Docetaxel (Synonyms: 多西他赛; RP-56976)

目录号: HY-B0011 纯度: 99.91%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Docetaxel (RP-56976) 是一种微管解聚 (microtubule depolymerization) 抑制剂，其 IC₅₀ 值为 0.2 μM。Docetaxel 是紫杉醇的半合成类似物，能减弱 bcl-2 和 bcl-xL 基因表达的影响。Docetaxel 阻滞 G2/M 细胞周期，导致细胞凋亡 (apoptosis)。Docetaxel 具有抗肿瘤活性。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Docetaxel Chemical Structure

CAS No. : 114977-28-5

1. 客户无需承担相应的运输费用。

2. 同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

可免费申领三个不同产品的试用装。

3. 试用装只面向终端客户。

规格	价格	是否有货
10 mM * 1 mL in DMSO	￥737	In-stock
25 mg	￥670	In-stock
50 mg	￥990	In-stock
100 mg	￥1750	In-stock
200 mg	￥2800	In-stock
500 mg	￥4900	In-stock
1 g		询价
5 g		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

Other Forms of Docetaxel:

MCE 顾客使用本产品发表的 101 篇科研文献

: Docetaxel purchased from MCE. Usage Cited in: Arch Biochem Biophys. 2023 Feb 21;109551. [Abstract]; Docetaxel (30 nM) exhibits anti-tumor activity in parental (DU145 and PC-3) and docetaxel-resistant (DU145/DTX50 and PC-3/DTX30) cell lines by down-regulating KIF14 expression.

: Docetaxel purchased from MCE. Usage Cited in: Oncotarget. 2016 May 17;7(20):28891-902. [Abstract]; Western blot analyses for Brachyury and AR expression in prostate cell lines treated with Docetaxel (Doc) or with XRP6258 (Cab). Graphs represent the densimetric semi-quantification of western blot images.

Docetaxel 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Docetaxel (RP-56976) is a microtubule depolymerization inhibitor, with an IC₅₀ of 0.2 μM. Docetaxel attenuates the effects of bcl-2 and bcl-xL gene expression. Docetaxel arrests the cell cycle at G2/M and leads to cell apoptosis. Docetaxel has anti-cancer activity^[1]^[3].

IC₅₀ & Target

Microtubule^[1]

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A2780	IC₅₀	11.2 nM Compound: 1b	Cytotoxicity against human A2780 cells assessed as decrease in cell viability after 48 hrs by MTT assay Cytotoxicity against human A2780 cells assessed as decrease in cell viability after 48 hrs by MTT assay	[PMID: 29359935]
A549	IC₅₀	19.5 nM Compound: Docetaxel	Cytotoxicity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay Cytotoxicity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay	[PMID: 34058663]
A549	IC₅₀	3.41 μM Compound: DTX	Cytotoxicity against human A-549 cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control Cytotoxicity against human A-549 cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control	[PMID: 26791014]
A549	IC₅₀	734 nM Compound: Docetaxel	Cytotoxicity against human paclitaxl resistant A549 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human paclitaxl resistant A549 cells after 72 hrs by sulforhodamine B assay	[PMID: 30036834]
A549	IC₅₀	9.8 nM Compound: Docetaxel	Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay	[PMID: 30036834]
A549/TR	IC₅₀	836.2 nM Compound: Docetaxel	Cytotoxicity against human A549/Taxol cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay Cytotoxicity against human A549/Taxol cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay	[PMID: 34058663]
Calu-1	IC₅₀	0.4 nM Compound: Docetaxel	Antiproliferative activity against human Calu1 cells after 72 hrs by MTT assay Antiproliferative activity against human Calu1 cells after 72 hrs by MTT assay	[PMID: 30629433]
CAPAN-1	IC₅₀	0.0088 μM Compound: Docetaxel	Antiproliferative activity against human CAPAN-1 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis Antiproliferative activity against human CAPAN-1 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis	[PMID: 33248847]
CAPAN-1	IC₅₀	0.025 μM Compound: Docetaxel	Antiproliferative activity against human Capan1 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against human Capan1 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay	[PMID: 32832030]
CCD-18Co	IC₅₀	116.54 μM Compound: DTX	Cytotoxicity against human CCD-18Co cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human CCD-18Co cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32203786]
CWR22R	CC₅₀	1 nM Compound: Docetaxel	Cytotoxicity against human 22Rv1 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human 22Rv1 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 34717125]
CWR22R	CC₅₀	3.3 nM Compound: Docetaxel	Cytotoxicity against human 22Rv1 cells expressing PMSA measured using MTT assay Cytotoxicity against human 22Rv1 cells expressing PMSA measured using MTT assay	[PMID: 34797052]
CWR22R	GI₅₀	6 ng/mL Compound: Dxtl	Growth inhibition of human 22Rv1 cells incubated for 48 hrs by MTS assay Growth inhibition of human 22Rv1 cells incubated for 48 hrs by MTS assay	[PMID: 31767266]
DU-145	IC₅₀	0.0012 μM Compound: Docetaxel	Antiproliferative activity against androgen-insensitive human DU-145 cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay Antiproliferative activity against androgen-insensitive human DU-145 cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay	[PMID: 33753258]
DU-145	IC₅₀	0.0012 μM Compound: Docetaxel	Antiproliferative activity against docetaxel-sensitive human DU145 cells after 3 days by WST-1 assay Antiproliferative activity against docetaxel-sensitive human DU145 cells after 3 days by WST-1 assay	[PMID: 29784275]
DU-145	IC₅₀	0.01 μM Compound: Docetaxel	Antiproliferative activity in human DU145 cells by sulforhodamine B assay Antiproliferative activity in human DU145 cells by sulforhodamine B assay	[PMID: 29120172]
DU-145	IC₅₀	0.026 μM Compound: Docetaxel	Cytotoxicity against human DU145 cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against human DU145 cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 27077446]
DU-145	IC₅₀	0.1 nM Compound: Docetaxel	Cytotoxicity against human DU145 cells after 72 hrs by MTS assay Cytotoxicity against human DU145 cells after 72 hrs by MTS assay	[PMID: 29406710]
DU-145	CC₅₀	0.81 μM Compound: Docetaxel	Cytotoxic activity in human DU145 cells by sulforhodamine B assay Cytotoxic activity in human DU145 cells by sulforhodamine B assay	[PMID: 29120172]
DU-145	IC₅₀	4.95 nM Compound: Docetaxel	Cytotoxicity against human DU-145 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human DU-145 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31945665]
DU-145	IC₅₀	43.25 nM Compound: Docetaxel	Cytotoxicity against human DU-145 cells over expressing CYP1B1 assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human DU-145 cells over expressing CYP1B1 assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31945665]
DU-145	IC₅₀	5 μg/mL Compound: Docetaxel	Cytotoxicity against human DU-145 cells assessed as inhibition of cell growth by MTT assay Cytotoxicity against human DU-145 cells assessed as inhibition of cell growth by MTT assay	[PMID: 34000484]
DU-145	IC₅₀	8.58 μM Compound: Docetaxel	Antiproliferative activity against Docetaxel-resistant human DU-145 cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay Antiproliferative activity against Docetaxel-resistant human DU-145 cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay	[PMID: 33753258]
HCT-116	IC₅₀	0.0017 μM Compound: Docetaxel	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis	[PMID: 33248847]
HCT-116	IC₅₀	0.007 μM Compound: Docetaxel	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay	[PMID: 32832030]
HCT-116	IC₅₀	129.8 nM Compound: Docetaxel	Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 28972762]
HCT-116	IC₅₀	3.5 nM Compound: DTX	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32203786]
HCT-116	IC₅₀	4 nM Compound: Docetaxel	Anticancer activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Anticancer activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 33221063]
HEK293	IC₅₀	0.11 μM Compound: Docetaxel	Antiproliferative activity in HEK293 cells by sulforhodamine B assay Antiproliferative activity in HEK293 cells by sulforhodamine B assay	[PMID: 29120172]
HEK293	CC₅₀	1.6 μM Compound: Docetaxel	Cytotoxic activity in HEK293 cells by sulforhodamine B assay Cytotoxic activity in HEK293 cells by sulforhodamine B assay	[PMID: 29120172]
HEK293	IC₅₀	19.7 μM Compound: DTX	Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 32203786]
HeLa	IC₅₀	0.061 μM Compound: Docetaxel	Cytotoxicity against human HeLa cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 27077446]
HeLa	IC₅₀	0.07 μM Compound: Docetaxel	Antiproliferative activity in human HeLa cells by sulforhodamine B assay Antiproliferative activity in human HeLa cells by sulforhodamine B assay	[PMID: 29120172]
HeLa	CC₅₀	0.09 μM Compound: Docetaxel	Cytotoxic activity in human HeLa cells by sulforhodamine B assay Cytotoxic activity in human HeLa cells by sulforhodamine B assay	[PMID: 29120172]
HeLa	IC₅₀	0.3 nM Compound: Docetaxel	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 30629433]
HeLa	IC₅₀	0.68 μM Compound: Docetaxel	Cytotoxicity against human HeLa cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay Cytotoxicity against human HeLa cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay	[PMID: 36126331]
HeLa	IC₅₀	13 μM Compound: Docetaxel	Growth inhibition of human HeLa cells over expressing beta3-tubulin measured after 96 hrs by sulforhodamine B assay Growth inhibition of human HeLa cells over expressing beta3-tubulin measured after 96 hrs by sulforhodamine B assay	[PMID: 27894589]
HeLa	IC₅₀	3.23 μM Compound: DTX	Cytotoxicity against human HeLa cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control Cytotoxicity against human HeLa cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control	[PMID: 26791014]
HeLa	IC₅₀	3.5 nM Compound: 1b	Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 48 hrs by MTT assay	[PMID: 29359935]
HeLa	IC₅₀	4 μM Compound: Docetaxel	Growth inhibition of human HeLa cells measured after 96 hrs by sulforhodamine B assay Growth inhibition of human HeLa cells measured after 96 hrs by sulforhodamine B assay	[PMID: 27894589]
HEp-2	CC₅₀	0.06 μM Compound: Docetaxel	Cytotoxic activity in human Hep2 cells by sulforhodamine B assay Cytotoxic activity in human Hep2 cells by sulforhodamine B assay	[PMID: 29120172]
HEp-2	IC₅₀	0.06 μM Compound: Docetaxel	Antiproliferative activity in human Hep2 cells by sulforhodamine B assay Antiproliferative activity in human Hep2 cells by sulforhodamine B assay	[PMID: 29120172]
HepG2	IC₅₀	2.86 μM Compound: DTX	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control Cytotoxicity against human HepG2 cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control	[PMID: 26791014]
HepG2	IC₅₀	8.3 μM Compound: Docetaxel	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 27617953]
HEY	IC₅₀	0.86 μM Compound: Taxotere	Cytotoxicity against human HEY cells after 72 to 120 hrs by sulforhodamine B assay Cytotoxicity against human HEY cells after 72 to 120 hrs by sulforhodamine B assay	[PMID: 29517223]
HL-60	IC₅₀	0.0072 μM Compound: Docetaxel	Antiproliferative activity against human HL-60 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis Antiproliferative activity against human HL-60 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis	[PMID: 33248847]
HL-60	IC₅₀	0.05 μM Compound: Docetaxel	Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay	[PMID: 32832030]
K562	IC₅₀	0.009 μM Compound: Docetaxel	Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay	[PMID: 32832030]
K562	IC₅₀	0.0152 μM Compound: Docetaxel	Antiproliferative activity against human K562 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis Antiproliferative activity against human K562 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis	[PMID: 33248847]
K562	IC₅₀	0.039 μM Compound: Docetaxel	Cytotoxicity against human K562 cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against human K562 cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 27077446]
KB	IC₅₀	0.05 μM Compound: Docetaxel	Antiproliferative activity in human KB cells by sulforhodamine B assay Antiproliferative activity in human KB cells by sulforhodamine B assay	[PMID: 29120172]
KB	GI₅₀	0.13 nM Compound: Docetaxel	Growth inhibition of human KB cells after 72 hrs by MTS assay Growth inhibition of human KB cells after 72 hrs by MTS assay	[PMID: 26778612]
KB	CC₅₀	0.18 μM Compound: Docetaxel	Cytotoxic activity in human KB cells by sulforhodamine B assay Cytotoxic activity in human KB cells by sulforhodamine B assay	[PMID: 29120172]
KB	IC₅₀	17.5 nM Compound: Docetaxel	Cytotoxicity against human KB cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay Cytotoxicity against human KB cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay	[PMID: 34058663]
KB	IC₅₀	38 nM Compound: Docetaxel	Cytotoxicity against human KB cells by MTT assay Cytotoxicity against human KB cells by MTT assay	[PMID: 30407813]
KB	IC₅₀	7.3 nM Compound: Docetaxel	Cytotoxicity against human KB cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human KB cells after 72 hrs by sulforhodamine B assay	[PMID: 30036834]
L132	IC₅₀	1.68 μM Compound: DTX	Cytotoxicity against human L-132 cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control Cytotoxicity against human L-132 cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control	[PMID: 26791014]
LN-229	IC₅₀	0.002 μM Compound: Docetaxel	Antiproliferative activity against human LN229 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against human LN229 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay	[PMID: 32832030]
LNCaP	IC₅₀	0.0002 μM Compound: Docetaxel	Antiproliferative activity against androgen-insensitive human LNCaP cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay Antiproliferative activity against androgen-insensitive human LNCaP cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay	[PMID: 33753258]
LNCaP	IC₅₀	0.0002 μM Compound: Docetaxel	Antiproliferative activity against docetaxel-sensitive human LNCAP cells after 3 days by WST-1 assay Antiproliferative activity against docetaxel-sensitive human LNCAP cells after 3 days by WST-1 assay	[PMID: 29784275]
MCF7	CC₅₀	0.01 μM Compound: Docetaxel	Cytotoxic activity in human MCF7 cells by sulforhodamine B assay Cytotoxic activity in human MCF7 cells by sulforhodamine B assay	[PMID: 29120172]
MCF7	IC₅₀	0.01 μM Compound: Docetaxel	Antiproliferative activity in human MCF7 cells by sulforhodamine B assay Antiproliferative activity in human MCF7 cells by sulforhodamine B assay	[PMID: 29120172]
MCF7	IC₅₀	0.022 μM Compound: Docetaxel	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 27077446]
MCF7	IC₅₀	0.5 nM Compound: Docetaxel	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 30629433]
MCF7	IC₅₀	0.8 μM Compound: Docetaxel	Cytotoxicity against human MCF7 cells assessed as inhibition of cell survival rate after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell survival rate after 48 hrs by MTT assay	[PMID: 27240278]
MCF7	IC₅₀	1.7 nM Compound: Docetaxel	Cytotoxicity against human MCF7 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human MCF7 cells after 72 hrs by sulforhodamine B assay	[PMID: 30036834]
MCF7	IC₅₀	10.3 nM Compound: Docetaxel	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 30407813]
MCF7	IC₅₀	3.6 μM Compound: DTX	Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability at 10 uM measured after 24 hrs by MTT assay relative to control	[PMID: 26791014]
MDA-MB-231	IC₅₀	0.01 μM Compound: Docetaxel	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs	[PMID: 33139111]
MDA-MB-231	IC₅₀	0.032 μM Compound: Docetaxel	Cytotoxicity against human MDA-MB-231 cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 27077446]
MDA-MB-231	IC₅₀	0.07 μM Compound: Docetaxel	Antiproliferative activity in human MDA-MB-231 cells by sulforhodamine B assay Antiproliferative activity in human MDA-MB-231 cells by sulforhodamine B assay	[PMID: 29120172]
MDA-MB-231	CC₅₀	0.19 μM Compound: Docetaxel	Cytotoxic activity in human MDA-MB-231 cells by sulforhodamine B assay Cytotoxic activity in human MDA-MB-231 cells by sulforhodamine B assay	[PMID: 29120172]
MM1.S	IC₅₀	0.0118 μM Compound: Docetaxel	Antiproliferative activity against human MM1.S cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis Antiproliferative activity against human MM1.S cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis	[PMID: 33248847]
MV4-11	GI₅₀	0.0005 μM Compound: Taxotere	Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by CellTiter-Glo luminescence assay Antiproliferative activity against human MV4-11 cells incubated for 72 hrs by CellTiter-Glo luminescence assay	[PMID: 30802730]
NCI/ADR-RES	IC₅₀	1530.5 nM Compound: Docetaxel	Cytotoxicity against human MCF7/ADR cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human MCF7/ADR cells after 72 hrs by sulforhodamine B assay	[PMID: 30036834]
NCI/ADR-RES	IC₅₀	21.2 μM Compound: Docetaxel	Cytotoxicity against human MCF7/ADR cells assessed as inhibition of cell survival rate after 48 hrs by MTT assay Cytotoxicity against human MCF7/ADR cells assessed as inhibition of cell survival rate after 48 hrs by MTT assay	[PMID: 27240278]
NCI-H460	IC₅₀	0.0024 μM Compound: Docetaxel	Antiproliferative activity against human NCI-H460 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis Antiproliferative activity against human NCI-H460 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis	[PMID: 33248847]
NCI-H460	IC₅₀	0.004 μM Compound: Docetaxel	Antiproliferative activity against human NCI-H460 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against human NCI-H460 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay	[PMID: 32832030]
PC-3	IC₅₀	0.0006 μM Compound: Docetaxel	Cytotoxicity against human PC-3 cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay Cytotoxicity against human PC-3 cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay	[PMID: 36126331]
PC-3	IC₅₀	0.0019 μM Compound: Docetaxel	Antiproliferative activity against androgen-insensitive human PC-3 cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay Antiproliferative activity against androgen-insensitive human PC-3 cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay	[PMID: 33753258]
PC-3	IC₅₀	0.0019 μM Compound: Docetaxel	Antiproliferative activity against docetaxel-sensitive human PC3 cells after 3 days by WST-1 assay Antiproliferative activity against docetaxel-sensitive human PC3 cells after 3 days by WST-1 assay	[PMID: 29784275]
PC-3	IC₅₀	0.013 μM Compound: Docetaxel	Cytotoxicity against human PC3 cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 27077446]
PC-3	IC₅₀	0.5 nM Compound: Docetaxel	Cytotoxicity against human PC3 cells after 72 hrs by MTS assay Cytotoxicity against human PC3 cells after 72 hrs by MTS assay	[PMID: 29406710]
PC-3	IC₅₀	0.6 nM Compound: Docetaxel	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 30629433]
PC-3	CC₅₀	1 nM Compound: Docetaxel	Cytotoxicity against human PC-3 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human PC-3 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 34717125]
PC-3	IC₅₀	2.34 μM Compound: Docetaxel	Antiproliferative activity against Docetaxel-resistant human PC-3 cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay Antiproliferative activity against Docetaxel-resistant human PC-3 cells assessed as reduction in cell viability incubated for 3 days by WST-1 assay	[PMID: 33753258]
PC-3	CC₅₀	6.7 nM Compound: Docetaxel	Cytotoxicity against human PC-3 cells not expressing PMSA measured using MTT assay Cytotoxicity against human PC-3 cells not expressing PMSA measured using MTT assay	[PMID: 34797052]
PC-3	IC₅₀	8 nM Compound: Docetaxel	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 72 hrs by trypan blue exclusion assay Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 72 hrs by trypan blue exclusion assay	[PMID: 28462831]
PC-3	GI₅₀	9 ng/mL Compound: Dxtl	Growth inhibition of human PC3 cells incubated for 48 hrs by MTS assay Growth inhibition of human PC3 cells incubated for 48 hrs by MTS assay	[PMID: 31767266]
RWPE-1	IC₅₀	1.73 μM Compound: Docetaxel	Cytotoxicity against human RWPE1 cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against human RWPE1 cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 27077446]
SH-SY5Y	IC₅₀	0.0018 μM Compound: Docetaxel	Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell growth and measured upto 72 hrs by clonogenic assay Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell growth and measured upto 72 hrs by clonogenic assay	[PMID: 36126331]
SiHa	IC₅₀	0.09 μM Compound: Docetaxel	Antiproliferative activity in human SiHa cells by sulforhodamine B assay Antiproliferative activity in human SiHa cells by sulforhodamine B assay	[PMID: 29120172]
SiHa	CC₅₀	0.22 μM Compound: Docetaxel	Cytotoxic activity in human SiHa cells by sulforhodamine B assay Cytotoxic activity in human SiHa cells by sulforhodamine B assay	[PMID: 29120172]
SK-MEL19	GI₅₀	0.002 μM Compound: Taxotere	Antiproliferative activity against human SK-MEL-19 cells incubated for 72 hrs by CellTiter-Glo luminescence assay Antiproliferative activity against human SK-MEL-19 cells incubated for 72 hrs by CellTiter-Glo luminescence assay	[PMID: 30802730]
SK-OV-3	IC₅₀	0.75 nM Compound: Taxotere	Cytotoxicity against human SKOV3 cells after 72 to 120 hrs by sulforhodamine B assay Cytotoxicity against human SKOV3 cells after 72 to 120 hrs by sulforhodamine B assay	[PMID: 29517223]
SW-620	IC₅₀	0.02 μM Compound: DTX	Cytotoxicity against human SW-620 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human SW-620 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32203786]
SW-620	IC₅₀	4.7 nM Compound: Docetaxel	Anticancer activity against human SW-620 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Anticancer activity against human SW-620 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 33221063]
T84	IC₅₀	2.7 nM Compound: Docetaxel	Anticancer activity against human T84 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Anticancer activity against human T84 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 33221063]
TERT-RPE1	IC₅₀	0.0553 μM Compound: Docetaxel	Cytotoxicity against human TERT-RPE1 cells assessed as inhibition of cell proliferation measured after 72 hrs by real time imaging analysis Cytotoxicity against human TERT-RPE1 cells assessed as inhibition of cell proliferation measured after 72 hrs by real time imaging analysis	[PMID: 33248847]
U-251	IC₅₀	0.7 nM Compound: Docetaxel	Antiproliferative activity against human U251MG cells after 72 hrs by MTT assay Antiproliferative activity against human U251MG cells after 72 hrs by MTT assay	[PMID: 30629433]
Vero	IC₅₀	0.13 μM Compound: Docetaxel	Antiproliferative activity in African green monkey Vero cells by sulforhodamine B assay Antiproliferative activity in African green monkey Vero cells by sulforhodamine B assay	[PMID: 29120172]
Vero	CC₅₀	1.3 μM Compound: Docetaxel	Cytotoxic activity in African green monkey Vero cells by sulforhodamine B assay Cytotoxic activity in African green monkey Vero cells by sulforhodamine B assay	[PMID: 29120172]
Z-138	IC₅₀	0.004 μM Compound: Docetaxel	Antiproliferative activity against human Z138 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against human Z138 cells assessed as reduction in cell viability incubated for 72 hrs by IncuCyte live-cell imaging assay	[PMID: 32832030]
Z-138	IC₅₀	0.0142 μM Compound: Docetaxel	Antiproliferative activity against human Z138 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis Antiproliferative activity against human Z138 cells assessed as inhibition of cell proliferation measured after 72 hrs by by real time imaging analysis	[PMID: 33248847]

体外研究
(In Vitro)

Docetaxel (RP-56976) 和葡磷酰胺 (GLU) 的单一和联合处理以剂量依赖的方式影响细胞活力。GLU在PC-3和LNCaP细胞中的IC₅₀分别为70±4 μM和86.8±8 μM。同时，在 PC-3 和 LNCaP 细胞中，单用 Docetaxel 的 IC₅₀ 分别为 3.08±0.4 nM 和 1.46±0.2 nM
GLU与 Docetaxel 联合处理可协同细胞毒性，PC-3 和 LNCaP 细胞的 IC₅₀ 值分别降低至2.7±0.1 nM和0.75±0.3 nM^[1]。
Docetaxel 对 NCI-H460 的 IC₅₀ 在24小时为116 nM，在72小时为30 nM。根据 DTP 数据搜索中报告的数据，NCI-60 细胞板对 Docetaxel 的平均 IC₅₀ 为 14-34 nM^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

在雌性小鼠中，光照后 14 小时 (HALO) 组中 Docetaxel (RP-56976) 诱导的肠细胞凋亡明显大于 2-HALO 组。Bax 表达在 2-HALO 组中被 Docetaxel 显著升高，但在 14-HALO 组中没有。另一方面，Docetaxel 在 14-HALO 组中显著升高了裂解的 Caspase-3 表达，但在 2-HALO 组中没有。Wee1 和磷酸化 CKD1 的表达在 Docetaxel 给药后在 14 HALO 时显著升高，但在 2 HALO 时没有。此外，Docetaxel 显著降低 14-HALO 组中的存活蛋白表达，但不降低 2-HALO 组中的存活蛋白表达。Docetaxel 处理14-HALO组 survivin 表达水平明显低于药物处理2-HALO组^[3]。
胡椒碱 (PIP) 通过静脉给药以 3.5 mg/kg 推注并以 35 mg/kg 和 3.5 mg/kg 口服给药，而 Docetaxel (DOX) 以 7 mg/kg 静脉内给药至 Sprague-Daley 大鼠。在 Sprague-Dawley 大鼠中通过口服给药 35 mg/kg 的 PIP 和通过静脉推注给药 7 mg/kg 的 Docetaxel 共同给药。PIP 和 Docetaxel 的联合使用导致它们体内暴露的协同增加^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

807.88

Formula

C₄₃H₅₃NO₁₄

CAS 号

114977-28-5

性状

固体

颜色

White to off-white

中文名称

多西他赛；多西紫杉醇

结构分类

Others

初始来源

内源性代谢物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 100 mg/mL (123.78 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

Ethanol 中的溶解度 : 50 mg/mL (61.89 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.2378 mL	6.1890 mL	12.3781 mL
5 mM	0.2476 mL	1.2378 mL	2.4756 mL
10 mM	0.1238 mL	0.6189 mL	1.2378 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (6.19 mM); 澄清溶液

此方案可获得 ≥ 5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 EtOH 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% EtOH 90% Corn Oil
Solubility: ≥ 5 mg/mL (6.19 mM); 澄清溶液

此方案可获得 ≥ 5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 EtOH 储备液加到 900 μL 玉米油中，混合均匀。
方案三

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案四

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案五

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案六

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案七

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案八

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案九

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
方案十一

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
方案十一一

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
方案十一二

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.57 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.91%

选择批次：

Data Sheet (665 KB) SDS (558 KB)

COA (297 KB) LCMS (96 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Attia RT, et al. The chemomodulatory effects of glufosfamide on docetaxel cytotoxicity in prostate cancer cells. PeerJ. 2016 Jun 29;4:e2168. [Content Brief]

[2]. Che CL, et al. DNA microarray reveals different pathways responding to NSC 125973 and docetaxel in non-small cell lung cancer cell line. Int J Clin Exp Pathol. 2013 Jul 15;6(8):1538-48. [Content Brief]

[3]. Obi-Ioka Y, et al. Involvement of Wee1 in the circadian rhythm dependent intestinal damage induced by docetaxel. J Pharmacol Exp Ther. 2013 Oct;347(1):242-8. [Content Brief]

[4]. Li C, et al. Non-linear pharmacokinetics of piperine and its herb-drug interactions with docetaxel in Sprague-Dawley rats. J Pharm Biomed Anal. 2016 Sep 5;128:286-93. [Content Brief]

Cell Assay ^[1]	Single-drug concentration-response curves are assessed. Seeding is done at a density of 2,000 cells/well for PC-3 and LNCaP, in 96-well plates. Cells are treated with each single drug and their combination for 72 h at different drug concentrations. Docetaxel is used at concentrations of 0.1-1,000 nM. GLU is used at concentrations of 0.1-300 µm. Cytotoxicity is assessed at the end of drug exposure using SRB assay. Following 72 h exposure the cells are fixed with 10% trichloroacetic acid (150 µL) for 1 h at 4°C. Then, cells are stained for 10 min at room temperature with 0.4% SRB dissolved in 1% acetic acid. The plates are then air dried for 24 h and the dye is made soluble with 150 µL Tris (10 mM, PH 7.4) for 5 min on a shaker at 1,600 rpm. Absorbance is then measured at 545 nM using microplate reader. Results are expressed as the relative percentage of absorbance compared to control^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]^[4]	Mice^[3] Five-week-old male Balb/c mice are used. Docetaxel (0, 10, 20, 30, 40, 60, and 80 mg/kg per week) is given once a week for 3 weeks for mice. Because more than 30 mg/kg per week of Docetaxel causes body weight loss in mice, 20 mg/kg per week of Docetaxel is judged to be the maximum nontoxic dose. Docetaxel (20 mg/kg per week) is given to mice once a week for 3 weeks at one of the following different points (2, 10, 14, or 22 HALO). Seventy-two hours after the final dosing of the agent, the intestinal mucosa of the small intestine (proximal 8 cm) is removed, fixed in 20 N Mildform solution (containing 8% formaldehyde in a buffered solution), and embedded in paraffin blocks, and sections of 5 μm are put on glass slides. Apoptosis is detected using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) method, using the Apop Tag Peroxidase In Situ Apoptosis Detection Kit. Rats^[4] Male Sprague-Dawley rats with body weight between 230-250 g and age between 6-7 weeks are used. About 25 SD rats are divided into five groups receiving Docetaxel (7 mg/kg, i.v.), PIP (35 mg/kg, p.o.) and their combined administration (DOX+PIP) as well as PIP (3.5 mg/kg, p.o.) and PIP (3.5 mg/kg, i.v.). A day before the drug administrations, the rats are anesthetized. Right jugular vein is cannulated with a polyethylene tubing (0.5 mm ID, 1 mm) for blood collection. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Attia RT, et al. The chemomodulatory effects of glufosfamide on docetaxel cytotoxicity in prostate cancer cells. PeerJ. 2016 Jun 29;4:e2168. [Content Brief] [2]. Che CL, et al. DNA microarray reveals different pathways responding to NSC 125973 and docetaxel in non-small cell lung cancer cell line. Int J Clin Exp Pathol. 2013 Jul 15;6(8):1538-48. [Content Brief] [3]. Obi-Ioka Y, et al. Involvement of Wee1 in the circadian rhythm dependent intestinal damage induced by docetaxel. J Pharmacol Exp Ther. 2013 Oct;347(1):242-8. [Content Brief] [4]. Li C, et al. Non-linear pharmacokinetics of piperine and its herb-drug interactions with docetaxel in Sprague-Dawley rats. J Pharm Biomed Anal. 2016 Sep 5;128:286-93. [Content Brief]

Docetaxel 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	1.2378 mL	6.1890 mL	12.3781 mL	30.9452 mL
	5 mM	0.2476 mL	1.2378 mL	2.4756 mL	6.1890 mL
	10 mM	0.1238 mL	0.6189 mL	1.2378 mL	3.0945 mL
	15 mM	0.0825 mL	0.4126 mL	0.8252 mL	2.0630 mL
	20 mM	0.0619 mL	0.3095 mL	0.6189 mL	1.5473 mL
	25 mM	0.0495 mL	0.2476 mL	0.4951 mL	1.2378 mL
	30 mM	0.0413 mL	0.2063 mL	0.4126 mL	1.0315 mL
	40 mM	0.0309 mL	0.1547 mL	0.3095 mL	0.7736 mL
	50 mM	0.0248 mL	0.1238 mL	0.2476 mL	0.6189 mL
	60 mM	0.0206 mL	0.1032 mL	0.2063 mL	0.5158 mL
DMSO	80 mM	0.0155 mL	0.0774 mL	0.1547 mL	0.3868 mL
DMSO	100 mM	0.0124 mL	0.0619 mL	0.1238 mL	0.3095 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Docetaxel114977-28-5RP-56976多西他赛多西紫杉醇RP56976RP 56976Microtubule/TubulinApoptosisEndogenous MetaboliteInhibitorinhibitorinhibit

产品名称：			* 需求量：
* 客户姓名：			* Email：
* 电话：			* 公司或机构名称：
留言给我们：

产品名称：			* Lot #：
* 客户姓名：			* Email：
电话：			* 部门：
* 公司或机构名称：
留言给我们：

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。	站点地图隐私声明
沪ICP备15051369号-4 上海工商沪公网安备31011502019417 沪(浦)应急管危经许[2021]201709(QFYS) 营业执照（三证合一）
Copyright © 2013-2024 MedChemExpress. All Rights Reserved.

Docetaxel (Synonyms: 多西他赛; RP-56976)

Customer Review

Other Forms of Docetaxel:

MCE 顾客使用本产品发表的 101 篇科研文献

Docetaxel 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

摩尔计算器

稀释计算器

Docetaxel 相关分类

完整储备液配制表

Keywords:

您最近查看的产品:

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

姓名
邮箱 *

Sorry, but the email address you supplied was invalid.

Docetaxel (Synonyms: 多西他赛; RP-56976)

Customer Review

Other Forms of Docetaxel:

Docetaxel 相关抗体

MCE 顾客使用本产品发表的 101 篇科研文献

Docetaxel 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

Docetaxel 相关抗体:

摩尔计算器

稀释计算器

Docetaxel 相关分类

完整储备液配制表

Keywords:

您最近查看的产品:

Request for HNMR Report

Request for HNMR Report

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z