2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. GABA Receptor
  4. (-)-Securinine

(-)-Securinine  (Synonyms: 一叶秋碱)

目录号: HY-N2079 纯度: 99.93%
Data Sheet SDS COA 产品使用指南 技术支持

溶解度

动物溶解方案

(-)-Securinine 是一种植物来源的生物碱，也是一种 GABAA 受体拮抗剂。

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(-)-Securinine Chemical Structure

(-)-Securinine Chemical Structure

CAS No. : 5610-40-2

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥545
In-stock
1 mg ￥228
In-stock
5 mg ￥810
In-stock
10 mg ￥1300
In-stock
25 mg ￥2100
In-stock
50 mg ￥3121
In-stock
100 mg ￥4704
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of (-)-Securinine:

(-)-Securinine 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

(-)-Securinine is plant-derived alkaloid and also a GABAA receptor antagonist.

IC50 & Target

GABAA receptor[1]
体外研究
(In Vitro)

(-)-Securinine is a major plant-derived alkaloid and also a GABAA receptor antagonist. (-)-Securinine is significantly potent on HeLa cells growth inhibition with IC50 values of 7.02±0.52 μg/mL (32.3 μM). (-)-Securinine induces apoptosis in a dose-dependent manner in the tested cells, increases the percentage of ROS positive cells and depolarized cells as well as stimulates the activity of ERK1/2, caspase-9 and -3/7. (-)-Securinine also induces cell cycle arrest in S phase. Real-time PCR analysis shows high expression of tumor necrosis factor receptor superfamily (TNFRSF) genes in the cells stimulated with (-)-Securinine[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

(-)-Securinine 相关抗体:
体内研究
(In Vivo)

In this tumor model, tumor growth is significantly impaired with (-)-Securinine treatment indicating that (-)-Securinine has potential as an Acute Myeloid Leukemia (AML) therapeutic. (-)-Securinine treated mice (n=5 mice, bilateral tumors), exhibit an average of more than 75% smaller tumors than vehicle treated mice at the end of the study period[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

217.26

Formula

C13H15NO2
CAS 号
性状

固体

颜色

Light yellow to yellow

中文名称

一叶秋碱
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 50 mg/mL (230.14 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.6028 mL 23.0139 mL 46.0278 mL
5 mM 0.9206 mL 4.6028 mL 9.2056 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

扫码获得
动物溶解方案

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
纯度 & 产品资料

纯度： 99.93%

Data Sheet (610 KB) SDS (393 KB)

COA (294 KB)HNMR (266 KB)LCMS (86 KB)

产品使用指南 (1538 KB)
参考文献
Kinase Assay
[1]

The cells are seeded in 12-well plates (1×105/well) and treated with (-)-Securinine at concentrations of 1.0 to 50.0 μg/mL. The control cells are exposed to DMSO at a concentration of 0.5% (v/v). After 6 h and 24 h of exposure, the activity of caspase-9 is measured by Caspase-Glo 9 Assay Kit and Glomax Multi+ Detection System, according to the manufacturer’s instruction. The activity of caspase-3/7 is assessed after 24 h of exposure the cells to (-)-Securinine. Then the cells are harvested and prepared using Muse Caspase-3/7 Assay Kit according with the manufacturer’s protocol. The stained cells are analyzed by Muse Cell Analyzer. The experiments are performed at least in three independent repeats[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[1]

The viability of the cells is determined by MTT assay. HeLa cells are seeded in 96-well plates at a density of 5×103 cells/well and treated for 24 h with (-)-Securinine in the concentration range of 1.0 to 20.0 μg/mL. The maximal concentrations of the solvents used in all the MTT experiments are 5.0% (v/v) and 1.0% (v/v) for methanol and DMSO, respectively. The absorption of the obtained formazan solution is measured with a plate reader. The viability results are presented as IC50 mean values of at least three independent experiments[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

6 week old female nude mice are used and injected bilaterally s.c. with 10×106 HL-60 cells. (-)-Securinine treatment is started 10 days after tumor cell injection. Palpable tumors are present for the established tumor model prior to initiating drug treatment. 15 mg/kg of (-)-Securinine or vehicle (30 µL of DMSO and 70 µL of water) are injected i.p. 2 or 3 times a day for 5 days followed by once a day for two days. This injection schedule is repeated for two additional weeks[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

(-)-Securinine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.6028 mL 23.0139 mL 46.0278 mL 115.0695 mL
5 mM 0.9206 mL 4.6028 mL 9.2056 mL 23.0139 mL
10 mM 0.4603 mL 2.3014 mL 4.6028 mL 11.5070 mL
15 mM 0.3069 mL 1.5343 mL 3.0685 mL 7.6713 mL
20 mM 0.2301 mL 1.1507 mL 2.3014 mL 5.7535 mL
25 mM 0.1841 mL 0.9206 mL 1.8411 mL 4.6028 mL
30 mM 0.1534 mL 0.7671 mL 1.5343 mL 3.8357 mL
40 mM 0.1151 mL 0.5753 mL 1.1507 mL 2.8767 mL
50 mM 0.0921 mL 0.4603 mL 0.9206 mL 2.3014 mL
60 mM 0.0767 mL 0.3836 mL 0.7671 mL 1.9178 mL
80 mM 0.0575 mL 0.2877 mL 0.5753 mL 1.4384 mL
100 mM 0.0460 mL 0.2301 mL 0.4603 mL 1.1507 mL

Keywords:

(-)-Securinine5610-40-2一叶秋碱GABA ReceptorGamma-aminobutyric acid Receptorγ-Aminobutyric acid ReceptorInhibitorinhibitorinhibit

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