2. Cell Cycle/DNA Damage Autophagy Apoptosis
  3. DNA/RNA Synthesis Nucleoside Antimetabolite/Analog Autophagy Apoptosis
  4. Gemcitabine hydrochloride

Gemcitabine hydrochloride  (Synonyms: 盐酸吉西他滨; LY 188011 hydrochloride)

目录号: HY-B0003 纯度: 99.89%
COA 产品使用指南

摩尔计算器

Gemcitabine Hydrochloride (LY 188011 Hydrochloride) 是一种嘧啶核苷类似物抗代谢药 (nucleoside antimetabolite/analog) 和抗肿瘤剂。Gemcitabine 抑制 DNA 合成 (DNA synthesis) 和修复，导致细胞自噬 (autophagy) 和凋亡 (apoptosis)。

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Gemcitabine hydrochloride Chemical Structure

Gemcitabine hydrochloride Chemical Structure

CAS No. : 122111-03-9

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥792
In-stock
100 mg ￥720
In-stock
200 mg ￥1080
In-stock
500 mg ￥1980
In-stock
1 g ￥3100
In-stock
5 g   询价  
10 g   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Gemcitabine hydrochloride:

Gemcitabine hydrochloride 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 151 篇科研文献

WB
Proliferation Assay

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2019 May;392(5):615-622.  [Abstract]

    The expression level of p-JAK2, p-STAT3, Bcl-XL, and Mcl-1 in PANC-1 cells is detected by western blot analysis and is all down-regulated in the treatment groups.

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: Chem Biol Interact. 2018 Jun 25;290:44-51.  [Abstract]

    U2OS and MG-63 cells are treated with Gemcitabine, Licoricidin, or Gemcitabine+Licoricidin for 24 h, followed by the determination of active caspse-3 protein level using western blot. Cells without treatment are used as Control.

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: J Biol Chem. 2017 Jun 2;292(22):9136-9149.  [Abstract]

    The plate clone formation of SW480 and SW620 cells with Gemcitabine (8 nM in SW480 and 16 nM in SW620) and/or PX-12 (4 μM in SW480 and 8 μM in SW620).

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Gemcitabine Hydrochloride (LY 188011 Hydrochloride) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine Hydrochloride inhibits DNA synthesis and repair, resulting in autophagyand apoptosis[1][2].

    IC50 & Target

    DNA synthesis[1]
    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A2780 IC50
    1.25 μM
    Compound: GEM
    Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 33992863]
    A2780 IC50
    1.38 μM
    Compound: GEM
    Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    		[PMID: 33992863]
    A549 IC50
    14.65 μM
    Compound: GEM
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    		[PMID: 33992863]
    A549 IC50
    2.31 μM
    Compound: GEM
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 33992863]
    C2D cell line IC50
    11.9 nM
    Compound: Gemzar
    Inhibitory concentration to inhibit growth of C2D prostate cancer cell line by using MTT assay
    Inhibitory concentration to inhibit growth of C2D prostate cancer cell line by using MTT assay
    		[PMID: 14971910]
    C2G cell line IC50
    3.7 nM
    Compound: Gemzar
    Inhibitory concentration to inhibit growth of C2G prostate cancer cell line by using MTT assay
    Inhibitory concentration to inhibit growth of C2G prostate cancer cell line by using MTT assay
    		[PMID: 14971910]
    L02 IC50
    11.87 μM
    Compound: GEM
    Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 33992863]
    L02 IC50
    4.51 μM
    Compound: GEM
    Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    		[PMID: 33992863]
    MCF7 IC50
    6.35 μM
    Compound: GEM
    Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 33992863]
    NCI-H460 IC50
    2.13 nM
    Compound: Gemcitabine HCl
    Cytotoxicity against human NCI-H460 cells by MTT assay
    Cytotoxicity against human NCI-H460 cells by MTT assay
    		[PMID: 19345581]
    NCI-H460 IC50
    2.13 μM
    Compound: GemcitabineHCl
    Cytotoxicity against human NCI-H460 cells after 3 days by MTT assay
    Cytotoxicity against human NCI-H460 cells after 3 days by MTT assay
    		[PMID: 20356655]
    TRAMP-C1A IC50
    9 nM
    Compound: Gemzar
    Inhibitory concentration to inhibit growth of C1A prostate cancer cell line by using MTT assay
    Inhibitory concentration to inhibit growth of C1A prostate cancer cell line by using MTT assay
    		[PMID: 14971910]
    TRAMP-C2H IC50
    4.7 nM
    Compound: Gemzar
    Inhibitory concentration to inhibit growth of C2H prostate cancer cell line by using MTT assay
    Inhibitory concentration to inhibit growth of C2H prostate cancer cell line by using MTT assay
    		[PMID: 14971910]
    Vero C1008 CC50
    23.22 μM
    Compound: Gemcitabine Hydrochloride
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    Vero C1008 CC50
    23.22 μM
    Compound: Gemcitabine Hydrochloride
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    体外研究
    (In Vitro)

    Gemcitabine Hydrochloride (购自 MedChem Express，0.003-1 μM；3 天) 可有效、强效地杀死小鼠和人类衰老细胞[4]。
    Gemcitabine Hydrochloride 抑制 BxPC-3、Mia Paca-2、PANC-1、PL-45 和 AsPC-1 细胞的生长，IC50分别为 37.6、42.9、92.7、89.3 和 131.4 nM[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Gemcitabine hydrochloride 相关抗体:

    Cell Viability Assay[4]

    Cell Line: Non-senescent and replication-induced senescent new born dermal fibroblasts (NBFs)
    Concentration: 0.003, 0.01, 0.03, 0.1, 0.3, 1 μM
    Incubation Time: 3 days
    Result: Killed replication-induced senescent NBFs for 3 days with 11.0% cell viability.
    体内研究
    (In Vivo)

    Gemcitabine Hydrochloride 可通过气管内喷雾剂给药于大鼠，且无明显毒性，最大耐受剂量为每周一次 4 mg/kg，连续 9 周。Gemcitabine Hydrochloride 通过肺部给药的毒性低于口服给药，剂量为 2、4 和 6 mg/kg[2]。
    使用 Gemcitabine Hydrochloride (50 mg/kg，i.p.) 或 DMAPT/Gemcitabine Hydrochloride l 联合治疗 LSL-KrasG12D/+、LSL-Trp53R172H 和 Pdx-1-Cre 小鼠可显著延长中位生存期 30 多天，而安慰剂组则分别为 254.5 天、255 天和 217.5 天[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    299.66

    Formula

    C9H12ClF2N3O4
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    盐酸吉西他滨；吉西他宾盐酸盐
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 62.5 mg/mL (208.57 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 25 mg/mL (83.43 mM; 超声助溶)

    DMF 中的溶解度 : 2.5 mg/mL (8.34 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.3371 mL 16.6856 mL 33.3712 mL
    5 mM 0.6674 mL 3.3371 mL 6.6742 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.94 mM); 澄清溶液

      此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.94 mM); 澄清溶液

      此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 25 mg/mL (83.43 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.93%

    COA (197 KB) LCMS (472 KB) 元素分析报告 (214 KB)

    产品使用指南 (1538 KB)
    参考文献

    Gemcitabine hydrochloride 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMF / H2O / DMSO 1 mM 3.3371 mL 16.6856 mL 33.3712 mL 83.4279 mL
    5 mM 0.6674 mL 3.3371 mL 6.6742 mL 16.6856 mL
    H2O / DMSO 10 mM 0.3337 mL 1.6686 mL 3.3371 mL 8.3428 mL
    15 mM 0.2225 mL 1.1124 mL 2.2247 mL 5.5619 mL
    20 mM 0.1669 mL 0.8343 mL 1.6686 mL 4.1714 mL
    25 mM 0.1335 mL 0.6674 mL 1.3348 mL 3.3371 mL
    30 mM 0.1112 mL 0.5562 mL 1.1124 mL 2.7809 mL
    40 mM 0.0834 mL 0.4171 mL 0.8343 mL 2.0857 mL
    50 mM 0.0667 mL 0.3337 mL 0.6674 mL 1.6686 mL
    60 mM 0.0556 mL 0.2781 mL 0.5562 mL 1.3905 mL
    80 mM 0.0417 mL 0.2086 mL 0.4171 mL 1.0428 mL
    DMSO 100 mM 0.0334 mL 0.1669 mL 0.3337 mL 0.8343 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Gemcitabine122111-03-9LY 188011盐酸吉西他滨吉西他宾盐酸盐LY188011LY-188011DNA/RNA SynthesisNucleoside Antimetabolite/AnalogAutophagyApoptosisInhibitorinhibitorinhibit

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