2. Anti-infection
  3. Bacterial Antibiotic Parasite
  4. Aflatoxin B2

Aflatoxin B2  (Synonyms: 黄曲霉毒素 B2)

目录号: HY-N6696 纯度: 99.28%
COA 产品使用指南 技术支持

Aflatoxin B2 是一种口服有效的肝毒性霉菌毒素，是肝细胞的凋亡/自噬 (apoptosis/autophagy) 诱导剂。Aflatoxin B2 通过增加 ROS 生成、促进 Bax 和细胞色素 C 的转位、激活 caspase-3 和 PARP 切割，触发线粒体介导的细胞凋亡。Aflatoxin B2 同时通过抑制 PI3K/Akt/mTOR 信号通路，上调 Beclin-1 和 LC3-II/LC3-I 并下调 p62，诱导自噬。Aflatoxin B2 可用于构建肝损伤模型。

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Aflatoxin B2

Aflatoxin B2 Chemical Structure

CAS No. : 7220-81-7

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Customer Review

Other Forms of Aflatoxin B2:

Aflatoxin B2 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Aflatoxin B2 is an orally active hepatotoxic mycotoxin and an inducer of apoptosis/autophagy in hepatocytes. Aflatoxin B2 triggers mitochondria-mediated apoptosis by increasing ROS generation, promoting the translocation of Bax and cytochrome C, activating caspase-3 and PARP cleavage. Aflatoxin B2 also induces autophagy by inhibiting the PI3K/Akt/mTOR signaling pathway, upregulating Beclin-1 and LC3-II/LC3-I, and downregulating p62. Aflatoxin B2 can be used to construct a liver injury model[1][2][3].

体外研究
(In Vitro)

Aflatoxin B2 (10-100 μM；24-48 h) 在 J774A.1 小鼠巨噬细胞中表现出剂量依赖性的细胞毒性，仅 100 μM 时显著抑制细胞活力。Aflatoxin B2 通过线粒体介导的途径增加凋亡细胞比例并激活 caspase-3 和 PARP 切割[1]。
Aflatoxin B2 (0.1 ng/mL；72 h) 与 100 ng/mL LPS 协同处理细胞后，显著增加小鼠巨噬细胞 J774A.1 中促炎因子 IL-6 的分泌水平[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Aflatoxin B2 相关抗体:

Cell Viability Assay[1]

Cell Line: J774A.1 murine macrophages
Concentration: 10 μM, 50 μM, 100 μM
Incubation Time: 24 h and 48 h
Result: MTT assay showed no significant cytotoxicity at 10-50 μM, while 100 μM Aflatoxin B2 significantly reduced cell viability by 30% at 48 h, indicating dose-dependent cytotoxicity at high concentrations.
Flow cytometry with Annexin V/PI staining revealed no significant increase in apoptotic cells at 10-100 μM, suggesting cytotoxicity was not mediated by classical apoptotic pathways.
Western blot analysis showed no significant changes in caspase-3 or PARP cleavage at 10-50 μM, consistent with the lack of apoptotic induction.
体内研究
(In Vivo)

Aflatoxin B2 (0.2-0.8 mg/kg；饲料；每日一次；21 d) 在肉鸡肝细胞中通过抑制 PI3K/Akt/mTOR 信号通路，上调 Beclin-1 和 LC3-II/LC3-I 比值，下调 p62，诱导自噬，并通过增加 ROS 生成、促进 Bax 和细胞色素 c 转位，激活线粒体凋亡通路[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Cobb500 broilers (1-day-old, 40-50 g)[3]
Dosage: 0.2 mg/kg, 0.4 mg/kg, 0.8 mg/kg (add in feed)
Administration: Oral administration via feed, once daily, for 21 consecutive days
Result: Dose-dependent increased in apoptotic hepatocytes with nuclear fragmentation and chromatin condensation. Revealed crescent-shaped nuclei and mitochondrial swelling.
Activated cleaved caspase-3 and PARP, indicating activation of the mitochondrial apoptotic pathway. Intracellular reactive oxygen species (ROS) levels increased significantly, promoting Bax translocation to mitochondria and cytochrome c release into the cytosol, forming apoptosomes with Apaf-1 and caspase-9.
Enhanced MDC staining and LC3 immunofluorescence formation of autophagic vacuoles and LC3 puncta. Upregulated Beclin-1, increased LC3-II/LC3-I ratio, and downregulated p62, confirming autophagic flux.
Inhibited the PI3K/Akt/mTOR signaling pathway, as evidenced by reduced phosphorylation of PI3K, Akt, and mTOR (p-PI3K, p-Akt, p-mTOR S2448) and upregulated PTEN expression.
Upregulated Bax mRNA and downregulated Bcl-2 mRNA, while Western blot of mitochondrial and cytosolic fractions revealed increased Bax in mitochondria and cytochrome c in the cytosol, supporting mitochondrial pathway activation.
分子量

314.29

Formula

C17H14O6
CAS 号
性状

固体

颜色

White to off-white

中文名称

黄曲霉毒素 B2
结构分类
初始来源

Aspergillus flavus and Aspergillus parasiticus
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 2.94 mg/mL (9.35 mM; 超声助溶 (<80°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.1818 mL 15.9089 mL 31.8177 mL
5 mM 0.6364 mL 3.1818 mL 6.3635 mL
查看完整储备液配制表

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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体积
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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体积 (start)

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浓度 (final)

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动物溶解方案计算器
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工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.41%

COA (302 KB) LCMS (267 KB)

产品使用指南 (1538 KB)
参考文献

Aflatoxin B2 相关分类

完整储备液配制表

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1818 mL 15.9089 mL 31.8177 mL 79.5444 mL
5 mM 0.6364 mL 3.1818 mL 6.3635 mL 15.9089 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Aflatoxin B27220-81-7黄曲霉毒素 B2Aflatoxin B 2Aflatoxin B-2BacterialAntibioticParasiteInhibitorinhibitorinhibit

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