2. 多肽产品
  3. 多肽及其衍生物
  4. 抑制剂与底物肽
  5. 酶底物
  6. Histone H1-derived Peptide

Histone H1-derived Peptide 

目录号: HY-P2480 纯度: 99.00%
COA 产品使用指南

摩尔计算器

Histone H1-derived Peptide 是一种磷酸肽，肽底物包含 CDK 最佳识别基序的序列，可用于用于检测 CDK1-cyclinB1 的酶活性。

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Custom Peptide Synthesis

Histone H1-derived Peptide Chemical Structure

Histone H1-derived Peptide Chemical Structure

CAS No. : 889112-06-5

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥2200
In-stock
5 mg ￥4800
In-stock
10 mg 现货 询价
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Histone H1-derived Peptide 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Histone H1-derived Peptide is a phosphopeptide and the peptide substrates containes a sequence in accordance with the optimal recognition motif for CDK, can be used to detecting CDK1-cyclinB1 enzyme activity[1][2].

体外研究
(In Vitro)

Histone H1-derived Peptide 用于检测 CDK1-cyclinB1 的酶活性[1]
(1) 将 CDK1-cyclinB1 复合物添加到反应体系中，使其达到 10 nM 的终浓度。
(2) 添加 Histone H1-derived Peptide 底物，使其达到 100 μM 的终浓度。
(3) 加入 200 μM 的 ATP。
(4) 添加 20 mM Tris 缓冲液、50 mM NaCl、0.5 mM DTT 和 0.04% BSA，确保所有成分都在合适的体积比例下混合均匀，调整 PH 为 7。
(5) 添加 2 mM 的 MgCl2。
(6) 进行持续 ATP 耦合测定，利用 ADP 生成磷酸化反应，通过消耗磷酸烯醇式丙酮酸 (PEP) 和 NADH，由丙酮酸激酶/乳酸脱氢酶 (PK/LDH) 催化将其转换回 ATP。监测 NADH 的荧光减少情况，激发波长为 340 nm，发射波长为 460 nm。
(7) 使用线性回归分析处理反应时间曲线，以获取反应速度。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Histone H1-derived Peptide 相关抗体:
分子量

1252.51

Formula

C56H101N17O15
CAS 号
性状

固体

颜色

White to off-white

Sequence

Gly-Gly-Gly-Pro-Ala-Thr-Pro-Lys-Lys-Ala-Lys-Lys-Leu
Sequence Shortening

GGGPATPKKAKKL
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
细胞实验： 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
纯度 & 产品资料
参考文献

Histone H1-derived Peptide 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Histone H1-derived Peptide889112-06-5OthersPeptideBinding affinityInter-domain dynamicsInhibitorinhibitorinhibit

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产品名称：
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目录号：
HY-P2480
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