2. Metabolic Enzyme/Protease Cell Cycle/DNA Damage Immunology/Inflammation NF-κB Apoptosis Anti-infection
  3. Endogenous Metabolite CDK Reactive Oxygen Species Apoptosis Enterovirus Bacterial
  4. Lacto-N-fucopentaose I

Lacto-N-fucopentaose I  (Synonyms: LNFP I)

目录号: HY-W019806 纯度: 99.92%
COA 产品使用指南 技术支持

Lacto-N-fucopentaose I (LNFPI) 是一种人母乳寡糖 (HMO)，具有抗病毒和抗菌活性。Lacto-N-fucopentaose I 通过减少衣壳蛋白 VP1 来阻断病毒吸附，增加 CDK2、减少 cyclin E 恢复细胞周期 S 期的阻滞。Lacto-N-fucopentaose I 抑制病毒感染的细胞凋亡 (apoptosis)。Lacto-N-fucopentaose I 也抑制 ROS 的产生。Lacto-N-fucopentaose I 还可以调节肠道菌群影响免疫系统发育。

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Lacto-N-fucopentaose I Chemical Structure

Lacto-N-fucopentaose I Chemical Structure

CAS No. : 7578-25-8

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Lacto-N-fucopentaose I 相关抗体

Top Publications Citing Use of Products

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CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Lacto-N-fucopentaose I (LNFPI) is a human milk oligosaccharide (HMO), possessing antiviral and antibacterial activity. Lacto-N-fucopentaose I can reduce capsid protein VP1 to block virus adsorption, promote CDK2 and reduce cyclin E to recover cell cycle S phase block. Lacto-N-fucopentaose I inhibits ROS production and apoptosis in virus-infected cells. Lacto-N-fucopentaose I can also regulate intestinal microbiota to affect immune system development[1].

IC50 & Target

Human Endogenous Metabolite

 

CDK2/cyclinE

 

体外研究
(In Vitro)

Lacto-N-fucopentaose I (25-3200 μg/mL;48 h) exhibits certain cytotoxicity at 3200 μg/mL but no toxic reaction below 1600 μg/mL[1].
Lacto-N-fucopentaose I (25-1600 μg/mL; 14-18 h) can protect EV71-infected RD cells from death[1].
Lacto-N-fucopentaose I (100-400 μg/mL; 16 h) decreases markedly mRNA levels of VP1 and ROS production in EV71-infected RD cells at 400 μg/mL; leads to the recovery of EV71-induced S phase arrest in RD cells[1].
Lacto-N-fucopentaose I (100 and 200 μg/mL; 3 days) inhibits cell apoptosis in Caenorhabditis elegans; significantly decreases the levels of Egl-1, Ced-3 and Ced-4[1].
Lacto-N-fucopentaose I can reduce the abundance of Sphingobacterium, Stenotrophomonas and Achromobacter; can increase the abundance of Micromonospora, Vibrio, Acidibacter, Gaiella, Devosia, Steroidobacter, Variibacter, Dactylosporangium, RB41, Pir4_lineage, Pirellula, Haliangium, Roseiflexus, Pedomicrobium, and Bradyrhizobium.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Lacto-N-fucopentaose I 相关抗体:

Cell Viability Assay[1]

Cell Line: RD cells
Concentration: 25, 50, 100, 200, 400, 800, 1600 and 3200 μg/mL
Incubation Time: 48 h
Result: Exhibited certain cytotoxicity at 3200 μg/mL but no toxic reaction below 1600 μg/mL.

RT-PCR[1]

Cell Line: RD cells (infected with EV71)
Concentration: 100, 200 and 400 μg/mL
Incubation Time: 16 h
Result: Decreased markedly mRNA levels of VP1 only at 400 μg/mL.

Apoptosis Analysis[1]

Cell Line: RD cells (infected with EV71)
Concentration: 100, 200 and 400 μg/mL
Incubation Time: 16 h
Result: Decreased the rate of cells in early apoptosis to 10.9% ± 1.26% at 400 μg/mL, while the untreated EV71 group was 27.7% ± 2.13%.
Significantly inhibited the activity of caspase-3, caspase-8 and caspase-9.
Recovered the decreased mRNA expression of PAPR, NF-κB and Bcl-2 and properly regulated Bad and Fas into their normal levels.

Cell Cycle Analysis[1]

Cell Line: RD cells (infected with EV71)
Concentration: 100, 200 and 400 μg/mL
Incubation Time: 12 h
Result: Rescued EV71-induced S phase arrest, which promoted the transition of the G1 phase to the S phase.
分子量

853.77

Formula

C32H55NO25
CAS 号
性状

固体

颜色

White to off-white

结构分类
初始来源

human milk
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验： 

H2O 中的溶解度 : 125 mg/mL (146.41 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1713 mL 5.8564 mL 11.7128 mL
5 mM 0.2343 mL 1.1713 mL 2.3426 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.92%

COA (301 KB) HNMR (332 KB) RP-HPLC (240 KB) MS (80 KB)

产品使用指南 (1538 KB)
参考文献

Lacto-N-fucopentaose I 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.1713 mL 5.8564 mL 11.7128 mL 29.2819 mL
5 mM 0.2343 mL 1.1713 mL 2.3426 mL 5.8564 mL
10 mM 0.1171 mL 0.5856 mL 1.1713 mL 2.9282 mL
15 mM 0.0781 mL 0.3904 mL 0.7809 mL 1.9521 mL
20 mM 0.0586 mL 0.2928 mL 0.5856 mL 1.4641 mL
25 mM 0.0469 mL 0.2343 mL 0.4685 mL 1.1713 mL
30 mM 0.0390 mL 0.1952 mL 0.3904 mL 0.9761 mL
40 mM 0.0293 mL 0.1464 mL 0.2928 mL 0.7320 mL
50 mM 0.0234 mL 0.1171 mL 0.2343 mL 0.5856 mL
60 mM 0.0195 mL 0.0976 mL 0.1952 mL 0.4880 mL
80 mM 0.0146 mL 0.0732 mL 0.1464 mL 0.3660 mL
100 mM 0.0117 mL 0.0586 mL 0.1171 mL 0.2928 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lacto-N-fucopentaose I7578-25-8LNFP IEndogenous MetaboliteCDKReactive Oxygen SpeciesApoptosisEnterovirusBacterialCyclin dependent kinaseRhinovirusHRVHRVsHEVHEVsHMOVP1intestinal microbiotaRD cellsanti-inflammatoryInhibitorinhibitorinhibit

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