2. Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Autophagy
  3. PPAR Autophagy
  4. Eupatilin

Eupatilin  (Synonyms: 异泽兰黄素)

目录号: HY-N0783 纯度: 98.90%
COA 产品使用指南

摩尔计算器

Eupatilin 是从艾叶中分到的亲脂性黄酮类化合物，是 PPARα 的激动剂，具有抗凋亡，抗氧化及抗炎等功效。

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Eupatilin Chemical Structure

Eupatilin Chemical Structure

CAS No. : 22368-21-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥847
In-stock
5 mg ￥766
In-stock
10 mg ￥1200
In-stock
25 mg ￥2400
In-stock
50 mg ￥4150
In-stock
100 mg ￥5810
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Eupatilin:

Eupatilin 相关抗体

Top Publications Citing Use of Products

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PPARα PPARβ/δ PPARγ PPAR PPARδ

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Eupatilin, a lipophilic flavonoid isolated from Artemisia argyi Lévl. et Van., is a PPARα agonist, and possesses anti-apoptotic, anti-oxidative and anti-inflammatory activities.

IC50 & Target[1]

PPARα

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
> 100 μM
Compound: 6
Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
[PMID: 27400887]
AGS IC50
0.5 x 10-4 M
Compound: Eupatilin
Antiproliferative activity against human AGS cells assessed as reduction in cell viability after 24 hrs by CCK8 assay
Antiproliferative activity against human AGS cells assessed as reduction in cell viability after 24 hrs by CCK8 assay
[PMID: 31784199]
Bcap37 IC50
> 100 μM
Compound: 6
Cytotoxicity against human Bcap37 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
Cytotoxicity against human Bcap37 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
[PMID: 27400887]
HepG2 IC50
52.65 μM
Compound: 6
Cytotoxicity against human HepG2 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
[PMID: 27400887]
MCF7 IC50
90.07 μM
Compound: 6
Cytotoxicity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
[PMID: 27400887]
MKN-1 IC50
0.5 x 10-4 M
Compound: Eupatilin
Antiproliferative activity against human MKN1 cells assessed as reduction in cell viability after 24 hrs by CCK8 assay
Antiproliferative activity against human MKN1 cells assessed as reduction in cell viability after 24 hrs by CCK8 assay
[PMID: 31784199]
SNU-638 IC50
0.5 x 10-4 M
Compound: Eupatilin
Antiproliferative activity against human SNU638 cells assessed as reduction in cell viability after 24 hrs by CCK8 assay
Antiproliferative activity against human SNU638 cells assessed as reduction in cell viability after 24 hrs by CCK8 assay
[PMID: 31784199]
T-cell IC50
11.7 μM
Compound: 16
Immunosuppressant activity in human T lymphocytes assessed as inhibition of anti-human CD3 and anti-human CD28 monoclonal antibody-induced T lymphocyte proliferation incubated for 72 hrs by FACS analysis
Immunosuppressant activity in human T lymphocytes assessed as inhibition of anti-human CD3 and anti-human CD28 monoclonal antibody-induced T lymphocyte proliferation incubated for 72 hrs by FACS analysis
[PMID: 31181920]
U-251 IC50
> 100 μM
Compound: 6
Cytotoxicity against human U251 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
Cytotoxicity against human U251 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay
[PMID: 27400887]
体外研究
(In Vitro)

Eupatilin 是一种 PPARα 激动剂。Eupatilin (10，30，100 μM) 抑制 RBL-2H3 细胞中的 IL-4 表达和脱颗粒[1]。
Eupatilin (50-100 μM) 轻微降低 HaCaT 细胞的细胞活力。Eupatilin (10，30，50，100 μM) 在 HaCaT 细胞中增加 PPARα 反式激活和表达。Eupatilin (10，30，50 μM) 也抑制 HaCaT 细胞中 TNFα 诱导的 MMP-2/-9 表达。此外，Eupatilin 通过 PPARα 抑制 TNFα 诱导的 p65 易位、IκBα 磷酸化、AP-1 和 MAPK 信号转导[2]。
Eupatilin (10-50 μM) 对 ARPE19 细胞没有细胞毒性作用。Eupatilin (10，25，50 μM) 提高氧化应激引起的细胞活力，并抑制 ARPE19 细胞中 H2O2 诱导的 ROS 产生。此外，Eupatilin (50 μM) 抑制H2O2诱导的细胞凋亡，并促进RPE细胞中PI3K/Akt通路的激活[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Eupatilin 相关抗体:
体内研究
(In Vivo)

Eupatilin (1.5% 或 3.0%) 可恢复 PPARα mRNA 表达，并改善恶唑酮诱导的 Balb/c 小鼠的特应性皮炎 (AD) 样症状。在恶唑酮诱导的小鼠中，Eupatilin 导致血清 IgE、IL-4 水平、恶唑酮诱导的 TNFα、IFNγ、IL-1β、TSLP、IL-33 和 IL-25 mRNA 表达显著降低。Eupatilin 还增加了恶唑酮诱导的小鼠中聚丝蛋白和兜甲蛋白的 mRNA 表达[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

344.32

Formula

C18H16O7
CAS 号
性状

固体

颜色

Light yellow to yellow

中文名称

异泽兰黄素
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 33.33 mg/mL (96.80 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9043 mL 14.5214 mL 29.0428 mL
5 mM 0.5809 mL 2.9043 mL 5.8085 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.26 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.26 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 98.90%

COA (298 KB) HNMR (249 KB) LCMS (96 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[3]

Cell viability is detected using a MTT assay. In brief, after treatment, the medium is replaced with fresh medium containing 0.5 mg/mL MTT for 4 h at 37°C. Then, the medium is gently aspirated and 150 μL of DMSO is added to each well to solubilize the formazan crystals. The absorbance is measured at 450 nm by a microplate reader. The relative cell viability is defined as the absorbance of treated wells divided by that of the control[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Six-week-old female Balb/c mice are housed under conditions of controlled temperature (23  ±  2 °C), humidity (55  ±  5%), and 12 h light/dark cycles (06:00-18:00 h light, 18:00-06:00 dark). Briefly, Balb/c mice are sensitized on day −7 by a single application of 20 μL of 1.0% oxazolone in a mixture of acetone and olive oil (4:1) to the inner and outer surface of both ears. On day 0, the mouse ears are challenged with 20 μL of 0.1% oxazolone at 2-day intervals for 4 weeks post-sensitization. The mice are treated with the indicated concentrations of Eupatilin (1.5% or 3.0%) twice a day for 4 weeks. The control group is treated with vehicle alone (acetone and olive oil [4:1]). After 3 weeks, the mice are sacrificed and samples are collected. Ears are stored at −80 °C for RNA isolation and analysis or immediately fixed in 4% formalin for histological analysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Eupatilin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9043 mL 14.5214 mL 29.0428 mL 72.6069 mL
5 mM 0.5809 mL 2.9043 mL 5.8086 mL 14.5214 mL
10 mM 0.2904 mL 1.4521 mL 2.9043 mL 7.2607 mL
15 mM 0.1936 mL 0.9681 mL 1.9362 mL 4.8405 mL
20 mM 0.1452 mL 0.7261 mL 1.4521 mL 3.6303 mL
25 mM 0.1162 mL 0.5809 mL 1.1617 mL 2.9043 mL
30 mM 0.0968 mL 0.4840 mL 0.9681 mL 2.4202 mL
40 mM 0.0726 mL 0.3630 mL 0.7261 mL 1.8152 mL
50 mM 0.0581 mL 0.2904 mL 0.5809 mL 1.4521 mL
60 mM 0.0484 mL 0.2420 mL 0.4840 mL 1.2101 mL
80 mM 0.0363 mL 0.1815 mL 0.3630 mL 0.9076 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Eupatilin22368-21-4异泽兰黄素PPARAutophagyPeroxisome proliferator-activated receptorsInhibitorinhibitorinhibit

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