Scoulerine (Synonyms: (-)-Scoulerine; Discretamine)

目录号: HY-N1255 纯度: 99.27%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Scoulerine ((-)-Scoulerine) 是一种有效的抗有丝分裂 (antimitotic) 化合物。Scoulerine 也是 BACE1 (淀粉样前体蛋白裂解酶 1) 的抑制剂。Scoulerine 抑制细胞增殖，阻止细胞周期，并诱导癌细胞凋亡。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Scoulerine Chemical Structure

CAS No. : 6451-73-6

1. 客户无需承担相应的运输费用。

2. 同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

可免费申领三个不同产品的试用装。

3. 试用装只面向终端客户。

规格	价格	是否有货
1 mg	￥550	In-stock
5 mg	￥2000	In-stock
10 mg	￥3500	In-stock
50 mg		询价
100 mg		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

Scoulerine 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 Beta-secretase 亚型特异性产品:

查看所有亚型

BACE1 BACE2

生物活性
纯度 & 产品资料
参考文献

生物活性

Scoulerine ((-)-Scoulerine), an isoquinoline alkaloid, is a potent antimitotic compound. Scoulerine is also an inhibitor of BACE1 (?-site amyloid precursor protein cleaving enzyme 1). Scoulerine inhibits proliferation, arrests cell cycle, and induces apoptosis in cancer cells^[1].

IC₅₀ & Target

BACE1

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	26.32 μM Compound: (-)-scoulerine	Cytotoxicity against human A549 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
GES1	IC₅₀	95.3 μM Compound: 4	Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HeLa	IC₅₀	6.737 μM Compound: (-)-scoulerine	Cytotoxicity against human HeLa cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
HGC-27	IC₅₀	6.1 μM Compound: 4	Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HRPE	EC₅₀	3.545 μM Compound: (-)-scoulerine	Induction of mitotic arrest in HRPE Cells overexpressing MYC measured for 24 hrs by acridine orange staining based fluorescence microscopic analysis Induction of mitotic arrest in HRPE Cells overexpressing MYC measured for 24 hrs by acridine orange staining based fluorescence microscopic analysis	[PMID: 34406008]
HRPE	EC₅₀	3.595 μM Compound: (-)-scoulerine	Induction of mitotic arrest in HRPE Cells overexpressing MYC assessed as polyploidy measured for 48 hrs by acridine orange staining based fluorescence microscopic analysis Induction of mitotic arrest in HRPE Cells overexpressing MYC assessed as polyploidy measured for 48 hrs by acridine orange staining based fluorescence microscopic analysis	[PMID: 34406008]
HRPE	IC₅₀	4.065 μM Compound: (-)-scoulerine	Cytotoxicity against HRPE cells overexpressing MYC assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against HRPE cells overexpressing MYC assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
HRPE	IC₅₀	4.622 μM Compound: (-)-scoulerine	Cytotoxicity against HRPE cells overexpressing MYC/Bcl2 assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against HRPE cells overexpressing MYC/Bcl2 assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
MDA-MB-231	IC₅₀	7.324 μM Compound: (-)-scoulerine	Cytotoxicity against human MDA-MB-231 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
MGC-803	IC₅₀	12.6 μM Compound: 4	Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
NCI-H23	IC₅₀	2.541 μM Compound: (-)-scoulerine	Cytotoxicity against human NCI-H23 cells harboring MYC assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human NCI-H23 cells harboring MYC assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
SW480	IC₅₀	4.237 μM Compound: (-)-scoulerine	Cytotoxicity against human SW480 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human SW480 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]

体外研究
(In Vitro)

Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC₅₀s ranging from 2.7?μM to 6.5?μM^[1].
Scoulerine (2.5-20?μM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells^[1].
Scoulerine (2.5-20?μM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis^[1].
Scoulerine induces G2 or M cell cycle arrest^[1].
Scoulerine (2.5-5?μM; 24?hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells^[1].
Scoulerine (2.5-5?μM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner^[1].
Scoulerine (5-10 μM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells^[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Jurkat and MOLT-4 cells
Concentration:	2.5, 5, 10, 15 and 20 µM
Incubation Time:	24 hours
Result:	Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner.

Apoptosis Analysis^[1]

Cell Line:	MOLT-4 and Jurkat cells
Concentration:	2.5, 5, 10, 15 and 20 µM
Incubation Time:	24 hours
Result:	Induced MOLT-4 and Jurkat cells apoptosis.

Cell Cycle Analysis^[1]

Cell Line:	Jurkat and MOLT-4 leukemic cells
Concentration:	2.5-20 µM
Incubation Time:	16 hours
Result:	Induced cell cycle arrest at the G2/M transition.

Western Blot Analysis^[1]

Cell Line:	MOLT-4 cells
Concentration:	2.5, 5 µM
Incubation Time:	24 hours
Result:	Showed an upregulation of p53 protein in p53 wild-type MOLT-4 cells.

分子量

327.37

Formula

C₁₉H₂₁NO₄

CAS 号

6451-73-6

性状

固体

颜色

Off-white to pink

中文名称

金黄紫堇碱

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 100 mg/mL (305.46 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.0546 mL	15.2732 mL	30.5465 mL
5 mM	0.6109 mL	3.0546 mL	6.1093 mL
10 mM	0.3055 mL	1.5273 mL	3.0546 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.35 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.35 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.27%

选择批次：

Data Sheet (623 KB) SDS (393 KB)

COA (264 KB) HNMR (122 KB) RP-HPLC (132 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Habartova K, et al. Scoulerine affects microtubule structure, inhibits proliferation, arrests cell cycle and thus culminates in the apoptotic death of cancer cells. Sci Rep. 2018;8(1):4829. Published 2018 Mar 19. [Content Brief]

Scoulerine 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0546 mL	15.2732 mL	30.5465 mL	76.3662 mL
	5 mM	0.6109 mL	3.0546 mL	6.1093 mL	15.2732 mL
	10 mM	0.3055 mL	1.5273 mL	3.0546 mL	7.6366 mL
	15 mM	0.2036 mL	1.0182 mL	2.0364 mL	5.0911 mL
	20 mM	0.1527 mL	0.7637 mL	1.5273 mL	3.8183 mL
	25 mM	0.1222 mL	0.6109 mL	1.2219 mL	3.0546 mL
	30 mM	0.1018 mL	0.5091 mL	1.0182 mL	2.5455 mL
	40 mM	0.0764 mL	0.3818 mL	0.7637 mL	1.9092 mL
	50 mM	0.0611 mL	0.3055 mL	0.6109 mL	1.5273 mL
	60 mM	0.0509 mL	0.2546 mL	0.5091 mL	1.2728 mL
	80 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9546 mL
	100 mM	0.0305 mL	0.1527 mL	0.3055 mL	0.7637 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

产品名称：

* 需求量：

* 客户姓名：

* Email：

* 电话：

* 公司或机构名称：

留言给我们：

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。	站点地图隐私声明
沪ICP备15051369号-4 上海工商沪公网安备31011502019417 沪(浦)应急管危经许[2021]201709(QFYS) 营业执照（三证合一）
Copyright © 2013-2024 MedChemExpress. All Rights Reserved.

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Scoulerine (Synonyms: (-)-Scoulerine; Discretamine)

Customer Review