2. Anti-infection Cell Cycle/DNA Damage
  3. Bacterial Antibiotic DNA/RNA Synthesis
  4. Rifaximin

Rifaximin  (Synonyms: 利福昔明)

目录号: HY-13234 纯度: 99.88%
COA 产品使用指南

摩尔计算器

Rifaximin是一种胃肠道选择性的抗生素 (antibiotic)，与细菌 DNA 依赖性 RNA 聚合酶的 β 亚基结合，从而抑制细菌 RNA 的合成 (bacterial RNA synthesis)。与革兰氏阴性菌（MIC：8-50 毫克/毫升）相比，Rifaximin对革兰氏阳性菌株（MIC：0.03-5 毫克/毫升）的敏感性更高。

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Rifaximin Chemical Structure

Rifaximin Chemical Structure

CAS No. : 80621-81-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥572
In-stock
500 mg ￥520
In-stock
1 g ￥836
In-stock
5 g ￥3100
In-stock
10 g   询价  
50 g   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Rifaximin:

Rifaximin 相关抗体

Top Publications Citing Use of Products

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Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Rifaximin, a gastrointestinal-selective antibiotic, binds the β-subunit of bacterial DNA-dependent RNA polymerase, resulting in inhibition of bacterial RNA synthesis. Rifaximin susceptibility is higher against Gram-positive strains (MIC: 0.03-5 mg/ml) compared to Gram-negative bacteria (MIC: 8-50 mg/mL)[1][2].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
HepG2 EC50
11.2 μM
Compound: Rifaximin
Transactivation of full length human PXR transfected in human HepG2 cells after 18 hrs by luciferase reporter assay relative to rifaximin
Transactivation of full length human PXR transfected in human HepG2 cells after 18 hrs by luciferase reporter assay relative to rifaximin
[PMID: 26408814]
HepG2 EC50
14.1 μM
Compound: Rifaximin
Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis
Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis
[PMID: 20966043]
HepG2 EC50
14.1 μM
Compound: Rifaximin
Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis
Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis
[PMID: 20966043]
HepG2 EC50
2.2 μM
Compound: rifaximin
Transactivation of human PXR expressed in HepG2 cells after 18 hrs by luciferase reporter gene assay
Transactivation of human PXR expressed in HepG2 cells after 18 hrs by luciferase reporter gene assay
[PMID: 21599020]
HepG2 EC50
4 μM
Compound: rifaximin
Agonist activity at human full-length PXR transfected in human HepG2 cells co-transfected with pSG5-RXR assessed as induction of transactivation by dual-luciferase reporter gene assay
Agonist activity at human full-length PXR transfected in human HepG2 cells co-transfected with pSG5-RXR assessed as induction of transactivation by dual-luciferase reporter gene assay
[PMID: 24388834]
体外研究
(In Vitro)

Rifaximin has a good inhibitory activity against Staphylococcus, Streptococcus, Enterococcus, Escherichia coli, Shigella, Salmonella, Bacillus cereus, Moraxella catarrhalis, Haemophilus influenzae, Haemophilus ducreyi, Bacteroides bivius-disiens, Gardnerella vaginalis, Lactobacillus spp.,Mobiluncus spp.,Neisseria gonorrhoeae, Pseudomonas and Acinetobacter . Rifaximin rarely causes side effects[1].
Rifaximin (0.1, 1.0 and 10.0 µM) causes significant and concentration-dependent reduction of cell proliferation, cell migration and PCNA expression in the Caco-2 cells vs. untreated cells[2].
Rifaximin (0.1-10 μM) downregulates Akt/mTOR and p38MAPK/NF-κB pathways through a PXR-dependent mechanism[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Rifaximin 相关抗体:

Cell Viability Assay[2]

Cell Line: Caco-2 cells
Concentration: 0.1, 1.0 and 10.0 μM
Incubation Time: 48 hours
Result: Caused a significant and concentration-dependent reduction in cell proliferation. Reduced the expression of PCNA in a concentration-dependent manner.

Western Blot Analysis[2]

Cell Line: Caco-2 cells
Concentration: 0.1, 1.0 and 10.0 μM
Incubation Time: 24 hours
Result: Reduced Akt, mTOR, p38 MAPK and HIF-1α expression in a concentration-dependent manner. Inhibited NF-κB nuclear activation and p70S6K.
体内研究
(In Vivo)

Rifaximin administration (30 or 50 mg/kg/day) increases survival rates of colitic mice and reduces colitis severity by improvement of wasting syndrome, histologic scores, decrease in colon IL-2, IL-12, IFN-gamma and TNF-alpha (protein and mRNA) levels, and diminishes colon myeloperoxidase (MPO) activity[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c mice (6–8 weeks old) bearing 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis[3]
Dosage: 10, 30 and 50 mg/kg/day
Administration: Orally, p.o. daily for 7 days
Result: Significantly reduced TNBS induced colitis at the dose of 30 and 50 mg/kg, but not 10 mg/kg. A 7-day course of 30 and 50 mg/kg resulted in an almost complete tissue protection.
Clinical Trial
分子量

785.88

Formula

C43H51N3O11
CAS 号
性状

固体

颜色

Yellow to orange

中文名称

利福昔明
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : ≥ 50 mg/mL (63.62 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2725 mL 6.3623 mL 12.7246 mL
5 mM 0.2545 mL 1.2725 mL 2.5449 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3 mg/mL (3.82 mM); 澄清溶液

    此方案可获得 ≥ 3 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 30.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% Corn Oil

    Solubility: ≥ 3 mg/mL (3.82 mM); 澄清溶液

    此方案可获得 ≥ 3 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。

    以 1 mL 工作液为例，取 100 μL 30.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.88%

COA (201 KB) HNMR (300 KB) LCMS (268 KB)

产品使用指南 (1538 KB)
参考文献

Rifaximin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2725 mL 6.3623 mL 12.7246 mL 31.8115 mL
5 mM 0.2545 mL 1.2725 mL 2.5449 mL 6.3623 mL
10 mM 0.1272 mL 0.6362 mL 1.2725 mL 3.1811 mL
15 mM 0.0848 mL 0.4242 mL 0.8483 mL 2.1208 mL
20 mM 0.0636 mL 0.3181 mL 0.6362 mL 1.5906 mL
25 mM 0.0509 mL 0.2545 mL 0.5090 mL 1.2725 mL
30 mM 0.0424 mL 0.2121 mL 0.4242 mL 1.0604 mL
40 mM 0.0318 mL 0.1591 mL 0.3181 mL 0.7953 mL
50 mM 0.0254 mL 0.1272 mL 0.2545 mL 0.6362 mL
60 mM 0.0212 mL 0.1060 mL 0.2121 mL 0.5302 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rifaximin80621-81-4利福昔明BacterialAntibioticDNA/RNA SynthesisantibioticgastrointestinalbacterialinfectiondiarrheaInhibitorinhibitorinhibit

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