Niclosamide (Synonyms: 氯硝柳胺; BAY2353)

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Niclosamide (BAY2353) 是一种具有口服活性的用于寄生虫感染研究的抗蠕虫化合物。Niclosamide 是 STAT3 抑制剂，在 HeLa 细胞中的 IC₅₀ 为 0.25 μM。Niclosamide 具有抗癌的生物活性，并能抑制 Vero E6 细胞的 DNA 复制。

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Niclosamide Chemical Structure

CAS No. : 50-65-7

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥550	In-stock
500 mg	￥400	In-stock
5 g	￥500	In-stock
10 g	￥800	In-stock
50 g		询价

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Customer Review

Other Forms of Niclosamide:

MCE 顾客使用本产品发表的 27 篇科研文献

Cell Viability Assay

Proliferation Assay

: Niclosamide purchased from MCE. Usage Cited in: Biomed Pharmacother. 2023 May.; Niclosamide (NIC) significantly suppresses the colony formation ability, and reduces the numbers, as well as diameters, of colonies formed in AN3CA, Hec1B, and Ishikawa cells.

: Niclosamide purchased from MCE. Usage Cited in: Biomed Pharmacother. 2023 May.; Niclosamide (NIC; 1 μM) reduces number of cells that invaded across the trans-well chamber in Hec1B and Ishikawa endometrial cancer cell lines, suggesting NIC-mediated inhibition of invasive property of endometrial cancer cells.

: Niclosamide purchased from MCE. Usage Cited in: Biomed Pharmacother. 2023 May.; Niclosamide (NIC; 0-8 μM; 24, 48, 72 h) significantly inhibits the metabolic viability of AN3CA, Hec1B, and Ishikawa cells both in a dose-dependent and a time-dependent manner, with the half maximal inhibitory concentration of < 1 μM at 72 h.

: Niclosamide purchased from MCE. Usage Cited in: Biomed Pharmacother. 2023 May.; Niclosamide (NIC; 5 μM; 24 h) significantly reduces cyclin D1 and vimentin protein expression in human endometrial cancer cells.

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生物活性
纯度 & 产品资料
参考文献

生物活性

Niclosamide (BAY2353) is an orally active antihelminthic agent used in parasitic infection research^[1]. Niclosamide is a STAT3 inhibitor with an IC₅₀ of 0.25 μM in HeLa cells^[4]. Niclosamide has biological activities against cancer, inhibits DNA replication in Vero E6 cells^[2]^[3]^[5].

IC₅₀ & Target

STAT3

0.25 μM (IC₅₀, in HeLa cells)

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-431	IC₅₀	8.8 μM Compound: 5	Antiproliferative activity against human A431 cells after 72 hrs by MTT assay Antiproliferative activity against human A431 cells after 72 hrs by MTT assay	[PMID: 24900231]
A549	IC₅₀	0.4 μM Compound: Niclosamide	Antiproliferative activity against human A549 cells after 120 hrs by MTT assay Antiproliferative activity against human A549 cells after 120 hrs by MTT assay	[PMID: 16680159]
A549	CC₅₀	22.9 μM Compound: 6	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay	[PMID: 32045239]
A549	CC₅₀	22.9 μM Compound: 1	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by alamarBlue assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by alamarBlue assay	[PMID: 33112138]
A549	IC₅₀	3 μM Compound: 5	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 24900231]
ASPC1	IC₅₀	1.47 μM Compound: 1	Cytotoxicity against human AsPC1 cells after 72 hrs by MTS assay Cytotoxicity against human AsPC1 cells after 72 hrs by MTS assay	[PMID: 23459613]
ASPC1	IC₅₀	1.47 μM Compound: Niclosamide	CYtotoxicity against human AsPC1 cells by MTS assay CYtotoxicity against human AsPC1 cells by MTS assay	[PMID: 23416191]
BHK-21	CC₅₀	> 20 μM Compound: 104	Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay	[PMID: 28689975]
Bone marrow cell	IC₅₀	> 10 μM Compound: 89	Cytotoxicity against human bone marrow cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human bone marrow cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31253529]
Cancer cell lines	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human breast cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay Cytotoxicity against human breast cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay	[PMID: 26272032]
Cancer cell lines	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human colon cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay Cytotoxicity against human colon cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay	[PMID: 26272032]
Cancer cell lines	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human lung cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay Cytotoxicity against human lung cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay	[PMID: 26272032]
Cancer cell lines	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human prostate cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay Cytotoxicity against human prostate cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay	[PMID: 26272032]
Cancer cell lines	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human ovarian cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay Cytotoxicity against human ovarian cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay	[PMID: 26272032]
Cancer cell lines	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human blood cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay Cytotoxicity against human blood cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay	[PMID: 26272032]
Cancer cell lines	IC₅₀	0.13 μM Compound: 1	Cytotoxicity against human pancreatic cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay Cytotoxicity against human pancreatic cancer cells assessed as growth inhibition after 72 hrs by Cell Titer Glo Assay	[PMID: 26272032]
CWR22R	IC₅₀	1.61 μM Compound: 1	Antiproliferative activity against human AR-positive 22Rv1 cells assessed as cell growth inhibition incubated for 3 days by WST-1 assay Antiproliferative activity against human AR-positive 22Rv1 cells assessed as cell growth inhibition incubated for 3 days by WST-1 assay	[PMID: 35772635]
DLD-1	IC₅₀	2.39 μM Compound: Niclosamide	Antiproliferative activity against human DLD1 cells after 72 hrs by colorimetric MTS assay Antiproliferative activity against human DLD1 cells after 72 hrs by colorimetric MTS assay	[PMID: 30274939]
DU-145	IC₅₀	0.7 μM Compound: 5	Antiproliferative activity against human DU145 cells after 72 hrs by MTT assay Antiproliferative activity against human DU145 cells after 72 hrs by MTT assay	[PMID: 24900231]
DU-145	IC₅₀	0.7 μM Compound: 5	Growth inhibition of human DU145 cells assessed as inhibition of colony formation after 11 to 12 days by crystal violet staining based microscopic analysis Growth inhibition of human DU145 cells assessed as inhibition of colony formation after 11 to 12 days by crystal violet staining based microscopic analysis	[PMID: 24900231]
DU-145	IC₅₀	3.65 μM Compound: 1	Antiproliferative activity against human AR-negative DU-145 cells assessed as cell growth inhibition incubated for 3 days by WST-1 assay Antiproliferative activity against human AR-negative DU-145 cells assessed as cell growth inhibition incubated for 3 days by WST-1 assay	[PMID: 35772635]
HCT-116	EC₅₀	0.056 μM Compound: 1	Effect on ATP homeostasis in human HCT116 cells assessed as reduction in ATP level after 3 hrs in absence of glucose by ATP bioluminescent assay Effect on ATP homeostasis in human HCT116 cells assessed as reduction in ATP level after 3 hrs in absence of glucose by ATP bioluminescent assay	[PMID: 28233680]
HCT-116	IC₅₀	0.25 μM Compound: 1	Effect on ATP homeostasis in human HCT116 cells assessed as reduction in ATP level after 3 hrs in absence of glucose by ATP bioluminescent assay Effect on ATP homeostasis in human HCT116 cells assessed as reduction in ATP level after 3 hrs in absence of glucose by ATP bioluminescent assay	[PMID: 28233680]
HCT-116	IC₅₀	0.41 μM Compound: Niclosamide	Antiproliferative activity against human HCT116 cells after 72 hrs by colorimetric MTS assay Antiproliferative activity against human HCT116 cells after 72 hrs by colorimetric MTS assay	[PMID: 30274939]
HCT-116	IC₅₀	0.45 μM Compound: 1	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTS assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTS assay	[PMID: 26272032]
HCT-116	IC₅₀	0.45 μM Compound: Niclosamide	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	[PMID: 33774344]
HEK293	IC₅₀	0.34 μM Compound: 1	Inhibition of Wnt3A-stimulated Wnt/beta-catenin pathway in HEK293 cells transfected with p8xTOPFlash, Renilla luciferase plasmid pRL-TK and pLKO.1 after 8 hrs by Dual topflash luciferase reporter gene assay Inhibition of Wnt3A-stimulated Wnt/beta-catenin pathway in HEK293 cells transfected with p8xTOPFlash, Renilla luciferase plasmid pRL-TK and pLKO.1 after 8 hrs by Dual topflash luciferase reporter gene assay	[PMID: 30551901]
HEK293	IC₅₀	0.34 μM Compound: 1	Inhibition of Wnt/beta-catenin in HEK293 cells assessed as inhibition of Wnt3A-stimulated TOPFlash activity after 8 hrs Inhibition of Wnt/beta-catenin in HEK293 cells assessed as inhibition of Wnt3A-stimulated TOPFlash activity after 8 hrs	[PMID: 26272032]
HEK293	IC₅₀	0.4 μM Compound: Niclosamide	Inhibition of Wnt3A/beta-casein signaling in HEK293 cells after 8 hrs by TOPflash reporter assay Inhibition of Wnt3A/beta-casein signaling in HEK293 cells after 8 hrs by TOPflash reporter assay	[PMID: 23453073]
HEK293	IC₅₀	1.53 μM Compound: 89	Inhibition of KIX-RLucN fused CBP (unknown origin) binding to KID-RLucC fused CREB (unknown origin) transfected in human HEK293 cells preincubated with compound 30 mins before before forskolin addition and measured after 90 mins in presence of coelenteraz Inhibition of KIX-RLucN fused CBP (unknown origin) binding to KID-RLucC fused CREB (unknown origin) transfected in human HEK293 cells preincubated with compound 30 mins before before forskolin addition and measured after 90 mins in presence of coelenteraz	[PMID: 31253529]
HeLa	IC₅₀	0.25 μM Compound: 5	Inhibition of STAT3 in human HeLa cells after 24 hrs by luciferase reporter gene assay Inhibition of STAT3 in human HeLa cells after 24 hrs by luciferase reporter gene assay	[PMID: 24900231]
HeLa	IC₅₀	1.4 μM Compound: 5	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 24900231]
HepG2 2.2.15	CC₅₀	> 100 μM Compound: Niclosamide	Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay	[PMID: 21553812]
HL-60	IC₅₀	0.3 μM Compound: 89	Cytotoxicity against human HL60 cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human HL60 cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31253529]
HT-29	IC₅₀	0.13 μM Compound: Niclosamide	Antiproliferative activity against human HT-29 cells after 72 hrs by colorimetric MTS assay Antiproliferative activity against human HT-29 cells after 72 hrs by colorimetric MTS assay	[PMID: 30274939]
HT-29	IC₅₀	7.2 μM Compound: 5	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 24900231]
Jurkat	IC₅₀	0.4 μM Compound: 89	Cytotoxicity against human Jurkat cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human Jurkat cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31253529]
KG-1	IC₅₀	0.36 μM Compound: 89	Cytotoxicity against human KG1 cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human KG1 cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31253529]
LN-229	IC₅₀	0.3 μM Compound: 77	Antiproliferative activity against human LN229 cells after 5 days by AlamarBlue assay Antiproliferative activity against human LN229 cells after 5 days by AlamarBlue assay	[PMID: 30583248]
LNCaP	IC₅₀	0.8 μM Compound: 1	Antiproliferative activity against human AR-positive LNCaP cells assessed as cell growth inhibition incubated for 3 days by WST-1 assay Antiproliferative activity against human AR-positive LNCaP cells assessed as cell growth inhibition incubated for 3 days by WST-1 assay	[PMID: 35772635]
LoVo	IC₅₀	0.7 μM Compound: Niclosamide	Antiproliferative activity against human LoVo cells after 120 hrs by MTT assay Antiproliferative activity against human LoVo cells after 120 hrs by MTT assay	[PMID: 16680159]
MCF7	IC₅₀	1.06 μM Compound: 1	Cytotoxicity against human ER positive MCF7 cells after 72 hrs by MTS assay Cytotoxicity against human ER positive MCF7 cells after 72 hrs by MTS assay	[PMID: 23459613]
MCF7	IC₅₀	1.06 μM Compound: Niclosamide	CYtotoxicity against ER-positive human MCF7 cells by MTS assay CYtotoxicity against ER-positive human MCF7 cells by MTS assay	[PMID: 23416191]
MCF7	IC₅₀	8.13 μM Compound: 1	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 35334447]
MDA-MB-231	IC₅₀	0.625 μM Compound: 27	Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay	[PMID: 33650861]
MDA-MB-231	IC₅₀	0.79 μM Compound: 1	Cytotoxicity against human ER negative MDA-MB-231 cells after 72 hrs by MTS assay Cytotoxicity against human ER negative MDA-MB-231 cells after 72 hrs by MTS assay	[PMID: 23459613]
MDA-MB-231	IC₅₀	0.79 μM Compound: Niclosamide	CYtotoxicity against ER-negative human MDA-MB-231 cells by MTS assay CYtotoxicity against ER-negative human MDA-MB-231 cells by MTS assay	[PMID: 23416191]
MDA-MB-231	IC₅₀	0.8 μM Compound: Niclosamide	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	[PMID: 33774344]
MDA-MB-231	IC₅₀	1.78 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay	[PMID: 35334447]
MIA PaCa-2	IC₅₀	1.1 μM Compound: Niclosamide	Antiproliferative activity against human MIAPaCa2cells after 120 hrs by MTT assay Antiproliferative activity against human MIAPaCa2cells after 120 hrs by MTT assay	[PMID: 16680159]
NALM-6	IC₅₀	0.62 μM Compound: 89	Cytotoxicity against human NALM6 cells incubated for 3 days by CellTiter-Glo luminescent assay Cytotoxicity against human NALM6 cells incubated for 3 days by CellTiter-Glo luminescent assay	[PMID: 31253529]
PANC-1	IC₅₀	1.73 μM Compound: 1	Cytotoxicity against human PANC1 cells after 72 hrs by MTS assay Cytotoxicity against human PANC1 cells after 72 hrs by MTS assay	[PMID: 23459613]
PANC-1	IC₅₀	1.73 μM Compound: Niclosamide	CYtotoxicity against human PANC1 cells by MTS assay CYtotoxicity against human PANC1 cells by MTS assay	[PMID: 23416191]
PC-3	IC₅₀	0.4 μM Compound: Niclosamide	Antiproliferative activity against human PC3 cells after 120 hrs by MTT assay Antiproliferative activity against human PC3 cells after 120 hrs by MTT assay	[PMID: 16680159]
PC-3	IC₅₀	1.92 μM Compound: 1	Antiproliferative activity against human AR-negative PC-3 cells assessed as cell growth inhibition incubated for 3 days by WST-1 assay Antiproliferative activity against human AR-negative PC-3 cells assessed as cell growth inhibition incubated for 3 days by WST-1 assay	[PMID: 35772635]
PC-3	IC₅₀	11.7 μM Compound: 5	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 24900231]
SH-SY5Y	IC₅₀	0.87 μM Compound: Niclosamide	Anticancer activity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based fluorescence assay Anticancer activity against human SH-SY5Y cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based fluorescence assay	[PMID: 36208544]
SK-N-AS	IC₅₀	2.33 μM Compound: Niclosamide	Anticancer activity against human SK-N-AS cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based fluorescence assay Anticancer activity against human SK-N-AS cells assessed as reduction in cell viability incubated for 48 hrs by resazurin dye based fluorescence assay	[PMID: 36208544]
SW480	IC₅₀	0.98 μM Compound: Niclosamide	Antiproliferative activity against human SW480 cells after 72 hrs by colorimetric MTS assay Antiproliferative activity against human SW480 cells after 72 hrs by colorimetric MTS assay	[PMID: 30274939]
SW480	IC₅₀	1 μM Compound: 93	Anticancer activity against human SW480 cells assessed as reduction in cell viability incubated for 12 to 24 hrs by MTS assay Anticancer activity against human SW480 cells assessed as reduction in cell viability incubated for 12 to 24 hrs by MTS assay	[PMID: 33445154]
SW948	IC₅₀	0.11 μM Compound: Niclosamide	Antiproliferative activity against human SW948 cells after 72 hrs by colorimetric MTS assay Antiproliferative activity against human SW948 cells after 72 hrs by colorimetric MTS assay	[PMID: 30274939]
T98G	IC₅₀	0.3 μM Compound: 77	Antiproliferative activity against human T98G cells after 5 days by AlamarBlue assay Antiproliferative activity against human T98G cells after 5 days by AlamarBlue assay	[PMID: 30583248]
U138-MG	IC₅₀	0.3 μM Compound: 77	Antiproliferative activity against human U138MG cells after 5 days by AlamarBlue assay Antiproliferative activity against human U138MG cells after 5 days by AlamarBlue assay	[PMID: 30583248]
U2OS	CC₅₀	> 20 μM Compound: 104	Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay	[PMID: 28689975]
U-373MG ATCC	IC₅₀	0.3 μM Compound: 77	Antiproliferative activity against human U373 cells after 5 days by AlamarBlue assay Antiproliferative activity against human U373 cells after 5 days by AlamarBlue assay	[PMID: 30583248]
U-87MG ATCC	IC₅₀	0.3 μM Compound: 77	Antiproliferative activity against human U87 cells after 5 days by AlamarBlue assay Antiproliferative activity against human U87 cells after 5 days by AlamarBlue assay	[PMID: 30583248]
U-87MG ATCC	IC₅₀	0.4 μM Compound: Niclosamide	Antiproliferative activity against human U87MG cells after 120 hrs by MTT assay Antiproliferative activity against human U87MG cells after 120 hrs by MTT assay	[PMID: 16680159]
Vero	EC₅₀	< 0.1 μM Compound: 21	Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA	[PMID: 17663539]
Vero	CC₅₀	> 50 μM Compound: Niclosamide	Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr	10.1101/2020.03.20.999730
Vero	IC₅₀	0.28 μM Compound: Niclosamide	Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr	10.1101/2020.03.20.999730
Vero	CC₅₀	22.1 μM Compound: 21	Cytotoxicity against Vero E6 cells by MTT assay Cytotoxicity against Vero E6 cells by MTT assay	[PMID: 17663539]
Vero C1008	CC₅₀	> 35.53 μM Compound: Niclosamide	Cytotoxicity against African green monkey Vero E6 cells assessed as reduction of cell viability measured after 48 hrs by CCK-8 assay Cytotoxicity against African green monkey Vero E6 cells assessed as reduction of cell viability measured after 48 hrs by CCK-8 assay	[PMID: 32563814]
Vero C1008	CC₅₀	1.03 μM Compound: 1	Cytotoxicity against African Green monkey Vero E6 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against African Green monkey Vero E6 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 35344901]
Vero C1008	CC₅₀	100 μM Compound: 1	Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability preincubated for 2 hrs followed by SARS-CoV-2 addition measured after 24 hrs by CPE assay Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability preincubated for 2 hrs followed by SARS-CoV-2 addition measured after 24 hrs by CPE assay	[PMID: 35344901]

体外研究
(In Vitro)

Niclosamide (0.6 nM-46 μM) 处理可抑制 BD140A、SW-13 和 NCI-H295R 细胞中的肾上腺皮质癌细胞增殖^[3]。
Niclosamide (0.05-5 μM，24 h) 处理抑制 HeLa 细胞中 STAT3 介导的荧光素酶报告基因活性^[4]。
Niclosamide (10 μM) 处理抑制 Vero E6 细胞中的病毒复制^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	BD140A, SW-13 and NCI-H295R cells
Concentration:	0.6 nM-46 µM
Incubation Time:
Result:	Inhibited cellular proliferation in adrenocortical carcinoma cell lines with the IC₅₀ of 0.12 µM, 0.15 µM, and 0.53 µM in BD140A, SW-13, and NCI-H295R, respectively.

Cell Viability Assay^[4]

Cell Line:	Hela cells
Concentration:	0.05-5 μM
Incubation Time:	24 hours
Result:	Inhibited STAT3-mediated luciferase reporter activity with an IC₅₀ of 0.25 μM.

Cell Viability Assay^[4]

Cell Line:	Vero E6 cells^[5]
Concentration:	10 μM
Incubation Time:	2 days
Result:	Inhibited the synthesis of viral antigens of SARS-CoV in Vero E6 cells.

体内研究
(In Vivo)

Niclosamide (口服灌胃；100 mg/kg、200 mg/kg；每周一次；8 周) 处理抑制体内肾上腺皮质癌肿瘤生长^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nu⁺/Nu⁺ mice injected with NCI-H295R cells^[3]
Dosage:	100 mg/kg, 200 mg/kg
Administration:	Oral gavage; 100 mg/kg, 200 mg/kg; once a week; 8 weeks
Result:	Showed a 60%-80% inhibition in tumor growth, as compared to the control group.

Clinical Trial

分子量

327.12

Formula

C₁₃H₈Cl₂N₂O₄

CAS 号

50-65-7

性状

固体

颜色

Light yellow to green yellow

中文名称

氯硝柳胺；杀螺胺

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

溶解性数据

细胞实验：

DMF 中的溶解度 : 5 mg/mL (15.28 mM; 超声助溶)

DMSO 中的溶解度 : 4.55 mg/mL (13.91 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.0570 mL	15.2849 mL	30.5698 mL
5 mM	0.6114 mL	3.0570 mL	6.1140 mL
10 mM	0.3057 mL	1.5285 mL	3.0570 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMF 40% PEG300 5% Tween-80 45% Saline
Solubility: 0.5 mg/mL (1.53 mM); 悬浊液; 超声助溶

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： 15% Cremophor EL 85% Saline
Solubility: 20 mg/mL (61.14 mM); 悬浊液; 超声助溶
方案二

请依序添加每种溶剂： 5% Cremophor EL 95% (20% HP-β-CD in Saline)
Solubility: 5 mg/mL (15.28 mM); 悬浊液; 超声助溶
方案三

请依序添加每种溶剂： 10% Cremophor EL in PBS
Solubility: 3 mg/mL (9.17 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.90%

选择批次：

Data Sheet (630 KB) SDS (598 KB)

COA (192 KB) HNMR (275 KB) LCMS (270 KB)

产品使用指南 (1538 KB)

参考文献

[1]. P Andrews, et al. The biology and toxicology of molluscicides, Bayluscide. Pharmacol Ther. 1982;19(2):245-95. [Content Brief]

[2]. Wei Chen, et al. Niclosamide: Beyond an antihelminthic drug. Cell Signal. 2018 Jan;41:89-96. [Content Brief]

[3]. Kei Satoh, et al. Identification of Niclosamide as a Novel Anticancer Agent for Adrenocortical Carcinoma. Clin Cancer Res. 2016 Jul 15;22(14):3458-66. [Content Brief]

[4]. Xiaomei Ren, et al. Identification of Niclosamide as a New Small-Molecule Inhibitor of the STAT3 Signaling Pathway. ACS Med Chem Lett. 2010 Sep 7;1(9):454-9. [Content Brief]

[5]. Chang-Jer Wu, et al. Inhibition of severe acute respiratory syndrome coronavirus replication by niclosamide. Antimicrob Agents Chemother. 2004 Jul;48(7):2693-6. [Content Brief]

Niclosamide 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO / DMF	1 mM	3.0570 mL	15.2849 mL	30.5698 mL	76.4246 mL
	5 mM	0.6114 mL	3.0570 mL	6.1140 mL	15.2849 mL
	10 mM	0.3057 mL	1.5285 mL	3.0570 mL	7.6425 mL
DMF	15 mM	0.2038 mL	1.0190 mL	2.0380 mL	5.0950 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Niclosamide (Synonyms: 氯硝柳胺; BAY2353)

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Niclosamide (Synonyms: 氯硝柳胺; BAY2353)

Customer Review

Other Forms of Niclosamide:

Niclosamide 相关抗体

MCE 顾客使用本产品发表的 27 篇科研文献

Niclosamide 相关产品

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