Isosteviol (Synonyms: 异甜菊醇; (-)-Isosteviol; iso-Steviol)

目录号: HY-N0872 纯度: ≥98.0%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Isosteviol ((-)-Isosteviol) 是一种甜菊糖苷衍生物，是甜菊糖苷通过酸催化水解产生的。Isosteviol 抑制 DNA 聚合酶和 DNA 拓扑异构酶，并具有抗菌，抗癌和抗结核作用。

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Isosteviol Chemical Structure

CAS No. : 27975-19-5

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥550	In-stock
50 mg	￥500	In-stock
100 mg	￥850	In-stock
200 mg		询价
500 mg		询价

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Isosteviol (Standard) 询价

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生物活性
纯度 & 产品资料
参考文献

生物活性

Isosteviol ((-)-Isosteviol) is a derivative of Stevioside through acid catalyzed hydrolysis of Stevioside. Isosteviol inhibits DNA polymerase and DNA topoisomerase and has antibacterial, anticancer and anti-tuberculosis effects^[1]^[2]^[3]^[4].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 50 μM Compound: 1, Isosteviol	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 23693151]
A549	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 23347685]
B16-F10	IC₅₀	> 100 μM Compound: 1, Isosteviol	Cytotoxicity against mouse B16F10 cells after 48 hrs by MTT assay Cytotoxicity against mouse B16F10 cells after 48 hrs by MTT assay	[PMID: 24685545]
EC9706	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human EC9706 cells after 72 hrs by MTT assay Antiproliferative activity against human EC9706 cells after 72 hrs by MTT assay	[PMID: 22901386]
ECa-109 cell line	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human ECA109 cells after 72 hrs by MTT assay Antiproliferative activity against human ECA109 cells after 72 hrs by MTT assay	[PMID: 22901386]
HCT-116	IC₅₀	> 100 μM Compound: 1; Isosteviol	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 26994841]
HCT-116	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 22901386]
HCT-116	IC₅₀	> 150 μM Compound: 1	Cytotoxicity against human HCT116 cells assessed as decrease in cell proliferation after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as decrease in cell proliferation after 72 hrs by MTT assay	[PMID: 27777008]
HeLa	IC₅₀	> 50 μM Compound: 1, Isosteviol	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 23693151]
HeLa	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 23347685]
HepG2	IC₅₀	> 100 μM Compound: 1, Isosteviol	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 24685545]
HGC-27	IC₅₀	> 100 μM Compound: 1; Isosteviol	Cytotoxicity against human HGC27 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HGC27 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 26994841]
HL-60	IC₅₀	75 μM Compound: 1, isosteviol	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 26042548]
JeKo-1	IC₅₀	> 100 μM Compound: 1; Isosteviol	Cytotoxicity against human JeKo1 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human JeKo1 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 26994841]
JeKo-1	IC₅₀	> 150 μM Compound: 1	Cytotoxicity against human JeKo1 cells assessed as decrease in cell proliferation after 72 hrs by MTT assay Cytotoxicity against human JeKo1 cells assessed as decrease in cell proliferation after 72 hrs by MTT assay	[PMID: 27777008]
PC-3	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 22901386]
Raji	IC₅₀	> 50 μM Compound: 1, Isosteviol	Antiproliferative activity against human Raji cells after 48 hrs by MTT assay Antiproliferative activity against human Raji cells after 48 hrs by MTT assay	[PMID: 23693151]
Raji	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human Raji cells after 48 hrs by MTT assay Cytotoxicity against human Raji cells after 48 hrs by MTT assay	[PMID: 23347685]
Raji	IC₅₀	321 μM Compound: 7	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique	[PMID: 19131254]
SGC-7901	IC₅₀	> 50 μM Compound: 1, Isosteviol	Antiproliferative activity against human SGC7901 cells after 48 hrs by MTT assay Antiproliferative activity against human SGC7901 cells after 48 hrs by MTT assay	[PMID: 23693151]
SGC-7901	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay	[PMID: 23347685]

体外研究
(In Vitro)

Isosteviol ((-)-Isosteviol) dose-dependently relaxed the vasopressin (10^-8 M)-induced vasoconstriction in isolated aortic rings with or without endothelium. However, in the presence of potassium chloride (3×10^-2 M), the vasodilator effect of isosteviol on arterial strips disappeared. Only the inhibitors specific for the ATP-sensitive potassium (KATP) channel or small conductance calcium-activated potassium (SKCa) channel inhibited the vasodilator effect of isosteviol in isolated aortic rings contracted with 10^-8 M vasopressin^[1].
The attenuation by isosteviol of the vasopressin- and phenylephrine-induced increase in [Ca²⁺]i was inhibited by glibenclamide, apamin and 4-aminopyridine but not by charybdotoxin. Furthermore, the inhibitory action of isosteviol on [Ca ²⁺]i was blocked when A7r5 cells co-treated with glibenclamide and apamin in conjunction with 4-aminopyridine were present^[2].
Isosteviol (1-100 micromol/l) inhibits angiotensin-II-induced DNA synthesis and endothelin-1 secretion. Measurements of 2'7'-dichlorofluorescin diacetate, a redox-sensitive fluorescent dye, showed an isosteviol-mediated inhibition of intracellular reactive oxygen species generated by the effects of angiotensin II^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

318.45

Formula

C₂₀H₃₀O₃

CAS 号

27975-19-5

性状

固体

颜色

White to off-white

中文名称

异甜菊醇

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 100 mg/mL (314.02 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.1402 mL	15.7011 mL	31.4021 mL
5 mM	0.6280 mL	3.1402 mL	6.2804 mL
10 mM	0.3140 mL	1.5701 mL	3.1402 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (8.64 mM); 澄清溶液

此方案可获得 ≥ 2.75 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (8.64 mM); 澄清溶液

此方案可获得 ≥ 2.75 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.75 mg/mL (8.64 mM); 澄清溶液

此方案可获得 ≥ 2.75 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: ≥98.0%

选择批次：

Data Sheet (628 KB) SDS (252 KB)

COA (293 KB) HNMR (251 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Wong KL, et al. Isosteviol acts on potassium channels to relax isolated aortic strips of Wistar rat. Life Sci. 2004 Mar 26;74(19):2379-87. [Content Brief]

[2]. Wong KL, et al. Isosteviol as a potassium channel opener to lower intracellular calcium concentrations in cultured aortic smooth muscle cells. Planta Med. 2004 Feb;70(2):108-12. [Content Brief]

[3]. Wong KL, et al. Antiproliferative effect of isosteviol on angiotensin-II-treated rat aortic smooth muscle cells. Pharmacology. 2006;76(4):163-9. [Content Brief]

[4]. Asad Ullah, et al. Bioactivity Profile of the Diterpene Isosteviol and its Derivatives. Molecules. 2019 Feb 14;24(4):678. [Content Brief]

Isosteviol 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1402 mL	15.7011 mL	31.4021 mL	78.5053 mL
	5 mM	0.6280 mL	3.1402 mL	6.2804 mL	15.7011 mL
	10 mM	0.3140 mL	1.5701 mL	3.1402 mL	7.8505 mL
	15 mM	0.2093 mL	1.0467 mL	2.0935 mL	5.2337 mL
	20 mM	0.1570 mL	0.7851 mL	1.5701 mL	3.9253 mL
	25 mM	0.1256 mL	0.6280 mL	1.2561 mL	3.1402 mL
	30 mM	0.1047 mL	0.5234 mL	1.0467 mL	2.6168 mL
	40 mM	0.0785 mL	0.3925 mL	0.7851 mL	1.9626 mL
	50 mM	0.0628 mL	0.3140 mL	0.6280 mL	1.5701 mL
	60 mM	0.0523 mL	0.2617 mL	0.5234 mL	1.3084 mL
	80 mM	0.0393 mL	0.1963 mL	0.3925 mL	0.9813 mL
	100 mM	0.0314 mL	0.1570 mL	0.3140 mL	0.7851 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isosteviol27975-19-5(-)-Isosteviol iso-Steviol异甜菊醇Interleukin RelatedReactive Oxygen SpeciesTopoisomeraseILSteviosideDNApolymerasetopoisomeraseantibacterialanticanceranti-tuberculosisInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Isosteviol (Synonyms: 异甜菊醇; (-)-Isosteviol; iso-Steviol)

Customer Review

Other Forms of Isosteviol: