Tubercidin (Synonyms: 结核菌素; 7-Deazaadenosine)

目录号: HY-100126 纯度: ≥98.0%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Tubercidin (7-Deazaadenosine) 是一种从 Streptomyces tubercidicus 获得的抗生素，抑制粪链球菌 (8043) 生长的 IC₅₀ 值为 0.02 μM。Tubercidin 通过结合 DNA 或 RNA 抑制聚合酶，从而抑制 DNA 复制，RNA 和蛋白质合成。Tubercidin 是腺苷磷酸化酶的弱抑制剂，并干扰腺苷和 AMP 的磷酸化。Tubercidin 具有抗病毒活性。

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Tubercidin Chemical Structure

CAS No. : 69-33-0

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥797	In-stock
1 mg	￥272	In-stock
5 mg	￥600	In-stock
10 mg	￥900	In-stock
25 mg	￥1690	In-stock
50 mg	￥2500	In-stock
100 mg	￥3700	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

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生物活性
纯度 & 产品资料
参考文献

生物活性

Tubercidin (7-Deazaadenosine) is an antibiotic obtained from Streptomyces tubercidicus. Tubercidin inhibits the growth of Streptococcus faecalis (8043) with an IC₅₀ of 0.02 μM^[1]. Tubercidin inhibits polymerases by incorporating DNA or RNA, thereby inhibiting DNA replication, RNA and protein synthesis^[2]. Tubercidin is a weak inhibitor of adenosine phosphorylase, and interferes with the phosphorylation of adenosine and AMP^[1]. Tubercidin has antiviral activity^[2].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	GI₅₀	0.001 μM Compound: Tubercidin	Cytostatic activity against human A549 cells after 5 days by SRB assay Cytostatic activity against human A549 cells after 5 days by SRB assay	[PMID: 21711054]
DU-145	GI₅₀	0.018 μM Compound: Tubercidin	Cytostatic activity against human DU145 cells after 5 days by SRB assay Cytostatic activity against human DU145 cells after 5 days by SRB assay	[PMID: 21711054]
HCT-116	GI₅₀	0.001 μM Compound: Tubercidin	Cytostatic activity against human HCT116 cells after 5 days by SRB assay Cytostatic activity against human HCT116 cells after 5 days by SRB assay	[PMID: 21711054]
HCT-15	GI₅₀	0.011 μM Compound: Tubercidin	Cytostatic activity against human HCT15 cells after 5 days by SRB assay Cytostatic activity against human HCT15 cells after 5 days by SRB assay	[PMID: 21711054]
HEK293	IC₅₀	0.88 μM Compound: 4	Stabilization of Pdcd4 expressed in human HEK293 cells assessed as inhibition of TPA-induced degradation by luciferase reporter assay Stabilization of Pdcd4 expressed in human HEK293 cells assessed as inhibition of TPA-induced degradation by luciferase reporter assay	[PMID: 21870828]
HeLa S3	IC₅₀	0.03 μM Compound: 3	Antiviral activity against Poliovirus infected in human HeLaS3 cells assessed as inhibition of viral replication treated 1 hr before infection measured after 2 days Antiviral activity against Poliovirus infected in human HeLaS3 cells assessed as inhibition of viral replication treated 1 hr before infection measured after 2 days	[PMID: 20964406]
HeLa S3	IC₅₀	0.4 μM Compound: 3	Cytotoxicity against human HeLaS3 cells after 48 hrs by celltiter-glo assay Cytotoxicity against human HeLaS3 cells after 48 hrs by celltiter-glo assay	[PMID: 20964406]
HeLa S3	IC₅₀	0.819 μM Compound: 3	Cytotoxicity against human HeLaS3 cells after 24 hrs by celltiter-glo assay Cytotoxicity against human HeLaS3 cells after 24 hrs by celltiter-glo assay	[PMID: 20964406]
HeLa S3	IC₅₀	1.44 μM Compound: 3	Cytotoxicity against human HeLaS3 cells after 7 hrs by celltiter-glo assay Cytotoxicity against human HeLaS3 cells after 7 hrs by celltiter-glo assay	[PMID: 20964406]
KB	IC₅₀	1 μM Compound: 1	Cytotoxicity of compound was determined by assaying cell growth in human neoplastic cell line(KB). Cytotoxicity of compound was determined by assaying cell growth in human neoplastic cell line(KB).	[PMID: 1310744]
L1210	IC₅₀	0.04 μM Compound: 1	Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro. Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro.	[PMID: 1310744]
Macrophage	CC₅₀	0.13 μM Compound: Tubercidin	Cytotoxicity against Swiss mouse peritoneal macrophages assessed as reduction in cell viability measured after 5 days by microscopic analysis Cytotoxicity against Swiss mouse peritoneal macrophages assessed as reduction in cell viability measured after 5 days by microscopic analysis	[PMID: 35144125]
MRC5	EC₅₀	2.23 μM Compound: 1	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay	[PMID: 30677668]
MRC5	CC₅₀	2.23 μM Compound: Tubercidin	Cytotoxicity against human MRC5 fibroblast cells assessed as reduction in cell viability measured after 3 days by fluorometry Cytotoxicity against human MRC5 fibroblast cells assessed as reduction in cell viability measured after 3 days by fluorometry	[PMID: 35144125]
MT4	GI₅₀	0.021 μM Compound: Tubercidin	Cytostatic activity against human MT4 cells after 5 days by SRB assay Cytostatic activity against human MT4 cells after 5 days by SRB assay	[PMID: 21711054]
NCI-H23	GI₅₀	0.011 μM Compound: Tubercidin	Cytostatic activity against human NCI-H23 cells after 5 days by SRB assay Cytostatic activity against human NCI-H23 cells after 5 days by SRB assay	[PMID: 21711054]
PC-3	GI₅₀	0.048 μM Compound: Tubercidin	Cytostatic activity against human PC3 cells after 5 days by SRB assay Cytostatic activity against human PC3 cells after 5 days by SRB assay	[PMID: 21711054]
Vero	CC₅₀	> 357 μM Compound: 4; 7-DMA	Cytotoxicity against African green monkey Vero cells by visual method Cytotoxicity against African green monkey Vero cells by visual method	[PMID: 31549836]
Vero	EC₅₀	1.3 μM Compound: 4; 7-DMA	Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-induced cytopathic effect after 5 days by MTS assay Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-induced cytopathic effect after 5 days by MTS assay	[PMID: 31549836]
Vero	EC₅₀	1.3 μM Compound: 4; 7-DMA	Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by methylene blue staining based by Plaque reduction assay Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by methylene blue staining based by Plaque reduction assay	[PMID: 31549836]
Vero	EC₅₀	1.3 μM Compound: 4; 7-DMA	Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by Alexa Fluor 488/DAPI staining based assay Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by Alexa Fluor 488/DAPI staining based assay	[PMID: 31549836]
Vero	EC₅₀	1.3 μM Compound: 4; 7-DMA	Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-yield Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-yield	[PMID: 31549836]

体外研究
(In Vitro)

Tubercidin (7-Deazaadenosine) (0-10 nM；14 d) 对骨髓和红系人骨髓祖细胞具有剂量依赖性抑制作用，Tubercidin 对 CFU-GM 和 BFU-E 细胞的 IC₅₀ 分别为 3.4 nM 和 3.7 nM^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:	Human bone marrow progenitor cells
Concentration:	0-10 nM
Incubation Time:	14 days
Result:	Had a dose-dependent inhibitory effect for CFU-GM and BFU-E.

体内研究
(In Vivo)

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female CD1 mice^[2]
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; 5 mg/kg; 10 days
Result:	Protected the mice from the lethality of tubercidin.

分子量

266.25

Formula

C₁₁H₁₄N₄O₄

CAS 号

69-33-0

性状

固体

颜色

White to off-white

中文名称

杀结核菌素；结核菌素

结构分类

初始来源

微生物

Streptomyces tubercidicus

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 30 mg/mL (112.68 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.7559 mL	18.7793 mL	37.5587 mL
5 mM	0.7512 mL	3.7559 mL	7.5117 mL
10 mM	0.3756 mL	1.8779 mL	3.7559 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.39 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.39 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.39 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 98.68%

选择批次：

Data Sheet (639 KB) SDS (420 KB)

COA (200 KB) HNMR (181 KB) LCMS (399 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Bloch A, et al. On the mode of action of 7-deaza-adenosine (Tubercidin). Biochim Biophys Acta. 1967 Mar 29;138(1):10-25. [Content Brief]

[2]. Bergstrom DE, et al. Antiviral activity of C-5 substituted tubercidin analogues. J Med Chem. 1984 Mar;27(3):285-92. [Content Brief]

[3]. Kouni MH, et al. Prevention of tubercidin host toxicity by nitrobenzylthioinosine 5'-monophosphate for the treatment of schistosomiasis. Antimicrob Agents Chemother. 1989 Jun;33(6):824-7. [Content Brief]

Tubercidin 相关分类

Infection

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7559 mL	18.7793 mL	37.5587 mL	93.8967 mL
	5 mM	0.7512 mL	3.7559 mL	7.5117 mL	18.7793 mL
	10 mM	0.3756 mL	1.8779 mL	3.7559 mL	9.3897 mL
	15 mM	0.2504 mL	1.2520 mL	2.5039 mL	6.2598 mL
	20 mM	0.1878 mL	0.9390 mL	1.8779 mL	4.6948 mL
	25 mM	0.1502 mL	0.7512 mL	1.5023 mL	3.7559 mL
	30 mM	0.1252 mL	0.6260 mL	1.2520 mL	3.1299 mL
	40 mM	0.0939 mL	0.4695 mL	0.9390 mL	2.3474 mL
	50 mM	0.0751 mL	0.3756 mL	0.7512 mL	1.8779 mL
	60 mM	0.0626 mL	0.3130 mL	0.6260 mL	1.5649 mL
	80 mM	0.0469 mL	0.2347 mL	0.4695 mL	1.1737 mL
	100 mM	0.0376 mL	0.1878 mL	0.3756 mL	0.9390 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tubercidin69-33-07-Deazaadenosine杀结核菌素结核菌素BacterialDNA/RNA SynthesisInfluenza VirusAntibioticInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Tubercidin (Synonyms: 结核菌素; 7-Deazaadenosine)

Customer Review

MCE 顾客使用本产品发表的 5 篇科研文献