2. Apoptosis
  3. Apoptosis
  4. Justicidin A

justicidin A 是一种天然产物，可以从 Justicia procumbens 中分离得到。justicidin A 降低 Ku70 的水平，导致 Bax 从胞质溶胶转移到线粒体以诱导细胞凋亡。justicidin A 可用于癌症研究。

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Justicidin A Chemical Structure

Justicidin A Chemical Structure

CAS No. : 25001-57-4

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规格 价格 是否有货 数量
1 mg ￥1080
In-stock
5 mg ￥2700
In-stock
10 mg ￥4300
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100 mg   询价  

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Customer Review

Justicidin A 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

生物活性

justicidin A is a nature product that could be isolated form Justicia procumbens. justicidin A decreases the level of Ku70 leading to translocation of Bax from the cytosol to mitochondria to induce apoptosis. justicidin A can be used in research of cancer[1].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
Ca-Ski ED50
3 ng/mL
Compound: 5
Cytotoxicity against human CaSki cells after 6 days by MTT assay
Cytotoxicity against human CaSki cells after 6 days by MTT assay
[PMID: 10425143]
Ca-Ski ED50
3 ng/mL
Compound: 1
Cytotoxicity against human CaSKi cells by MTT assay
Cytotoxicity against human CaSKi cells by MTT assay
[PMID: 11908984]
Hep 3B2 ED50
2.9 x 10-2 μg/mL
Compound: 1
Cytotoxicity against human Hep3B cells by MTT assay
Cytotoxicity against human Hep3B cells by MTT assay
[PMID: 11908984]
HepG2 IC50
163 μM
Compound: 2
Cytotoxicity against Homo sapiens (human) HepG2 cells after 3 days by MTT assay
Cytotoxicity against Homo sapiens (human) HepG2 cells after 3 days by MTT assay
10.1007/s00044-009-9172-1
HepG2 ED50
2 x 10-2 μg/mL
Compound: 1
Cytotoxicity against human HepG2 cells by MTT assay
Cytotoxicity against human HepG2 cells by MTT assay
[PMID: 11908984]
HT-3 IC50
0.0018 μg/mL
Compound: 110
Antiproliferative activity against human HT3 cells assessed as reduction in cell viability after 6 days by MTT assay
Antiproliferative activity against human HT3 cells assessed as reduction in cell viability after 6 days by MTT assay
[PMID: 30830783]
HT-3 ED50
1.8 ng/mL
Compound: 5
Cytotoxicity against human HT-3 cells after 6 days by MTT assay
Cytotoxicity against human HT-3 cells after 6 days by MTT assay
[PMID: 10425143]
MCF7 ED50
3.9 x 10-1 μg/mL
Compound: 1
Cytotoxicity against human MCF7 cells by MTT assay
Cytotoxicity against human MCF7 cells by MTT assay
[PMID: 11908984]
Platelet IC50
31.7 μM
Compound: 3
Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet-rich blood by turbidimetric method
Inhibition of arachidonic acid-induced platelet aggregation in rabbit platelet-rich blood by turbidimetric method
[PMID: 8988600]
PLC-PRF-5 ED50
2.2 ng/mL
Compound: 5
Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay
Cytotoxicity against human PLC/PRF/5 cells after 6 days by MTT assay
[PMID: 10425143]
SiHa ED50
7.4 ng/mL
Compound: 5
Cytotoxicity against human SiHa cells after 6 days by MTT assay
Cytotoxicity against human SiHa cells after 6 days by MTT assay
[PMID: 10425143]
SiHa ED50
7.4 ng/mL
Compound: 1
Cytotoxicity against human SiHa cells by MTT assay
Cytotoxicity against human SiHa cells by MTT assay
[PMID: 11908984]
T-24 ED50
2 ng/mL
Compound: 5
Cytotoxicity against human T24 cells after 6 days by MTT assay
Cytotoxicity against human T24 cells after 6 days by MTT assay
[PMID: 10425143]
体外研究
(In Vitro)

justicidin A (0-2.5 μM; 6 天) 抑制结直肠癌细胞的生长，对 HT-29、HCT 116、SiHa、MCF7 和 T24 细胞的 IC50 值分别为 0.110、0.400、0.020、1.540 和 0.004 μM[1]。
justicidin A (0.75 和 5 μM; 0-72 h) 诱导结直肠癌细胞凋亡[1]。
justicidin A (0.75 和 5 μM; 0-72 h) 诱导人结直肠癌细胞中 caspases 及其靶蛋白的裂解[1]。
justicidin A (0.75 和 5 μM; 0-72 h) 损伤线粒体，并从线粒体释放 cyto c 和 Smac[1]。
justicidin A (0.75 和 5 μM; 0-72 h) 降低 Ku70 水平，导致 Bax 从细胞质转位到线粒体[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Justicidin A 相关抗体:

Cell Viability Assay[1]

Cell Line: HT-29, HCT 116, SiHa, MCF7 and T24 cells
Concentration: 0-2.5 μM
Incubation Time: 6 days
Result: Inhibited cell growth in a dose-dependent manner.

Apoptosis Analysis[1]

Cell Line: HT-29 and HCT 116 cells
Concentration: 0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time: 0, 12, 16, 20, 24, 48, and 72 hours
Result: Induced apoptosis in a time-dependent manner.
Increased the population of sub-G 1 cells in a time-dependent manner.

Western Blot Analysis[1]

Cell Line: HT-29 and HCT 116 cells
Concentration: 0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time: 0, 12, 16, 20, 24, 48, 72, and 96 hours
Result: Decreased the amount of cytosolic Ku70 in HCT 116 cells and decreased (0.6-fold) at 48 h and reached the lowest level (0.33-fold) at 96 h.

Western Blot Analysis[1]

Cell Line: HT-29 and HCT 116 cells
Concentration: 0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time: 0, 12, 16, 20, 24, 48, and 72 hours
Result: Induced the cleavage of caspases and their target proteins.
体内研究
(In Vivo)

justicidin A (6.2 mg/kg; p.o.; 每天, 持续 56 天) 抑制小鼠中人类结直肠癌细胞的生长[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD-SCID mice with HT-29 cells xenograftes[1]
Dosage: 6.2 mg/kg
Administration: Oral administration; daily, for 56 days
Result: Inhibited tumor growth and decreased tumor weight.
分子量

394.37

Formula

C22H18O7
CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 16.67 mg/mL (42.27 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5357 mL 12.6784 mL 25.3569 mL
5 mM 0.5071 mL 2.5357 mL 5.0714 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

Justicidin A 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5357 mL 12.6784 mL 25.3569 mL 63.3922 mL
5 mM 0.5071 mL 2.5357 mL 5.0714 mL 12.6784 mL
10 mM 0.2536 mL 1.2678 mL 2.5357 mL 6.3392 mL
15 mM 0.1690 mL 0.8452 mL 1.6905 mL 4.2261 mL
20 mM 0.1268 mL 0.6339 mL 1.2678 mL 3.1696 mL
25 mM 0.1014 mL 0.5071 mL 1.0143 mL 2.5357 mL
30 mM 0.0845 mL 0.4226 mL 0.8452 mL 2.1131 mL
40 mM 0.0634 mL 0.3170 mL 0.6339 mL 1.5848 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Justicidin A25001-57-4Apoptosisnature productJusticia procumbensKu70BaxmitochondriacancerInhibitorinhibitorinhibit

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