2. Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation NF-κB
  3. Apoptosis Endogenous Metabolite Reactive Oxygen Species
  4. L-Theanine

L-Theanine  (Synonyms: L-茶氨酸; L-Glutamic Acid γ-ethyl amide; Nγ-Ethyl-L-glutamine)

目录号: HY-15121 纯度: 99.67%
COA 产品使用指南

摩尔计算器

L-Theanine (L-Glutamic Acid γ-ethyl amide) 是一种存在绿茶叶片中的非蛋白氨基酸物质，能阻断大脑中谷氨酸与谷氨酸受体结合，具有神经保护、抗癌和抗氧化活性。L-Theanine 能透过血脑屏障，具有口服活性。

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L-Theanine Chemical Structure

L-Theanine Chemical Structure

CAS No. : 3081-61-6

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥550
In-stock
100 mg ￥500
In-stock
200 mg ￥700
In-stock
500 mg   询价  
1 g   询价  

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Customer Review

Other Forms of L-Theanine:

L-Theanine 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

L-Theanine (L-Glutamic Acid γ-ethyl amide) is a non-protein amino acid contained in green tea leaves, which blocks the binding of L-glutamic acid to glutamate receptors in the brain, and with neuroprotective, anticancer and anti-oxidative activities. L-Theanine can pass through the blood–brain barrier and is orally active[1][2][3].

体外研究
(In Vitro)

L-Theanine (L-Glutamic Acid γ-ethyl amide) inhibits the incorporation of extracellular glutamine into neurons, resulting in the suppression of exocytotic release of glutamate[3].
L-Theanine (500 μM; 72 h) protects against excess Dopamine (HY-B0451)-induced neuronal death in presence of astrocytes and increases glutathione level in astrocytes[3].
L-Theanine (0-5 mM; 72 h) is involved in glutathione synthesis[3].
L-Theanine (0.1-5 mM; 24 h) dose-dependently inhibits the viability of melanoma but not normal epidermal melanocytes[4].
L-Theanine (1-5 mM; 24 h) arrests cell cycle at G0/G1 phase, suppresses cell migration, induces apoptosis in A375 cells[4].
L-Theanine (1-5 mM; 24 h) also affects the proliferation, migration, and apoptosis of B16–F10 melanoma cells[4].
L-Theanine shows protective effect on cadmium-induced apoptosis in PC12 cells by inhibiting the mitochondria-mediated pathway and decreased ROS production[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

L-Theanine 相关抗体:

Cell Proliferation Assay[4]

Cell Line: A375 and PIG1 cells
Concentration: 0.1, 0.5, 1, 2 and 5 mM
Incubation Time: 24 h
Result: Dose-dependently decreased the viability of A375, but not PIG1 cells.

Cell Cycle Analysis[4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Led to a dose dependent accumulation of A375 cells in G0/G1 phase and prevented cells from entering the S phase.

Western Blot Analysis[4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Remarkably reduced the expression of proliferating cell nuclear antigen (PCNA), decreased protein levels of cyclinD1, cyclinE1, and cyclin-dependent protein kinase (CDK2 and CDK4). Potentiated the expression of cyclin-dependent kinase inhibitor 1A (CDKN1A, p21).
Dose-dependently increased the levels of apoptosis-promoting proteins including BAX and cleaved-caspase3 and decreased the level of antiapoptotic protein BCL-2.
Concentration dependently reduced the protein levels of ICAM-1, VCAM-1, MMP9, and MMP2.
Dose-dependently increased the p53 expression.

Cell Migration Assay [4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Apparently suppressed the migration.

Apoptosis Analysis[4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Showed pro-apoptotic effect.

RT-PCR[4]

Cell Line: A375
Concentration: 1, 2 and 5 mM
Incubation Time: 24 h
Result: Markedly elevated the mRNA levels of Bmal1, Clock, Rora, and Rev-erbβ.
体内研究
(In Vivo)

L-Theanine (4.0 mg/kg; p.o.; daily for 14 days) upregulates glutathione contents in striata of normal mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Healthy male ICR mice weighing 32–34 g[3]
Dosage: 4.0 mg/kg
Administration: Oral administration, daily for 14 days
Result: Significantly increased glutathione content in the striatum, but not in the midbrain.
分子量

174.20

Formula

C7H14N2O3
CAS 号
性状

固体

颜色

White to off-white

中文名称

L-茶氨酸；茶氨酸
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验： 

H2O 中的溶解度 : 150 mg/mL (861.08 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.7405 mL 28.7026 mL 57.4053 mL
5 mM 1.1481 mL 5.7405 mL 11.4811 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： PBS

    Solubility: 100 mg/mL (574.05 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.67%

COA (280 KB) RP-HPLC (276 KB) LCMS (88 KB)

产品使用指南 (1538 KB)
参考文献

L-Theanine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 5.7405 mL 28.7026 mL 57.4053 mL 143.5132 mL
5 mM 1.1481 mL 5.7405 mL 11.4811 mL 28.7026 mL
10 mM 0.5741 mL 2.8703 mL 5.7405 mL 14.3513 mL
15 mM 0.3827 mL 1.9135 mL 3.8270 mL 9.5675 mL
20 mM 0.2870 mL 1.4351 mL 2.8703 mL 7.1757 mL
25 mM 0.2296 mL 1.1481 mL 2.2962 mL 5.7405 mL
30 mM 0.1914 mL 0.9568 mL 1.9135 mL 4.7838 mL
40 mM 0.1435 mL 0.7176 mL 1.4351 mL 3.5878 mL
50 mM 0.1148 mL 0.5741 mL 1.1481 mL 2.8703 mL
60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3919 mL
80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7939 mL
100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4351 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Theanine3081-61-6L-Glutamic Acid γ-ethyl amide Nγ-Ethyl-L-glutamineL-茶氨酸茶氨酸ApoptosisEndogenous MetaboliteReactive Oxygen Speciesneuroprotectiveanticanceranti-oxidativeanti-stressglutathioneGSHInhibitorinhibitorinhibit

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