Dacarbazine (Synonyms: 达卡巴嗪; Imidazole Carboxamide)

目录号: HY-B0078 纯度: 99.96%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Dacarbazine 是一种非特异性抗肿瘤 (antineoplastic) 烷基化剂。Dacarbazine 抑制 T 和 B 淋巴细胞反应，IC₅₀ 分别为 50 和 10 μg/mL。Dacarbazine 可用于转移性恶性黑色素瘤的研究

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Dacarbazine Chemical Structure

CAS No. : 4342-03-4

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥660	In-stock
50 mg	￥280	In-stock
100 mg	￥460	In-stock
200 mg	￥600	In-stock
1 g	￥1000	In-stock
5 g		询价
10 g		询价

or 大包装询价

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Customer Review

Other Forms of Dacarbazine:

Bismuth potassium citrate In-stock
Lithium citrate tetrahydrate In-stock
Citric acid triammonium In-stock
Sodium citrate dihydrate In-stock
Sodium citrate dihydrate, meets USP testing specifications In-stock
Ammonium iron(III) citrate In-stock
Citric acid trisodium In-stock
Citric acid In-stock
Citric acid monohydrate In-stock
Citric acid tripotassium hydrate In-stock
Dacarbazine-d₆ 询价
Dacarbazine citrate 询价
Dacarbazine hydrochloride 询价
Ammonium citrate dibasic, 99% 询价
Sodium citrate dihydrate, United States Pharmacopeia (USP) Reference Standard 询价
Sodium citrate dihydrate, for molecular biology 询价
Lithium citrate 询价
Hydroxycitric acid 询价
Bismuth(Ⅲ) citrate, 99.99% trace metals basis 询价
Dacarbazine (Standard) 询价

MCE 顾客使用本产品发表的 26 篇科研文献

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生物活性
纯度 & 产品资料
参考文献

生物活性

Dacarbazine is a nonspecific antineoplastic (antineoplastic) alkylating agent. Dacarbazine inhibits T and B lymphocyte responses with IC₅₀ of 50 and 10 μg/mL, respectively. Dacarbazine can be used in the study of metastatic malignant melanoma^[1]^[2]^[3]^[4]^[5].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-375	IC₅₀	> 100 μM Compound: DTIC	Antiproliferative activity against human A375 cells after 48 hrs by SRB assay Antiproliferative activity against human A375 cells after 48 hrs by SRB assay	[PMID: 20056548]
A-375	IC₅₀	> 100 μM Compound: DTIC, dacarbazine	Antiproliferative activity against human A375 cells after 48 hrs by SRB assay Antiproliferative activity against human A375 cells after 48 hrs by SRB assay	[PMID: 18477505]
A-375	IC₅₀	> 500 μM Compound: dacarbazine	Cytotoxicity against human A375 cells after 48 hrs by MTT assay Cytotoxicity against human A375 cells after 48 hrs by MTT assay	[PMID: 22560315]
A549	IC₅₀	35 μg/mL Compound: Dacarbazine	Cytotoxicity against Homo sapiens (human) A549 cells assessed as cell viability by MTT assay Cytotoxicity against Homo sapiens (human) A549 cells assessed as cell viability by MTT assay	10.1007/s00044-007-9031-x
B16-F1	IC₅₀	> 100 μM Compound: DTIC	Antiproliferative activity against mouse B16-F1 cells after 48 hrs by SRB assay Antiproliferative activity against mouse B16-F1 cells after 48 hrs by SRB assay	[PMID: 20056548]
B16-F1	IC₅₀	> 100 μM Compound: DTIC, dacarbazine	Antiproliferative activity against mouse B16-F1 cells after 48 hrs by SRB assay Antiproliferative activity against mouse B16-F1 cells after 48 hrs by SRB assay	[PMID: 18477505]
Fibroblast	IC₅₀	> 100 μM Compound: DTIC	Cytotoxicity against human fibroblasts after 48 hrs by SRB assay Cytotoxicity against human fibroblasts after 48 hrs by SRB assay	[PMID: 20056548]
HeLa	GI₅₀	> 50 μM Compound: DTIC	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 24 to 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 24 to 48 hrs by MTT assay	[PMID: 24986661]
OVCAR-3	IC₅₀	17.67 μM Compound: Dacarbazine	Cytotoxicity against human OVCAR3 cells after 6 days by MTT assay Cytotoxicity against human OVCAR3 cells after 6 days by MTT assay	[PMID: 21131205]
SK-MEL-2	IC₅₀	120 μM Compound: Dacarbazine	Cytotoxicity against human SK-MEL-2 cells after 6 days by MTT assay Cytotoxicity against human SK-MEL-2 cells after 6 days by MTT assay	[PMID: 21131205]
UACC-62	IC₅₀	11.83 μM Compound: Dacarbazine	Cytotoxicity against human UACC62 cells after 6 days by MTT assay Cytotoxicity against human UACC62 cells after 6 days by MTT assay	[PMID: 21131205]
WM164	IC₅₀	> 100 μM Compound: DTIC	Antiproliferative activity against human WM164 cells after 48 hrs by SRB assay Antiproliferative activity against human WM164 cells after 48 hrs by SRB assay	[PMID: 20056548]
WM164	IC₅₀	> 100 μM Compound: DTIC	Antiproliferative activity against human WM164 cells after 48 hrs by SRB assay Antiproliferative activity against human WM164 cells after 48 hrs by SRB assay	[PMID: 17561392]

体外研究
(In Vitro)

Dacarbazine (6.25-500 μg/mL 48 h) 联合热疗可诱导 A375 和 MNT-1 黑色素瘤细胞的细胞毒性^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[5]

Cell Line:	A375, MNT-1
Concentration:	6.25, 12.5, 25, 50, 100, 200, 400, 500 μg/mL
Incubation Time:	24, 48, 72 h
Result:	Inhibited cell viability in a concentration-dependent manner.

Cell Cycle Analysis^[5]

Cell Line:	A375, MNT-1
Concentration:	5.5, 115 μg/mL
Incubation Time:	48 h
Result:	Decreased (9.3%) the percentage of A375 cells at the S phase and increased the number of cells at G2/M. Decreased MNT-1 cells at G0/G1, increasing in cells at both S and G2/M phases.

Clinical Trial

分子量

182.18

Formula

C₆H₁₀N₆O

CAS 号

4342-03-4

性状

固体

颜色

White to yellow

中文名称

达卡巴嗪；氨烯咪胺；甲嗪咪唑胺；三嗪咪唑胺

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

细胞实验：

0.1 M HCL 中的溶解度 : 20 mg/mL (109.78 mM; 超声助溶 (<60°C))

DMSO 中的溶解度 : 5 mg/mL (27.45 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.4891 mL	27.4454 mL	54.8908 mL
5 mM	1.0978 mL	5.4891 mL	10.9782 mL
10 mM	0.5489 mL	2.7445 mL	5.4891 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 2 mg/mL (10.98 mM); 澄清溶液; 超声助溶 (<60°C)
方案二

请依序添加每种溶剂： 50% PEG300 50% Saline
Solubility: 5 mg/mL (27.45 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

纯度 & 产品资料

纯度: 99.96%

选择批次：

Data Sheet (623 KB) SDS (419 KB)

COA (198 KB) HNMR (252 KB) RP-HPLC (257 KB) MS (206 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Serrone, L., et al., Dacarbazine-based chemotherapy for metastatic melanoma: thirty-year experience overview. J Exp Clin Cancer Res, 2000. 19(1): p. 21-34. [Content Brief]

[2]. Al-Badr AA, et al. Dacarbazine. Profiles Drug Subst Excip Relat Methodol. 2016;41:323-77. [Content Brief]

[3]. Rojo JM, et al. Inhibition of T and B lymphoblastic response by mithramycin, dacarbazine, prospidium chloride and peptichemio. Chemotherapy. 1983;29(5):345-51. [Content Brief]

[4]. Erdmann S, et al. Induced cross-resistance of BRAFV600E melanoma cells to standard chemotherapeutic dacarbazine after chronic PLX4032 treatment. Sci Rep. 2019 Jan 10;9(1):30. [Content Brief]

[5]. Salvador D, et al. Combined Therapy with Dacarbazine and Hyperthermia Induces Cytotoxicity in A375 and MNT-1 Melanoma Cells. Int J Mol Sci. 2022 Mar 25;23(7):3586. [Content Brief]

Dacarbazine 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO / 0.1 M HCL	1 mM	5.4891 mL	27.4454 mL	54.8908 mL	137.2269 mL
	5 mM	1.0978 mL	5.4891 mL	10.9782 mL	27.4454 mL
	10 mM	0.5489 mL	2.7445 mL	5.4891 mL	13.7227 mL
	15 mM	0.3659 mL	1.8297 mL	3.6594 mL	9.1485 mL
	20 mM	0.2745 mL	1.3723 mL	2.7445 mL	6.8613 mL
	25 mM	0.2196 mL	1.0978 mL	2.1956 mL	5.4891 mL
0.1 M HCL	30 mM	0.1830 mL	0.9148 mL	1.8297 mL	4.5742 mL
	40 mM	0.1372 mL	0.6861 mL	1.3723 mL	3.4307 mL
	50 mM	0.1098 mL	0.5489 mL	1.0978 mL	2.7445 mL
	60 mM	0.0915 mL	0.4574 mL	0.9148 mL	2.2871 mL
	80 mM	0.0686 mL	0.3431 mL	0.6861 mL	1.7153 mL
	100 mM	0.0549 mL	0.2745 mL	0.5489 mL	1.3723 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dacarbazine4342-03-4Imidazole Carboxamide达卡巴嗪氨烯咪胺甲嗪咪唑胺三嗪咪唑胺Nucleoside Antimetabolite/AnalogApoptosisMelanomaA375MNT-1AntitumorInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Dacarbazine (Synonyms: 达卡巴嗪; Imidazole Carboxamide)

Customer Review

Other Forms of Dacarbazine:

MCE 顾客使用本产品发表的 26 篇科研文献