1. Stem Cell/Wnt TGF-beta/Smad PI3K/Akt/mTOR Apoptosis
  2. TGF-beta/Smad PI3K Apoptosis
  3. Disitertide diammonium

Disitertide diammonium  (Synonyms: P144 diammonium)

目录号: HY-P0118B 纯度: 99.53%
COA 产品使用指南

Disitertide (P144) diammonium 是转化生长因子 TGFβ1 的多肽抑制剂,特异性的阻断其与受体间的相互作用。Disitertide diammonium 也是PI3K 的抑制剂和凋亡 (apoptosis) 诱导剂。

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Custom Peptide Synthesis

Disitertide diammonium Chemical Structure

Disitertide diammonium Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
1 mg ¥950
In-stock
5 mg ¥2900
In-stock
10 mg ¥5050
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Disitertide diammonium:

    Disitertide diammonium purchased from MCE. Usage Cited in: Cell Death Differ. 2021 Jan;28(1):219-232.  [Abstract]

    LINC00941-overexpressing cells treated with Disitertide (10 μM) shows increased mRNA and protein levels of ZO-1 and Ecadherin compared with controls. LINC00941-silenced cells show decreased mRNA and protein levels of ZO-1 and E-cadherin but increased mRNA and protein levels of Vimentin, Fibronectin, and Twist1.
    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Disitertide (P144) diammonium is a peptidic transforming growth factor-beta 1 (TGF-β1) inhibitor specifically designed to block the interaction with its receptor. Disitertide diammonium is also a PI3K inhibitor and an apoptosis inducer[1][2][3][4][5].

    体外研究
    (In Vitro)

    Disitertide (P144, 100 μg/mL) diammonium suppresses the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells[2].
    Disitertide (TGF-β1 inhibitor) diammonium abrogates the MACC1- AS1 expression in GC cells, suggesting that targeting TGFβ signaling pathway may be a potential strategy to inhibit MSC-induced stemness and chemoresistance[3].
    Disitertide (10 μg/mL to 200 μg/mL) diammonium affects proliferation, induces apoptosis as well as anoikis in A172 and U-87 MG GBM cell lines[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[2]

    Cell Line: Mouse embryo osteoblast precursor MC3T3-E1 cells.
    Concentration: 100 μg/mL
    Incubation Time: 4 h
    Result: Significantly suppressed the protein expression levels of PI3K and p-Akt, and induce the protein expression of Bax in MC3T3-E1 cells compared with the miR-590 group.
    体内研究
    (In Vivo)

    Disitertide (P144, Topical application, 300 μg/mL) diammonium may promote scar maturation and improvement of hypertrophic scar morphology features in an “in vivo” model in nude mice after two weeks of treatment[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Human hypertrophic scars were implanted in 60 nude mice[4].
    Dosage: 300 μg/mL was added the Lipogel.
    Administration: Topical application daily administered.
    Result: Successful shedding was achieved in 83.3% of the xenografts.
    Clinical Trial
    分子量

    1614.88

    Formula

    C68H115N19O22S2

    性状

    固体

    颜色

    White to off-white

    Sequence Shortening

    TSLDASIIWAMMQN

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    溶解性数据
    细胞实验: 

    DMSO 中的溶解度 : 10 mg/mL (6.19 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    H2O 中的溶解度 : 9.09 mg/mL (5.63 mM; ultrasonic and adjust pH to 9 with NH3·H2O)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 0.6192 mL 3.0962 mL 6.1924 mL
    5 mM 0.1238 mL 0.6192 mL 1.2385 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验:

    以下溶解方案,请直接配制工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: 50% PEG300    50% Saline

      Solubility: 5 mg/mL (3.10 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.53%

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 0.6192 mL 3.0962 mL 6.1924 mL 15.4810 mL
    5 mM 0.1238 mL 0.6192 mL 1.2385 mL 3.0962 mL

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Inquiry Information

    产品名称:
    Disitertide diammonium
    目录号:
    HY-P0118B
    需求量: