2. Immunology/Inflammation
  3. COX
  4. Rutaecarpine

Rutaecarpine  (Synonyms: 吴茱萸次碱; Rutecarpine)

目录号: HY-N0147 纯度: 99.00%
COA 产品使用指南

摩尔计算器

Rutaecarpine, 是吴茱萸中的生物碱，是COX-2的抑制剂，其IC50值为0.28 μM。

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Rutaecarpine Chemical Structure

Rutaecarpine Chemical Structure

CAS No. : 84-26-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥550
In-stock
5 mg ￥323
In-stock
10 mg ￥485
In-stock
50 mg ￥1200
In-stock
100 mg ￥1800
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Rutaecarpine:

Rutaecarpine 相关抗体

Top Publications Citing Use of Products

查看 COX 亚型特异性产品:

查看所有亚型
COX COX-1 COX-2 COX-3

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Rutaecarpine, an alkaloid of Evodia rutaecarpa, is an inhibitor of COX-2 with an IC50 value of 0.28 μM.

IC50 & Target[1]

COX-2

0.28 μM (IC50, in BMMC)

COX-1

8.7 μM (IC50, in BMMC)

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
CCD-18Co IC50
> 50 μM
Compound: 1
Antiproliferative activity against human CCD-18Co cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human CCD-18Co cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
CCD-841CoN IC50
> 50 μM
Compound: 1
Antiproliferative activity against human CCD-841CoN cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human CCD-841CoN cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
HCT-116 IC50
31.1 μM
Compound: 1
Antiproliferative activity against human HCT-116 cells harboring beta-catenin mutant assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human HCT-116 cells harboring beta-catenin mutant assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
HCT-15 IC50
> 50 μM
Compound: 1
Antiproliferative activity against human HCT-15 cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human HCT-15 cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
HEK293 EC50
2.06 μM
Compound: 2
Agonist activity at rat TRPV1 channel expressed in HEK293 cells assessed as induction of channel current at -60 mV holding potential by whole-cell patch-clamp method
Agonist activity at rat TRPV1 channel expressed in HEK293 cells assessed as induction of channel current at -60 mV holding potential by whole-cell patch-clamp method
[PMID: 27159637]
HeLa EC50
26.1 μM
Compound: 1a
Cytotoxicity against human HeLa cells by MTT assay
Cytotoxicity against human HeLa cells by MTT assay
[PMID: 28958621]
LS174T IC50
6.1 μM
Compound: 1
Antiproliferative activity against human LS174T cells harboring beta-catenin mutant assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human LS174T cells harboring beta-catenin mutant assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
RKO IC50
> 50 μM
Compound: 1
Antiproliferative activity against human RKO cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human RKO cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
SW480 IC50
> 50 μM
Compound: 1
Antiproliferative activity against human SW480 cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human SW480 cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
体外研究
(In Vitro)

Rutaecarpine 具有多种有趣的生物学特性，如抗血栓形成、抗癌、抗炎和镇痛、抗肥胖和体温调节、血管舒张活性，以及对心血管和内分泌系统的影响[2]。
Rutaecarpine 以浓度依赖性方式抑制 BMMC 中 PGD2 生成的 COX-2 和 COX-1 依赖阶段，IC50 分别为 0.28 μM 和 8.7 μM。它以剂量依赖的方式抑制 COX-2 转染的 HEK293 细胞依赖于 COX-2 的外源性花生四烯酸向 PGE2 的转化[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Rutaecarpine 相关抗体:
体内研究
(In Vivo)

Rutaecarpine 在大鼠由λ-卡拉胶诱导的足跖水肿模型中通过腹腔注射显示出体内抗炎活性[1]。
Rutaecarpine以剂量依赖方式显著减少了抗体形成细胞的数量并导致脾脏重量下降。此外，给予Rutaecarpine的小鼠表现出脾脏细胞数量减少，脾脏中总T细胞、CD4+细胞、CD8+细胞和B细胞数量均减少。IL-2、干扰素和IL-10 mRNA的表达在rutaecarpine处理后被显著抑制。给予小鼠rutaecarpine后，CD4+IL-2+细胞的数量显著减少[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

287.32

Formula

C18H13N3O
CAS 号
性状

固体

颜色

Light yellow to yellow

中文名称

吴茱萸次碱
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 50 mg/mL (174.02 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.4804 mL 17.4022 mL 34.8044 mL
5 mM 0.6961 mL 3.4804 mL 6.9609 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献
Cell Assay
[1]

Rutaecarpine is dissolved in DMSO and diluted with appropriate medium before use. COX-1 and COX-2 cDNA-transfected HEK293 cells are prepared. For measuring inhibitory activity on COX-1 and COX-2 by rutaecarpine, cells in 1 mL of culture medium are seeded into each well of 24-well. After culture for 4 days, the supernatants are removed and 250 mL of fresh medium is added to the cells with or without rutaecarpine. After preincubation for 5 h at 37°C, the cells are further incubated at 37°C for 30 min with 50 mM arachidonic acid. All reactions are stopped by centrifugation at 120 g at 4°C for 5 min. Concentrations of PGE2 in the supernatant are measured[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1][3]

Rats: Rutaecarpine is dissolved in 0.1% carboxymethyl cellulose and diluted with appropriate medium before use. Male Splague-Dawley (SD) rats (180-220 g) are used in the study. Rutaecarpine administered intraperitoneally and, 1 h later, l-carrageenan solution is injected to right hind paw of rats. Paw volumes are measured using plethysmometer 5 h after l-carrageenan injection[1].

Mice: For the antibody response to SRBCs, rutaecarpine is administered at a single dose of 10 mg/kg, 20 mg/kg, 40 mg/kg or 80 mg/kg in 10 mL of 1% povidone solution intravenously. Control animals are given 1% povidone solution at 10 mL/kg. Specific pathogen-free female BALB/c mice are used in the study[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Rutaecarpine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4804 mL 17.4022 mL 34.8044 mL 87.0110 mL
5 mM 0.6961 mL 3.4804 mL 6.9609 mL 17.4022 mL
10 mM 0.3480 mL 1.7402 mL 3.4804 mL 8.7011 mL
15 mM 0.2320 mL 1.1601 mL 2.3203 mL 5.8007 mL
20 mM 0.1740 mL 0.8701 mL 1.7402 mL 4.3505 mL
25 mM 0.1392 mL 0.6961 mL 1.3922 mL 3.4804 mL
30 mM 0.1160 mL 0.5801 mL 1.1601 mL 2.9004 mL
40 mM 0.0870 mL 0.4351 mL 0.8701 mL 2.1753 mL
50 mM 0.0696 mL 0.3480 mL 0.6961 mL 1.7402 mL
60 mM 0.0580 mL 0.2900 mL 0.5801 mL 1.4502 mL
80 mM 0.0435 mL 0.2175 mL 0.4351 mL 1.0876 mL
100 mM 0.0348 mL 0.1740 mL 0.3480 mL 0.8701 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rutaecarpine84-26-4Rutecarpine吴茱萸次碱COXCyclooxygenaseInhibitorinhibitorinhibit

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