1. Apoptosis Anti-infection Autophagy Neuronal Signaling Membrane Transporter/Ion Channel
  2. Apoptosis Parasite Autophagy Caspase Calcium Channel
  3. Cinchonine

Cinchonine  (Synonyms: 辛可宁; (8R,9S)-Cinchonine; LA40221)

目录号: HY-Y0152 纯度: 99.56%
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Cinchonine 是金鸡纳树皮中的天然化合物,对于疟疾、肿瘤、炎症、血小板凝结和肥胖都具有抑制作用。金鸡纳碱通过激活 Caspase-3 蛋白抑制细胞增殖和自噬 (autophagy),诱导细胞凋亡 (apoptosis)。Cinchonine 可激活内质网应激诱导的人肝癌细胞凋亡。

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Cinchonine Chemical Structure

Cinchonine Chemical Structure

CAS No. : 118-10-5

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10 mM * 1 mL in DMSO ¥220
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1 g ¥200
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5 g ¥420
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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Cinchonine is a natural compound present in Cinchona bark with antimalarial, antitumor, anti-inflammatory, anti platelet-aggregation and anti-obesity properties. Cinchonine inhibits cells proliferation and autophagy and induces apoptosis through activation of Caspase-3. Cinchonine activates endoplasmic reticulum stress-induced apoptosis in human liver cancer cells[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
HeLa EC50
697 μg/mL
Compound: I
Cytotoxicity against human HeLa cells by MTT assay
Cytotoxicity against human HeLa cells by MTT assay
[PMID: 22846917]
WI-38 IC50
> 20 μg/mL
Compound: 1c
Cytotoxicity against human WI38 cells by MTT assay
Cytotoxicity against human WI38 cells by MTT assay
[PMID: 23816880]
WI-38 IC50
> 68.03 μM
Compound: CN
Cytotoxicity against human WI38 cells by MTT assay
Cytotoxicity against human WI38 cells by MTT assay
[PMID: 26063305]
体外研究
(In Vitro)

Cinchonine 可以抑制 HepG2 和 SMCC7721 (180 μM, 24 h) 以及 HeLa 和 A549 (180 μM, 48-96 h) 细胞的增殖,诱导细胞凋亡[2][3]
Cinchonine (50-100 μM, 24 h) 在 A549 和 H1975 肺癌细胞中抑制肿瘤生长,IC[50] 值分别为 76.67 和 87.44 μM[4]
Cinchonine (0-20 μM, 24 h) 通过对成熟溶酶体水解酶的抑制作用阻断自噬通路[4]
Cinchonine (20 μM) 诱导破骨细胞分化和成骨作用[6]
Cinchonine 通过抑制 Ca2+ 通量 (IC50: 300 μM) 和Kinase C (IC50: 20 μM) 来抑制血小板聚集[7]
Cinchonine 可以抑制 T. evansi 增殖 (0-100 μM, 72 h),24小时内的 IC50为 16.96 μM[8]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2][3][4]

Cell Line: HeLa, A549, HepG2, SMCC7721, H1975
Concentration: 180 μM (HeLa, A549, HepG2, SMCC7721), 200 μM (A549 and H1975)
Incubation Time: 24 h-96 h (HeLa and A549), 48 h (HepG2 and SMCC7721), 24 h (A549 and H1975)
Result: Inhibited cell proliferation in cells HeLa, A549, HepG2 and SMCC7721.
Cinchonine inhibited phosphorylation of AKT and TAK1.
Cinchonine activated Casapase-3, promoted GRP78 and phosphorylation of PERK and sIF2-α.
Cinchonine reduced mature cathepsin levels and increased immature cathespin levels.
体内研究
(In Vivo)

Cinchonine (0.265-0.530 mg/kg,瘤内注射,LLC细胞,14 天) 可以诱导细胞凋亡,抑制 BALB/c 裸鼠体内的肿瘤生长,具有低毒性[2]
Cinchonine (4 mg/kg/day,19 天,腹腔注射,LLC 细胞) 对 BLAB/c 裸鼠的肺癌细胞具有低毒性的抗转移活性[4]
Cinchonine (50mg/kg,i.p.) 在 C57BL/6B 小鼠体内具有抗肥胖活性[5]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice [4]
Dosage: 4 mg/kg/day
Administration: Intraperitoneal injection, for 19 days
Result: Inhibited metastatic activity
Animal Model: BALB/c nude mice [2]
Dosage: 0.265 and 0.530 mg/kg
Administration: Intratumorally injection, for 14 days
Result: Inhibited tumor growth with more TUNEL positive cells (DNA fragmentation indicator).
Animal Model: High-fat-diet (HFD) induced obesity in C57BL/6B mice[5]
Dosage: 50 mg/kg
Administration: Intraperitoneal injection
Result: Reduced body weight gain (−38%), visceral fat-pad weights (−26%).
分子量

294.39

Formula

C19H22N2O

CAS 号
性状

固体

颜色

White to off-white

中文名称

辛可宁

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 4.76 mg/mL (16.17 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3969 mL 16.9843 mL 33.9685 mL
5 mM 0.6794 mL 3.3969 mL 6.7937 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.56%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3969 mL 16.9843 mL 33.9685 mL 84.9214 mL
5 mM 0.6794 mL 3.3969 mL 6.7937 mL 16.9843 mL
10 mM 0.3397 mL 1.6984 mL 3.3969 mL 8.4921 mL
15 mM 0.2265 mL 1.1323 mL 2.2646 mL 5.6614 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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