Stattic

目录号: HY-13818 纯度: 99.60%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Stattic 是一种有效的 STAT3 抑制剂，可以抑制 STAT3 磷酸化 (磷酸化位点为 Y705 和 S727)。Stattic 抑制高亲和力磷酸肽与 STAT3 的 SH2 域结合。Stattic 可改善 Alport 综合征 (AS) 小鼠的肾功能不全。

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Stattic Chemical Structure

CAS No. : 19983-44-9

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥550	In-stock
5 mg	￥312	In-stock
10 mg	￥500	In-stock
25 mg	￥800	In-stock
50 mg	￥1300	In-stock
100 mg	￥2000	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

MCE 顾客使用本产品发表的 175 篇科研文献

Proliferation Assay

: Stattic purchased from MCE. Usage Cited in: Mol Med Rep. 2023 Apr;27(4):81. [Abstract]; Stattic (5, 15, 30 µM; 4 h) markedly inhibits Y705 STAT3 phosphorylation in both senescent TC‑1 (Fig. C) and TRAMP‑C2 (Fig. D) cells in a concentration‑dependent manner.

: Stattic purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2022 Nov 11;248:114268. [Abstract]; Stattic (10 μM) effectively attenuates the SCCP-mediated increase in iNOS and GFAP expression in C6 cells.

: Stattic purchased from MCE. Usage Cited in: Mol Cancer. 2019 Mar 30;18(1):64. [Abstract]; Western blot analysis of HCT116 cells alone, IL6-supplemented HCT116, TAMs-co-cultured HCT116 in the presence or absence of Stattic (15 μM).

: Stattic purchased from MCE. Usage Cited in: Cell Signal. 2019 Mar;55:65-72. [Abstract]; Western blotting and densitometric analysis of p-STAT3 in Lo2 cells after 24 h of treatment with the indicated concentrations of the STAT3 inhibitor stattic.

: Stattic purchased from MCE. Usage Cited in: IUBMB Life. 2018 Jan;70(1):81-91. [Abstract]; A549 cells are treated with TG101348 (3 nM), BMS-911543 (1.5 nM), and Stattic (2.5 lM) for 24 h, and the conditioned media are collected and applied for tube formation assay in HUVECs.

: Stattic purchased from MCE. Usage Cited in: Mol Carcinog. 2017 Nov;56(11):2434-2445. [Abstract]; Cells are treated with the STAT3 inhibitor Stattic. The inhibitory action of Stattic on STAT3 phosphorylation is confirmed by Western blot.

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生物活性
纯度 & 产品资料
参考文献

生物活性

Stattic is a potent STAT3 inhibitor and inhibits STAT3 phosphorylation (at Y705 and S727)^[1]. Stattic inhibits the binding of a high affinity phosphopeptide for the SH2 domain of STAT3^[2]. Stattic ameliorates the renal dysfunction in Alport syndrome (AS) mice^[3].

IC₅₀ & Target^[1]^[2]

STAT3

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	2.5 μM Compound: Stattic	Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26396689]
A549	IC₅₀	4.4 μM Compound: Stattic	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 27718470]
ASPC1	IC₅₀	1.32 μM Compound: Stattic	Antiproliferative activity against human AsPC1 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Antiproliferative activity against human AsPC1 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 24904966]
DU-145	IC₅₀	2.5 μM Compound: Stattic	Cytotoxicity against human DU145 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human DU145 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26396689]
HCT-116	IC₅₀	1.08 μM Compound: Stattic	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 27718470]
HCT-116	IC₅₀	2.16 μM Compound: Stattic	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
HEL	IC₅₀	1.59 μM Compound: Stattic	Antiproliferative activity against human HEL cells harboring JAK2 V617F mutant assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human HEL cells harboring JAK2 V617F mutant assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 33689932]
HepG2	IC₅₀	3.52 μM Compound: Stattic	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
L02	IC₅₀	5.2 μM Compound: Stattic	Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human LO2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
MCF-10A	IC₅₀	6.11 μM Compound: Stattic	Cytotoxicity against human MCF10A cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF10A cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
MCF7	IC₅₀	1.7 μM Compound: Stattic	Antiproliferative activity against human MCF7 cells expressing low levels of STAT3 assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells expressing low levels of STAT3 assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 33689932]
MCF7	IC₅₀	2.16 μM Compound: Stattic	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26396689]
MCF7	IC₅₀	2.36 μM Compound: Stattic	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 27718470]
MCF7	IC₅₀	3.6 μM Compound: Stattic	Antiproliferative activity against ER-positive human MCF7 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Antiproliferative activity against ER-positive human MCF7 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 24904966]
MCF7	IC₅₀	3.71 μM Compound: Stattic	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
MDA-MB-231	IC₅₀	1.56 μM Compound: Stattic	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26396689]
MDA-MB-231	IC₅₀	1.68 μM Compound: Stattic	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 27718470]
MDA-MB-231	IC₅₀	1.81 μM Compound: Stattic	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31048139]
MDA-MB-231	IC₅₀	2.64 μM Compound: Stattic	Antiproliferative activity against human MDA-MB-231 cells overexpressing STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MDA-MB-231 cells overexpressing STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 32603980]
MDA-MB-231	IC₅₀	2.89 μM Compound: Stattic	Antiproliferative activity against ER-negative and triple-negative human MDA-MB-231 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Antiproliferative activity against ER-negative and triple-negative human MDA-MB-231 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 24904966]
MDA-MB-435S	IC₅₀	1.87 μM Compound: Stattic	Cytotoxicity against human MDA-MB-435S cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-435S cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26396689]
MDA-MB-453	IC₅₀	4.4 μM Compound: Stattic	Antiproliferative activity against human MDA-MB-453 cells expressing low levels of STAT3 assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-453 cells expressing low levels of STAT3 assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 33689932]
MDA-MB-468	IC₅₀	1.92 μM Compound: Stattic	Antiproliferative activity against human MDA-MB-468 cells overexpressing STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MDA-MB-468 cells overexpressing STAT3 assessed as reduction in cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 32603980]
MDA-MB-468	IC₅₀	1.92 μM Compound: Stattic	Antiproliferative activity against human MDA-MB-468 cells overexpressing STAT3 assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells overexpressing STAT3 assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 33689932]
PANC-1	IC₅₀	2.9 μM Compound: Stattic	Cytotoxicity against human PANC1 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human PANC1 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26396689]
PANC-1	IC₅₀	3.77 μM Compound: Stattic	Antiproliferative activity against human PANC1 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Antiproliferative activity against human PANC1 cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 24904966]

体外研究
(In Vitro)

Stattic (20 μM；24 小时) 抑制 STAT3 磷酸化 (Y705) 并选择性抑制 P-STAT3，正如在 Panc-1 和 HPAC 胰腺癌细胞系的 ALDH⁺ 和 D44⁺/CD24⁺ 亚群中，缺乏对 P-ERK1/2 的抑制所证明的那样^[1]。
Stattic (2.5、5、10 μM；4 小时) 在 10 μM 时显著降低 PC3M-1E8 细胞中 pSTAT3 和存活蛋白的核水平。Stattic (2.5-10 μM；24 小时) 以剂量依赖性方式抑制 IL-6 诱导的 STAT3 激活^[2]。
Stattic (2.5，5，10 μM；48 小时) 在 10 μM 时抑制前列腺癌细胞 (PC3M-1E8 细胞) 的生长并诱导其凋亡。Stattic 在 2.5 μM、5 μM 时不会诱导显著的细胞凋亡^[2]。
Stattic (2.5，5，10 μM；48 小时) 显示出显著的 S 期积累^[2]。
Stattic 在 A2780 细胞和 HUVEC 细胞中不会导致显著的形态学改变或细胞凋亡，并且导致 STAT3 磷酸化水平很低^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Stattic (10 mg/kg；腹腔注射；每周 3 次，持续 10 周) 改善 Alport 综合征 (AS) 小鼠的肾功能障碍^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Age-matched wild-type (WT) C57BL/6 mice^[3]
Dosage:	10 mg/kg
Administration:	IP; three timesper week for 10 week
Result:	Increased levels of proteinuria, BUN and serum creatinine. Significantly suppressed the gene expression levels of renal injury markers (Lcn2, Kim-1), pro-inflammatory cytokines (Il-6, KC), pro-fibrotic genes (Tgf-β, Col1a1, α-Sma) and Mmp9.

分子量

211.19

Formula

C₈H₅NO₄S

CAS 号

19983-44-9

性状

固体

颜色

Off-white to yellow

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (236.75 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.7351 mL	23.6754 mL	47.3507 mL
5 mM	0.9470 mL	4.7351 mL	9.4701 mL
10 mM	0.4735 mL	2.3675 mL	4.7351 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.84 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.84 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： 50% PEG300 50% Saline
Solubility: 1.67 mg/mL (7.91 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.60%

选择批次：

Data Sheet (646 KB) SDS (393 KB)

COA (290 KB) HNMR (238 KB) RP-HPLC (245 KB) 元素分析报告 (214 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Lin L, et al. STAT3 as a potential therapeutic target in ALDH+ and CD44+/CD24+ stem cell-like pancreatic cancer cells. Int J Oncol. 2016 Oct 12. [Content Brief]

[2]. John S McMurray, et al. A new small-molecule Stat3 inhibitor. Chem Biol. 2006 Nov;13(11):1123-4. [Content Brief]

[3]. Tsubasa Yokota, et al. STAT3 inhibition attenuates the progressive phenotypes of Alport syndrome mouse model. Nephrol Dial Transplant. 2018 Feb 1;33(2):214-223. [Content Brief]

Stattic 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.7351 mL	23.6754 mL	47.3507 mL	118.3768 mL
	5 mM	0.9470 mL	4.7351 mL	9.4701 mL	23.6754 mL
	10 mM	0.4735 mL	2.3675 mL	4.7351 mL	11.8377 mL
	15 mM	0.3157 mL	1.5784 mL	3.1567 mL	7.8918 mL
	20 mM	0.2368 mL	1.1838 mL	2.3675 mL	5.9188 mL
	25 mM	0.1894 mL	0.9470 mL	1.8940 mL	4.7351 mL
	30 mM	0.1578 mL	0.7892 mL	1.5784 mL	3.9459 mL
	40 mM	0.1184 mL	0.5919 mL	1.1838 mL	2.9594 mL
	50 mM	0.0947 mL	0.4735 mL	0.9470 mL	2.3675 mL
	60 mM	0.0789 mL	0.3946 mL	0.7892 mL	1.9729 mL
	80 mM	0.0592 mL	0.2959 mL	0.5919 mL	1.4797 mL
	100 mM	0.0474 mL	0.2368 mL	0.4735 mL	1.1838 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Stattic19983-44-9STATApoptosisp-STAT3Y705S727SH2AlportsyndromeP-ERK1/2PC3M-1E8prostatecancerS phaseInhibitorinhibitorinhibit

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Stattic

Customer Review

MCE 顾客使用本产品发表的 175 篇科研文献

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参考文献

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完整储备液配制表

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Stattic

Customer Review

Stattic 相关抗体

MCE 顾客使用本产品发表的 175 篇科研文献

Stattic 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 STAT 亚型特异性产品:

生物活性

纯度 & 产品资料

参考文献

Stattic 相关抗体:

摩尔计算器

稀释计算器

Stattic 相关分类

完整储备液配制表

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您最近查看的产品:

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