2. Cell Cycle/DNA Damage Anti-infection Apoptosis Metabolic Enzyme/Protease
  3. Nucleoside Antimetabolite/Analog HIV Apoptosis Endogenous Metabolite
  4. 5-Fluorouracil

5-Fluorouracil  (Synonyms: 5-氟脲嘧啶; 5-FU)

目录号: HY-90006 纯度: 99.99%
COA 产品使用指南

摩尔计算器

5-Fluorouracil (5-FU) 是一种尿嘧啶的类似物 (nucleoside antimetabolite/analog)，是一种有效的抗肿瘤药。5-Fluorouracil 通过抑制胸苷酸合成酶影响嘧啶的合成，从而耗尽细胞内dTTP 池。5-Fluorouracil 诱导细胞凋亡 (apoptosis)，可用作化学敏化剂。5-Fluorouracil 还可抑制 HIV 病毒。5-Fluorouracil (5-FU) 可破坏外泌体特异性的 rRNA。

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5-Fluorouracil Chemical Structure

5-Fluorouracil Chemical Structure

CAS No. : 51-21-8

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥500
In-stock
50 mg ￥140
In-stock
200 mg ￥350
In-stock
1 g ￥450
In-stock
5 g ￥840
In-stock
10 g   询价  
50 g   询价  

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Customer Review

Other Forms of 5-Fluorouracil:

5-Fluorouracil 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 199 篇科研文献

WB
IF
Proliferation Assay
IHC
RT-PCR

    5-Fluorouracil purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2022 Oct;9(30):e2200717.  [Abstract]

    A transient upregulation and subsequent downregulation of RSK2 phosphorylation at Thr577 by 5-Fluorouracil (5-FU), which is accompanied by the accumulation of γ‐H2AX phosphorylation in a time‐dependent manner.

    5-Fluorouracil purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2022 Oct;9(30):e2200717.  [Abstract]

    In Hct116 cells, the inhibitory effects of 5-Fluorouracil (5-FU on cell growth at 2-10 μM are significantly enhanced by RSK2 or PAK4 inhibitors in short‐term and long‐term assays.

    5-Fluorouracil purchased from MCE. Usage Cited in: J Immunother Cancer. 2022 Aug;10(8):e004006.  [Abstract]

    Hep3B and Huh7 cells are treated with 5-Fluorouracil for 24 h, then the cellular protein expression of p-IRF3 and pP65 is detected by immunoblotting.

    5-Fluorouracil purchased from MCE. Usage Cited in: Int J Biol Sci. 2022 Apr 11;18(7):2882-2897.  [Abstract]

    Representative immunoblots assessing apoptosis induced by 5-Fluorouracil (5-FU) in immortalized human ventricular myocytes (AC16).

    5-Fluorouracil purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936.  [Abstract]

    The combination of SR140333 or aprepitant and 5-Fluorouracil (5-FU; mg/kg) decreases the expression levels of ABCC1, ABCG2, p-ERK1/2, and c-Myc and increased those of p-PERK, ATF4, and CHOP compared with 5-FU treatment alone.

    5-Fluorouracil purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936.  [Abstract]

    Cell viability upon treatment with 5-Fluorouracil (5-FU; 60 μM), SR140333, or Aprepitant either as single agents or in combination at 24 h in HCT116 cells.

    5-Fluorouracil purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936.  [Abstract]

    ER stress pathways in HCT116/5-FU cells treated with 5-Fluorouracil (5-FU; 60 μM) with SR140333 or Aprepitant either as single agents or in combination at 24 h.

    5-Fluorouracil purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936.  [Abstract]

    Western blotting of drug-resistant proteins in HCT116/5-FU cells treated with 5-Fluorouracil (5-FU; 80 μM) at 48 h.

    5-Fluorouracil purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2021 Apr 30;40(1):150.  [Abstract]

    Inhibition rate of proliferation in pcDNA3.1 or ZBTB28 transfected cells treated with gradient concentration of 5-FU for 72 hours.

    5-Fluorouracil purchased from MCE. Usage Cited in: Proc Natl Acad Sci U S A. 2021 Jul 20;118(29):e2026813118.  [Abstract]

    Overexpression of RMRP impairs 5-FU–induced p53 activation determined by RT-qPCR.

    5-Fluorouracil purchased from MCE. Usage Cited in: Proc Natl Acad Sci U S A. 2021 Jul 20;118(29):e2026813118.  [Abstract]

    Overexpression of RMRP impairs 5-FU-induced p53 activation determined by IB.

    5-Fluorouracil purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Apr 1;12(4):338.  [Abstract]

    SW620, CACO2, and HCT15 cells are treated with different concentrations of UTD1 and 5-Fluorouracil (5-FU), and cell viability is measured by CCK-8.

    5-Fluorouracil purchased from MCE. Usage Cited in: Int J Biochem Cell Biol. 2018 Mar;96:9-19.  [Abstract]

    Hep3B cells are treated with 2 μmol Shikonin, 5-FU, BSNQ or OSNQ for different times (3, 6, 12 and 24 h), and stained with Annexin V and PI. The apoptotic cells are detected by fluorescence microscopy and quantified as percentages.

    5-Fluorouracil purchased from MCE. Usage Cited in: J Cell Biochem. 2018 Nov 28.  [Abstract]

    MKN45 and MKN28 cells transfected with empty and DEC2 vectors are incubated in control medium or 5-Fu of 10 μM for 48 hours, then proliferation ratio of these cells is figured by EdU incorporation assay.

    5-Fluorouracil purchased from MCE. Usage Cited in: J Cell Biochem. 2018 Nov 28.  [Abstract]

    The expressions of apoptotic-related proteins, cleaved-PARP, caspase-3-active are detected in control, STAT5A, STAT5A+5-Fu, DEC2+5-Fu, DEC2/STAT5A+5-Fu of MKN45 and MKN28 cell lines using Western blot analysis.

    5-Fluorouracil purchased from MCE. Usage Cited in: J Cell Biochem. 2018 Nov 28.  [Abstract]

    The expressions of apoptotic-related proteins, cleaved-PARP, caspase-3-active and survivin are detected in MKN45, MKN45+5-Fu, MKN45-EV, MKN45-DEC2, MKN45-DEC2+5-Fu, MKN28, MKN28+5-Fu, MKN28-EV, MKN28-DEC2, MKN28-DEC2+5-Fu cells using Western blot analysis.

    5-Fluorouracil purchased from MCE. Usage Cited in: Med Sci Monit. 2018 Sep 21;24:6666-6672.  [Abstract]

    Expression of protein of OPA1, Mfn1, Mfn2, Drp1 and Fis1 in heart mitochondria of rats treated with different 5-FU concentrations. The control group is intraperitonealled injection with same amount of physiological saline.

    5-Fluorouracil purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Jun 27;501(3):636-642.  [Abstract]

    Immunoblot analysis of the expression levels of BCL-2 and BAX after treatment of HCT116 TP53-/- cells with 20 μM 5-FU and 1.25 μM DHA alone or in combination for 48 h. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as a loading control.

    5-Fluorouracil purchased from MCE. Usage Cited in: Tumour Biol. 2016 Jul;37(7):8811-24.  [Abstract]

    Assays for autophagy activity. Immunoblot analysis for LC3B. Compared with the Lenti-NC cells, the Lenti-CD44v6 cells display a higher intensity of autophagy flux but not steady-state levels when treated with 5-FU (*P<0.001).

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    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    5-Fluorouracil (5-FU) is an analogue of uracil and a potent antitumor agent. 5-Fluorouracil affects pyrimidine synthesis by inhibiting thymidylate synthetase thus depleting intracellular dTTP pools. 5-Fluorouracil induces apoptosis and can be used as a chemical sensitizer[1][2]. 5-Fluorouracil also inhibits HIV[3].

    IC50 & Target[1][6]

    HIV

     

    体外研究
    (In Vitro)

    5-Fluorouracil (5-Fu) 和 NSC 123127 (Dox) 具有协同抗癌功效。5-Fu/Dox-DNM 对人乳腺癌 (MDA-MB-231) 细胞的 IC50 值为 0.25 μg/mL，细胞毒性分别增加 11.2 倍和 6.1 倍分别为 Dox-DNM 和 5-Fu-DNM[1]。在 5-Fluorouracil (5-FU) 和 CDDP 处理的 NFBD1 抑制 NPC 细胞中，使用慢病毒介导的短发夹 RNA 耗尽 NPC CNE1 细胞系中的 NFBD1 表达，并且这些细胞的敏感性升高。NFBD1 敲低导致 CDDP 或 5-FU 处理的 CNE1 细胞明显诱导细胞凋亡[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    5-Fluorouracil 相关抗体:
    体内研究
    (In Vivo)

    5-Fluorouracil (23 mg/kg，每周 3 次) 持续 14 天，在处理开始后第 3 天诱导与急性肠道炎症相关的胃肠道传输加速，这可能导致第 7 天后观察到的 ENS 持续变化和 14 项处理导致胃肠道传输延迟和结肠运动障碍[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    130.08

    Formula

    C4H3FN2O2
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    5-氟脲嘧啶；双喃呋啶
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 50 mg/mL (384.38 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    1 M Ammonium hydroxide 中的溶解度 : 50 mg/mL (384.38 mM; 超声助溶)

    H2O 中的溶解度 : 10 mg/mL (76.88 mM; 超声助溶 (<60°C))

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 7.6876 mL 38.4379 mL 76.8758 mL
    5 mM 1.5375 mL 7.6876 mL 15.3752 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (19.22 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (19.22 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 25 mg/mL (192.19 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.99%

    COA (292 KB) RP-HPLC (258 KB) 元素分析报告 (216 KB)

    产品使用指南 (1538 KB)
    参考文献
    Animal Administration
    [2]

    Mice receive intraperitoneal injections of 5-FU (23 mg/kg), 3 times a week via a 26 gauge needle. 5-FU is dissolved in 100% dimethyl sulfoxide (DMSO) to make 1 M/L stock solution refrigerated at −20°C. The stock is then defrosted and diluted with sterile water to make 0.1 M/L (10% DMSO) solutions for intraperitoneal injections. The dose of 5-FU is calculated to be equivalent to standard human dose per body surface area. The low doses of 5-FU (10-40 mg/kg) have been shown to have antitumor efficacy in mouse models of cancer. Sham-treated mice received 10% DMSO in sterile water via intraperitoneal injection three times a week via a 26 gauge needle. The injected volumes are calculated to the body weight; the maximum volume does not exceed 200 μL per injection. Mice are euthanized via cervical dislocation at 3 (2 treatments), 7 (3 treatments), and 14 (6 treatments) days after the first injection and colon is collected for in vitro experiments.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    5-Fluorouracil 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO / 1 M Ammonium hydroxide 1 mM 7.6876 mL 38.4379 mL 76.8758 mL 192.1894 mL
    5 mM 1.5375 mL 7.6876 mL 15.3752 mL 38.4379 mL
    10 mM 0.7688 mL 3.8438 mL 7.6876 mL 19.2189 mL
    15 mM 0.5125 mL 2.5625 mL 5.1251 mL 12.8126 mL
    20 mM 0.3844 mL 1.9219 mL 3.8438 mL 9.6095 mL
    25 mM 0.3075 mL 1.5375 mL 3.0750 mL 7.6876 mL
    30 mM 0.2563 mL 1.2813 mL 2.5625 mL 6.4063 mL
    40 mM 0.1922 mL 0.9609 mL 1.9219 mL 4.8047 mL
    50 mM 0.1538 mL 0.7688 mL 1.5375 mL 3.8438 mL
    60 mM 0.1281 mL 0.6406 mL 1.2813 mL 3.2032 mL
    DMSO / 1 M Ammonium hydroxide 80 mM 0.0961 mL 0.4805 mL 0.9609 mL 2.4024 mL
    100 mM 0.0769 mL 0.3844 mL 0.7688 mL 1.9219 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    5-Fluorouracil51-21-85-FU5-氟脲嘧啶双喃呋啶Nucleoside Antimetabolite/AnalogHIVApoptosisEndogenous MetaboliteHuman immunodeficiency virusInhibitorinhibitorinhibit

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