2. Neuronal Signaling Metabolic Enzyme/Protease Apoptosis
  3. Monoamine Oxidase COMT Apoptosis Endogenous Metabolite
  4. Rosmarinic acid

Rosmarinic acid  (Synonyms: 迷迭香酸; Labiatenic acid)

目录号: HY-N0529 纯度: 99.73%
COA 产品使用指南

摩尔计算器

Rosmarinic acid 是植物中广泛存在的一种酚酯化合物。Rosmarinic acid 抑制 MAO-A，MAO-B 和 COMT，IC50 分别为 50.1，184.6 和 26.7 μM。

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Rosmarinic acid Chemical Structure

Rosmarinic acid Chemical Structure

CAS No. : 20283-92-5

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥730
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50 mg ￥550
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100 mg ￥900
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500 mg   询价  

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Other Forms of Rosmarinic acid:

Rosmarinic acid 相关抗体

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MAO-A MAO-B

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Rosmarinic acid is a widespread phenolic ester compound in the plants. Rosmarinic acid inhibits MAO-A, MAO-B and COMT enzymes with IC50s of 50.1, 184.6 and 26.7 μM, respectively.

IC50 & Target

IC50: 50.1 μM (MAO-A), 184.6 μM (MAO-B), 26.7 μM (COMT)[1]
细胞效力
(Cellular Effect)
Cell Line Type Value Description References
HeLa IC50
0.96 μM
Compound: 5
Cytotoxicity against human HeLa cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
Cytotoxicity against human HeLa cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
[PMID: 36126331]
HeLa IC50
16.2 μM
Compound: 4
Inhibition of transcription factor AP-1 binding to oligonucleotide containing TPA-responsive element in TPA-activated human HeLa cells after 1 hr by ELISA
Inhibition of transcription factor AP-1 binding to oligonucleotide containing TPA-responsive element in TPA-activated human HeLa cells after 1 hr by ELISA
[PMID: 24491635]
HT-22 EC50
86 μM
Compound: 23
Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
[PMID: 32991171]
MOLM-13 IC50
2.9 μM
Compound: 19
Antiproliferative activity against human MOLM13 cells by Cell-Titer Glo assay
Antiproliferative activity against human MOLM13 cells by Cell-Titer Glo assay
[PMID: 30370766]
MOLM-14 IC50
7.1 μM
Compound: 19
Antiproliferative activity against human MOLM14 cells by Cell-Titer Glo assay
Antiproliferative activity against human MOLM14 cells by Cell-Titer Glo assay
[PMID: 30370766]
MT4 EC50
> 55 μM
Compound: rosmarinic acid, 1
Antiviral activity against HIV1 3B infected in human MT4 cells assessed as reduction of virus-induced cytopathogenicity by MTT assay
Antiviral activity against HIV1 3B infected in human MT4 cells assessed as reduction of virus-induced cytopathogenicity by MTT assay
[PMID: 18351727]
MT4 CC50
55 μM
Compound: rosmarinic acid, 1
Cytotoxicity against human MT4 cells by MTT method
Cytotoxicity against human MT4 cells by MTT method
[PMID: 18351727]
MV4-11 IC50
> 10 μM
Compound: 19
Antiproliferative activity against human MV4-11 cells by Cell-Titer Glo assay
Antiproliferative activity against human MV4-11 cells by Cell-Titer Glo assay
[PMID: 30370766]
PC-12 EC50
57.2 μM
Compound: rosmarinic acid
Inhibition of amyloid beta (1 to 42 residues) (unknown origin) aggregate-induced toxicity in rat PC12 cells assessed as cell viability at 4 to 100 ug/ml pre-incubated for 24 hrs with Abeta ( 1 to 42) followed by compound-Abeta (1 to 42) mixture addition t
Inhibition of amyloid beta (1 to 42 residues) (unknown origin) aggregate-induced toxicity in rat PC12 cells assessed as cell viability at 4 to 100 ug/ml pre-incubated for 24 hrs with Abeta ( 1 to 42) followed by compound-Abeta (1 to 42) mixture addition t
[PMID: 26517152]
PC-12 EC50
68.1 μM
Compound: rosmarinic acid
Inhibition of amyloid beta (25 to 35 residues) (unknown origin) aggregate-induced toxicity in rat PC12 cells preincubated for 1 hr followed by amyloid beta challenge measured after 24 hrs by MTT assay
Inhibition of amyloid beta (25 to 35 residues) (unknown origin) aggregate-induced toxicity in rat PC12 cells preincubated for 1 hr followed by amyloid beta challenge measured after 24 hrs by MTT assay
[PMID: 26517152]
PC-3 IC50
> 10 μM
Compound: 5
Cytotoxicity against human PC-3 cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
Cytotoxicity against human PC-3 cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
[PMID: 36126331]
RAW264.7 IC50
34.2 μM
Compound: 25
Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA
Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA
[PMID: 31747281]
Sf9 IC50
1.3 μM
Compound: Rosmarinic acid
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 1 umol/L ATP
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 1 umol/L ATP
[PMID: 17315853]
Sf9 IC50
17 μM
Compound: Rosmarinic acid
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 100 umol/L ATP
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 100 umol/L ATP
[PMID: 17315853]
Sf9 IC50
3.6 μM
Compound: Rosmarinic acid
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 10 umol/L ATP
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 10 umol/L ATP
[PMID: 17315853]
Sf9 IC50
36 μM
Compound: Rosmarinic acid
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 1 umol/L ATP
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 1 umol/L ATP
[PMID: 17315853]
Sf9 IC50
63 μM
Compound: Rosmarinic acid
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 100 umol/L ATP
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 100 umol/L ATP
[PMID: 17315853]
Sf9 IC50
74 μM
Compound: Rosmarinic acid
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 10 umol/L ATP
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 10 umol/L ATP
[PMID: 17315853]
SH-SY5Y IC50
> 10 μM
Compound: 5
Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
[PMID: 36126331]
体外研究
(In Vitro)

Rosmarinic acid (RA) 展现出以抗氧化作用为特点的多功能体外作用，并抑制单胺氧化酶 (MAO-A 和 MAO-B) 和儿茶酚-O-甲基转移酶 (COMT) 。Rosmarinic acid 显示对羟基自由基 (HO (?) ) 和一氧化氮自由基 (NO) 的抗氧化作用 (IC50 分别为 29.4 和 140 μM) ，以及抑制脂质过氧化 (IC50 为 19.6 μM) [1]。Rosmarinic acid (RA) 通过清除 UVB 诱导的细胞内活性氧 (ROS) 显示显著的细胞保护作用。在 H2O2 处理的细胞中，2.5 μM Rosmarinic acid 清除 60% 的细胞内 ROS，而 N-乙酰基-L-半胱氨酸 (NAC) 清除效果为 77%[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Rosmarinic acid 相关抗体:
体内研究
(In Vivo)

Rosmarinic acid (RA) 是一种广泛存在于植物中的酚酸酯化合物，尤其是 Labiatae 科的草本植物，如 Rosmarinus officinali、Salvia miltiorrhiza 和 Prunella vulgaris。Rosmarinic acid 通过双重抑制 NF-κB 和 STAT3 激活，抑制了葡聚糖硫酸钠 (DSS) 诱导的小鼠结肠炎。在 DSS 诱导的结肠炎模型中，Rosmarinic acid (30、60 mg/kg, p.o.) 显著减轻了细胞因子的产生 [3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

360.31

Formula

C18H16O8
CAS 号
性状

固体

颜色

Light yellow to light brown

中文名称

迷迭香酸；酪萨维；罗丹酚酸；肉桂醇甙
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 62.5 mg/mL (173.46 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7754 mL 13.8769 mL 27.7539 mL
5 mM 0.5551 mL 2.7754 mL 5.5508 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.77 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.77 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.73%

COA (285 KB) HNMR (285 KB) LCMS (93 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[2]

Human keratinocytes (HaCaT cells) are treated with Rosmarinic acid (0.625, 1.25, 2.5, or 5 μM) and exposed to UVB radiation 1 h later. They are then incubated at 37°C for 48 h. At this time, MTT is added to each well to obtain a total reaction volume of 200 μL. After 4 h incubation, the supernatant is removed by aspiration. The formazan crystals in each well are dissolved in dimethyl sulfoxide (DMSO; 150 μL), and the absorbance at 540 nm is measured on a scanning multi-well spectrophotometer[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[3]

Mice[3]
Experimental colitis is induced by giving mice drinking water ad libitum containing 5% (w/v) DSS for 7 days. Mice of each of the groups are monitored carefully every day to confirm that they have consumed an approximately equal volume of water containing DSS. For each experiment, the mice are divided into five experimental groups (n = 10/group). The first group is kept as the vehicle-treated control, and the second group is given drinking water with DSS only during the experimental period. The other three groups consist of mice receiving 5% DSS who are administrated 5-ASA (75 mg/kg/day p.o.) or Rosmarinic acid (30 or 60 mg/kg/day p.o.) daily for 7 days. Control groups are given the vehicle daily for 7 days as appropriate. Administration of each drug is initiated simultaneously with the DSS treatment.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Rosmarinic acid 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7754 mL 13.8769 mL 27.7539 mL 69.3847 mL
5 mM 0.5551 mL 2.7754 mL 5.5508 mL 13.8769 mL
10 mM 0.2775 mL 1.3877 mL 2.7754 mL 6.9385 mL
15 mM 0.1850 mL 0.9251 mL 1.8503 mL 4.6256 mL
20 mM 0.1388 mL 0.6938 mL 1.3877 mL 3.4692 mL
25 mM 0.1110 mL 0.5551 mL 1.1102 mL 2.7754 mL
30 mM 0.0925 mL 0.4626 mL 0.9251 mL 2.3128 mL
40 mM 0.0694 mL 0.3469 mL 0.6938 mL 1.7346 mL
50 mM 0.0555 mL 0.2775 mL 0.5551 mL 1.3877 mL
60 mM 0.0463 mL 0.2313 mL 0.4626 mL 1.1564 mL
80 mM 0.0347 mL 0.1735 mL 0.3469 mL 0.8673 mL
100 mM 0.0278 mL 0.1388 mL 0.2775 mL 0.6938 mL
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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rosmarinic acid20283-92-5Labiatenic acid迷迭香酸酪萨维罗丹酚酸肉桂醇甙Monoamine OxidaseCOMTApoptosisEndogenous MetaboliteMAOCatechol-O-methyltransferaseInhibitorinhibitorinhibit

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