2. Immunology/Inflammation
  3. NOD-like Receptor (NLR)
  4. Ruscogenin

Ruscogenin  (Synonyms: 鲁斯可皂苷元)

目录号: HY-N0496 纯度: 99.59%
COA 产品使用指南 技术支持

Ruscogenin 是从 Ophiopogon japonicus 提取的一种重要甾体皂苷元，通过抑制 TXNIP/NLRP3 炎症体激活和 MAPK 途径减轻脑缺血诱导的血脑屏障功能障碍，Ruscogenin 具有显著的抗炎和抗血栓活性。Ruscogenin 有口服活性。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Ruscogenin Chemical Structure

Ruscogenin Chemical Structure

CAS No. : 472-11-7

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥805
In-stock
1 mg ￥377
In-stock
5 mg ￥850
In-stock
10 mg ￥1318
In-stock
25 mg ￥2163
In-stock
50 mg ￥3225
In-stock
100 mg ￥4738
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Ruscogenin:

Ruscogenin 相关抗体

Top Publications Citing Use of Products

查看 NOD-like Receptor (NLR) 亚型特异性产品:

查看所有亚型
NLRP3 NLRP1 NOD1 NOD2

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Ruscogenin, an important steroid sapogenin derived from Ophiopogon japonicus, attenuates cerebral ischemia-induced blood-brain barrier dysfunction by suppressing TXNIP/NLRP3 inflammasome activation and the MAPK pathway. Ruscogenin exerts significant anti-inflammatory and anti-thrombotic activities. Ruscogenin has orally bioactivity[1][2].

IC50 & Target

NLRP3

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
BXPC-3 IC50
7.32 μM
Compound: 48
Anticancer activity against human BXPC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Anticancer activity against human BXPC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 36332549]
SW1990 IC50
8.14 μM
Compound: 48
Anticancer activity against human SW1990 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Anticancer activity against human SW1990 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 36332549]
THP-1 IC50
2.27 μM
Compound: 1
Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
[PMID: 20561785]
体外研究
(In Vitro)

Ruscogenin (0.01-40 μM；24 小时) 可提高 OGD/R 处理下 bEnd.3 细胞的细胞活力[1]。
Ruscogenin (0.01-10 μM；24 小时) 在经 OGD/R 处理的 bEnd.3 细胞中可恢复内皮细胞屏障渗漏，抑制 IL-Iβ 和 Caspase-1 的表达，并降低 TXNIP/NLRP3 通路信号[1]。
Ruscogenin（0.01-10 μM； 4 小时）在经 OGD/R 处理的 bEnd.3 细胞中可抑制 ROS 的产生，并降低 MAPK 通路的信号[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Ruscogenin 相关抗体:

Cell Viability Assay[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 24 h
Result: Showed that the cell viability reduction in OGD/R-induced bEnd.3 cell was significantly recovered.

Immunofluorescence[1]

Cell Line: bEnd.3 cell
Concentration: 0.1 μM, 1 μM, 10 μM
Incubation Time: 24 h
Result: Demonstrated increase in the TEER value and inhibition the sodium fluorescein permeability.

Western Blot Analysis[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 24 h
Result: Demonstrated downregulation of the expression of IL-1β and Caspase-1 proteins, and inhibition in the expressions of NLRP3 and TXNIP.

Immunofluorescence[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 4 h
Result: Demonstrated reduction in the production of ROS.
体内研究
(In Vivo)

Ruscogenin (10 mg/kg；灌胃；1次) 改善 MCAO/R 诱导的脑组织损伤，抑制 IL-1β 和 Caspase-1 的表达并抑制TXNIP/NLRP3 的激活[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MCAO/R Mice[1]
Dosage: 10mg/kg
Administration: Oral Gavage (i.g.)
Result: Showed smaller infarct size, ameliorating histopathological damage by decreasing the cell loss, and significant increase in CBF (cerebral blood flow) compared to the model group.
Resulted inhibiting the expression of IL-1β and Caspase-1, NLRP3 and TXNIP compared to the model group.
分子量

430.62

Formula

C27H42O4
CAS 号
性状

固体

颜色

White to off-white

中文名称

鲁斯可皂苷元；螺可吉宁
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

Ethanol 中的溶解度 : 25 mg/mL (58.06 mM; 超声助溶)

DMSO 中的溶解度 : 4.31 mg/mL (10.01 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3222 mL 11.6112 mL 23.2223 mL
5 mM 0.4644 mL 2.3222 mL 4.6445 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： 50% PEG300    50% Saline

    Solubility: 1.67 mg/mL (3.88 mM); 悬浊液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.59%

COA (288 KB) HNMR (263 KB) RP-HPLC (243 KB) LCMS (98 KB)

产品使用指南 (1538 KB)
参考文献

Ruscogenin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO / Ethanol 1 mM 2.3222 mL 11.6112 mL 23.2223 mL 58.0558 mL
5 mM 0.4644 mL 2.3222 mL 4.6445 mL 11.6112 mL
10 mM 0.2322 mL 1.1611 mL 2.3222 mL 5.8056 mL
Ethanol 15 mM 0.1548 mL 0.7741 mL 1.5482 mL 3.8704 mL
20 mM 0.1161 mL 0.5806 mL 1.1611 mL 2.9028 mL
25 mM 0.0929 mL 0.4644 mL 0.9289 mL 2.3222 mL
30 mM 0.0774 mL 0.3870 mL 0.7741 mL 1.9352 mL
40 mM 0.0581 mL 0.2903 mL 0.5806 mL 1.4514 mL
50 mM 0.0464 mL 0.2322 mL 0.4644 mL 1.1611 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ruscogenin472-11-7鲁斯可皂苷元螺可吉宁NOD-like Receptor (NLR)Cardiovascular DiseaseAnti-inflammationInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Ruscogenin
目录号：
HY-N0496
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z