2. Immunology/Inflammation Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Neuronal Signaling Membrane Transporter/Ion Channel
  3. Glucocorticoid Receptor Endogenous Metabolite iGluR
  4. Corticosterone

Corticosterone  (Synonyms: 皮质酮; 17-Deoxycortisol; 11β,21-Dihydroxyprogesterone; Kendall's compound B)

目录号: HY-B1618 纯度: 99.76%
COA 产品使用指南

摩尔计算器

Corticosterone (17-Deoxycortisol) 是一种具有口服活性的，由肾上腺皮质产生的糖皮质激素，在调节边缘系统 (包括海马体、前额叶皮层和杏仁核) 的神经元功能方面起着重要作用。皮质酮可通过 SGK 诱导的 GDI 磷酸化增加 Rab 介导的 AMPAR 膜运输。Corticosterone 还能干扰树突状细胞的成熟，具有较好的免疫抑制活性。

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Corticosterone Chemical Structure

Corticosterone Chemical Structure

CAS No. : 50-22-6

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Other Forms of Corticosterone:

Corticosterone 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 33 篇科研文献

WB

    Corticosterone purchased from MCE. Usage Cited in: Sci Adv. 2022 Nov 11;8(45):eadd7063.  [Abstract]

    Corticosterone (CORT) (10 µM, 24 h) is used to produce a cell stress model on the primary culture hippocampal neurons from rats. The decreased NHE1 expression levels in primary cell culture induced by CORT treatment are not affected by chloroquine (Fig. D) but significantly reversed with MG132 pretreatment (Fig. E).

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    AMPA Receptor NMDA Receptor Kainate Receptor

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Corticosterone (17-Deoxycortisol) is an orally active and adrenal cortex-produced glucocorticoid, which plays an important role in regulating neuronal functions of the limbic system (including hippocampus, prefrontal cortex, and amygdala). Corticosterone increases the Rab-mediated AMPAR membrane traffic via SGK-induced phosphorylation of GDI. Corticosterone also interferes with the maturation of dendritic cells and shows a good immunosuppressive effect[1][2][3][4].

    IC50 & Target

    Human Endogenous Metabolite

     

    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    HEK293 IC50
    1.6 μM
    Compound: corticosterone
    Inhibition of the Organic Cation Transporter 3 (OCT3, SLC22A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by MPP+ uptake in HEK-293 JumpIN-SLC22A3 cells
    Inhibition of the Organic Cation Transporter 3 (OCT3, SLC22A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by MPP+ uptake in HEK-293 JumpIN-SLC22A3 cells
    		[PMID: 35163125]
    体外研究
    (In Vitro)

    Corticosterone (100 nM; 30 min) via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane[1].
    Corticosterone (CORT) (1 µM; 48 h) shows good immunosuppressive properties (functionally compromises maturation of BMDC), which impairs LPS-induced up-regulation of maturation-associated markers (MHC class II, B7.2, B7.1 and CD40)[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Corticosterone 相关抗体:

    Cell Viability Assay[1]

    Cell Line: HEK293 cells
    Concentration: 100 nM
    Incubation Time: 30 min
    Result: Caused a significant enhancement of mEPSC amplitude (mEPSC represents the postsynaptic response to release of individual vesicles of glutamate).
    Increased the transmission of glutamatergic, and increased synaptic AMPAR currents via a Rab4-dependent mechanism.
    Profoundly increased surface GluR1 cluster density, cluster size and cluster fluorescence intensity.
    Significantly increased the amount of Rab4 that binded to WT-GDI, S45A-GDI, or S121A-GDI but not S213A-GDI.
    Induced the phosphorylation of GST-WTGDI, GST-S45AGDI, and GST-S121AGDI, but not GST-S213AGDI, and this effect was blocked in cells transfected with SGK1 small interfering RNA.
    Increased AMPAR surface expression via a mechanism dependent on GDI phosphorylation.

    Cell Viability Assay[2]

    Cell Line: BMDC cells
    Concentration: 1 µM
    Incubation Time: 48 h
    Result: Completely blocked the expression of MHC class II and B7.2 that induced by LPS, and maximally impaired BMDC cells maturation at 12 h.
    Reduced B7.1 by 50%, and slightly down-regulated CD40.
    体内研究
    (In Vivo)

    Corticosterone results in a marked reduction in the ability of BMDC cells to prime naive CD8+ T cells in vivo[2].
    Corticosterone (0.03 or 1 mg/kg; s.c.; single) downregulates expression of BDNF mRNA in dentate gyrus and CA1 of rats[3].
    Corticosterone (2.6 mg/kg; in animal feedings; 8 days) restores ethanol intake and preference to approximately normal preoperative levels in adrenalectomy (ADX) rats[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Wistar rats (150-170 g; adrenalectomized)[3].
    Dosage: 0.03 or 1 mg/kg
    Administration: Subcutaneous injection; single.
    Result: Decreased expression of BDNF mRNA in dentate gyrus, with 25% and 50% lower for dosages of 0.03 and 1 mg/kg, respectively (3 h after administration).
    Reduced approximately 40% BDNF mRNA level as compared to the t=0 h control group (3 h after administration), but the level increased by 100% when 12 h after administration (compared to t=3 h and t=6 h group).
    Animal Model: Male Wistar rats (3-week-old; adrenalectomized)[4].
    Dosage: 2.6 mg/kg
    Administration: In animal feedings; 8 days.
    Result: Restored ethanol intake and preference of adrenalectomy (ADX) rats to approximately normal preoperative levels and to the levels observed in the sham-operated group (SH) rats.
    Clinical Trial
    分子量

    346.46

    Formula

    C21H30O4
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    皮质酮；皮质甾酮；皮质脂酮
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 100 mg/mL (288.63 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    Ethanol 中的溶解度 : 14.29 mg/mL (41.25 mM; 超声助溶)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.8863 mL 14.4317 mL 28.8634 mL
    5 mM 0.5773 mL 2.8863 mL 5.7727 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.22 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.22 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： 0.5% CMC-Na/saline water

      Solubility: 2 mg/mL (5.77 mM); 悬浊液; 超声助溶 (<60°C)

    • 方案 二

      请依序添加每种溶剂： 20% HP-β-CD in Saline

      Solubility: 4 mg/mL (11.55 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.76%

    COA (290 KB) HNMR (259 KB) RP-HPLC (246 KB) LCMS (93 KB)

    产品使用指南 (1538 KB)
    参考文献

    Corticosterone 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.8863 mL 14.4317 mL 28.8634 mL 72.1584 mL
    5 mM 0.5773 mL 2.8863 mL 5.7727 mL 14.4317 mL
    10 mM 0.2886 mL 1.4432 mL 2.8863 mL 7.2158 mL
    15 mM 0.1924 mL 0.9621 mL 1.9242 mL 4.8106 mL
    20 mM 0.1443 mL 0.7216 mL 1.4432 mL 3.6079 mL
    25 mM 0.1155 mL 0.5773 mL 1.1545 mL 2.8863 mL
    30 mM 0.0962 mL 0.4811 mL 0.9621 mL 2.4053 mL
    40 mM 0.0722 mL 0.3608 mL 0.7216 mL 1.8040 mL
    DMSO 50 mM 0.0577 mL 0.2886 mL 0.5773 mL 1.4432 mL
    60 mM 0.0481 mL 0.2405 mL 0.4811 mL 1.2026 mL
    80 mM 0.0361 mL 0.1804 mL 0.3608 mL 0.9020 mL
    100 mM 0.0289 mL 0.1443 mL 0.2886 mL 0.7216 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Corticosterone50-22-617-Deoxycortisol 11β,21-Dihydroxyprogesterone Kendall's compound B皮质酮皮质甾酮皮质脂酮Glucocorticoid ReceptorEndogenous MetaboliteiGluRIonotropic glutamate receptorsimmunosuppressionBMDCBDNFethanol intakeB7.2B7.1MHC class IICD40Inhibitorinhibitorinhibit

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