Idebenone (Synonyms: 艾地苯醌)

目录号: HY-N0303 纯度: 99.36%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Idebenone，一种线粒体保护剂，具有神经保护功效，可用于研究阿尔茨海默病、亨廷顿舞蹈病，可透过血脑屏障。Idebenone (氧化形式) 对星形胶质细胞匀浆中花生四烯酸的酶代谢具有抑制作用 (IC₅₀=16.65 μM)。 Idebenone 是一种辅酶 Q10 类似物和抗氧化剂，在人多巴胺能神经母细胞瘤 SHSY-5Y 细胞中诱导细胞凋亡 (apoptotic)。

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Idebenone Chemical Structure

CAS No. : 58186-27-9

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥500	In-stock
1 mg	￥125	In-stock
5 mg	￥250	In-stock
10 mg	￥400	In-stock
50 mg	￥1000	In-stock
100 mg	￥1400	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

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Customer Review

Other Forms of Idebenone:

Idebenone (Standard) In-stock

MCE 顾客使用本产品发表的 6 篇科研文献

: Idebenone purchased from MCE. Usage Cited in: Oncotarget. 2018 Jan 30;9(15):12137-12153. [Abstract]; Retinal protection of Rasagiline combined with Idebenone against retinal ischemia-reperfusion (RIR) injury. Western blot analysis for the expression level of brain-derived neurotrophic factor (BDNF) in the whole IR-injured retinas.

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生物活性
纯度 & 产品资料
参考文献

生物活性

Idebenone, a well-appreciated mitochondrial protectant, exhibits protective efficacy against neurotoxicity and can be used for the research of Alzheimer's disease, Huntington's disease. Idebenone (oxidised form) has a dose-dependent inhibitory effect on the enzymatic metabolism of arachidonic acid in astroglial homogenates (IC₅₀=16.65 μM)^[1]. Idebenone, a coenzyme Q10 analog and an antioxidant, induces apoptotic cell death in the human dopaminergic neuroblastoma SHSY-5Y cells^[2]. Idebenone quickly crosses the blood-brain barrier.

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
Fibroblast	EC₅₀	592 nM Compound: Idebenone	Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay Cytoprotective activity against BSO-induced human FRDA primary patient fibroblasts compound pretreated 12 hrs prior to BSO-induction measured after 48 hrs by calcein-AM-based cell viability assay	[PMID: 22137789]
Fibroblast	GI₅₀	98 μM Compound: Idebenone	Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay Growth inhibition of human FRDA primary patient fibroblasts after 48 hrs by calcein-AM-based cell viability assay	[PMID: 22137789]
HepG2	EC₅₀	0.94 μM Compound: Idebenone	Suppression of rotenone-induced ATP depletion in human HepG2 cells incubated for 2 hrs by Celltiter-Glo assay Suppression of rotenone-induced ATP depletion in human HepG2 cells incubated for 2 hrs by Celltiter-Glo assay	[PMID: 33180495]
HepG2	IC₅₀	64.5 μM Compound: Idebenone	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-blue assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-blue assay	[PMID: 33180495]
HL-60	IC₅₀	> 200 μM Compound: 3	Antiproliferative activity against human mitochondrial gene-knockout HL60 cells after 48 hrs by MTT assay Antiproliferative activity against human mitochondrial gene-knockout HL60 cells after 48 hrs by MTT assay	[PMID: 23432053]
HL-60	IC₅₀	36 μM Compound: 3	Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay Antiproliferative activity against human HL60 cells after 48 hrs by MTT assay	[PMID: 23432053]
HL-60	IC₅₀	70 μM Compound: 3	Inhibition of tPMET complex in human mitochondrial gene-knockout HL60 cells after 30 mins by WST-1/mPMS reduction assay Inhibition of tPMET complex in human mitochondrial gene-knockout HL60 cells after 30 mins by WST-1/mPMS reduction assay	[PMID: 23432053]
HL-60	IC₅₀	77 μM Compound: 3	Inhibition of tPMET complex in human HL60 cells after 30 mins by WST-1/mPMS reduction assay Inhibition of tPMET complex in human HL60 cells after 30 mins by WST-1/mPMS reduction assay	[PMID: 23432053]
SH-SY5Y	IC₅₀	15.9 μM Compound: Idebenone	Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-blue assay Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 24 hrs by Celltiter-blue assay	[PMID: 33180495]

体外研究
(In Vitro)

Idebenone, a compound with protective efficacy against neurotoxicity both in in vitro and in in vivo models, exists in two different oxidative states: the ubiquinol-derivative (reduced idebenone) and the ubiquinone-derivative (oxidised idebenone)^[1].
Idebenone (oxidised form) preferentially inhibits cyclooxygenase vs. lipoxygenase metabolism (IC₅₀ ratio lipoxygenase/cyclooxygenase: 3.22)^[1].
Idebenone (oxidised form) behaves similarly as indomethacin and piroxicam—two typical anti-inflammatory agents^[1].
Idebenone (oxidised form) inhibits total arachidonic acid metabolism, cyclooxygenase metabolism, lipoxygenase metabolism, lipoxygenase/cyclooxygenase with IC₅₀s of 16.65±3.48, 14.44±2.99, 46.51±7.20, and 3.22 μM^[1].
Idebenone (1-10 μM; for 24-72 h) has no effect on the cell viability of SHSY-5Y cells^[2].
Idebenone (25 μM or higher concentrations; for 24-72 h) shows significant reduction in cell viability of SHSY-5Y cells^[2].
Idebenone (30 μM) induces up-regulation of BAX expression and the caspase-3 activity^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	The human dopaminergic neuroblastma cell line, SHSY-5Y cells
Concentration:	1, 3, 10, 15, 25, 30, and 90 μM
Incubation Time:	24, 48, and 72 hours
Result:	Had no apparent detrimental effects on cell viability as indicated by the absence of trypan blue-positive staining in the cells at concentrations of 1, 3, 10 μM. Exhibited some degree of trypan blue-positive staining at 15 μM. Showed extensive trypan blue-positive staining at 25 μM and 30 μM.

RT-PCR^[2]

Cell Line:	SHSY-5Y cells
Concentration:	10 μM, 30 μM
Incubation Time:	72 hours
Result:	The total RNA of BAX from SHSY-5Y cells exposed to 10 μM was not different from that of the untreated control cells. The BAX expression in SHSY-5Y cells exposed to 30 μM was significantly up-regulated when compared with the untreated control cells and cells exposed to 10 μM.

体内研究
(In Vivo)

Idebenone (oxidised form) is a compound with protective efficacy against cerebrovascular disorders in vivo, including cerebral ischemia and hypertension-induced vascular lesions^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

338.44

Formula

C₁₉H₃₀O₅

CAS 号

58186-27-9

性状

固体

颜色

Yellow to orange

中文名称

艾地苯醌

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 100 mg/mL (295.47 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9547 mL	14.7737 mL	29.5473 mL
5 mM	0.5909 mL	2.9547 mL	5.9095 mL
10 mM	0.2955 mL	1.4774 mL	2.9547 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.39 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.39 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.39 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.36%

选择批次：

Data Sheet (635 KB) SDS (393 KB)

COA (279 KB) LCMS (90 KB)

产品使用指南 (1538 KB)

参考文献

[1]. G Civenni, et al. Inhibitory effect of the neuroprotective agent idebenone on arachidonic acid metabolism in astrocytes.Eur J Pharmacol. 1999 Apr 9;370(2):161-7. [Content Brief]

[2]. Kwok-Keung Tai, et al. Idebenone induces apoptotic cell death in the human dopaminergic neuroblastoma SHSY-5Y cells. Neurotox Res. 2011 Nov;20(4):321-8. [Content Brief]

Idebenone 相关分类

Neurological Disease

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9547 mL	14.7737 mL	29.5473 mL	73.8683 mL
	5 mM	0.5909 mL	2.9547 mL	5.9095 mL	14.7737 mL
	10 mM	0.2955 mL	1.4774 mL	2.9547 mL	7.3868 mL
	15 mM	0.1970 mL	0.9849 mL	1.9698 mL	4.9246 mL
	20 mM	0.1477 mL	0.7387 mL	1.4774 mL	3.6934 mL
	25 mM	0.1182 mL	0.5909 mL	1.1819 mL	2.9547 mL
	30 mM	0.0985 mL	0.4925 mL	0.9849 mL	2.4623 mL
	40 mM	0.0739 mL	0.3693 mL	0.7387 mL	1.8467 mL
	50 mM	0.0591 mL	0.2955 mL	0.5909 mL	1.4774 mL
	60 mM	0.0492 mL	0.2462 mL	0.4925 mL	1.2311 mL
	80 mM	0.0369 mL	0.1847 mL	0.3693 mL	0.9234 mL
	100 mM	0.0295 mL	0.1477 mL	0.2955 mL	0.7387 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Idebenone (Synonyms: 艾地苯醌)

Customer Review

Other Forms of Idebenone:

MCE 顾客使用本产品发表的 6 篇科研文献