2. MAPK/ERK Pathway Apoptosis Cell Cycle/DNA Damage
  3. Mixed Lineage Kinase RIP kinase CDK
  4. NecroIr2

NecroIr2 是一种铱 (III) 复合物，是顺铂 (Cisplatin, HY-17394) 耐药肺癌细胞 (A549R) 的坏死诱导剂。NecroIr2 选择性积累在线粒体中，导致氧化应激和线粒体膜电位 (MMP) 的损失。NecroIr2 能够激活受体相互作用的丝氨酸苏氨酸激酶 3 (RIPK3) 和混合谱系激酶结构域样假激酶 (MLKL)，调节 CDK4 表达。

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NecroIr2 Chemical Structure

NecroIr2 Chemical Structure

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥6930
In-stock
1 mg ￥1818
In-stock
5 mg ￥4000
In-stock
10 mg 现货 询价
50 mg   询价  
100 mg   询价  

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Customer Review

NecroIr2 相关抗体

Top Publications Citing Use of Products

查看 RIP kinase 亚型特异性产品:

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RIPK1 RIPK2 RIPK3

查看 CDK 亚型特异性产品:

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CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

NecroIr2 is an iridium(III) complex, serves as necroptosis inducers in Cisplatin (HY-17394)-resistant lung cancer cells (A549R). NecroIr2 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr2 activates receptor-interacting serine-threonine kinase 3 (RIPK3) and mixed lineage kinase domain-like pseudokinase (MLKL), and regulates CDK4 expression[1].

IC50 & Target[1]

CDK4

 

RIPK3

 

RIPK1

 

体外研究
(In Vitro)

NecroIr2 (2 μM; 1-2 d) exerts subcellular distribution over 90% accumulated in mitochondria in non-labelled A549R and CFSE labelled L02 cells[1].
NecroIr2 (1.5 μM and 3 μM; 24 h) results ROS generation increasing and loss of mitochondrial membrane potential[1].
NecroIr2 (1.5 μM and 3 μM; 24 h) activates necroptosis proteins, increases the phosphorylation of RIPK1 and RIPK3[1].
NecroIr2 (0.75 μM and 1.5 μM; 24 h) induces necroptosis by arresting cell cycle at G0/G1[1].
NecroIr2 (0.375-1.5 μM; 24 h) inhibits A549R cells proliferation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

NecroIr2 相关抗体:

Western Blot Analysis[1]

Cell Line: A549R cells
Concentration: 1.5 μM and 3 μM
Incubation Time: 24 hours
Result: Increased phospho-RIPK1 (p-PIPK1), total RIPK3, and phospho-RIPK3 (p-PIPK3) level.

Cell Cycle Analysis[1]

Cell Line: A549R cells
Concentration: 0 μM, 0.75 μM and 1.5 μM
Incubation Time: 24 hours
Result: Arrested cell cycle at G0/G1 phase in a dose-dependent manner.

Cell Proliferation Assay[1]

Cell Line: A549R cells
Concentration: 0 μM, 0.375 μM, 0.75 μM and 1.5 μM
Incubation Time: 24 hours
Result: Inhibits cell proliferation in a dose-dependent manner.
分子量

926.44

Formula

C46H30ClIrN6O2-
性状

固体

颜色

Brown to reddish brown

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 50 mg/mL (53.97 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0794 mL 5.3970 mL 10.7940 mL
5 mM 0.2159 mL 1.0794 mL 2.1588 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

NecroIr2 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0794 mL 5.3970 mL 10.7940 mL 26.9850 mL
5 mM 0.2159 mL 1.0794 mL 2.1588 mL 5.3970 mL
10 mM 0.1079 mL 0.5397 mL 1.0794 mL 2.6985 mL
15 mM 0.0720 mL 0.3598 mL 0.7196 mL 1.7990 mL
20 mM 0.0540 mL 0.2699 mL 0.5397 mL 1.3493 mL
25 mM 0.0432 mL 0.2159 mL 0.4318 mL 1.0794 mL
30 mM 0.0360 mL 0.1799 mL 0.3598 mL 0.8995 mL
40 mM 0.0270 mL 0.1349 mL 0.2699 mL 0.6746 mL
50 mM 0.0216 mL 0.1079 mL 0.2159 mL 0.5397 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NecroIr2NecroIr 2NecroIr-2Mixed Lineage KinaseRIP kinaseCDKMLKsReceptor-interacting protein kinasesRIPKCyclin dependent kinaseCisplatinCisplatin-resistant lung cancer cellsmitochondriamitochondrial membrane potentialMMPnecroptosisInhibitorinhibitorinhibit

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产品名称：
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目录号：
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