2. Epigenetics Apoptosis Cell Cycle/DNA Damage
  3. DNA Methyltransferase Apoptosis Nucleoside Antimetabolite/Analog
  4. Decitabine

Decitabine  (Synonyms: 地西他滨; 5-Aza-2'-deoxycytidine; 5-AZA-CdR; NSC 127716)

目录号: HY-A0004 纯度: 99.87%
COA 产品使用指南

摩尔计算器

Decitabine (5-Aza-2'-deoxycytidine) 是一种具有口服活性的脱氧胞苷类似物抗代谢物 (deoxycytidine analogue antimetabolite) 和 DNA 甲基转移酶 (DNA methyltransferase) 抑制剂。Decitabine 代替 DNA 掺入胞嘧啶可以将 DNA 甲基转移酶共价捕获到 DNA 中，从而导致对该酶的不可逆抑制。Decitabine 诱导细胞 G2/M 阻滞和细胞凋亡 (apoptosis)，并具有有效的抗癌活性。

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Decitabine Chemical Structure

Decitabine Chemical Structure

CAS No. : 2353-33-5

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥716
In-stock
5 mg ￥406
In-stock
10 mg ￥651
In-stock
50 mg ￥1900
In-stock
100 mg ￥3000
In-stock
200 mg   询价  
500 mg   询价  

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Customer Review

Other Forms of Decitabine:

Decitabine 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 99 篇科研文献

WB
RT-PCR
Proliferation Assay
IF

    Decitabine purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807.  [Abstract]

    The protein level of GSDME in cells is quantitatively analysed by western blot in Y79 and WERI-RB-1 cells after Decitabine (2 nM; 3 days) treatment.

    Decitabine purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807.  [Abstract]

    The mRNA expression of GSDME is detected using RT-PCR after treatment with different concentrations of Decitabine for 3 days (the cells were centrifuged, and decitabine is replaced every day).

    Decitabine purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807.  [Abstract]

    The protein level of GSDME in cells is detected using immunofluorescence in Y79 and WERI-RB-1 cells after Decitabine (2 nM; 3 days) treatment.

    Decitabine purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2022 Mar 29;2022:2371807.  [Abstract]

    The toxicity of decitabine to Y79 and WERI-RB-1 cells is detected by CCK-8 assay at different concentrations of Decitabine.

    Decitabine purchased from MCE. Usage Cited in: Theranostics. 2021 Mar 20;11(11):5605-5619.  [Abstract]

    The mRNA levels of PCK2 in RCC cell lines after 5-AZA treatment. PCK2 protein expression in RCC cell lines after 5-AZA treatment.

    Decitabine purchased from MCE. Usage Cited in: Cell Prolif. 2021 May 24;e13072.  [Abstract]

    The indicated HSCs are subjected to DNMTs inhibitor (5‐azadC, 2 μM), G9a inhibitor (UNC0642, 2 μM) and pan‐HDAC inhibitor (ITF‐2357, 100 nM) for 48 h, or EZH2 inhibitor (EPZ‐6438, 10 μM) for 72h. KLF14 expression is measured by Western blotting analyses.

    Decitabine purchased from MCE. Usage Cited in: Cell Prolif. 2021 May 24;e13072.  [Abstract]

    The indicated HSCs are subjected to DNMTs inhibitor (5‐azadC, 2 μM), G9a inhibitor (UNC0642, 2 μM) and pan‐HDAC inhibitor (ITF‐2357, 100 nM) for 48 h, or EZH2 inhibitor (EPZ‐6438, 10 μM) for 72h. KLF14 expression is measured by RT‐qPCR.

    Decitabine purchased from MCE. Usage Cited in: Prog Neurobiol. 2021 Feb 23;102028.  [Abstract]

    The TF changes induced by DNMT3L in these cells are not affected with Decitabine (10 µM; for 24 hours) treatment in most genes tested, except NEUROD2 and NEUROG2 as shown by qRTPCR.

    Decitabine purchased from MCE. Usage Cited in: Oncogene. 2020 Aug;39(33):5507-5519.  [Abstract]

    Quantitative real-time PCR analysis of CDH13 expression after 5 μM Decitabine treatment.

    Decitabine purchased from MCE. Usage Cited in: J Invest Dermatol. 2020 Dec;140(12):2442-2454.e5.  [Abstract]

    In EZH2 mutant cells, after six days of drug treatment with both GSK126 and 0.5 μM Decitabine, Western blots shows complete elimination of H3K37me3 with reductions in DNMT1A, DNMT3A, and DNMT3B.

    Decitabine purchased from MCE. Usage Cited in: J Invest Dermatol. 2020 Dec;140(12):2442-2454.e5.  [Abstract]

    In EZH2 mutant cells, after six days of drug treatment with both GSK126 and 0.5 μM Decitabine, Significant upregulation of RASSF5, EPAS1, ITGB2, and NDRG1 target gene expression is seen in EZH2 mutant cell lines.

    Decitabine purchased from MCE. Usage Cited in: Ann Rheum Dis. 2019 Oct;78(10):1420-1429.  [Abstract]

    Western blot of PPARγ from mouse chondrocytes treated with IL-1β (10 ng/mL) for 24 hours in absence or presence 5Aza of various doses (0, 1, 5 and 10 µM).

    Decitabine purchased from MCE. Usage Cited in: Ann Rheum Dis. 2019 Oct;78(10):1420-1429.  [Abstract]

    Western blot of PPARγ, MMP13, Aamts5, Aggrecan and Collagen2 from Sham, DMM and 5Aza-treated mouse joint tissues ( (0.35 mg/kg; intraperitoneal injection daily; 10 weeks).

    Decitabine purchased from MCE. Usage Cited in: J Allergy Clin Immunol. 2019 Jun;143(6):2038-2051.e12.  [Abstract]

    Naive CD41 T cells are stimulated with anti-CD3/CD28 in the presence of mock stimulant or Decitabine (5-aza-29-deoxycytidine; 1 µM). Real-time PCR analysis of Il4 and Il13 mRNA after 24 hours of stimulation and IL-4 and IL-13 concentrations in culture supernatants after 72 hours of stimulation are measured.

    查看 DNA Methyltransferase 亚型特异性产品:

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    DNA Methyltransferase DNMT1 DNMT3 MGMT

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Decitabine (NSC 127716) is an orally active deoxycytidine analogue antimetabolite and a DNA methyltransferase inhibitor. Decitabine incorporates into DNA in place of cytosine can covalently trap DNA methyltransferase to DNA causing irreversible inhibition of the enzyme. Decitabine induces cell G2/M arrest and cell apoptosis. Decitabine has potent anticancer activity[1][2].

    IC50 & Target[5]

    DNMT1

     

    DNMT3A

     

    DNMT3B

     

    体外研究
    (In Vitro)

    在暴露于 Decitabine 后 96 小时，Decitabine 处理显著抑制 SNU719、NCC24 和 KATOIII 的细胞生长。Decitabine 在 EBVaGC 中诱导 G2/M 停滞和细胞凋亡，抑制侵袭能力，并上调 EBVaGC 的 E-钙粘蛋白表达[1]。
    只有高浓度 (10 μM) Decitabine (0.1-1 μM；24-72 小时) 导致 G2 期停滞，伴随着 G1 期细胞减少[3]。
    Decitabine 上调 DCTPP1 和 dUTPase 表达在 HeLa 细胞中[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Decitabine 相关抗体:

    Cell Cycle Analysis[1]

    Cell Line: HCT116 cells
    Concentration: 0.1, 1, 10 µM
    Incubation Time: 24, 48, 72 hours
    Result: Only high drug concentrations (10 µM) resulted in a G2 phase arrest, which was accompanied by a reduction of cells in G1 phase.
    体内研究
    (In Vivo)

    Decitabine (1.0 mg/kg，po) 与四氢尿苷 (THU) 联合使用会导致雌性 CD-1 小鼠发生严重毒性，并导致与 Decitabine 血浆水平相关的对 Decitabine 毒性的敏感性增加[5]。
    Decitabine (1.0 mg/kg；腹腔注射；每天一次，连续 5 天) 导致 C57BL/6 小鼠中建立的 EL4 肿瘤消退[7]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6 mice (bearing EL4 cells)[6]
    Dosage: 1.0 mg/kg
    Administration: Intraperitoneal injection; once daily for 5 consecutive days
    Result: Caused continuous tumor regression even after Decitabine treatment was stopped.
    Clinical Trial
    分子量

    228.21

    Formula

    C8H12N4O4
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    地西他滨；地西他宾；2'-脱氧-5-氮杂胞啶；2'-脱氧-5-氮杂胞啶
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, protect from light

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : ≥ 50 mg/mL (219.10 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 20 mg/mL (87.64 mM; 超声助溶)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 4.3819 mL 21.9096 mL 43.8193 mL
    5 mM 0.8764 mL 4.3819 mL 8.7639 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.95 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (10.95 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 10 mg/mL (43.82 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.97%

    COA (290 KB) HNMR (199 KB) LCMS (347 KB)

    产品使用指南 (1538 KB)
    参考文献

    Decitabine 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.3819 mL 21.9096 mL 43.8193 mL 109.5482 mL
    5 mM 0.8764 mL 4.3819 mL 8.7639 mL 21.9096 mL
    10 mM 0.4382 mL 2.1910 mL 4.3819 mL 10.9548 mL
    15 mM 0.2921 mL 1.4606 mL 2.9213 mL 7.3032 mL
    20 mM 0.2191 mL 1.0955 mL 2.1910 mL 5.4774 mL
    25 mM 0.1753 mL 0.8764 mL 1.7528 mL 4.3819 mL
    30 mM 0.1461 mL 0.7303 mL 1.4606 mL 3.6516 mL
    40 mM 0.1095 mL 0.5477 mL 1.0955 mL 2.7387 mL
    50 mM 0.0876 mL 0.4382 mL 0.8764 mL 2.1910 mL
    60 mM 0.0730 mL 0.3652 mL 0.7303 mL 1.8258 mL
    80 mM 0.0548 mL 0.2739 mL 0.5477 mL 1.3694 mL
    DMSO 100 mM 0.0438 mL 0.2191 mL 0.4382 mL 1.0955 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Decitabine2353-33-55-Aza-2'-deoxycytidine 5-AZA-CdR NSC 127716地西他滨地西他宾2'-脱氧-5-氮杂胞啶NSC127716NSC 127716NSC-127716DNA MethyltransferaseApoptosisNucleoside Antimetabolite/AnalogDNMTsDNA MTasesInhibitorinhibitorinhibit

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