2. Immunology/Inflammation Apoptosis
  3. Toll-like Receptor (TLR) Apoptosis
  4. Polyinosinic-polycytidylic acid sodium

Polyinosinic-polycytidylic acid sodium  (Synonyms: Poly(I:C) sodium)

目录号: HY-135748 含量: 99.4%
COA 产品使用指南 技术支持

Polyinosinic-polycytidylic acid sodium (Poly(I:C)) sodium 是双链 RNA 的合成类似物，是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Polyinosinic-polycytidylic acid sodium 可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡。

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Polyinosinic-polycytidylic acid sodium Chemical Structure

Polyinosinic-polycytidylic acid sodium Chemical Structure

CAS No. : 42424-50-0

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规格 价格 是否有货 数量
1 mg ￥248
In-stock
5 mg ￥560
In-stock
10 mg ￥915
In-stock
25 mg ￥1730
In-stock
50 mg ￥2590
In-stock
100 mg ￥3900
In-stock
500 mg 现货 询价
1 g   询价  
5 g   询价  

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Customer Review

Other Forms of Polyinosinic-polycytidylic acid sodium:

Polyinosinic-polycytidylic acid sodium 相关抗体

Top Publications Citing Use of Products

    Polyinosinic-polycytidylic acid sodium purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    BECN1 knockout HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid sodium purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HeLa cells were transfected with pCAGGS, HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB, IFIT1 and CXCL10 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid sodium purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with HA-8A or HA-NSs at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. Co-IP with HA antibodies and evaluation of LC3, TBK1 and TRIM25 with western blot using IgG as control.

    Polyinosinic-polycytidylic acid sodium purchased from MCE. Usage Cited in: Autophagy. 2024 May 18.  [Abstract]

    HEK293T cells were transfected with pCAGGS, HA-NSs and HA-8A, HA-PxxP, HA-DWP, or HA-WxxL at a dose of 2 μg for 24 h, and then transfected with poly(I:C) for 24 h. IFNB and IFIT1 mRNA levels were analyzed with RT-qPCR.

    Polyinosinic-polycytidylic acid sodium purchased from MCE. Usage Cited in: Chem Eng J. 2021 Aug 15;418:129392.

    Immunofluorescence staining photographs of lung sections. Scale bars, 100 μm. (I: PBS, II: Poly(I:C), III: PL-Poly(I:C), IV: NL-Poly(I:C), V: PL-Poly(I:C)/RBD, VI: NL-Poly(I:C)/RBD).

    查看 Toll-like Receptor (TLR) 亚型特异性产品:

    查看所有亚型
    TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Polyinosinic-polycytidylic acid (Poly(I:C)) sodium is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid sodium can directly trigger cancer cells to undergo apoptosis[1][2].

    IC50 & Target

    TLR3

     

    体外研究
    (In Vitro)

    Polyinosinic-polycytidylic acid（20 ng/mL；24 小时；WM793、WM278、WM239A、WM9 和 1205Lu 细胞）处理细胞后，可在 24 小时内显着降低存活率，从对照的 100% 降至 20%-50%[1].
    Polyinosinic-polycytidylic acid（200 ng/mL；24 小时；1205Lu 细胞）处理诱导 1205Lu 细胞凋亡[1]。
    Polyinosinic-polycytidylic acid（3 ng/mL；24 小时；1205Lu 细胞）处理诱导黑色素瘤细胞中的 IFN-β 表达。 RIG-I 和 MDA-5 的沉默证实 Polyinosinic-polycytidylic acid 诱导 IFN-β 分别需要 RIG-I 和 MDA-5，并且需要 IPS-1[1]。< br/> Polyinosinic-polycytidylic acid（5 ng/mL；24 小时；1205Lu 细胞）处理揭示黑素瘤细胞中 caspase-9 和 caspase-8 的活性亚基[1]。
    Polyinosinic-polycytidylic acid 的钠盐，通过重悬于无菌盐水中制备用于注射，以 2 mg/mL 的浓度加热至 50 °C 以确保完全溶解，然后自然冷却至室温以确保双链 RNA 的适当退火。Polyinosinic-polycytidylic acid 需储存在 -20 °C 直至使用[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Polyinosinic-polycytidylic acid sodium 相关抗体:

    Cell Viability Assay[1]

    Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells
    Concentration: 20 ng/mL
    Incubation Time: 24 hours
    Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours.

    Apoptosis Analysis[1]

    Cell Line: 1205Lu cells
    Concentration: 200 ng/mL
    Incubation Time: 24 hours
    Result: Induced apoptosis in 1205Lu cells.

    RT-PCR[1]

    Cell Line: 1205Lu cells
    Concentration: 3 ng/mL
    Incubation Time: 24 hours
    Result: Induced IFN-β expression in melanoma cells.

    Western Blot Analysis[1]

    Cell Line: 1205Lu cells
    Concentration: 5 ng/mL
    Incubation Time: 24 hours
    Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.
    体内研究
    (In Vivo)

    Polyinosinic-polycytidylic acid 可抑制注射 1205Lu 细胞的 NOD/SCID 免疫缺陷小鼠的肿瘤生长。用 Polyinosinic-polycytidylic acid 处理的小鼠后，人类 DNA 的水平降低了 50%[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Formula

    (C10H13N4O8P)x.(C9H14N3O8P)x.xNa
    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 10 mg/mL (超声加热助溶)

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 6.67 mg/mL; 澄清溶液; 超声助溶 (<60°C)

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.4%

    COA (260 KB)

    产品使用指南 (1538 KB)
    参考文献

    Polyinosinic-polycytidylic acid sodium 相关分类

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Polyinosinic-polycytidylic acid42424-50-0Poly(I:C)Toll-like Receptor (TLR)ApoptosisTLR3RIG-IMDA5immunotherapytumormicroenvironment apoptosisdsRNAproperannealingdouble-strandedRNAInhibitorinhibitorinhibit

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