2. Anti-infection Metabolic Enzyme/Protease Apoptosis Membrane Transporter/Ion Channel Neuronal Signaling
  3. Bacterial Antibiotic HIF/HIF Prolyl-Hydroxylase Apoptosis MDM-2/p53 Potassium Channel Calcium Channel
  4. Minocycline hydrochloride

Minocycline hydrochloride  (Synonyms: 盐酸米诺环素)

目录号: HY-17412 纯度: 99.79%
COA 产品使用指南

摩尔计算器

Minocycline hydrochloride 是一种口服有效、能透过血脑屏障的半合成四环素类抗生素。Minocycline hydrochloride 是一种缺氧诱导因子 (HIF-1α) 抑制剂。Minocycline hydrochloride 具有抗癌(anti-cancer)，抗炎(anti-inflammatory) 和谷氨酸 (glutamate) 拮抗作用。Minocycline hydrochloride 降低谷氨酸神经传递，显示神经保护特性和抗抑郁作用。Minocycline hydrochloride 通过与细菌核糖体30S亚基结合，抑制细菌蛋白的合成，从而产生抑菌 (bacteriostatic) 作用。

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Minocycline hydrochloride Chemical Structure

Minocycline hydrochloride Chemical Structure

CAS No. : 13614-98-7

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥500
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50 mg ￥450
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100 mg ￥650
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Customer Review

Other Forms of Minocycline hydrochloride:

Minocycline hydrochloride 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 36 篇科研文献

Proliferation Assay
IF

    Minocycline hydrochloride purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Aug 30;15(1):245.  [Abstract]

    Immunostaining of the trigeminal nucleus caudalis (TNC) for Iba1 in the NTG group and the NTG+Minocycline (Mino) group on day 9.

    Minocycline hydrochloride purchased from MCE. Usage Cited in: Neurochem Res. 2017 Oct;42(10):2698-2711.  [Abstract]

    a Statistical analysis showing the prevention effect of minocycline pretreatment (40 mg/kg/day) on CUS-, CRS- or CSDS-induced decreases in hippocampal microglial numbers. b, c Statistical analysis showing the prevention effect of minocycline pretreatment (40 mg/kg/day) on CUS-, CRS- or CSDS-induced increases in the immobile time in the TST (b) and FST (c).

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    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Minocycline hydrochloride is an orally active, potent and BBB-penetrated semi-synthetic tetracycline antibiotic. Minocycline hydrochloride is a hypoxia-inducible factor (HIF)-1α inhibitor. Minocycline hydrochloride shows anti-cancer, anti-inflammatory, and glutamate antagonist effects. Minocycline hydrochloride reduces glutamate neurotransmission and shows neuroprotective properties and antidepressant effects. Minocycline hydrochloride inhibits bacterial protein synthesis through binding with the 30S subunit of the bacterial ribosome, resulting in a bacteriostatic effect[1][2][3][4][5][6][7].

    IC50 & Target

    L-type calcium channel

     

    体外研究
    (In Vitro)

    Minocycline hydrochloride (0-100 μM，24-72 小时) 抑制卵巢癌细胞系 (OVCAR-3、SKOV-3 和 A2780) 的增殖和克隆形成活性[3]。
    Minocycline hydrochloride (0-100 μM，24-48 h) 通过抑制细胞周期蛋白和抑制 DNA 掺入来抑制细胞周期[3]。
    Minocycline hydrochloride (0-100 μM，72 h)) 在卵巢癌细胞系中诱导细胞凋亡[3]。
    Minocycline hydrochloride 显示出直接的神经元保护作用，这种保护模式可能与线粒体完整性和细胞色素 c 的保护有关，随后抑制 caspase 依赖性和非 caspase 依赖性细胞死亡[2]。
    Minocycline hydrochloride 导致缺氧诱导因子 (HIF)-1α 的抑制，并伴有 up-p53 蛋白水平的调节和 AKT/mTOR/p70S6K/4E-BP1 通路的失活[6]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Minocycline hydrochloride 相关抗体:

    Cell Proliferation Assay[3]

    Cell Line: Human ovarian cancer cell lines (OVCAR-3, SKOV-3 and A2780) and primary cells (HEK-293, HMEC, HUVEC, ATCC)
    Concentration: 0, 1, 10, 50 and 100 μM
    Incubation Time: 24, 48 or 72 h
    Result: Inhibited proliferation of OVCAR-3, SKOV-3 and A2780 cells in a concentration-dependent manner, with IC50 values of 62.0, 56.1 and 59.5 μM, respectively. Had no effect on the viability of HEK-293 or HUVEC.

    Western Blot Analysis[3]

    Cell Line: OVCAR-3, SKOV-3 and A2780 cells
    Concentration: 0, 10, 50 and 100 μM
    Incubation Time: 72 h
    Result: Expressed lower levels of cyclins A, B and E. Increased caspase-3 levels by more than 3.0 fold in the 100 μM. Minocycline-activated caspase-3 in turn led to cleavage of PARP-1. Increased the degradation product p89 of PARP-1 by caspase-3.

    Cell Cycle Analysis[3]

    Cell Line: OVCAR-3, SKOV-3 and A2780 cells
    Concentration: 0, 10, 50 and 100 μM
    Incubation Time: 24 or 48 h
    Result: Arrested cells in the G0-G1 phase in a concentration and time-dependent manner. Declined percentage of cells in the S and G2-M phases in excess of 80% each at 100 μM.
    体内研究
    (In Vivo)

    Minocycline hydrochloride (0-30 mg/kg，口服，每日一次，持续 4 周) 抑制雌性裸鼠 OVCAR-3 肿瘤的生长[3]。
    Minocycline hydrochloride (IP) 是一种有效的药物当腹腔内给予高剂量时，脑缺血动物模型中的神经保护剂[1]。
    Minocycline hydrochloride (0-40 mg/kg，IP，一次) 显著减弱 METH 诱导的过度运动和小鼠行为敏化的发展[2]。
    Minocycline hydrochloride (3 和 10 mg/kg，IV，一次) 可有效减少临时大脑中动脉闭塞模型中的梗塞面积 (TMCAO)[1]。
    Minocycline hydrochloride (3-10 mg/kg，IV，一次) 导致血清水平 (3 mg/kg) 与人类在标准 200 mg 剂量[1]。
    Minocycline hydrochloride 可减轻大鼠缺血引起的室性心律失常。这种作用可能与 PI3K/Akt 信号通路、线粒体 KATP 通道和 L 型 Ca2+ 通道的激活有关[7]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female nude mice (6 weeks old, 9 per group, OVCAR-3 cells were injected s.c. into the left flank of each mouse)[3]
    Dosage: 10 or 30 mg/kg
    Administration: Administered orally in the drinking water, initiated on day 8 of cell inoculation, daily for 4 weeks
    Result: Suppressed OVCAR-3 tumor growth in these female nude mice, and reduced microvessel density.
    Animal Model: Male Balb/cAnNCrICrIj mice (8 weeks old, 23-30 g, methamphetamine (METH, 3 mg/kg) was injected subcutaneously (s.c.) in a volume of 10 ml/kg)[2]
    Dosage: 0, 10, 20, or 40 mg/kg
    Administration: IP, once, 30 min before the administration of METH
    Result: Significantly attenuated METH-induced hyperlocomotion and the development of behavioral sensitization in mice at 40 mg/kg. Did not exert any effect on the induction of METH-induced hyperthermia in mice. Significantly attenuated the reduction of DA and DOPAC in the striatum. Significantly attenuated the reduction of DAT-immunoreactivity in the mouse striatum. Significantly attenuated the increase in MAC1-immunoreactivity in the striatum after the administration of METH.
    Animal Model: Male Sprague-Dawley rats (270-330 g, TMCAO model)[1]
    Dosage: 3 mg/kg and 10 mg/kg
    Administration: IV, once, 4, 5, or 6 hours post TMCAO
    Result: Reduced infarct size by 42% while 10 mg/kg reduced infarct size by 56% at doses of 3 mg/kg; significantly reduced infarct size at 5 hours by 40% at doses of 10 mg/kg and the 3 mg/kg dose significantly reduced infarct size by 34%. With a 6 hour time window there was a non-significant trend in infarct reduction.
    Animal Model: Male Sprague-Dawley rats (270-330 g)[1]
    Dosage: 3, 10, or 20 mg/kg
    Administration: IV, once
    Result: Peak concentrations of serum levels of minocycline averaged 3.6, 13.0 and 28.8 mg/L with 3, 10 and 20 mg/kg doses respectively. The serum levels of minocycline at a 3 mg/kg dose (3.6 mg/L) were similar to that reported in humans after a standard 200 mg dose. Did not significantly affect hemodynamic and physiological variables.
    Clinical Trial
    分子量

    493.94

    Formula

    C23H28ClN3O7
    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    中文名称

    盐酸米诺环素；二甲胺四环素；二甲胺四环素盐酸盐
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 19.23 mg/mL (38.93 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 9.09 mg/mL (18.40 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.0245 mL 10.1227 mL 20.2454 mL
    5 mM 0.4049 mL 2.0245 mL 4.0491 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 7.69 mg/mL (15.57 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.79%

    COA (199 KB) HNMR (271 KB) LCMS (267 KB)

    产品使用指南 (1538 KB)
    参考文献

    Minocycline hydrochloride 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.0245 mL 10.1227 mL 20.2454 mL 50.6134 mL
    5 mM 0.4049 mL 2.0245 mL 4.0491 mL 10.1227 mL
    10 mM 0.2025 mL 1.0123 mL 2.0245 mL 5.0613 mL
    15 mM 0.1350 mL 0.6748 mL 1.3497 mL 3.3742 mL
    DMSO 20 mM 0.1012 mL 0.5061 mL 1.0123 mL 2.5307 mL
    25 mM 0.0810 mL 0.4049 mL 0.8098 mL 2.0245 mL
    30 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6871 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Minocycline13614-98-7盐酸米诺环素二甲胺四环素二甲胺四环素盐酸盐BacterialAntibioticHIF/HIF Prolyl-HydroxylaseApoptosisMDM-2/p53Potassium ChannelCalcium ChannelHypoxia-inducible factorsHIFsHIF-PHKcsACa2+ channelsCa channelscerebral ischemianeuroprotectivestrokeVentricular arrhythmiaMyocardial ischemiaPI3K/AktMitochondrial ATP-sensitive potassiumL-type Ca2+ channelacinetobacterinfectionovarian cancerInhibitorinhibitorinhibit

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