Lycorine (Synonyms: 石蒜碱)

目录号: HY-N0288 纯度: 99.87%: FAQs
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摩尔计算器

Lycorine 是从石蒜科植物中提取的天然生物碱。Lycorine 是强效具有口服活性的 SCAP 抑制剂，K_d 值 15.24 nM。Lycorine 下调 SCAP 蛋白水平而不改变其转录。Lycorine 也是黑色素瘤血管生成抑制剂。Lycorine 有潜力用于前列腺癌和代谢疾病的研究。

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Lycorine Chemical Structure

CAS No. : 476-28-8

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥880	In-stock
50 mg	￥800	In-stock
100 mg	￥1300	In-stock
200 mg		询价
500 mg		询价

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Other Forms of Lycorine:

Lycorine hydrochloride monohydrate In-stock
Lycorine hydrochloride In-stock

MCE 顾客使用本产品发表的 13 篇科研文献

•Research Square Preprint. 2022 Jan.

Lycorine 相关产品

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生物活性
纯度 & 产品资料
参考文献

生物活性

Lycorine is a natural alkaloid extracted from the Amaryllidaceae plant. Lycorine is a potent and orally active SCAP inhibitor with a K_d value 15.24 nM. Lycorine downregulates the SCAP protein level without changing its transcription^[2]. Lycorine is also a melanoma vasculogenic inhibitor^[3]. Lycorine can be used for the study of prostate cancer and metabolic diseases^[2].

IC₅₀ & Target

Kd: 15.24 nM (SCAP)^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human A2780 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human A2780 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
A-431	ED₅₀	1.3 μg/mL Compound: 2	Cytotoxicity against human A431 cells after 3 days by sulforhodamine B assay Cytotoxicity against human A431 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
A549	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human A549 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human A549 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
A549	GI₅₀	0.9 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-resistant human A549 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-resistant human A549 cells after 72 hrs by MTT assay	[PMID: 22019045]
A549	IC₅₀	1.9 μM Compound: 14	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 23190013]
A549	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-resistant human A549 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-resistant human A549 cells after 72 hrs by MTT assay	[PMID: 20415482]
A549	GI₅₀	3 μM Compound: 1	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 24393582]
A549	IC₅₀	4.2 μM Compound: 1	Cytotoxicity against human A549 cells after 72 hrs by MTT colorimetric assay Cytotoxicity against human A549 cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
A549	IC₅₀	4.3 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-resistant human A549 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-resistant human A549 cells after 72 hrs by MTT assay	[PMID: 19788245]
AGS	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human AGS cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human AGS cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
B16-F10	GI₅₀	2 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-sensitive mouse B16F10 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 22019045]
B16-F10	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-sensitive mouse B16F10 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 20415482]
B16-F10	GI₅₀	2.6 μM Compound: 1	Growth inhibition of mouse B16F10 cells by MTT assay Growth inhibition of mouse B16F10 cells by MTT assay	[PMID: 24393582]
B16-F10	IC₅₀	6.3 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-sensitive mouse B16F10 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 19788245]
B16-F10	IC₅₀	6.3 μM Compound: 1	Cytotoxicity against mouse B16F10 cells after 72 hrs by MTT colorimetric assay Cytotoxicity against mouse B16F10 cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
BEAS-2B	IC₅₀	4.9 μM Compound: 14	Cytotoxicity against human BEAS2B cells after 48 hrs by MTT assay Cytotoxicity against human BEAS2B cells after 48 hrs by MTT assay	[PMID: 23190013]
BHK-21	CC₅₀	4.37 μM Compound: 8	cytotoxicity against golden hamster BHK-21 cells incubated for 72 hrs by MTT assay cytotoxicity against golden hamster BHK-21 cells incubated for 72 hrs by MTT assay	[PMID: 32845145]
BJ	IC₅₀	1.9 μM Compound: 2	Cytotoxicity against human BJ cells after 72 hrs by Calcein AM assay Cytotoxicity against human BJ cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
BT-549	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human BT-549 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human BT-549 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
CCRF-CEM	IC₅₀	1.6 μM Compound: 2	Cytotoxicity against human CEM cells after 72 hrs by Calcein AM assay Cytotoxicity against human CEM cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
COLO 201	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human COLO201 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human COLO201 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
G-361	IC₅₀	5 μM Compound: 2	Cytotoxicity against human G361 cells after 72 hrs by Calcein AM assay Cytotoxicity against human G361 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
HeLa	IC₅₀	10.6 μM Compound: 2	Cytotoxicity against human HeLa cells after 72 hrs by Calcein AM assay Cytotoxicity against human HeLa cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
HepG2	IC₅₀	3.7 μM Compound: 14	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 23190013]
HL-60	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
HL-60	IC₅₀	1.6 μM Compound: 14	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 23190013]
Hs 683	GI₅₀	0.9 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-sensitive human Hs683 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-sensitive human Hs683 cells after 72 hrs by MTT assay	[PMID: 22019045]
Hs 683	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-sensitive human Hs 683 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-sensitive human Hs 683 cells after 72 hrs by MTT assay	[PMID: 20415482]
Hs 683	GI₅₀	2.1 μM Compound: 1	Growth inhibition of human Hs683 cells by MTT assay Growth inhibition of human Hs683 cells by MTT assay	[PMID: 24393582]
Hs 683	IC₅₀	6.7 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-sensitive human Hs683 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-sensitive human Hs683 cells after 72 hrs by MTT assay	[PMID: 19788245]
Hs 683	IC₅₀	6.9 μM Compound: 1	Cytotoxicity against human Hs683 cells after 72 hrs by MTT colorimetric assay Cytotoxicity against human Hs683 cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
HT-1080	ED₅₀	0.5 μg/mL Compound: 2	Cytotoxicity against human HT1080 cells after 3 days by sulforhodamine B assay Cytotoxicity against human HT1080 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
HT-29	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human HT-29 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human HT-29 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
HT-29	IC₅₀	3.2 μM Compound: 14	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 23190013]
Jurkat	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human Jurkat cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human Jurkat cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
K562	IC₅₀	2.3 μM Compound: 14	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 23190013]
K562	IC₅₀	3.6 μM Compound: 2	Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
KB	ED₅₀	0.3 μg/mL Compound: 2	Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
KB-V1	ED₅₀	0.4 μg/mL Compound: 2	Cytotoxicity against human KBV1 cells after 3 days by sulforhodamine B assay Cytotoxicity against human KBV1 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
LLC-MK2	CC₅₀	3.81 μM Compound: 8	Cytotoxicity against Rhesus macaque LLC-MK2 cells incubated for 72 hrs by MTT assay Cytotoxicity against Rhesus macaque LLC-MK2 cells incubated for 72 hrs by MTT assay	[PMID: 32845145]
LNCaP	ED₅₀	0.5 μg/mL Compound: 2	Cytotoxicity against human LNCAP cells after 3 days by sulforhodamine B assay Cytotoxicity against human LNCAP cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
Macrophage	IC₅₀	2.5 μM Compound: 9	Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay	[PMID: 15270564]
MCF7	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
MCF7	GI₅₀	10.9 μM Compound: 1	Growth inhibition of human MCF7 cells by MTT assay Growth inhibition of human MCF7 cells by MTT assay	[PMID: 24393582]
MCF7	IC₅₀	13 μM Compound: 2	Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
MCF7	GI₅₀	4 μM Compound: 1	Growth inhibition of human MCF7 cells after 72 hrs by MTT assay Growth inhibition of human MCF7 cells after 72 hrs by MTT assay	[PMID: 22019045]
MDA-MB-231	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
MOLT-4	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
NCI-H1299	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human NCI-H1299 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human NCI-H1299 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
NHDF	IC₅₀	> 100 μM Compound: 1, lycorine	Growth inhibition of human NHDF after 72 hrs by MTT assay Growth inhibition of human NHDF after 72 hrs by MTT assay	[PMID: 19788245]
NHDF	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human NHDF cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human NHDF cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
P388	ED₅₀	0.9 μg/mL Compound: 2	Cytotoxicity against mouse P388 cells after 3 days by sulforhodamine B assay Cytotoxicity against mouse P388 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
PANC-1	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human PANC-1 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human PANC-1 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
PC-3	GI₅₀	4 μM Compound: 1	Growth inhibition of human PC3 cells after 72 hrs by MTT assay Growth inhibition of human PC3 cells after 72 hrs by MTT assay	[PMID: 22019045]
SAOS-2	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human SAOS-2 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human SAOS-2 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
SK-BR-3	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human SK-BR-3 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human SK-BR-3 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
SK-MEL	IC₅₀	8.4 μM Compound: 1	Cytotoxicity against human SK-MEL cells after 72 hrs by MTT colorimetric assay Cytotoxicity against human SK-MEL cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
SK-MEL-2	ED₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against human SK-MEL-2 cells after 3 days by sulforhodamine B assay Cytotoxicity against human SK-MEL-2 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
SK-MEL-28	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-resistant human SK-MEL-28 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 20415482]
SK-MEL-28	GI₅₀	3.7 μM Compound: 1	Growth inhibition of human SK-MEL-28 cells by MTT assay Growth inhibition of human SK-MEL-28 cells by MTT assay	[PMID: 24393582]
SK-MEL-28	GI₅₀	4 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-resistant human SK-MEL-28 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 22019045]
SK-MEL-28	IC₅₀	8.5 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-resistant human SK-MEL-28 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 19788245]
SW480	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human SW480 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human SW480 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
T98G	GI₅₀	3 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-resistant human T98G cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-resistant human T98G cells after 72 hrs by MTT assay	[PMID: 22019045]
T98G	GI₅₀	5.2 μM Compound: 1	Growth inhibition of human T98G cells by MTT assay Growth inhibition of human T98G cells by MTT assay	[PMID: 24393582]
U-373MG ATCC	ED₅₀	0.3 μg/mL Compound: 2	Cytotoxicity against human U373 cells after 3 days by sulforhodamine B assay Cytotoxicity against human U373 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
U-373MG ATCC	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-resistant human U373 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-resistant human U373 cells after 72 hrs by MTT assay	[PMID: 20415482]
U-373MG ATCC	GI₅₀	3 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-resistant human U373 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-resistant human U373 cells after 72 hrs by MTT assay	[PMID: 22019045]
U-373MG ATCC	IC₅₀	7.6 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-resistant human U373 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-resistant human U373 cells after 72 hrs by MTT assay	[PMID: 19788245]
U-373MG ATCC	IC₅₀	7.6 μM Compound: 1	Cytotoxicity against human U373 cells after 72 hrs by MTT colorimetric assay Cytotoxicity against human U373 cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
Vero	IC₅₀	0.24 μg/mL Compound: 16	Antiviral activity against Dengue virus type 4 infected in african green monkey Vero cells after 6 days by plaque reduction assay Antiviral activity against Dengue virus type 4 infected in african green monkey Vero cells after 6 days by plaque reduction assay	[PMID: 1336040]
Vero	IC₅₀	0.28 μg/mL Compound: 16	Antiviral activity against Yellow fever virus Ashibi infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against Yellow fever virus Ashibi infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 1336040]
Vero	IC₅₀	0.33 μg/mL Compound: 16	Antiviral activity against Japanese encephalitis virus Nakayama infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against Japanese encephalitis virus Nakayama infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 1336040]
Vero	IC₅₀	0.5 μg/mL Compound: 16	Antiviral activity against Punta Toro virus Adames infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against Punta Toro virus Adames infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 1336040]
Vero	IC₅₀	0.93 μg/mL Compound: 16	Antiviral activity against Rift Valley fever virus infected in african green monkey Vero cells after 6 days by plaque reduction assay Antiviral activity against Rift Valley fever virus infected in african green monkey Vero cells after 6 days by plaque reduction assay	[PMID: 1336040]
Vero C1008	CC₅₀	14.9 μM Compound: 8	Cytotoxicity against African green monkey Vero E6 cells incubated for 72 hrs by MTS assay Cytotoxicity against African green monkey Vero E6 cells incubated for 72 hrs by MTS assay	[PMID: 32845145]
WI-38	IC₅₀	> 100 μM Compound: 1, lycorine	Growth inhibition of human WI38 cells after 72 hrs by MTT assay Growth inhibition of human WI38 cells after 72 hrs by MTT assay	[PMID: 19788245]
ZR-75-1	ED₅₀	0.9 μg/mL Compound: 2	Cytotoxicity against human ZR-75-1 cells after 3 days by sulforhodamine B assay Cytotoxicity against human ZR-75-1 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]

体外研究
(In Vitro)

Lycorine 对上述 4 种 PCa 细胞系具有剂量依赖性抑制细胞增殖作用，IC₅₀范围为 5 μM~10 μM，也说明 Lycorine 对 PNT1A 细胞增殖的影响较小^[1]。
SCAP (SREBF 分子伴侣) 是一种内质网到高尔基体的转运蛋白，会发生构象变化，通过与 INSIG1 (胰岛素诱导基因) 形成复合物来调节/保护内质网中的 SREBF 1)^[2]。
Lycorine (5-40 μM；16 小时) 以剂量依赖性方式显著抑制 SREBF 活性 (高达-70%)，并且不会在细胞中引起明显的细胞毒性^[2]。
Lycorine (10 -20 μM；2-16 小时) 剂量和时间依赖性地降低 HL-7702 细胞中成熟的 SREBF1 和 SREBF2 蛋白^[2]。
Lycorine (20 μM；16 小时) 既不激活 NR1H3 转录也不影响 NR1H3 靶基因，包括 ABCG5 和 ABCG8，但甾醇激活 NR1H3 转录活性^[1]。
Lycorine (0-25 μM；48 小时) 处理以剂量依赖性方式显著抑制血管内皮 (VE)-钙粘蛋白的表达，并略微降低 C8161 细胞中 Sema4D 的表达。然而，其他 6 个基因的表达不受影响^[3]。
Lycorine (0-25 μM；48小时) 处理显著降低了 C8161 细胞中的 VE-cadherin 蛋白水平^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HL-7702/SRE-Luc cells
Concentration:	16 hours
Incubation Time:	5 μM; 10 μM; 20 μM; 40 μM
Result:	Had no cytotoxicity on HL-7702 cells.

Western Blot Analysis^[2]

Cell Line:	HL-7702/SRE-Luc cells
Concentration:	2 hours, 4 hours, 8 hours, 12 hours, 16 hours
Incubation Time:	10 μM; 20 μM
Result:	Decreased p-SREBF1, m-SREBF1, p-SREBF2 and p-SREBF1 protein expression.

RT-PCR^[3]

Cell Line:	C8161 cells
Concentration:	0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM
Incubation Time:	48 hours
Result:	Markedly suppressed the expression of VE-cadherin in a dose-dependent manner and also slightly diminished the expression of Sema4D in C8161 cells.

体内研究
(In Vivo)

Lycorine (口服；15 mg/kg、30 mg/kg；每日一次) 减轻脂肪堆积和代谢综合征，增加小鼠体内脂肪酸以及前体和成熟 SREBF 的脂肪分解和氧化^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	High-fat diet (HFD)-fed C57BL/6J mice^[2]
Dosage:	15 mg/kg, 30 mg/kg
Administration:	Oral chow; once daily
Result:	Ameliorated high-fat diet-induced hyperlipidemia, hepatic steatosis, and insulin resistance in mice.

分子量

287.31

Formula

C₁₆H₁₇NO₄

CAS 号

476-28-8

性状

固体

颜色

White to off-white

中文名称

石蒜碱

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

细胞实验：

DMSO 中的溶解度 : 25 mg/mL (87.01 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.4806 mL	17.4028 mL	34.8056 mL
5 mM	0.6961 mL	3.4806 mL	6.9611 mL
10 mM	0.3481 mL	1.7403 mL	3.4806 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.70 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.70 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.70 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： 50% PEG300 50% Saline
Solubility: 25 mg/mL (87.01 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.87%

选择批次：

Data Sheet (663 KB) SDS (419 KB)

COA (295 KB) HNMR (257 KB) RP-HPLC (253 KB) LCMS (84 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Hu M, et al.Lycorine is a novel inhibitor of the growth and metastasis of hormone-refractory prostate cancer.Oncotarget. 2015 Jun 20;6(17):15348-61.

[2]. Zu-Guo Zheng, et al. Discovery of a Potent SCAP Degrader That Ameliorates HFD-induced Obesity, Hyperlipidemia and Insulin Resistance via an Autophagy-Independent Lysosomal Pathway. Autophagy. 2020 May 20;1-22. [Content Brief]

[3]. [1].Liu, R. et al. Lycorine hydrochloride inhibits metastatic melanoma cell-dominant vasculogenic mimicry. Pigment cell & melanoma research 25, 630-638, doi:10.1111/j.1755-148X.2012.01036.x (2012). [Content Brief]

Lycorine 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4806 mL	17.4028 mL	34.8056 mL	87.0140 mL
	5 mM	0.6961 mL	3.4806 mL	6.9611 mL	17.4028 mL
	10 mM	0.3481 mL	1.7403 mL	3.4806 mL	8.7014 mL
	15 mM	0.2320 mL	1.1602 mL	2.3204 mL	5.8009 mL
	20 mM	0.1740 mL	0.8701 mL	1.7403 mL	4.3507 mL
	25 mM	0.1392 mL	0.6961 mL	1.3922 mL	3.4806 mL
	30 mM	0.1160 mL	0.5801 mL	1.1602 mL	2.9005 mL
	40 mM	0.0870 mL	0.4351 mL	0.8701 mL	2.1754 mL
	50 mM	0.0696 mL	0.3481 mL	0.6961 mL	1.7403 mL
	60 mM	0.0580 mL	0.2900 mL	0.5801 mL	1.4502 mL
	80 mM	0.0435 mL	0.2175 mL	0.4351 mL	1.0877 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Lycorine (Synonyms: 石蒜碱)

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MCE 顾客使用本产品发表的 13 篇科研文献

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Lycorine (Synonyms: 石蒜碱)

Customer Review

Other Forms of Lycorine:

Lycorine 相关抗体

MCE 顾客使用本产品发表的 13 篇科研文献

Lycorine 相关产品

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