Rolipram (Synonyms: 咯利普兰; (R,S)-Rolipram; (±)-Rolipram; ZK 62711)

目录号: HY-16900 纯度: 99.47%: FAQs
Data Sheet SDS COA 产品使用指南技术支持

Rolipram 是一种选择性的磷酸二酯酶 PDE4 抑制剂，抑制 PDE4A，PDE4B 和 PDE4D，的 IC₅₀ 分别为 3 nM，130 nM 和 240 nM。

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Rolipram Chemical Structure

CAS No. : 61413-54-5

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥550	In-stock
1 mg	￥156	In-stock
5 mg	￥312	In-stock
10 mg	￥500	In-stock
50 mg	￥700	In-stock
100 mg	￥980	In-stock
200 mg		询价
500 mg		询价

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Customer Review

Other Forms of Rolipram:

MCE 顾客使用本产品发表的 14 篇科研文献

Rolipram 相关产品

•相关化合物库:

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•同靶点蛋白产品:

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Rolipram is a selective phosphodiesterases PDE4 inhibitor with IC₅₀s of 3 nM, 130 nM and 240 nM for PDE4A, PDE4B, and PDE4D, respectively.

IC₅₀ & Target

PDE4

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: Rol	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 34311158]
HEK293	EC₅₀	0.16 μM Compound: Rolipram	Inhibition of PDE4B1 (unknown origin) transfected in HEK293 cells assessed as forskolin-induced cAMP accumulation pretreated for 30 mins before forskolin challenge by luciferase reporter gene assay Inhibition of PDE4B1 (unknown origin) transfected in HEK293 cells assessed as forskolin-induced cAMP accumulation pretreated for 30 mins before forskolin challenge by luciferase reporter gene assay	10.1039/C2MD00273F
HEK293	EC₅₀	0.22 μM Compound: Rolipram	Inhibition of PDE4B expressed in HEK293 cells assessed as fold reduction in forskolin-stimulated cAMP production pretreated for 30 mins measured 4 hrs after forskolin challenge by luciferase reporter gene assay Inhibition of PDE4B expressed in HEK293 cells assessed as fold reduction in forskolin-stimulated cAMP production pretreated for 30 mins measured 4 hrs after forskolin challenge by luciferase reporter gene assay	[PMID: 22386978]
HEK293	EC₅₀	0.67 μM Compound: Rolipram	Inhibition of PDE4D3 (unknown origin) transfected in HEK293 cells assessed as forskolin-induced cAMP accumulation pretreated for 30 mins before forskolin challenge by luciferase reporter gene assay Inhibition of PDE4D3 (unknown origin) transfected in HEK293 cells assessed as forskolin-induced cAMP accumulation pretreated for 30 mins before forskolin challenge by luciferase reporter gene assay	10.1039/C2MD00273F
HEK293	EC₅₀	131.5 nM Compound: 1	Inhibition of PDE4 expressed in HEK293 cells coexpressing G-protein coupled receptor and cyclic nucleotide gated ion channel by fluorescence assay Inhibition of PDE4 expressed in HEK293 cells coexpressing G-protein coupled receptor and cyclic nucleotide gated ion channel by fluorescence assay	[PMID: 19464886]
HEK293	EC₅₀	2.62 μM Compound: Rolipram	Inhibition of PDE4A4 (unknown origin) transfected in HEK293 cells assessed as forskolin-induced cAMP accumulation pretreated for 30 mins before forskolin challenge by luciferase reporter gene assay Inhibition of PDE4A4 (unknown origin) transfected in HEK293 cells assessed as forskolin-induced cAMP accumulation pretreated for 30 mins before forskolin challenge by luciferase reporter gene assay	10.1039/C2MD00273F
MCF7	IC₅₀	> 100 μM Compound: Rol	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 34311158]
NCI-H460	IC₅₀	> 100 μM Compound: Rol	Antiproliferative activity against human NCI-H460 cells after 48 hrs by MTT assay Antiproliferative activity against human NCI-H460 cells after 48 hrs by MTT assay	[PMID: 34311158]
PBMC	IC₅₀	> 30 μM Compound: IV	Cytotoxicity against human PBMC by MTS assay Cytotoxicity against human PBMC by MTS assay	[PMID: 29173945]
PBMC	IC₅₀	0.13 μM Compound: Rolipram	Anti-inflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 24 hrs by ELISA Anti-inflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 24 hrs by ELISA	[PMID: 29432948]
PBMC	EC₅₀	0.13 μM Compound: rolipram	Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 24 hrs by ELISA Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha production after 24 hrs by ELISA	[PMID: 22891752]
PBMC	IC₅₀	0.15 μM Compound: Rolipram	Inhibition of LPS stimulated Tumor Necrosis Factor-alpha (TNF-alpha) release by human PBMC Inhibition of LPS stimulated Tumor Necrosis Factor-alpha (TNF-alpha) release by human PBMC	[PMID: 9873600]
PBMC	IC₅₀	0.16 μM Compound: Rolipram	Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha release in after 24 hrs by FACS Array analysis Antiinflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha release in after 24 hrs by FACS Array analysis	[PMID: 19303290]
PBMC	IC₅₀	0.23 μM Compound: Rolipram	Antiinflammatory activity in human PBMC assessed as inhibition of phytohemagglutininin-induced IL2 release after 24 hrs by FACS Array analysis Antiinflammatory activity in human PBMC assessed as inhibition of phytohemagglutininin-induced IL2 release after 24 hrs by FACS Array analysis	[PMID: 19303290]
PBMC	IC₅₀	0.23 μM Compound: Rolipram	Antiinflammatory activity in human PBMC assessed as inhibition of phytohemagglutininin-induced IFN-gamma release after 24 hrs by FACSArray analysis Antiinflammatory activity in human PBMC assessed as inhibition of phytohemagglutininin-induced IFN-gamma release after 24 hrs by FACSArray analysis	[PMID: 19303290]
PBMC	IC₅₀	0.5 μM Compound: Rolipram	Antiinflammatory activity in human PBMC assessed as inhibition of phytohemagglutininin-induced IL5 release after 48 hrs by FACS Array analysis Antiinflammatory activity in human PBMC assessed as inhibition of phytohemagglutininin-induced IL5 release after 48 hrs by FACS Array analysis	[PMID: 19303290]
PBMC	IC₅₀	0.88 μM Compound: Rolipram	Antiinflammatory activity in human PBMC assessed as inhibition of phytohemagglutininin-induced IL10 release after 48 hrs by FACS Array analysis Antiinflammatory activity in human PBMC assessed as inhibition of phytohemagglutininin-induced IL10 release after 48 hrs by FACS Array analysis	[PMID: 19303290]
PBMC	IC₅₀	10.85 μM Compound: 1	Inhibition of LPS-induced TNFalpha production in human PBMC pre-incubated for 30 mins before LPS stimulation for 18 hrs by TR-FRET assay Inhibition of LPS-induced TNFalpha production in human PBMC pre-incubated for 30 mins before LPS stimulation for 18 hrs by TR-FRET assay	[PMID: 28888661]
PBMC	IC₅₀	12.5 μM Compound: Rolipram	Anti-inflammatory activity in human PBMC assessed as suppression of LPS-induced TNF-alpha release preincubated for 30 mins followed by LPS stimulation for 18 hrs by TR-FRET assay Anti-inflammatory activity in human PBMC assessed as suppression of LPS-induced TNF-alpha release preincubated for 30 mins followed by LPS stimulation for 18 hrs by TR-FRET assay	[PMID: 27289320]
PBMC	IC₅₀	18.6 μM Compound: Rolipram	Anti-inflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha secretion preincubated for 30 mins followed by LPS stimulation for 18 hrs by TR-FRET assay Anti-inflammatory activity in human PBMC assessed as inhibition of LPS-induced TNFalpha secretion preincubated for 30 mins followed by LPS stimulation for 18 hrs by TR-FRET assay	[PMID: 28196708]
RAW264.7	IC₅₀	0.19 μM Compound: Rolipram	Inhibition of LPS-induced TNFalpha production in mouse RAW264.7 cells pretreated for 30 mins before LPS challenge by ELISA assay Inhibition of LPS-induced TNFalpha production in mouse RAW264.7 cells pretreated for 30 mins before LPS challenge by ELISA assay	10.1039/C2MD00273F
Sf9	IC₅₀	0.15 μM Compound: 4	Inhibition of human full length N-terminal GST-tagged PDE4B1 expressed in baculovirus infected Sf9 cells using cAMP as substrate incubated for 1 hr by fluorescence polarization assay Inhibition of human full length N-terminal GST-tagged PDE4B1 expressed in baculovirus infected Sf9 cells using cAMP as substrate incubated for 1 hr by fluorescence polarization assay	[PMID: 30822711]
Sf9	IC₅₀	0.3 μM Compound: 4	Inhibition of human N-terminal GST-tagged PDE4D7 (2 to 748 residues) expressed in baculovirus infected Sf9 cells using cAMP as substrate after 1 hr by fluorescence polarization assay Inhibition of human N-terminal GST-tagged PDE4D7 (2 to 748 residues) expressed in baculovirus infected Sf9 cells using cAMP as substrate after 1 hr by fluorescence polarization assay	[PMID: 30822711]
Sf9	IC₅₀	0.94 μM Compound: Rolipram	Inhibition of His-tagged recombinant human PDE4B1 expressed in baculovirus infected sf9 cells using cAMP as substrate preincubated for 15 mins followed by substrate addition measured after 1 hr by luminescence assay Inhibition of His-tagged recombinant human PDE4B1 expressed in baculovirus infected sf9 cells using cAMP as substrate preincubated for 15 mins followed by substrate addition measured after 1 hr by luminescence assay	10.1039/C2MD00273F
Sf9	IC₅₀	0.94 μM Compound: Rolipram	Inhibition of recombinant His-tagged human PDE4B expressed in Sf9 cells using cAMP as substrate preincubated with enzyme for 15 mins followed by substrate addition and incubated for 1 hr by PDELight HTS cAMP phosphodiesterase Kit based luminometry Inhibition of recombinant His-tagged human PDE4B expressed in Sf9 cells using cAMP as substrate preincubated with enzyme for 15 mins followed by substrate addition and incubated for 1 hr by PDELight HTS cAMP phosphodiesterase Kit based luminometry	[PMID: 31035240]
Sf9	IC₅₀	0.941 μM Compound: Rolipram	Inhibition of PDE4B1 (unknown origin) expressed in sf9 cells using cAMP as substrate preincubated for 15 mins before substrate addition measured after 1 hr by luminescence assay Inhibition of PDE4B1 (unknown origin) expressed in sf9 cells using cAMP as substrate preincubated for 15 mins before substrate addition measured after 1 hr by luminescence assay	[PMID: 23415088]
Sf9	IC₅₀	1 μM Compound: Rolipram	Inhibition of recombinant His-tagged PDE4B (unknown origin) expressed in Sf9 cells using cAMP as substrate incubated for 15 mins prior to substrate addition measured after 1 hr by luminescence-based assay Inhibition of recombinant His-tagged PDE4B (unknown origin) expressed in Sf9 cells using cAMP as substrate incubated for 15 mins prior to substrate addition measured after 1 hr by luminescence-based assay	[PMID: 23380174]
Sf9	IC₅₀	1.03 μM Compound: Rolipram	Inhibition of recombinant human PDE4B expressed in baculovirus infected Sf9 cells using [3H] cAMP as substrate after 30 min by scintillation proximity assay Inhibition of recombinant human PDE4B expressed in baculovirus infected Sf9 cells using [3H] cAMP as substrate after 30 min by scintillation proximity assay	[PMID: 32209292]
Sf9	IC₅₀	2.3 μM Compound: Rolipram	Inhibition of human recombinant PDE4 catalytic domain expressed in baculovirus-infected insect Sf9 cells by modified two-step method Inhibition of human recombinant PDE4 catalytic domain expressed in baculovirus-infected insect Sf9 cells by modified two-step method	[PMID: 19303290]
Sf9	IC₅₀	288 nM Compound: CHEMBL63	Inhibition of recombinant human PDE4D7 catalytic domain expressed in baculovirus infected sf9 cells using cAMP as substrate Inhibition of recombinant human PDE4D7 catalytic domain expressed in baculovirus infected sf9 cells using cAMP as substrate	[PMID: 26908025]
T-cell	IC₅₀	> 10 μM Compound: Rolipram	Inhibitory concentration against T cell proliferation using concanavalin A stimulated mice spleen cells Inhibitory concentration against T cell proliferation using concanavalin A stimulated mice spleen cells	[PMID: 11229767]
U-251	IC₅₀	> 100 μM Compound: Rol	Antiproliferative activity against human U-251 cells after 48 hrs by MTT assay Antiproliferative activity against human U-251 cells after 48 hrs by MTT assay	[PMID: 34311158]
U-937	IC₅₀	0.86 μM Compound: Rolipram	Inhibition of PDE4 in human U937 cells assessed as accumulation of [3H]adenosine by scintillation counting Inhibition of PDE4 in human U937 cells assessed as accumulation of [3H]adenosine by scintillation counting	[PMID: 19303290]
U-937	IC₅₀	1.12 μM Compound: Rolipram	Anti-inflammatory activity in human U937 cells assessed as inhibition of LPS-induced TNFalpha production pre-incubated for 1 hr before LPS stimulation for 24 hrs by ELISA method Anti-inflammatory activity in human U937 cells assessed as inhibition of LPS-induced TNFalpha production pre-incubated for 1 hr before LPS stimulation for 24 hrs by ELISA method	[PMID: 28927905]
U-937	IC₅₀	1.17 μM Compound: Rolipram	Anti-inflammatory activity in human U937 cells assessed as inhibition of LPS-induced IL6 production pre-incubated for 1 hr before LPS stimulation for 24 hrs by ELISA method Anti-inflammatory activity in human U937 cells assessed as inhibition of LPS-induced IL6 production pre-incubated for 1 hr before LPS stimulation for 24 hrs by ELISA method	[PMID: 28927905]
U-937	IC₅₀	1.3 μM Compound: 1	Inhibition of PDE4B isolated from human U937 cells using [3H]-cAMP as substrate after 30 mins Inhibition of PDE4B isolated from human U937 cells using [3H]-cAMP as substrate after 30 mins	[PMID: 26320621]
U-937	IC₅₀	880 nM Compound: (+/-)-Rolipram	Inhibition of PDE4 isolated from human U937 cells using [3H]-cAMP as substrate after 30 mins by liquid scintillation counting analysis Inhibition of PDE4 isolated from human U937 cells using [3H]-cAMP as substrate after 30 mins by liquid scintillation counting analysis	10.1039/C0MD00215A

体外研究
(In Vitro)

PDE4 选择性抑制剂 Rolipram 可类似地抑制免疫纯化的 PDE4B 和 PDE4D 活性，其 IC₅₀ 分别为 130 nM和 240 nM。相反，Rolipram 抑制免疫纯化的 PDE4A 活性，IC₅₀ 显著降低，约为 3 nM。Rolipram 以剂量依赖性方式增加 U937 细胞中 cAMP 反应元件结合蛋白 (CREB) 的磷酸化，这意味着存在高亲和力 (IC₅₀ 约 1 nM) 和低亲和力亲和力 (IC₅₀ 约 120 nM) 成分。Rolipram 以简单的单调方式剂量依赖性地抑制 IFN-γ 刺激的 p38 MAPK 磷酸化，IC₅₀ 约为 290 nM^[1]。
Rolipram 是一种选择性 PDE4 抑制剂，可抑制所有 PDE4 亚型 A、B、C 和 D。Rolipram 以剂量依赖性方式 (IC₅₀ 25.9 nM) 和最大/次最大抑制 LPS 诱导的 TNF 产生在 J774 细胞中观察到 2 μM 药物浓度的抑制作用^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

LPS 在 WT 小鼠的腹膜巨噬细胞 (PM) 中诱导 TNF mRNA 和蛋白质表达，这显然被 Rolipram 抑制 (TNF mRNA 和 TNF 蛋白质分别抑制 74% 和 63%)。与 WT 小鼠的 PM 相比，LPS 诱导的 TNF 生成在 MKP-1 (-/-) 小鼠的 PM 中得到增强，这与已发表的结果一致。有趣的是，Rolipram 对 TNF mRNA 和蛋白质表达的抑制在 MKP-1 (-/-) 小鼠的 PM 中显著减弱，并且没有达到统计学显著性^[2]。
重复给予 Rolipram (1.25 mg/kg，ip) 可减少习得性无助大鼠逃脱失败的次数^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

275.34

Formula

C₁₆H₂₁NO₃

CAS 号

61413-54-5

性状

固体

颜色

White to off-white

中文名称

咯利普兰

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 41 mg/mL (148.91 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : < 0.1 mg/mL (insoluble)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6319 mL	18.1594 mL	36.3187 mL
5 mM	0.7264 mL	3.6319 mL	7.2637 mL
10 mM	0.3632 mL	1.8159 mL	3.6319 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.08 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.08 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.08 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.47%

选择批次：

Data Sheet (650 KB) SDS (393 KB)

COA (285 KB) HNMR (285 KB) LCMS (86 KB)

产品使用指南 (1538 KB)

参考文献

[1]. MacKenzie SJ, et al. Action of rolipram on specific PDE4 cAMP phosphodiesterase isoforms and on the phosphorylation of cAMP-response-element-binding protein (CREB) and p38 mitogen-activated protein (MAP) kinase in U937 monocyticcells. Biochem J. 2000 Apr [Content Brief]

[2]. Korhonen R, et al. Attenuation of TNF production and experimentally induced inflammation by PDE4 inhibitor rolipram is mediated by MAPK phosphatase-1. Br J Pharmacol. 2013 Aug;169(7):1525-36. [Content Brief]

[3]. Shalaby AM, et al. Effect of rolipram, a phosphodiesterase enzyme type-4 inhibitor, on γ-amino butyric acid content of the frontal cortex in mice exposed to chronic mild stress. J Pharmacol Pharmacother. 2012 Apr;3(2):132-7. [Content Brief]

Cell Assay ^[2]	J774 murine macrophages (ATCC) are cultured at 37°C in 5% CO₂ atmosphere in DMEM supplemented with glutamax-1 containing 10% heat-inactivated FBS. For experiments, cells are seeded on 24-well plates at a density of 2×10⁵ cells per well. Cell monolayers are grown for 72 h before the experiments are started. Rolipram, IBMX and BIRB 796 are dissolved in DMSO, and 8-Br-cAMP in HBSS. LPS (10 ng/mL) or the compounds of interest at concentrations indicated or the solvent (DMSO, 0.1% v/v) are added to the cells in fresh culture medium containing 10% FBS and the supplements. Cells are further incubated for the time indicated. The effect of LPS and the tested chemicals on cell viability is evaluated by Cell Proliferation Kit II (XTT)^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Mice^[2] Inbred C57BL/6 MKP-1(-/-) mice are used. C57BL/6 mice (20-25 g) are divided into groups of six mice and treated with 200 μL of PBS or Rolipram (100 mg/kg in PBS) by an i.p. injection 2 h before applying carrageenan. Before the administration of carrageenan, the mice are anaesthetized by i.p. injection of 0.5 mg/kg of medetomidine. The mice receive a 30 μL i.d. injection of carrageenan (1.5%, dissolved in normal saline) in one hind paw. The contralateral paw receive 30 μL of saline and it is used as a control. Paw volume is measured before and 3 h after the carrageenan injection with a plethysmometer. Oedema is expressed as a change in paw volume over time. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. MacKenzie SJ, et al. Action of rolipram on specific PDE4 cAMP phosphodiesterase isoforms and on the phosphorylation of cAMP-response-element-binding protein (CREB) and p38 mitogen-activated protein (MAP) kinase in U937 monocyticcells. Biochem J. 2000 Apr [Content Brief] [2]. Korhonen R, et al. Attenuation of TNF production and experimentally induced inflammation by PDE4 inhibitor rolipram is mediated by MAPK phosphatase-1. Br J Pharmacol. 2013 Aug;169(7):1525-36. [Content Brief] [3]. Shalaby AM, et al. Effect of rolipram, a phosphodiesterase enzyme type-4 inhibitor, on γ-amino butyric acid content of the frontal cortex in mice exposed to chronic mild stress. J Pharmacol Pharmacother. 2012 Apr;3(2):132-7. [Content Brief]

Rolipram 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6319 mL	18.1594 mL	36.3187 mL	90.7968 mL
	5 mM	0.7264 mL	3.6319 mL	7.2637 mL	18.1594 mL
	10 mM	0.3632 mL	1.8159 mL	3.6319 mL	9.0797 mL
	15 mM	0.2421 mL	1.2106 mL	2.4212 mL	6.0531 mL
	20 mM	0.1816 mL	0.9080 mL	1.8159 mL	4.5398 mL
	25 mM	0.1453 mL	0.7264 mL	1.4527 mL	3.6319 mL
	30 mM	0.1211 mL	0.6053 mL	1.2106 mL	3.0266 mL
	40 mM	0.0908 mL	0.4540 mL	0.9080 mL	2.2699 mL
	50 mM	0.0726 mL	0.3632 mL	0.7264 mL	1.8159 mL
	60 mM	0.0605 mL	0.3027 mL	0.6053 mL	1.5133 mL
	80 mM	0.0454 mL	0.2270 mL	0.4540 mL	1.1350 mL
	100 mM	0.0363 mL	0.1816 mL	0.3632 mL	0.9080 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Rolipram61413-54-5(R,S)-Rolipram (±)-Rolipram ZK 62711咯利普兰ZK62711ZK 62711ZK-62711Phosphodiesterase (PDE)BacterialHIVHuman immunodeficiency virusInhibitorinhibitorinhibit

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Rolipram (Synonyms: 咯利普兰; (R,S)-Rolipram; (±)-Rolipram; ZK 62711)

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MCE 顾客使用本产品发表的 14 篇科研文献

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Rolipram (Synonyms: 咯利普兰; (R,S)-Rolipram; (±)-Rolipram; ZK 62711)

Customer Review

Other Forms of Rolipram:

Rolipram 相关抗体

MCE 顾客使用本产品发表的 14 篇科研文献

Rolipram 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 Phosphodiesterase (PDE) 亚型特异性产品:

查看 HIV 亚型特异性产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

Rolipram 相关抗体:

摩尔计算器

稀释计算器

Rolipram 相关分类

完整储备液配制表

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