1. Neuronal Signaling Apoptosis
  2. Cholinesterase (ChE) Apoptosis
  3. Scopoletin

Scopoletin  (Synonyms: 东莨菪内酯; Gelseminic acid; Chrysatropic acid)

目录号: HY-N0342 纯度: 99.89%
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Scopoletin 是一种乙酰胆碱酯酶 (AChE) 抑制剂。

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Scopoletin Chemical Structure

Scopoletin Chemical Structure

CAS No. : 92-61-5

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Other Forms of Scopoletin:

查看 Cholinesterase (ChE) 亚型特异性产品:

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Scopoletin is an inhibitor of acetylcholinesterase (AChE).

IC50 & Target

AChE

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A-431 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human A431 cells after 24 hrs by MTT assay
Cytotoxicity against human A431 cells after 24 hrs by MTT assay
[PMID: 23218780]
A549 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human A549 cells after 24 hrs by MTT assay
Cytotoxicity against human A549 cells after 24 hrs by MTT assay
[PMID: 23218780]
A549 IC50
> 25 μM
Compound: Scopoletin
Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
[PMID: 32438251]
A549 IC50
> 30 μM
Compound: 6, Scopoletin
Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 25703298]
BXPC-3 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human BxPC3 cells after 24 hrs by MTT assay
Cytotoxicity against human BxPC3 cells after 24 hrs by MTT assay
[PMID: 23218780]
HCT-116 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay
Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay
[PMID: 23218780]
HCT-116 IC50
> 25 μM
Compound: Scopoletin
Growth inhibition of human HCT116 cells after 48 hrs by MTT assay
Growth inhibition of human HCT116 cells after 48 hrs by MTT assay
[PMID: 27956344]
HCT-116 IC50
> 30 μM
Compound: 6, Scopoletin
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 25703298]
HeLa IC50
> 80 μM
Compound: Scopoletin
Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
[PMID: 36242987]
HepG2 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay
Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay
[PMID: 23218780]
HepG2 IC50
> 25 μM
Compound: Scopoletin
Anticancer activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
Anticancer activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
[PMID: 32438251]
HL-60 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human HL60 cells after 24 hrs by MTT assay
Cytotoxicity against human HL60 cells after 24 hrs by MTT assay
[PMID: 23218780]
HL-60 IC50
9.5 μM
Compound: 3
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
[PMID: 15387667]
HT-1080 IC50
12.5 μM
Compound: 3
Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
[PMID: 15387667]
HT-29 IC50
> 100 μM
Compound: 7, scopoletin
Antiproliferative activity against human HT-29 cells after 72 hrs by crystal violet staining method
Antiproliferative activity against human HT-29 cells after 72 hrs by crystal violet staining method
[PMID: 22765897]
Huh-7 IC50
> 25 μM
Compound: Scopoletin
Growth inhibition of human HuH7 cells after 48 hrs by MTT assay
Growth inhibition of human HuH7 cells after 48 hrs by MTT assay
[PMID: 27956344]
HuT78 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human HUT78 cells after 24 hrs by MTT assay
Cytotoxicity against human HUT78 cells after 24 hrs by MTT assay
[PMID: 23218780]
K562 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human K562 cells after 24 hrs by MTT assay
Cytotoxicity against human K562 cells after 24 hrs by MTT assay
[PMID: 23218780]
L02 IC50
> 25 μM
Compound: Scopoletin
Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
[PMID: 32438251]
L6 IC50
> 90 μM
Compound: 4
Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorimetry
Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorimetry
[PMID: 21438586]
LoVo IC50
18.5 μM
Compound: 3
Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay
Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay
[PMID: 15387667]
LoVo IC50
7.3 μM
Compound: 3
Cytotoxicity against human LoVo cells after 72 hrs by MTT assay
Cytotoxicity against human LoVo cells after 72 hrs by MTT assay
[PMID: 15387667]
Macrophage IC50
5 μM
Compound: 4, scopoletin
Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method
Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method
[PMID: 8988605]
MCF-10A IC50
> 25 μM
Compound: Scopoletin
Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
[PMID: 34315044]
MCF7 IC50
> 100 μM
Compound: 7, scopoletin
Antiproliferative activity against human MCF7 cells after 72 hrs by crystal violet staining method
Antiproliferative activity against human MCF7 cells after 72 hrs by crystal violet staining method
[PMID: 22765897]
MCF7 IC50
> 25 μM
Compound: Scopoletin
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
[PMID: 34315044]
MCF7 IC50
> 25 μM
Compound: Scopoletin
Anticancer activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
Anticancer activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay
[PMID: 32438251]
MCF7 IC50
> 80 μM
Compound: Scopoletin
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
[PMID: 36242987]
MDA-MB-231 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay
[PMID: 23218780]
MDA-MB-231 IC50
> 100 μM
Compound: 7, scopoletin
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by crystal violet staining method
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by crystal violet staining method
[PMID: 22765897]
MDA-MB-231 IC50
> 25 μM
Compound: Scopoletin
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
[PMID: 34315044]
MDA-MB-231 IC50
> 30 μM
Compound: 6, Scopoletin
Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 25703298]
MDA-MB-435 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human MDA-MB-435 cells after 24 hrs by MTT assay
Cytotoxicity against human MDA-MB-435 cells after 24 hrs by MTT assay
[PMID: 23218780]
MGC-803 IC50
> 80 μM
Compound: Scopoletin
Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
[PMID: 36242987]
MKN-45 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human MKN45 cells after 24 hrs by MTT assay
Cytotoxicity against human MKN45 cells after 24 hrs by MTT assay
[PMID: 23218780]
PANC-1 IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human PANC1 cells after 24 hrs by MTT assay
Cytotoxicity against human PANC1 cells after 24 hrs by MTT assay
[PMID: 23218780]
PBMC IC50
> 100 μM
Compound: Sco, Scopoletin
Cytotoxicity against human PBMC after 24 hrs by MTT assay
Cytotoxicity against human PBMC after 24 hrs by MTT assay
[PMID: 23218780]
PC-3 IC50
72.19 μM
Compound: Scopoletin
Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
[PMID: 36242987]
Platelet IC50
> 50 μg/mL
Compound: Scopolatin
Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry
Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry
[PMID: 21106454]
Platelet IC50
41.74 μg/mL
Compound: Scopolatin
Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry
Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry
[PMID: 21106454]
Sf21 IC50
> 100 μM
Compound: 7
Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay
Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay
[PMID: 17378609]
SK-MEL-28 IC50
3.43 μM
Compound: 2
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 37197457]
SK-MEL3 IC50
1.5 μM
Compound: 2
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 37197457]
SW-620 IC50
> 25 μM
Compound: Scopoletin
Growth inhibition of human SW620 cells after 48 hrs by MTT assay
Growth inhibition of human SW620 cells after 48 hrs by MTT assay
[PMID: 27956344]
U-937 CC50
> 2000 μM
Compound: 8
Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay
Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay
[PMID: 22925447]
U-937 IC50
151.5 μM
Compound: 3
Growth inhibition of human U937 cells by [3H]thymidine incorporation assay
Growth inhibition of human U937 cells by [3H]thymidine incorporation assay
[PMID: 18060791]
U-937 CC50
600.2 μM
Compound: 3
Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs
Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs
[PMID: 18060791]
U-937 IC50
975.9 μM
Compound: 8
Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting
Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting
[PMID: 22925447]
体外研究
(In Vitro)

Scopoletin (SCT) 被确定为乙酰胆碱酯酶 (AChE) 的推定抑制剂。Scopoletin 增强 K+ 刺激的大鼠额叶皮质突触体 ACh 释放,显示出钟形剂量效应曲线 (Emax:4 μM)[1]。Scopoletin 通过诱导 PC3 细胞凋亡来抑制 PC3 增殖。Scopoletin抑制PC3、PAA (人肺癌细胞)、Hela 细胞增殖的 IC50 分别为 (157±25)、(154±51)、(294±100) mg/L。Scopoletin 诱导显著的时间和浓度依赖性 PC3 细胞增殖抑制。Scopoletin 以浓度依赖性方式降低 PC3 细胞中的蛋白质含量和酸性磷酸酶活性 (ACP) 水平。光镜、荧光显微镜、透射电镜观察 Scopoletin 处理后的细胞,显示出典型的细胞凋亡形态学变化。Scopoletin 0、100、200、400 mg/L 时细胞凋亡率分别为 0.3%、2.1%、9.3%、35%,东莨菪素处理后 G2 期细胞明显减少[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Scopoletin (2 μg,icv) 增加 T 迷宫交替并改善东莨菪碱诱导的胆碱能缺陷小鼠的新物体识别。 Scopoletin 还可以减少 15-18 个月大的小鼠(2 mg/kg sc)的对象记忆中与年龄相关的缺陷。 注射 2 μg Scopoletin 的小鼠表现出 71.3±2.5% 的交替率增加[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

192.17

Formula

C10H8O4

CAS 号
性状

固体

颜色

White to yellow

中文名称

东莨菪内酯;莨菪亭;东莨菪亭

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验: 

DMSO 中的溶解度 : 62.5 mg/mL (325.23 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

Ethanol 中的溶解度 : < 1 mg/mL (insoluble)

*Scopoletin is usually formulated as a suspension.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.2037 mL 26.0186 mL 52.0373 mL
5 mM 1.0407 mL 5.2037 mL 10.4075 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (10.82 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (10.82 mM); 悬浊液; 超声助溶

    此方案可获得 2.08 mg/mL的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

    2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.89%

参考文献
Cell Assay
[2]

PC3 cells (5×107/L) 1 mL in exponential growth are seeded into four 24-well plates. The plates are incubated at 37°C in a humidified 5% CO2 atmospbee. After 24h, Scopoletin 33, 66, 133, 266, and 533 mg/L are added to wells (3 wells for each concentration for each plate). For control cells (3 wells foreach plate),only DMEM was added. The plates are incubated continually. The viable cells are counted by hemocytometer every day in the frist 4 d by Trypan blue dye exclusion method[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
C56BL/6N male, 4-6-month-old and 16-18-month-old mice are used in the behavioral studies. The mice are housed in groups of four in cages at constant humidity (50-55%) and temperature (22±1°C) on a 12:12 h light/dark cycle (7:00–19:00 h), with food and water ad libitum. Younger mice (4-6 months) are implanted with i.c.v. cannulas for application of Scopolamine (SCOP) and Scopoletin. The aged mice are injected with Scopoletin by the s.c. route. Experiments are conducted between 8:00 and 16:00 h. Mice with i.c.v. cannulas are randomly divided into four experimental groups: vehicle; SCOP 20 μg; Scopoletin 2 μg; and SCOP 20 μg plus Scopoletin 2 μg. The drugs are applied in 1 μL of vehicle solution (SCOP: saline, Scopoletin: 3 DMSO: 7 sterile water). I.c.v. injections are carried out 15 min before the start of the tests. Aged mice obtained Scopoletin s.c. 30 min prior to object memory test (vehicle: 1 DMSO: 1 EtOH, diluted with olive oil as required)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.2037 mL 26.0186 mL 52.0373 mL 130.0931 mL
5 mM 1.0407 mL 5.2037 mL 10.4075 mL 26.0186 mL
10 mM 0.5204 mL 2.6019 mL 5.2037 mL 13.0093 mL
15 mM 0.3469 mL 1.7346 mL 3.4692 mL 8.6729 mL
20 mM 0.2602 mL 1.3009 mL 2.6019 mL 6.5047 mL
25 mM 0.2081 mL 1.0407 mL 2.0815 mL 5.2037 mL
30 mM 0.1735 mL 0.8673 mL 1.7346 mL 4.3364 mL
40 mM 0.1301 mL 0.6505 mL 1.3009 mL 3.2523 mL
50 mM 0.1041 mL 0.5204 mL 1.0407 mL 2.6019 mL
60 mM 0.0867 mL 0.4336 mL 0.8673 mL 2.1682 mL
80 mM 0.0650 mL 0.3252 mL 0.6505 mL 1.6262 mL
100 mM 0.0520 mL 0.2602 mL 0.5204 mL 1.3009 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Scopoletin
目录号:
HY-N0342
需求量: