2. NF-κB Autophagy Apoptosis Anti-infection
  3. NF-κB Autophagy Apoptosis Ferroptosis Bacterial Antibiotic
  4. Sulfasalazine

Sulfasalazine  (Synonyms: 柳氮磺吡啶; NSC 667219)

目录号: HY-14655 纯度: 98.75%
COA 产品使用指南

摩尔计算器

Sulfasalazine (NSC 667219) 常用于类风湿关节炎和溃疡性结肠炎的相关研究。Sulfasalazine 可抑制 NF-κB 的活性。Sulfasalazine 是 type 1 的铁死亡 (ferroptosis) 诱导剂。

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Sulfasalazine Chemical Structure

Sulfasalazine Chemical Structure

CAS No. : 599-79-1

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Customer Review

Other Forms of Sulfasalazine:

Sulfasalazine 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 38 篇科研文献

WB

    Sulfasalazine purchased from MCE. Usage Cited in: Mol Ther. 2021 Mar 17;S1525-0016(21)00142-8.  [Abstract]

    PD-L1 expression is significantly increased after Sulfasalazine (SAS) treatment at both the protein and mRNA levels.

    Sulfasalazine purchased from MCE. Usage Cited in: Cell Res. 2018 Dec;28(12):1171-1185.  [Abstract]

    Melanoma A375 cells are treated with SSZ (Sulfasalazine, 125 μM) with or without FeSO4 (100 μM) for 6 h to detect the ROS level or 24 h to assess the pyroptotic features (including morphology, GSDME cleavage, and LDH release), unless specifically defined.

    查看 NF-κB 亚型特异性产品:

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    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Sulfasalazine (NSC 667219) is an anti-rheumatic agent for the research of rheumatoid arthritis and ulcerative colitis. Sulfasalazine can suppress NF-κB activity. Sulfasalazine is a type 1 ferroptosis inducer[1][2][3][4].

    IC50 & Target[1]

    RelA

     

    Autophagy

     

    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    HEK293 IC50
    0.56 μM
    Compound: Sulfasalazine
    Inhibition of human liver OATP1B1 expressed in HEK293 Flp-In cells assessed as reduction in E17-betaG uptake by scintillation counting
    Inhibition of human liver OATP1B1 expressed in HEK293 Flp-In cells assessed as reduction in E17-betaG uptake by scintillation counting
    		[PMID: 22541068]
    HEK293 IC50
    12 μM
    Compound: Sulfasalazine
    Inhibition of human liver OATP1B3 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E17-betaG uptake incubated for 5 mins by scintillation counting
    Inhibition of human liver OATP1B3 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E17-betaG uptake incubated for 5 mins by scintillation counting
    		[PMID: 22541068]
    HEK293 IC50
    3 μM
    Compound: Sulfasalazine
    Inhibition of human liver OATP2B1 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E3S uptake incubated for 5 mins by scintillation counting
    Inhibition of human liver OATP2B1 expressed in HEK293 Flp-In cells assessed as reduction in [3H]E3S uptake incubated for 5 mins by scintillation counting
    		[PMID: 22541068]
    HepG2 IC50
    0.9 μM
    Compound: SFZ
    Inhibition of TNF-alpha-induced NFkappaB activation in human HepG2 cells after 1 hr by luciferase reporter assay
    Inhibition of TNF-alpha-induced NFkappaB activation in human HepG2 cells after 1 hr by luciferase reporter assay
    		[PMID: 21870831]
    HepG2 IC50
    0.9 μM
    Compound: Sulfasalazine
    Inhibition of TNF-alpha-induced NF-kappaB transcriptional activation in human HepG2 cells preincubated for 1 hr followed by TNF-alpha challenge measured after 1 hr by luciferase reporter gene assay
    Inhibition of TNF-alpha-induced NF-kappaB transcriptional activation in human HepG2 cells preincubated for 1 hr followed by TNF-alpha challenge measured after 1 hr by luciferase reporter gene assay
    		[PMID: 24314396]
    HepG2 IC50
    0.9 μM
    Compound: SFZ
    Inhibition of NF-kappaB expressed in human HepG2 cells assessed as inhibition of TNFalpha-induced luciferase activity preincubated for 1 hr followed by TNFalpha challenge measured after 1 hr by reporter gene assay
    Inhibition of NF-kappaB expressed in human HepG2 cells assessed as inhibition of TNFalpha-induced luciferase activity preincubated for 1 hr followed by TNFalpha challenge measured after 1 hr by reporter gene assay
    		[PMID: 23031596]
    HepG2 IC50
    0.9 μM
    Compound: Sulfasalazine
    Inhibition of TNFalpha-induced NFkappaB transcriptional activity in human HepG2 cells pretreated for 1 hr before TNFalpha challenge by luciferase reporter gene analysis
    Inhibition of TNFalpha-induced NFkappaB transcriptional activity in human HepG2 cells pretreated for 1 hr before TNFalpha challenge by luciferase reporter gene analysis
    		[PMID: 22381047]
    HepG2 IC50
    0.9 μM
    Compound: Sulfasalazine
    Inhibition of NF-kappaB activation expressed in TNFalpha-stimulated human HepG2 cells co-transfected with PPRE-Luc plasmid pretreated for 1 hr before TNFalpha challenge measured after 24 hrs by luciferase reporter gene assay
    Inhibition of NF-kappaB activation expressed in TNFalpha-stimulated human HepG2 cells co-transfected with PPRE-Luc plasmid pretreated for 1 hr before TNFalpha challenge measured after 24 hrs by luciferase reporter gene assay
    		[PMID: 21889336]
    T-cell IC50
    > 10 μM
    Compound: Salazosulfapyridine
    Inhibitory concentration against T cell proliferation using concanavalin A stimulated mice spleen cells
    Inhibitory concentration against T cell proliferation using concanavalin A stimulated mice spleen cells
    		[PMID: 11229767]
    U2OS IC50
    9.6 μM
    Compound: Sulfasalazine
    Inhibition of HA-tagged human NTCP expressed in human U2OS cells assessed as reduction in [14C]taurocholate uptake preincubated for 10 mins followed by [14C] taurocholate addition and further incubation for 10 mins by scintillation counting method
    Inhibition of HA-tagged human NTCP expressed in human U2OS cells assessed as reduction in [14C]taurocholate uptake preincubated for 10 mins followed by [14C] taurocholate addition and further incubation for 10 mins by scintillation counting method
    		[PMID: 31439379]
    U-87MG ATCC IC50
    30 μM
    Compound: 2
    Inhibition of xc-cystine-glutamate antiporter-mediated cystine uptake in human U87 cells using L-[14C]cystine as substrate after 15 mins by liquid scintillation counting
    Inhibition of xc-cystine-glutamate antiporter-mediated cystine uptake in human U87 cells using L-[14C]cystine as substrate after 15 mins by liquid scintillation counting
    		[PMID: 21889337]
    体外研究
    (In Vitro)

    用柳氮磺吡啶处理 SW620 结肠细胞可抑制 TNFα、LPS 或佛波醇酯诱导的 NFκB 活化。柳氮磺吡啶在微摩尔至毫摩尔浓度下可抑制 NFκB 依赖性转录。柳氮磺吡啶通过抑制 IκBα 降解来阻止 TNFα 诱导的 NFκB 核转位[1]。仅用 5 mM 柳氮磺吡啶预孵育可显著增加所有促炎细胞因子的基础 mRNA 表达，IL-6 mRNA 水平与载体对照相比增加 80 倍[2]。消化后，柳氮磺吡啶被结肠细菌裂解为磺胺吡啶和 5-氨基水杨酸，据报道后者也会抑制 NF-κB 活性[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Sulfasalazine 相关抗体:
    体内研究
    (In Vivo)

    与未经治疗的对照组相比，Sulfasalazine (0.25 mM) 能够抑制超过 60% 的胶质瘤生长[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    398.39

    Formula

    C18H14N4O5S
    CAS 号
    性状

    固体

    颜色

    Light yellow to orange

    中文名称

    柳氮磺吡啶；水扬酸偶氮磺胺毗咙
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性数据
    细胞实验： 

    NH4OH 中的溶解度 : 150 mg/mL (376.52 mM; 超声助溶; 碱性条件溶解 ( NH4OH 调节，pH≈9))

    1 M NaOH 中的溶解度 : ≥ 100 mg/mL (251.01 mM)

    DMSO 中的溶解度 : 80 mg/mL (200.81 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.5101 mL 12.5505 mL 25.1010 mL
    5 mM 0.5020 mL 2.5101 mL 5.0202 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.28 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.28 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： 50% PEG300    50% Saline

      Solubility: 10 mg/mL (25.10 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.04%

    COA (194 KB) LCMS (268 KB)

    产品使用指南 (1538 KB)
    参考文献
    Kinase Assay

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [1]

    Sulfasalazine is dissolved in culture medium. SW620 cells are grown in Dulbecco’s modified Eagle medium, supplemented with 10% heat-inactivated FCS, 2 mmol/liter glutamine, and 1% (wt/vol) penicillin/streptomycin. SW620 cells are transfected with the 3xIgkBLuc reporter construct. After 18 h, cells are incubated with either medium alone or with sulfasalazine (0.1, 0.2, 0.5, 1, 2, 5 mM) before stimulation with TNFα, LPS, or PMA. Luciferase assay is performed[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    Mice: Sulfasalazine is dissolved in 0.1 M NaOH, and then neutralized by titrating with 0.1 M HCl. U-87MG glioma cells are implanted into the cranium of a SCID mouse. After 7 days, animals are randomized into three groups of five animals each. One group receives 1 mL i.p. saline injections twice daily for 3 weeks. The two test groups receives 8 mg of sulfasalazine in 1 mL saline twice daily for 3 weeks. Tumor growth and animal health were monitored. After perfusion with 4% paraformaldehyde, mouse brains were collected, rinsed, and placed in 30% sucrose[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Sulfasalazine 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / 1 M NaOH / NH4OH 1 mM 2.5101 mL 12.5505 mL 25.1010 mL 62.7526 mL
    5 mM 0.5020 mL 2.5101 mL 5.0202 mL 12.5505 mL
    10 mM 0.2510 mL 1.2551 mL 2.5101 mL 6.2753 mL
    15 mM 0.1673 mL 0.8367 mL 1.6734 mL 4.1835 mL
    20 mM 0.1255 mL 0.6275 mL 1.2551 mL 3.1376 mL
    25 mM 0.1004 mL 0.5020 mL 1.0040 mL 2.5101 mL
    30 mM 0.0837 mL 0.4184 mL 0.8367 mL 2.0918 mL
    40 mM 0.0628 mL 0.3138 mL 0.6275 mL 1.5688 mL
    50 mM 0.0502 mL 0.2510 mL 0.5020 mL 1.2551 mL
    60 mM 0.0418 mL 0.2092 mL 0.4184 mL 1.0459 mL
    80 mM 0.0314 mL 0.1569 mL 0.3138 mL 0.7844 mL
    100 mM 0.0251 mL 0.1255 mL 0.2510 mL 0.6275 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sulfasalazine599-79-1NSC 667219柳氮磺吡啶水扬酸偶氮磺胺毗咙NSC667219NSC-667219NF-κBAutophagyApoptosisFerroptosisBacterialAntibioticNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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