2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK
  3. EGFR
  4. Tephrosin

Tephrosin  (Synonyms: 灰叶草素; Deguelinol I; Hydroxydeguelin)

目录号: HY-N1166 纯度: 98.02%
Data Sheet SDS COA 产品使用指南 技术支持

溶解度

动物溶解方案

Tephrosin 是一种天然类胡萝卜素，具有较强的抗肿瘤活性。Tephrosin 通过诱导受体内化来诱导 EGFR 和 ErbB2 的降解。

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Tephrosin Chemical Structure

Tephrosin Chemical Structure

CAS No. : 76-80-2

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1 mg ￥3500
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Customer Review

Other Forms of Tephrosin:

Top Publications Citing Use of Products

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Tephrosin is a natural rotenoid which has potent antitumor activities. Tephrosin induces degradation of of EGFR and ErbB2 by inducing internalization of the receptors[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
697 IC50
5.4 μM
Compound: 9
Cytotoxicity against human 697 cells after 48 hrs by MTS assay
Cytotoxicity against human 697 cells after 48 hrs by MTS assay
[PMID: 23895019]
697 IC50
9 μM
Compound: 9
Cytotoxicity against human 697 cells after 72 hrs by MTS assay
Cytotoxicity against human 697 cells after 72 hrs by MTS assay
[PMID: 23895019]
B16 IC50
1.35 μM
Compound: 9
Cytotoxicity against mouse B16 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against mouse B16 cells assessed as reduction in cell viability by MTT assay
[PMID: 28665590]
HCT-116 IC50
0.04 ug
Compound: 7
Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting
Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting
[PMID: 21452840]
HeLa IC50
0.11 μM
Compound: 1
Inhibition of TNF-alpha-induced NF-kappaB activation in human HeLa cells by SEAP reporter gene assay
Inhibition of TNF-alpha-induced NF-kappaB activation in human HeLa cells by SEAP reporter gene assay
[PMID: 18841906]
HepG2 IC50
1.35 μM
Compound: 9
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay
[PMID: 28665590]
HT-29 IC50
> 10 μM
Compound: 9
Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay
Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay
[PMID: 23895019]
Lewis lung carcinoma cell line IC50
1.35 μM
Compound: 9
Cytotoxicity against mouse LL/2 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against mouse LL/2 cells assessed as reduction in cell viability by MTT assay
[PMID: 28665590]
Raji IC50
> 10 μM
Compound: 9
Cytotoxicity against human Raji cells after 48 hrs by MTS assay
Cytotoxicity against human Raji cells after 48 hrs by MTS assay
[PMID: 23895019]
Raji IC50
> 10 μM
Compound: 9
Cytotoxicity against human Raji cells after 72 hrs by MTS assay
Cytotoxicity against human Raji cells after 72 hrs by MTS assay
[PMID: 23895019]
RAW264.7 IC50
2 μM
Compound: 1
Inhibition of LPS-induced NF-kappaB activation in mouse RAW264.7 cells by SEAP reporter gene assay
Inhibition of LPS-induced NF-kappaB activation in mouse RAW264.7 cells by SEAP reporter gene assay
[PMID: 18841906]
U-937 IC50
23 μM
Compound: 2, tephrosin
Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
[PMID: 17158054]
U-937 IC50
30 μM
Compound: 2, tephrosin
Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
[PMID: 17158054]
U-937 IC50
7.3 μM
Compound: 2, tephrosin
Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
[PMID: 17158054]
体外研究
(In Vitro)

Treatment of HT-29 cells with tephrosin (0-10μM) inhibits both the ligand-induced and constitutive phosphorylation of EGFR, ErbB2 and ErbB3, and concomitantly suppresses the activation of the downstream signaling molecules such as Akt and Erk1/2 mitogen-activated protein kinase (MAPK) [1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

410.42

Formula

C23H22O7
CAS 号
性状

固体

颜色

White to off-white

中文名称

灰叶草素
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (243.65 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4365 mL 12.1826 mL 24.3653 mL
5 mM 0.4873 mL 2.4365 mL 4.8731 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.09 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.09 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

扫码获得
动物溶解方案

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， PEG300/PEG400，Tween 80，均可在 MCE 网站选购。
纯度 & 产品资料

纯度： 98.02%

Data Sheet (626 KB) SDS (459 KB)

COA (290 KB)HNMR (258 KB)LCMS (86 KB)

产品使用指南 (1538 KB)
参考文献

Tephrosin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4365 mL 12.1826 mL 24.3653 mL 60.9132 mL
5 mM 0.4873 mL 2.4365 mL 4.8731 mL 12.1826 mL
10 mM 0.2437 mL 1.2183 mL 2.4365 mL 6.0913 mL
15 mM 0.1624 mL 0.8122 mL 1.6244 mL 4.0609 mL
20 mM 0.1218 mL 0.6091 mL 1.2183 mL 3.0457 mL
25 mM 0.0975 mL 0.4873 mL 0.9746 mL 2.4365 mL
30 mM 0.0812 mL 0.4061 mL 0.8122 mL 2.0304 mL
40 mM 0.0609 mL 0.3046 mL 0.6091 mL 1.5228 mL
50 mM 0.0487 mL 0.2437 mL 0.4873 mL 1.2183 mL
60 mM 0.0406 mL 0.2030 mL 0.4061 mL 1.0152 mL
80 mM 0.0305 mL 0.1523 mL 0.3046 mL 0.7614 mL
100 mM 0.0244 mL 0.1218 mL 0.2437 mL 0.6091 mL

Keywords:

Tephrosin76-80-2Deguelinol I Hydroxydeguelin灰叶草素EGFREpidermal growth factor receptorErbB-1HER1Inhibitorinhibitorinhibit

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目录号：
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