2. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor Immunology/Inflammation NF-κB
  3. Endogenous Metabolite LXR Fatty Acid Synthase (FASN) Interleukin Related Reactive Oxygen Species NOD-like Receptor (NLR)
  4. Desmosterol

Desmosterol  (Synonyms: 胆固醇EP杂质B (Desmosterol))

目录号: HY-113224 纯度: ≥98.0%
COA 产品使用指南 技术支持

Desmosterol 是一种类似胆固醇的分子。在胆固醇生物合成的 Bloch 途径中，Desmosterol 是胆固醇的直接前体。Desmosterol 作为内源性代谢物，用于研究胆固醇代谢。Desmosterol 是一种 LXR 激活剂和 SREBP 抑制剂，可抑制巨噬细胞炎症小体活化并防止血管炎症和动脉粥样硬化。Desmosterol 减少会促进巨噬细胞线粒体活性氧 (ROS) 的产生和 NLRP3 的 pyrin 结构域依赖性炎症小体激活。Desmosterol 有望用于炎症、代谢、心血管疾病的研究。

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Desmosterol Chemical Structure

Desmosterol Chemical Structure

CAS No. : 313-04-2

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规格 价格 是否有货 数量
10 mM * 1 mL in DMF ￥2090
In-stock
5 mg ￥1900
In-stock
10 mg ￥3000
In-stock
25 mg ￥5625
In-stock
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Customer Review

Other Forms of Desmosterol:

Desmosterol 相关抗体

Top Publications Citing Use of Products

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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NLRP3 NLRP1 NOD1 NOD2

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Desmosterol is a cholesterol-like molecule. In the Bloch pathway of cholesterol biosynthesis, Desmosterol is a direct precursor of cholesterol. As an endogenous metabolite, Desmosterol is used to study cholesterol metabolism [1]. Desmosterol is an LXR activator and SREBP inhibitor, which can suppress macrophage inflammasome activation and prevent vascular inflammation and atherosclerosis. A reduction in Desmosterol promotes the production of mitochondrial reactive oxygen species (ROS) in macrophages and pyrin domain-dependent inflammasome activation of NLRP3. Desmosterol holds potential for research in inflammation, metabolism, and cardiovascular diseases [1][2][3].

IC50 & Target[1]

Human Endogenous Metabolite

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 ED50
> 50 μg/mL
Compound: 1, desmosterol
Cytotoxicity against human A549 cells by MTT assay
Cytotoxicity against human A549 cells by MTT assay
[PMID: 8984148]
HT-29 ED50
> 50 μg/mL
Compound: 1, desmosterol
Cytotoxicity against human HT29 cells by MTT assay
Cytotoxicity against human HT29 cells by MTT assay
[PMID: 8984148]
KB ED50
> 50 μg/mL
Compound: 1, desmosterol
Cytotoxicity against human KB cells by MTT assay
Cytotoxicity against human KB cells by MTT assay
[PMID: 8984148]
P388 ED50
33.53 μg/mL
Compound: 1, desmosterol
Cytotoxicity against mouse P388 cells by MTT assay
Cytotoxicity against mouse P388 cells by MTT assay
[PMID: 8984148]
体外研究
(In Vitro)

Desmosterol (10 μM, 48 小时) 可在巨噬细胞中激活 LXR 目标蛋白 (Abca1) 表达并抑制 SREBP 目标蛋白 (DHCR24) 表达[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Desmosterol 相关抗体:

Western Blot Analysis[3]

Cell Line: Mouse peritoneal macrophages (TGEM) and human hepatocyte cell line (HepG2)
Concentration: 10 μM
Incubation Time: 48 hours
Result: Activated Abca1 protein expression in TGEMs but had no significant effect in HepG2 cells.

Real Time qPCR[3]

Cell Line: Mouse peritoneal macrophages (TGEM)
Concentration: 10 μM
Incubation Time: 48 h
Result: Suppressed the expression of the SREBP target gene Dhcr24.
体内研究
(In Vivo)

Desmosterol 缺失 (通过腹腔注射 Adenovirus (AAV-Cre)，在 Dhcr24TG 小鼠中激活 DHCR24 基因，来降低巨噬细胞中 Desmosterol 水平) 会在 Dhcr24TG 小鼠体内抑制 LXR 激活，上调免疫反应基因，抑制胆固醇外排能力从而导致巨噬细胞内脂质积累和泡沫细胞形成，与动脉粥样硬化疾病发生紧密相关[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Dhcr24TG and WT mice
Note:
1. Dhcr24TG mice: Crossing Dhcr24fl/fl mice with LysM-Cre mice to obtain conditional Dhcr24TG mice, ensuring that DHCR24 was overexpressed only in macrophages
2. DHCR24 catalyzed the final step in the conversion of Desmosterol to cholesterol. Activating the DHCR24 gene decreased the level of Desmosterol.
Dosage: This experiment did not involve drug administration but altered Desmosterol levels through genetic engineering:
1. Adenovirus (AAV-Cre) was injected (i.p.) to activate DHCR24 in Dhcr24fl/fl mice.
2. After genetic modification, macrophages were isolated from blood and analyzed via Western Blot after 2 weeks of AAV-Cre injection.
Administration: Samples of macrophages were collected starting from 2 weeks after the virus injection, with the total experimental period lasting 4 weeks.
Result: Significantly reduced the LXR signaling in Dhcr24TG mice, and downregulated LXR-dependent genes like ABCA1 and ABCG1 (Desmosterol depletion).
Increased expression of inflammatory genes like Cxcl9, Cxcl10, Il6 in Dhcr24TG macrophages.
分子量

384.64

Formula

C27H44O
CAS 号
性状

固体

颜色

White to off-white

中文名称

胆固醇EP杂质B (Desmosterol)
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验： 

DMF 中的溶解度 : 50 mg/mL (129.99 mM; 超声助溶)

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5998 mL 12.9992 mL 25.9983 mL
5 mM 0.5200 mL 2.5998 mL 5.1997 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: ≥98.0%

COA (286 KB) HNMR (266 KB)

产品使用指南 (1538 KB)
参考文献

Desmosterol 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMF 1 mM 2.5998 mL 12.9992 mL 25.9983 mL 64.9958 mL
5 mM 0.5200 mL 2.5998 mL 5.1997 mL 12.9992 mL
10 mM 0.2600 mL 1.2999 mL 2.5998 mL 6.4996 mL
15 mM 0.1733 mL 0.8666 mL 1.7332 mL 4.3331 mL
20 mM 0.1300 mL 0.6500 mL 1.2999 mL 3.2498 mL
25 mM 0.1040 mL 0.5200 mL 1.0399 mL 2.5998 mL
30 mM 0.0867 mL 0.4333 mL 0.8666 mL 2.1665 mL
40 mM 0.0650 mL 0.3250 mL 0.6500 mL 1.6249 mL
50 mM 0.0520 mL 0.2600 mL 0.5200 mL 1.2999 mL
60 mM 0.0433 mL 0.2167 mL 0.4333 mL 1.0833 mL
80 mM 0.0325 mL 0.1625 mL 0.3250 mL 0.8124 mL
100 mM 0.0260 mL 0.1300 mL 0.2600 mL 0.6500 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Desmosterol313-04-2胆固醇EP杂质B (Desmosterol)Endogenous MetaboliteLXRFatty Acid Synthase (FASN)Interleukin RelatedReactive Oxygen SpeciesNOD-like Receptor (NLR)Liver X receptorILmoleculesimilarendogenousmetabolitecholesterolBlochInhibitorinhibitorinhibit

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