Verbascoside (Synonyms: 麦角甾苷; Acteoside; Kusaginin; TJC160)

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Data Sheet SDS COA 产品使用指南

摩尔计算器

Verbascoside 是从 Acanthus mollis 中得到的糖苷类物质，为 ATP 竞争性的 PKC 抑制剂，IC₅₀ 值为 25 μM，具有抗肿瘤，抗炎，抗神经性疼痛等作用。

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Verbascoside Chemical Structure

CAS No. : 61276-17-3

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥550	In-stock
5 mg	￥400	In-stock
10 mg	￥650	In-stock
50 mg	￥2300	In-stock
100 mg	￥3695	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

Other Forms of Verbascoside:

Verbascoside (Standard) 询价

MCE 顾客使用本产品发表的 10 篇科研文献

Verbascoside 相关产品

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Verbascoside is isolated from Acanthus mollis, acts as an ATP-competitive inhibitor of PKC, with an IC₅₀ of 25 μM, and has antitumor, anti-inflammatory and antineuropathic pain activity.

IC₅₀ & Target^[1]

PKC

25 μM (IC₅₀)

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	5.81 μM Compound: 2	Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay	[PMID: 25981688]
Erythrocyte	IC₅₀	28 μM Compound: 6	Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs	[PMID: 12828473]
HCT-15	IC₅₀	2.73 μM Compound: 2	Cytotoxicity against human HCT15 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay Cytotoxicity against human HCT15 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay	[PMID: 25981688]
HEp-2	EC₅₀	0.8 μg/mL Compound: 1, verbascoside	Antiviral activity against RSV long infected in human Hep2 cells assessed as virus-induced cytopathic effect after 3 days Antiviral activity against RSV long infected in human Hep2 cells assessed as virus-induced cytopathic effect after 3 days	[PMID: 9599250]
HEp-2	IC₅₀	44 μg/mL Compound: 1, verbascoside	Cytotoxicity against mCMV RM-461 infected human Hep2 cells after 3 days Cytotoxicity against mCMV RM-461 infected human Hep2 cells after 3 days	[PMID: 9599250]
HEp-2	IC₅₀	71 μg/mL Compound: 1, verbascoside	Cytotoxicity against HSV2 infected human Hep2 cells after 3 days Cytotoxicity against HSV2 infected human Hep2 cells after 3 days	[PMID: 9599250]
HEp-2	EC₅₀	76.9 μg/mL Compound: 1, verbascoside	Cytotoxicity against RSV long infected human Hep2 cells after 3 days Cytotoxicity against RSV long infected human Hep2 cells after 3 days	[PMID: 9599250]
HEp-2	EC₅₀	9.7 μg/mL Compound: 1, verbascoside	Antiviral activity against RSV A-2 infected in human Hep2 cells after 3 days by plaque neutralization assay Antiviral activity against RSV A-2 infected in human Hep2 cells after 3 days by plaque neutralization assay	[PMID: 9599250]
Hepatocyte	IC₅₀	4.6 μM Compound: 5	Hepatoprotective activity in ddY mouse primary cultured hepatocytes assessed as inhibition of D-galactosamine-induced cytotoxicity after 44 hrs by MTT assay Hepatoprotective activity in ddY mouse primary cultured hepatocytes assessed as inhibition of D-galactosamine-induced cytotoxicity after 44 hrs by MTT assay	[PMID: 20159656]
J774.A1	EC₅₀	4.9 x 10^-4 M Compound: 2	Cytotoxicity against mouse J774A1 macrophages grown in monolayers by MTT assay Cytotoxicity against mouse J774A1 macrophages grown in monolayers by MTT assay	[PMID: 16309308]
L1210	IC₅₀	13 μM Compound: 1	Antiproliferative activity against mouse L1210 cells after 48 hrs by coulter counting analysis Antiproliferative activity against mouse L1210 cells after 48 hrs by coulter counting analysis	[PMID: 1812212]
L929	IC₅₀	17.8 μg/mL Compound: 5	Cytoprotective activity in mouse L929 cells assessed as inhibition of TNF-alpha-induced cytotoxicity after 44 hrs by MTT assay Cytoprotective activity in mouse L929 cells assessed as inhibition of TNF-alpha-induced cytotoxicity after 44 hrs by MTT assay	[PMID: 20159656]
SK-MEL-2	IC₅₀	8.52 μM Compound: 2	Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay	[PMID: 25981688]
SK-OV-3	IC₅₀	7.61 μM Compound: 2	Cytotoxicity against human SKOV3 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay Cytotoxicity against human SKOV3 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay	[PMID: 25981688]

体外研究
(In Vitro)

Verbascoside 是一种 ATP 竞争性 PKC 抑制剂，其 IC₅₀ 为 25 μM。Verbascoside 对 ATP 和组蛋白的 K_i 分别为 22 μM 和 28 μM。Verbascoside 对 L-1210 细胞具有较强的抗肿瘤活性，其 IC₅₀ 为 13 μM^[1]。Verbascoside (5 μM 和 10 μM) 抑制 2,4-二硝基氯苯 (DNCB) 诱导的 THP-1 细胞 T 细胞共刺激因子 CD86 和 CD54、促炎细胞因子及 NF-κB 信号通路的激活^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Verbascoside (1%) 可降低 2,4-二硝基氯苯 (DNCB) 诱发的特应性皮炎 (AD) 小鼠模型中总体抓挠行为发生率以及皮肤病变严重程度。Verbascoside 还可阻断 DNCB 诱导的皮肤病变中促炎细胞因子 TNF-α、IL-6 和 IL-4 mRNA 表达^[2]。Verbascoside (50、100 mg/kg，腹腔注射) 不会改变慢性压迫性损伤 (CCI) 诱发的冷异常性疼痛。Verbascoside (200 mg/kg，腹腔注射) 在第 3 天降低大鼠对冷刺激丙酮的超敏反应。Verbascoside 还可显著减少与神经病变相关的行为变化。此外，Verbascoside 在第 3 天降低 Bax 并增加 Bcl-2^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

624.59

Formula

C₂₉H₃₆O₁₅

CAS 号

61276-17-3

性状

固体

颜色

White to yellow

中文名称

毛蕊花糖苷；毛蕊花甙；麦角甾苷；类叶升麻苷

结构分类

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

细胞实验：

H₂O 中的溶解度 : ≥ 100 mg/mL (160.11 mM)

DMSO 中的溶解度 : ≥ 100 mg/mL (160.11 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.6011 mL	8.0053 mL	16.0105 mL
5 mM	0.3202 mL	1.6011 mL	3.2021 mL
10 mM	0.1601 mL	0.8005 mL	1.6011 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.33 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.33 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.33 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.83%

选择批次：

Data Sheet (649 KB) SDS (393 KB)

COA (268 KB) HNMR (345 KB) LCMS (99 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Herbert JM, et al. Verbascoside isolated from Lantana camara, an inhibitor of protein kinase C. J Nat Prod. 1991 Nov-Dec;54(6):1595-600. [Content Brief]

[2]. Li Y, et al. Verbascoside Alleviates Atopic Dermatitis-Like Symptoms in Mice via Its Potent Anti-Inflammatory Effect. Int Arch Allergy Immunol. 2018;175(4):220-230. [Content Brief]

[3]. Amin B, et al. The Effect of Verbascoside in Neuropathic Pain Induced by Chronic Constriction Injury in Rats. Phytother Res. 2016 Jan;30(1):128-35. [Content Brief]

Cell Assay ^[1]	The lymphocytic mouse leukemia L1210 cells (ATCC, CCL 219) are plated sparsely at 10⁴ cells per well in 24-well cluster plates in Dulbecco’s modified Eagle medium containing 10% fetal calf serum, 4 mM glutamine, 100 U/mL penicillin, 100 µg/mL streptomycin sulfate, and Verbascoside (solubilized in DMSO). After a 2-day incubation period at 37°C in a humidified atmosphere (5% CO₂ in air), growth is monitored by counting cell numbers in a Coulter-counter. IC₅₀ values are calculated on the basis of the linear regression lines established for each compound tested^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Rats^[2] To induce atopic dermatitis (AD)-like symptoms, 2,4-dinitrochlorobenzene (DNCB) is used. Briefly, the dorsal hair of the mice is removed using an electronic clipper 2 days before DNCB treatment. An application of 200 µL of 1% DNCB (in acetone:olive oil = 4:1) is made to the shaved dorsal skin for sensitization. The repeated challenge is performed on the same site with 0.2% DNCB once every 3 days for about 2 weeks. The mice are divided into 4 groups (n = 6 per group): (1) vehicle-treated controls, (2) DNCB-treated only, (3) 1% Verbascoside (in acetone:olive oil 4:1)-treated only, and (4) DNCB + 1% Verbascoside-treated group^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Herbert JM, et al. Verbascoside isolated from Lantana camara, an inhibitor of protein kinase C. J Nat Prod. 1991 Nov-Dec;54(6):1595-600. [Content Brief] [2]. Li Y, et al. Verbascoside Alleviates Atopic Dermatitis-Like Symptoms in Mice via Its Potent Anti-Inflammatory Effect. Int Arch Allergy Immunol. 2018;175(4):220-230. [Content Brief] [3]. Amin B, et al. The Effect of Verbascoside in Neuropathic Pain Induced by Chronic Constriction Injury in Rats. Phytother Res. 2016 Jan;30(1):128-35. [Content Brief]

Verbascoside 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.6011 mL	8.0053 mL	16.0105 mL	40.0263 mL
	5 mM	0.3202 mL	1.6011 mL	3.2021 mL	8.0053 mL
	10 mM	0.1601 mL	0.8005 mL	1.6011 mL	4.0026 mL
	15 mM	0.1067 mL	0.5337 mL	1.0674 mL	2.6684 mL
	20 mM	0.0801 mL	0.4003 mL	0.8005 mL	2.0013 mL
	25 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6011 mL
	30 mM	0.0534 mL	0.2668 mL	0.5337 mL	1.3342 mL
	40 mM	0.0400 mL	0.2001 mL	0.4003 mL	1.0007 mL
	50 mM	0.0320 mL	0.1601 mL	0.3202 mL	0.8005 mL
	60 mM	0.0267 mL	0.1334 mL	0.2668 mL	0.6671 mL
	80 mM	0.0200 mL	0.1001 mL	0.2001 mL	0.5003 mL
	100 mM	0.0160 mL	0.0801 mL	0.1601 mL	0.4003 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

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营业执照（三证合一）

Verbascoside (Synonyms: 麦角甾苷; Acteoside; Kusaginin; TJC160)

Customer Review

Other Forms of Verbascoside:

MCE 顾客使用本产品发表的 10 篇科研文献