Nifurtimox (Synonyms: 硝呋替莫)

目录号: HY-W040073 纯度: 99.65%: FAQs
Data Sheet SDS COA 产品使用指南技术支持

Nifurtimox 是一种用于锥虫病 (Trypanosoma cruzi) 的抗虫剂。Nifurtimox 有潜力用于神经母细胞瘤细胞的研究。Nifurtimox 影响乳酸脱氢酶 (LDH) 活性。

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Nifurtimox Chemical Structure

CAS No. : 23256-30-6

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10 mM * 1 mL in DMSO	￥600	In-stock
5 mg	￥546	In-stock
10 mg	￥875	In-stock
25 mg	￥1750	In-stock
50 mg	￥2800	In-stock
100 mg	￥3990	In-stock
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Customer Review

Other Forms of Nifurtimox:

Nifurtimox-d₄ 询价
Nifurtimox (Standard) 询价

Nifurtimox 相关产品

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Nifurtimox, an antiprotozoal agent, which is generally used for the treatment of infections with Trypanosoma cruzi, has been used in the therapy of neuroblastoma. Nifurtimox affects enzyme activity of lactate dehydrogenase (LDH).

IC₅₀ & Target

Trypanosoma cruzi^[1]
Lactate dehydrogenase (LDH) ^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
CHO	GI₅₀	13.9 μg/mL Compound: nifurtimox	Cytotoxicity against CHO cells assessed as cell viability after 48 hrs by MTT colorimetric assay Cytotoxicity against CHO cells assessed as cell viability after 48 hrs by MTT colorimetric assay	[PMID: 22551062]
Fibroblast	IC₅₀	> 200 μM Compound: NFX	Cytotoxicity against human fibroblasts assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human fibroblasts assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 23644203]
H9c2	IC₅₀	> 50 μM Compound: Nifurtimox	Cytotoxicity against rat H9c2 cells infected with Trypanosoma cruzi Tulahuen amastigote forms assessed as reduction in cell number after 72 hrs by DRAQ5 DNA dye based confocal microscopic analysis Cytotoxicity against rat H9c2 cells infected with Trypanosoma cruzi Tulahuen amastigote forms assessed as reduction in cell number after 72 hrs by DRAQ5 DNA dye based confocal microscopic analysis	[PMID: 30100019]
HEK293	EC₅₀	> 100 μM Compound: Nifurtimox	Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay	[PMID: 27720295]
HeLa	IC₅₀	12 μM Compound: Nfx	Cytotoxicity against human HeLa cells after 24 hrs by MTT assay Cytotoxicity against human HeLa cells after 24 hrs by MTT assay	[PMID: 19168363]
HeLa	EC₅₀	272 μM Compound: Nfx	Cytotoxicity against human HeLa cells measured after 24 hrs by crystal violet staining based assay Cytotoxicity against human HeLa cells measured after 24 hrs by crystal violet staining based assay	[PMID: 27810595]
HeLa	EC₅₀	91.2 μM Compound: 5	Cytotoxicity against human HeLa cells after 65 hrs by Alamar blue assay Cytotoxicity against human HeLa cells after 65 hrs by Alamar blue assay	[PMID: 23281892]
HepG2	CC₅₀	45.2 μM Compound: Nifurtimox	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32795774]
HepG2	CC₅₀	45.2 μM Compound: Nifurtimox	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32652409]
IMR-90	EC₅₀	> 25 μM Compound: Nifurtimox	Cytotoxicity against human IMR90 cells after 44 to 46 hrs by CellTiter-Blue assay Cytotoxicity against human IMR90 cells after 44 to 46 hrs by CellTiter-Blue assay	[PMID: 28119024]
J774	IC₅₀	> 200 μM Compound: S5	Cytotoxicity against J774.1 cell line after 48 hrs Cytotoxicity against J774.1 cell line after 48 hrs	[PMID: 16516467]
J774	CC₅₀	131.5 μM Compound: Nfx	Cytotoxicity against mouse J774 cells incubated for 24 hrs by resazurin dye based fluorescence assay Cytotoxicity against mouse J774 cells incubated for 24 hrs by resazurin dye based fluorescence assay	[PMID: 28648464]
J774	CC₅₀	131.503 μM Compound: NFX	Cytotoxicity against mouse J774 cells assessed as decrease in cell viability after 24 hrs by resazurin assay Cytotoxicity against mouse J774 cells assessed as decrease in cell viability after 24 hrs by resazurin assay	[PMID: 27503677]
J774	EC₅₀	150 μM Compound: Nfx	Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 24 hrs by WST-1 assay Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 24 hrs by WST-1 assay	[PMID: 31673311]
J774	IC₅₀	280.48 μM Compound: NFX	Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay	[PMID: 23816040]
J774	IC₅₀	316 μM Compound: Nifurtimox	Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28499168]
J774	IC₅₀	316 μM Compound: Nfx	Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 25008454]
J774	IC₅₀	316 μM Compound: Nfx	Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 23811257]
J774.2	CC₅₀	164.2 μM Compound: Nfx	Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay	[PMID: 34571489]
J774.A1	CC₅₀	201.1 μM Compound: Nfx	Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 20 hrs by MTT assay Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 20 hrs by MTT assay	[PMID: 30784876]
L6	IC₅₀	32 μM Compound: Nifurtimox	Cytotoxicity against rat L6 cells after 6 days by resazurin based fluorescence assay Cytotoxicity against rat L6 cells after 6 days by resazurin based fluorescence assay	[PMID: 27591008]
L6	IC₅₀	32 μM Compound: nifurtimox	Cytotoxicity against rat L6 cells after 6 days by resazurin staining based fluorescence plate reader assay Cytotoxicity against rat L6 cells after 6 days by resazurin staining based fluorescence plate reader assay	[PMID: 26479031]
L6	IC₅₀	68 μM Compound: nifurtimox	Concentration causing cytotoxicity to 50% of L-6 rat skeletal myoblasts Concentration causing cytotoxicity to 50% of L-6 rat skeletal myoblasts	[PMID: 16107157]
L6	CC₅₀	78.2 μM Compound: Nifurtimox	Cytotoxicity against rat L6 cells after 5 days by resazurin assay Cytotoxicity against rat L6 cells after 5 days by resazurin assay	[PMID: 25089808]
L929	IC₅₀	> 256 μM Compound: Nifurtimox	Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability after 48 hrs by resazurin dye-based assay Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability after 48 hrs by resazurin dye-based assay	[PMID: 28499168]
LLC-MK2	IC₅₀	1.9 μM Compound: Nfx	Antitrypanosomal activity against epimastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 11 days Antitrypanosomal activity against epimastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 11 days	[PMID: 23167554]
LLC-MK2	CC₅₀	2.7 μM Compound: Nfx	Antitrypanosomal activity against Trypanosoma cruzi Y metacyclic trypomastigotes isolated from infected LLC-MK2 cells assessed as parasite viability after 24 hrs by neubauer chamber analysis Antitrypanosomal activity against Trypanosoma cruzi Y metacyclic trypomastigotes isolated from infected LLC-MK2 cells assessed as parasite viability after 24 hrs by neubauer chamber analysis	[PMID: 24561675]
LLC-MK2	IC₅₀	2.7 μM Compound: Nfx	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 24 hrs Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 24 hrs	[PMID: 23167554]
MRC5	IC₅₀	0.7 μM Compound: Nifurtimox	Trypanocidal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 infected in human MRC5 SV2 cells assessed as parasite growth inhibition after 168 hrs by beta-galactosidase assay Trypanocidal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 infected in human MRC5 SV2 cells assessed as parasite growth inhibition after 168 hrs by beta-galactosidase assay	[PMID: 25199582]
NIH3T3	IC₅₀	3 μg/mL Compound: nifurtimox	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen infected in mouse 3T3 cells assessed as beta-galactosidase activity after 7 days by chagas bioassay Antitrypanosomal activity against Trypanosoma cruzi Tulahuen infected in mouse 3T3 cells assessed as beta-galactosidase activity after 7 days by chagas bioassay	[PMID: 20441198]
RAW264.7	IC₅₀	263.44 μM Compound: Nifurtimox	Cytotoxicity against mouse RAW264.7 cells measured after 4 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells measured after 4 hrs by MTT assay	[PMID: 27908757]
THP-1	IC₅₀	> 100 μM Compound: Nifurtimox	Cytotoxicity against human THP1 cells by AlamarBlue assay Cytotoxicity against human THP1 cells by AlamarBlue assay	[PMID: 24119553]
THP-1	IC₅₀	64.8 μM Compound: nifurtimox	Cytotoxicity against human THP1 cells after 6 days by Alamar blue assay Cytotoxicity against human THP1 cells after 6 days by Alamar blue assay	[PMID: 20028822]
U2OS	EC₅₀	0.34 μM Compound: Nifurtimox	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Y infected in human U2OS cells after 96 hrs by Draq5 staining-based high content screening assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Y infected in human U2OS cells after 96 hrs by Draq5 staining-based high content screening assay	[PMID: 27318979]
U2OS	EC₅₀	1.26 μM Compound: Nifurtimox	Antiparasitic activity against Trypanosoma cruzi Y trypomastigotes infected in rhesus monkey LLC-MK2 cells followed by re-infection of amastigotes in human U2OS cells assessed as reduction in parasite infection level after 96 hrs by draq5 staining based a Antiparasitic activity against Trypanosoma cruzi Y trypomastigotes infected in rhesus monkey LLC-MK2 cells followed by re-infection of amastigotes in human U2OS cells assessed as reduction in parasite infection level after 96 hrs by draq5 staining based a	[PMID: 26774924]
U2OS	CC₅₀	26.8 μM Compound: Nifurtimox	Cytotoxicity against human U2OS cells infected with amastigote stage of Trypanosoma cruzi Y assessed as decrease in number of cells after 96 hrs by Draq5 staining-based high content screening assay Cytotoxicity against human U2OS cells infected with amastigote stage of Trypanosoma cruzi Y assessed as decrease in number of cells after 96 hrs by Draq5 staining-based high content screening assay	[PMID: 27318979]
V79	IC₅₀	35 μM Compound: Nifurtimox	Cytotoxicity against chinese hamster V79 cells after 6 days by Alamar blue assay Cytotoxicity against chinese hamster V79 cells after 6 days by Alamar blue assay	[PMID: 20679506]
Vero	IC₅₀	0.24 μM Compound: Nifurtimox	Antitrypanosomal activity against Trypanosoma cruzi clone Cl-Brener infected in african green monkey Vero cells assessed as growth inhibition after 3 days by luciferase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi clone Cl-Brener infected in african green monkey Vero cells assessed as growth inhibition after 3 days by luciferase reporter gene assay	[PMID: 20679506]
Vero	IC₅₀	0.45 μM Compound: Nfx	Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay	[PMID: 24749923]
Vero	IC₅₀	0.52 μM Compound: Nifurtimox	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 in african green monkey Vero cells after 120 hrs Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 in african green monkey Vero cells after 120 hrs	[PMID: 18798609]
Vero	IC₅₀	1.4 μM Compound: Nfx	Antiparasitic activity against Trypanosoma cruzi 320I04 intracellular amastigotes infected in african green monkey Vero cells after 72 hrs Antiparasitic activity against Trypanosoma cruzi 320I04 intracellular amastigotes infected in african green monkey Vero cells after 72 hrs	[PMID: 19321339]
Vero	IC₅₀	1.6 μM Compound: Nfx	Antiparasitic activity against amastigote stage of Trypanosoma cruzi infected in Vero cells assessed as parasite growth inhibition after 24 hrs by hemocytometery Antiparasitic activity against amastigote stage of Trypanosoma cruzi infected in Vero cells assessed as parasite growth inhibition after 24 hrs by hemocytometery	[PMID: 25173828]
Vero	IC₅₀	10 μM Compound: Nifurtimox	Antitrypanosomal activity against trypomastigote stage of Trypanosoma cruzi Dm28c infected in African green monkey Vero cells assessed as growth inhibition incubated for 5 to 7 days post infection measured after 24 hrs by MTT assay Antitrypanosomal activity against trypomastigote stage of Trypanosoma cruzi Dm28c infected in African green monkey Vero cells assessed as growth inhibition incubated for 5 to 7 days post infection measured after 24 hrs by MTT assay	[PMID: 27908757]
Vero	IC₅₀	10 μM Compound: nifurtimox	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 in vero cells assessed as intracellular growth inhibition of trypomastigote by beta-galactosidase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 in vero cells assessed as intracellular growth inhibition of trypomastigote by beta-galactosidase reporter gene assay	[PMID: 18715036]
Vero	IC₅₀	10.4 μM Compound: Nfx	Trypanosomicidal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 transfected with beta-D-galactosidase infected in african green monkey Vero cells assessed as growth inhibition incubated 5 days prior to beta-D-galactopyranoside addition Trypanosomicidal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 transfected with beta-D-galactosidase infected in african green monkey Vero cells assessed as growth inhibition incubated 5 days prior to beta-D-galactopyranoside addition	[PMID: 23202852]
Vero	IC₅₀	11 μM Compound: Nifurtimox	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 infected in african green monkey Vero cells assessed as growth inhibition of trypomastigotes by beta-galactosidase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 infected in african green monkey Vero cells assessed as growth inhibition of trypomastigotes by beta-galactosidase reporter gene assay	[PMID: 17067146]
Vero	CC₅₀	113.6 μM Compound: Nfx	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19321339]
Vero	IC₅₀	115 μM Compound: NFX	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 30344907]
Vero	IC₅₀	13.5 μM Compound: Nifurtimox	Antiparasitic activity against Trypanosoma cruzi Tulahuen C4 infected african green monkey Vero cells expressing beta-galctosidase after 5 days Antiparasitic activity against Trypanosoma cruzi Tulahuen C4 infected african green monkey Vero cells expressing beta-galctosidase after 5 days	[PMID: 20030365]
Vero	CC₅₀	163 μM Compound: Nfx, Lampit	Cytotoxicity against African green monkey Vero cells after 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 24 hrs by MTT assay	[PMID: 25899334]
Vero	IC₅₀	2.3 μM Compound: Nfx	Antitrypanosomal activity against Trypanosoma cruzi 320I01 amastigotes infected in african green monkey Vero cells after 5 days by Giemsa staining Antitrypanosomal activity against Trypanosoma cruzi 320I01 amastigotes infected in african green monkey Vero cells after 5 days by Giemsa staining	[PMID: 20627590]
Vero	IC₅₀	2.4 μM Compound: Nfx	Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay	[PMID: 24749923]
Vero	IC₅₀	21.05 μM Compound: Nifurtimox	Trypanocidal activity against epimastigote stage of Trypanosoma cruzi infected in african green monkey Vero cells assessed as cell viability after 24 hrs by MTT assay Trypanocidal activity against epimastigote stage of Trypanosoma cruzi infected in african green monkey Vero cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 25127463]
Vero	CC₅₀	32 μM Compound: Nifurtimox	Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay	[PMID: 28645659]
Vero	IC₅₀	4 μM Compound: Nfx	Antiparasitic activity against epimastigote stage of Trypanosoma cruzi infected in Vero cells assessed as parasite growth inhibition after 24 hrs by hemocytometery Antiparasitic activity against epimastigote stage of Trypanosoma cruzi infected in Vero cells assessed as parasite growth inhibition after 24 hrs by hemocytometery	[PMID: 25173828]
Vero	IC₅₀	4.5 μM Compound: Nifurtimox	Antitrypanosomal activity against Trypanosoma cruzi tulahuen C4 trypomastigotes infected in african green monkey Vero cells assessed as CPRG cleavage after 120 hrs by microplate reader Antitrypanosomal activity against Trypanosoma cruzi tulahuen C4 trypomastigotes infected in african green monkey Vero cells assessed as CPRG cleavage after 120 hrs by microplate reader	[PMID: 20356752]
Vero	IC₅₀	4.8 μM Compound: Nfx	Antiparasitic activity against Trypanosoma cruzi 320I04 epimastigotes infected in african green monkey Vero cells after 72 hrs Antiparasitic activity against Trypanosoma cruzi 320I04 epimastigotes infected in african green monkey Vero cells after 72 hrs	[PMID: 19321339]
Vero	IC₅₀	411.13 μM Compound: Nifurtimox	Cytotoxicity against african green monkey Vero cells by MTT assay Cytotoxicity against african green monkey Vero cells by MTT assay	[PMID: 25127463]
Vero	CC₅₀	61.42 μM Compound: Nfx	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 20627590]
Vero	IC₅₀	64.11 μM Compound: Nifurtimox	Cytotoxicity against african green monkey Vero cells after 6 days by Alamar blue assay Cytotoxicity against african green monkey Vero cells after 6 days by Alamar blue assay	[PMID: 20679506]
Vero	IC₅₀	80.1 μM Compound: Nifurtimox	Cytotoxicity against african green monkey Vero cells by MTT assay Cytotoxicity against african green monkey Vero cells by MTT assay	[PMID: 18798609]
Vero	IC₅₀	94 μM Compound: Nifurtimox	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS assay	[PMID: 20356752]

体外研究
(In Vitro)

Nifurtimox affects enzyme activity of lactate dehydrogenase (LDH). To differentiate if this effect is a result of a reduced LDH activity or a shift in pyruvate metabolism due to activation of PDH, the enzyme activity of LDH is determined after 4 h treatment with 50 µg/mL Nifurtimox. Compared to the untreated control, the LDH activity is significantly reduced for LA-N-1 (P=0.005), IMR-32 (P=0.009), LS (P=0.0035) and SK-N-SH (P=0.0065). Nifurtimox reduces cell viability and induces cell cycle arrest and apoptosis in neuroblastoma cells. To characterize the cytotoxic impacts of Nifurtimox on neuroblastoma, 4 cell lines are subjected to several experiments. Cell viability is reduced for all 4 neuroblastoma cell lines after 24 h incubation with 50 µg/mL to an average of 66%, 63%, 62% and 75% (LA-N-1, IMR-32 LS and SK-N-SH, respectively). The reduction is significant compared to the untreated control (P<0.01) and the vehicle control with DMSO (P<0.05) for all cell lines^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

287.30

Formula

C₁₀H₁₃N₃O₅S

CAS 号

23256-30-6

性状

固体

颜色

Light yellow to yellow

中文名称

硝呋替莫

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 150 mg/mL (522.10 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.4807 mL	17.4034 mL	34.8068 mL
5 mM	0.6961 mL	3.4807 mL	6.9614 mL
10 mM	0.3481 mL	1.7403 mL	3.4807 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.70 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.65%

选择批次：

Data Sheet (622 KB) SDS (252 KB)

COA (286 KB) LCMS (269 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Cabanillas Stanchi KM, et al. Nifurtimox reduces N-Myc expression and aerobic glycolysis in neuroblastoma. Cancer Biol Ther. 2015;16(9):1353-63. [Content Brief]

Cell Assay ^[1]	The Neuroblastoma cell lines IMR-32, LA-N-1 and SK-N-SH and the neuroblastoma cell line LS are grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum (FCS), 2 mM L-glutamine, 100 U/mL Penicillin and 100 µg/mL Streptomycin and incubated at 37°C, 5% CO₂ and saturated humidity. To assess the cell viability after incubation with Nifurtimox at different concentrations (10 µg/mL up to 50 µg/mL or 34.8 µM to 174 µM, respectively in the supernatant growth medium) or the vehicle control with according concentrations, all neuroblastoma cell lines are subjected to an MTS assay. Stock solutions of MTS are made at 480 µM in sterile filtered deionized water and stored at -20°C. Cells are grown to approximately 50% confluency, treated with Nifurtimox, and incubated for 1 h with fresh media containing 12 µM MTS^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Cabanillas Stanchi KM, et al. Nifurtimox reduces N-Myc expression and aerobic glycolysis in neuroblastoma. Cancer Biol Ther. 2015;16(9):1353-63. [Content Brief]

Cell Assay
^[1]

The Neuroblastoma cell lines IMR-32, LA-N-1 and SK-N-SH and the neuroblastoma cell line LS are grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum (FCS), 2 mM L-glutamine, 100 U/mL Penicillin and 100 µg/mL Streptomycin and incubated at 37°C, 5% CO₂ and saturated humidity. To assess the cell viability after incubation with Nifurtimox at different concentrations (10 µg/mL up to 50 µg/mL or 34.8 µM to 174 µM, respectively in the supernatant growth medium) or the vehicle control with according concentrations, all neuroblastoma cell lines are subjected to an MTS assay. Stock solutions of MTS are made at 480 µM in sterile filtered deionized water and stored at -20°C. Cells are grown to approximately 50% confluency, treated with Nifurtimox, and incubated for 1 h with fresh media containing 12 µM MTS^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Cabanillas Stanchi KM, et al. Nifurtimox reduces N-Myc expression and aerobic glycolysis in neuroblastoma. Cancer Biol Ther. 2015;16(9):1353-63. [Content Brief]

Nifurtimox 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4807 mL	17.4034 mL	34.8068 mL	87.0171 mL
	5 mM	0.6961 mL	3.4807 mL	6.9614 mL	17.4034 mL
	10 mM	0.3481 mL	1.7403 mL	3.4807 mL	8.7017 mL
	15 mM	0.2320 mL	1.1602 mL	2.3205 mL	5.8011 mL
	20 mM	0.1740 mL	0.8702 mL	1.7403 mL	4.3509 mL
	25 mM	0.1392 mL	0.6961 mL	1.3923 mL	3.4807 mL
	30 mM	0.1160 mL	0.5801 mL	1.1602 mL	2.9006 mL
	40 mM	0.0870 mL	0.4351 mL	0.8702 mL	2.1754 mL
	50 mM	0.0696 mL	0.3481 mL	0.6961 mL	1.7403 mL
	60 mM	0.0580 mL	0.2901 mL	0.5801 mL	1.4503 mL
	80 mM	0.0435 mL	0.2175 mL	0.4351 mL	1.0877 mL
	100 mM	0.0348 mL	0.1740 mL	0.3481 mL	0.8702 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nifurtimox23256-30-6硝呋替莫ParasiteLactate DehydrogenaseLDHInhibitorinhibitorinhibit

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摩尔计算器

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