1. Autophagy Cell Cycle/DNA Damage Anti-infection
  2. Autophagy CRISPR/Cas9 Mitophagy HSV Antibiotic Bacterial
  3. Brefeldin A

Brefeldin A  (Synonyms: 布雷非德菌素 A; BFA; Cyanein; Decumbin)

目录号: HY-16592 纯度: 99.82%
COA 产品使用指南

Brefeldin A (BFA) 是一种内酯抗生素,是蛋白质运输的特定抑制剂。Brefeldin A 阻断分泌蛋白和膜蛋白从内质网向高尔基体的转运。Brefeldin A 也是一种自噬 (autophagy) 和 mitophagy 抑制剂。Brefeldin A 还是一种 CRISPR/Cas9 激动剂,可抑制 HSV-1病毒,并具有抗癌活性。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Brefeldin A Chemical Structure

Brefeldin A Chemical Structure

CAS No. : 20350-15-6

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10 mM * 1 mL in DMSO ¥567
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5 mg ¥515
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10 mg ¥850
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50 mg ¥2980
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100 mg ¥4600
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Customer Review

MCE 顾客使用本产品发表的 80 篇科研文献

Proliferation Assay
WB
IP

    Brefeldin A purchased from MCE. Usage Cited in: Cell Rep. 2023 Mar 26;42(4):112306.  [Abstract]

    Brefeldin A (BFA) slightly reduces the ubiquitination of STING in HEK293T cells (transfection with Myc-STING, FLAG-TRIM10, and HA-Ub for 24 h).

    Brefeldin A purchased from MCE. Usage Cited in: J Cell Mol Med. 2019 Apr;23(4):2399-2409.  [Abstract]

    MHCC-97H cells are treated with BFA (2.5 μg/mL) for 0, 0.5, 1, 2, 6, and 12 h. The expression of GP73 and intracellular MMP-2 is measured using immunoblotting.

    Brefeldin A purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.  [Abstract]

    The interaction of Beclin 1 with Bcl-2 or ATG14L after BFA treatment is determined by coimmunoprecipitation assay.

    Brefeldin A purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.  [Abstract]

    CRC cells are treated with vehicle, BFA (100 nM), 3-MA (10 mM), or in combination for 24 h. Immunoblot analysis is used to detect LC3 turnover.

    Brefeldin A purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.  [Abstract]

    Apoptotic effects induced by BFA are examined in CRC cells.

    Brefeldin A purchased from MCE. Usage Cited in: J Radiat Res. 2017 Jul 1;58(4):474-486.  [Abstract]

    Images showing the morphology of axons after 2.0 mT of 50 Hz MF exposure for 3 days, 1 h per day. Histograms showing the average axon length per neuron, the average axon branch length per neuron and the average number of branches per axon in the sham and exposure groups. Neurons were treated with 1.0 µg/mL brefeldin A at DIV2 as a positive control.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Brefeldin A (BFA) is a lactone antibiotic and a specific inhibitor of protein trafficking. Brefeldin A blocks the transport of secreted and membrane proteins from endoplasmic reticulum to Golgi apparatus[1][2]. Brefeldin A is also an autophagy and mitophagy inhibitor[3]. Brefeldin A is a CRISPR/Cas9 activator[5]. Brefeldin A inhibits HSV-1 and has anti-cancer activity[5].

    IC50 & Target[5][6]

    CRISPR/Cas9

     

    HSV-1

     

    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    Bel-7402 IC50
    0.024 μM
    Compound: BFA; 1
    Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay
    Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay
    [PMID: 29524728]
    Bel-7402 IC50
    0.07 μM
    Compound: BFA
    Antiproliferative activity against human Bel-7402 cells by MTT assay
    Antiproliferative activity against human Bel-7402 cells by MTT assay
    [PMID: 36089748]
    Bel-7402 IC50
    0.17 μM
    Compound: 1; BFA
    Antiproliferative activity against human Bel-7402 cells measured after 72 hrs by MTT assay
    Antiproliferative activity against human Bel-7402 cells measured after 72 hrs by MTT assay
    [PMID: 35849940]
    Bel7402/5-FU IC50
    0.82 μM
    Compound: BFA; 1
    Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay
    Antiproliferative activity against human Bel7402/5-FU cells after 72 hrs by MTT assay
    [PMID: 29524728]
    ECa-109 cell line IC50
    0.14 μM
    Compound: 1; BFA
    Antiproliferative activity against human Eca-109 cells measured after 72 hrs by MTT assay
    Antiproliferative activity against human Eca-109 cells measured after 72 hrs by MTT assay
    [PMID: 35849940]
    HeLa IC50
    0.07 μM
    Compound: 1; BFA
    Antiproliferative activity against human HeLa cells measured after 72 hrs by MTT assay
    Antiproliferative activity against human HeLa cells measured after 72 hrs by MTT assay
    [PMID: 35849940]
    HeLa EC50
    5 μM
    Compound: Brefeldin A
    Inhibition of Escherichia coli Stx1 in human HeLa cells assessed as stimulation of protein synthesis by measuring increase in [14C]-leucine incorporation incubated with cells for 1 hr prior to Stx1 addition and further incubated for 1 to 24 hrs and subseq
    Inhibition of Escherichia coli Stx1 in human HeLa cells assessed as stimulation of protein synthesis by measuring increase in [14C]-leucine incorporation incubated with cells for 1 hr prior to Stx1 addition and further incubated for 1 to 24 hrs and subseq
    [PMID: 32648758]
    HepG2 IC50
    0.08 μM
    Compound: BFA
    Antiproliferative activity against human HepG2 cells by MTT assay
    Antiproliferative activity against human HepG2 cells by MTT assay
    [PMID: 36089748]
    HepG2 IC50
    0.18 μM
    Compound: 1; BFA
    Antiproliferative activity against human HepG2 cells measured after 72 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells measured after 72 hrs by MTT assay
    [PMID: 35849940]
    HepG2 IC50
    0.35 μM
    Compound: 1; BFA
    Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay
    [PMID: 28494251]
    HL-60 IC50
    0.025 μM
    Compound: BFA; 1
    Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
    Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
    [PMID: 29524728]
    HT-29 IC50
    0.16 μM
    Compound: 1; BFA
    Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay
    Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay
    [PMID: 28494251]
    L02 IC50
    < 0.0004 μM
    Compound: 1; BFA
    Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay
    Cytotoxicity against human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay
    [PMID: 28494251]
    L02 IC50
    < 0.001 μM
    Compound: BFA; 1
    Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay
    Antiproliferative activity against human LO2 cells after 72 hrs by MTT assay
    [PMID: 29524728]
    L02 IC50
    0.1 μM
    Compound: 1; BFA
    Cytotoxicity against human L02 cells measured after 72 hrs by MTT assay
    Cytotoxicity against human L02 cells measured after 72 hrs by MTT assay
    [PMID: 35849940]
    PANC-1 IC50
    0.08 μM
    Compound: 1; BFA
    Antiproliferative activity against human PANC-1 cells measured after 72 hrs by MTT assay
    Antiproliferative activity against human PANC-1 cells measured after 72 hrs by MTT assay
    [PMID: 35849940]
    PC-3 IC50
    0.068 μM
    Compound: BFA; 1
    Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay
    Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay
    [PMID: 29524728]
    PC-3 IC50
    0.068 μM
    Compound: 1; BFA
    Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay
    Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay
    [PMID: 28494251]
    体外研究
    (In Vitro)

    Brefeldin A (BFA) 处理 15 小时或 40 小时,会导致内质网 (ER) 显著肿胀,并将其定位转移到正常大鼠肾 (NRK) 细胞的外围。延长 Brefeldin A 处理会导致 MT 和肌动蛋白细胞骨架显著破坏[1]。BARS 的 ADP-核糖基化是由 Brefeldin A 和 ADPR 之间形成结合物介导的。当与来自 BFA 处理的 CD38+ HeLa 细胞[3]的培养基一起孵育时,BARS 显示 BAC 结合。Brefeldin A 在 MDA-MB-231 乳腺癌细胞中诱导不依赖锚定的细胞死亡,抑制 3D 和 2D 培养物中 MDA-MB-231 集落的形成,并抑制 MDA-MB 的迁移和 MMP 9 (基质金属肽酶 9) 活性-231[2]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    280.36

    Formula

    C16H24O4

    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    布雷非德菌素 A

    结构分类
    初始来源

    Penicillium

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    细胞实验: 

    DMSO 中的溶解度 : 100 mg/mL (356.68 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    Ethanol 中的溶解度 : 11.11 mg/mL (39.63 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.5668 mL 17.8342 mL 35.6684 mL
    5 mM 0.7134 mL 3.5668 mL 7.1337 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.92 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.92 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。
    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.82%

    参考文献
    Cell Assay
    [1]

    Cells are grown on glass coverslips, fixed in 3 % paraformaldehyde in PBS (10 min at room temperature) and then washed in PBS. Cells are permeabilized with 0.01 % Triton X-lOO in PBS at room temperature for 7 min. The coverslips are washed (3 times in PBS/0.2 % Tween) incubated in PBS/O.4 % fish skin gelatin/0.2 % Tween (5 min) and in PBS/2.5 % goat serum/0.2 % Tween (5 min.). After blocking, the cells are incubated with primary antibodies for 45 min at 37°C, and then washed with PBS/0.2 % Tween (5 times, 5 min each). The secondary antibodies are added for 30 min at 37°C and then cells are washed as above. Coverslips are mounted on slides in 9: 1 glycerol/PBS with 0.1 % o-phenylenediamine.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 3.5668 mL 17.8342 mL 35.6684 mL 89.1711 mL
    5 mM 0.7134 mL 3.5668 mL 7.1337 mL 17.8342 mL
    10 mM 0.3567 mL 1.7834 mL 3.5668 mL 8.9171 mL
    15 mM 0.2378 mL 1.1889 mL 2.3779 mL 5.9447 mL
    20 mM 0.1783 mL 0.8917 mL 1.7834 mL 4.4586 mL
    25 mM 0.1427 mL 0.7134 mL 1.4267 mL 3.5668 mL
    30 mM 0.1189 mL 0.5945 mL 1.1889 mL 2.9724 mL
    DMSO 40 mM 0.0892 mL 0.4459 mL 0.8917 mL 2.2293 mL
    50 mM 0.0713 mL 0.3567 mL 0.7134 mL 1.7834 mL
    60 mM 0.0594 mL 0.2972 mL 0.5945 mL 1.4862 mL
    80 mM 0.0446 mL 0.2229 mL 0.4459 mL 1.1146 mL
    100 mM 0.0357 mL 0.1783 mL 0.3567 mL 0.8917 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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