: 22136907]
BALB/3T3	IC₅₀	671.4 μM Compound: 11	Growth inhibition of BALB/c mouse cloned 3T3/A31 cells after 72 hrs by nigrosin assay Growth inhibition of BALB/c mouse cloned 3T3/A31 cells after 72 hrs by nigrosin assay	[PMID: 10096863]
BMDM	IC₅₀	> 100 μM Compound: trans-cinnamic acid	Inhibition of M-CSF/RANKL-induced osteoclast differentiation in C57BL/6 mouse bone marrow macrophage assessed as reduction in multinucleated TRAP+ cells incubated for 6 days with fresh media replacement on day 3 and measured on day 6 by TRAP staining-base Inhibition of M-CSF/RANKL-induced osteoclast differentiation in C57BL/6 mouse bone marrow macrophage assessed as reduction in multinucleated TRAP+ cells incubated for 6 days with fresh media replacement on day 3 and measured on day 6 by TRAP staining-base	[PMID: 31257875]
BV-2	EC₅₀	> 10 μM Compound: CA	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced microglial activation after 24 hrs by Griess reagent based assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced microglial activation after 24 hrs by Griess reagent based assay	[PMID: 29407994]
BV-2	IC₅₀	> 100 μM Compound: 22	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 28911817]
DLD-1	IC₅₀	0.685 μM Compound: 25, IWR-1	Inhibition of tankyrase in human DLD1 cells assessed as reduction in Wnt activity after 24 hrs by TCF-luciferase reporter gene assay Inhibition of tankyrase in human DLD1 cells assessed as reduction in Wnt activity after 24 hrs by TCF-luciferase reporter gene assay	[PMID: 25299683]
HeLa	IC₅₀	24.81 μM Compound: CA	Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 24 hrs by MTT assay	10.1039/C6MD00178E
HT-144	IC₅₀	1.07 mM Compound: Cinnamic acid	Cytotoxicity against human HT144 cells after 48 hrs by MTT assay Cytotoxicity against human HT144 cells after 48 hrs by MTT assay	[PMID: 31336310]
HT-144	IC₅₀	2.4 mM Compound: Cinnamic acid	Cytotoxicity against human HT144 cells after 24 hrs by MTT assay Cytotoxicity against human HT144 cells after 24 hrs by MTT assay	[PMID: 31336310]
HT-22	EC₅₀	> 10 μM Compound: CA	Neuroprotective activity against IAA-induced ischemia in mouse HT22 cells assessed as increase in cell viability cotreated with IAA for 2 hrs followed by IAA wash out measured after 24 hrs by MTT assay Neuroprotective activity against IAA-induced ischemia in mouse HT22 cells assessed as increase in cell viability cotreated with IAA for 2 hrs followed by IAA wash out measured after 24 hrs by MTT assay	[PMID: 29407994]
HT-29	IC₅₀	114 μM Compound: 16a	Anticancer activity against human HT-29 cells after 48 hrs by MTT assay Anticancer activity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 22136907]
MCF7	IC₅₀	108 μM Compound: 16a	Anticancer activity against human MCF7 cells after 48 hrs by MTT assay Anticancer activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 22136907]
MIA PaCa-2	IC₅₀	1.33 mM Compound: Cinnamic acid	Cytotoxicity against human MIAPaCa2 cells after 48 hrs by MTT assay Cytotoxicity against human MIAPaCa2 cells after 48 hrs by MTT assay	[PMID: 31336310]
NCI-H460	IC₅₀	2.1 mM Compound: Cinnamic acid	Cytotoxicity against human H460 cells after 48 hrs by MTT assay Cytotoxicity against human H460 cells after 48 hrs by MTT assay	[PMID: 31336310]
Oocyte	IC₅₀	34.6 μM Compound: Cin-COOH	Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique	[PMID: 23682762]
RAW264.7	GI₅₀	6547 μM Compound: 1d	Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay	10.1039/C3MD00251A

体外研究
(In Vitro)

用 Cinnamic acid (CINN) 处理上皮和神经外胚层起源的各种肿瘤细胞，在暴露 3 天后会导致剂量依赖性生长抑制。导致肿瘤细胞增殖减少 50% 的抑制浓度 (IC₅₀) 在 1.2 到 4.5 mM 之间。还表明，需要 20 mM Cinnamic acid 才能在 FS4 细胞中产生 IC₅₀，即比肿瘤细胞高 5 到 20 倍。除了抑制肿瘤细胞增殖外，Cinnamic acid 还会引起与黑素细胞分化一致的形态学变化。在用 5 mM Cinnamic acid 处理 5 天内，黑色素瘤 1011 细胞出现增大，细胞质与细胞核的比例显著增加，细胞骨架组织良好，形成长树突状突起并变得高度黑色素化。Cinnamic acid 处理的黑色素瘤 1011、A375 (M) 和 SKMEL28 细胞降解和穿过组织屏障的能力的变化是通过使用带有基质胶涂层过滤器的改良博伊登室的体外侵袭测定来评估的。用 Cinnamic acid 连续处理 3 天后，在 3 个测试的肿瘤细胞系中观察到侵袭能力的剂量依赖性损失。用 5 mM Cinnamic acid 处理可导致 75-95% 的侵袭性损失^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

148.16

Formula

C₉H₈O₂

CAS 号

621-82-9

性状

固体

颜色

White to off-white

结构分类

初始来源

微生物

内源性代谢物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

Ethanol 中的溶解度 : ≥ 50 mg/mL (337.47 mM)

DMSO 中的溶解度 : 50 mg/mL (337.47 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	6.7495 mL	33.7473 mL	67.4946 mL
5 mM	1.3499 mL	6.7495 mL	13.4989 mL
10 mM	0.6749 mL	3.3747 mL	6.7495 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.87 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.87 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.87 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900 μL 玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.99%

选择批次：

Data Sheet (642 KB) SDS (394 KB)

COA (189 KB) RP-HPLC (256 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Liu L, et al. Cinnamic acid: a natural product with potential use in cancer intervention. Int J Cancer. 1995 Jul 28;62(3):345-50. [Content Brief]

Cell Assay	The cell lines used, established from human malignant tumors, are A549 (lung cancer); PC3(M), Du145, and LNCaP (prostate cancer); A172, U251 (glioblastoma); and SKMEL28, A375(M), 1011 (melanoma). Growth rates are determined by cell counting. Briefly, 5 X10⁴ cells are plated in each well of a 24-well plate, allowed to attach overnight, and treated with compounds (e.g., Cinnamic acid: 2.5, 5, 10, 20, 30 mM) the following day. Cells are grown for 3 days at 37°C in the presence or absence of the drug, then detached with trypsin/EDTA and counted in a Coulter counter. Viability is determined by Trypan-blue exclusion assay^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Liu L, et al. Cinnamic acid: a natural product with potential use in cancer intervention. Int J Cancer. 1995 Jul 28;62(3):345-50. [Content Brief]

Cell Assay

The cell lines used, established from human malignant tumors, are A549 (lung cancer); PC3(M), Du145, and LNCaP (prostate cancer); A172, U251 (glioblastoma); and SKMEL28, A375(M), 1011 (melanoma). Growth rates are determined by cell counting. Briefly, 5 X10⁴ cells are plated in each well of a 24-well plate, allowed to attach overnight, and treated with compounds (e.g., Cinnamic acid: 2.5, 5, 10, 20, 30 mM) the following day. Cells are grown for 3 days at 37°C in the presence or absence of the drug, then detached with trypsin/EDTA and counted in a Coulter counter. Viability is determined by Trypan-blue exclusion assay^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Liu L, et al. Cinnamic acid: a natural product with potential use in cancer intervention. Int J Cancer. 1995 Jul 28;62(3):345-50. [Content Brief]

Cinnamic acid 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	6.7495 mL	33.7473 mL	67.4946 mL	168.7365 mL
	5 mM	1.3499 mL	6.7495 mL	13.4989 mL	33.7473 mL
	10 mM	0.6749 mL	3.3747 mL	6.7495 mL	16.8736 mL
	15 mM	0.4500 mL	2.2498 mL	4.4996 mL	11.2491 mL
	20 mM	0.3375 mL	1.6874 mL	3.3747 mL	8.4368 mL
	25 mM	0.2700 mL	1.3499 mL	2.6998 mL	6.7495 mL
	30 mM	0.2250 mL	1.1249 mL	2.2498 mL	5.6245 mL
	40 mM	0.1687 mL	0.8437 mL	1.6874 mL	4.2184 mL
	50 mM	0.1350 mL	0.6749 mL	1.3499 mL	3.3747 mL
	60 mM	0.1125 mL	0.5625 mL	1.1249 mL	2.8123 mL
	80 mM	0.0844 mL	0.4218 mL	0.8437 mL	2.1092 mL
	100 mM	0.0675 mL	0.3375 mL	0.6749 mL	1.6874 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cinnamic acid621-82-93-Phenylacrylic acid β-Phenylacrylic acidEndogenous MetaboliteInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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营业执照（三证合一）

Cinnamic acid (Synonyms: 肉桂酸; 3-Phenylacrylic acid; β-Phenylacrylic acid)

Customer Review

Other Forms of Cinnamic acid: