2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related Epigenetics Apoptosis Anti-infection
  3. Topoisomerase ADC Cytotoxin MicroRNA Influenza Virus Apoptosis Fungal Antibiotic
  4. Camptothecin

Camptothecin  (Synonyms: 喜树碱; Campathecin; (S)-(+)-Camptothecin; CPT)

目录号: HY-16560 纯度: 99.86%
COA 产品使用指南

摩尔计算器

Camptothecin (CPT)，一种生物碱，是一种 DNA topoisomerase I (Topo I) 抑制剂，其 IC50 值为 679 nM。Camptothecin (CPT) 对结直肠癌、乳腺癌、肺癌和卵巢癌具有强大的抗肿瘤活性，通过改变人类癌细胞中的 miRNA 表达模式来调节 hypoxia-inducible factor-1α (HIF-1α) 活性。

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Camptothecin Chemical Structure

Camptothecin Chemical Structure

CAS No. : 7689-03-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥500
In-stock
100 mg ￥395
In-stock
500 mg ￥1252
In-stock
1 g   询价  
5 g   询价  

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Customer Review

Other Forms of Camptothecin:

Camptothecin 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 38 篇科研文献

WB
IF
IP
Cell Viability Assay

    Camptothecin purchased from MCE. Usage Cited in: Arch Cancer Res. 2023 Jan 30.

    Camptothecin (CPT; 10-5 -10-10 M; 72 h) demonstrates a strong antitumor activity and an inhibitory of proliferation (IC50 = 25.36 nM) in U937 cells.

    Camptothecin purchased from MCE. Usage Cited in: Cell Death Differ. 2020 Nov;27(11):3162-3176.  [Abstract]

    293T cells transfected with Flag-TopBP1 and GFP-tagged RBMX and its domain (RRM, RBM1CTR, and C-RBD) are treated with 100 nM CPT for 24 h and subjected to immunoprecipitation with Flag-beads. Immunoprecipitates are immunoblotted with Flag and GFP antibodies, respectively.

    Camptothecin purchased from MCE. Usage Cited in: Nat Commun. 2019 Aug 21;10(1):3761.  [Abstract]

    Genotoxic stress-induced β-catenin signaling is activated via a TCF- or FOXO-independent mechanism. Representative images of the subcellular localization of β-catenin in the indicated cells treated with CPT (10 μM, 1 h), IR (10 Gy), and CDDP (10 μM, 1 h), as analyzed by immunofluorescence staining.

    Camptothecin purchased from MCE. Usage Cited in: Nat Commun. 2018 Oct 8;9(1):4139.  [Abstract]

    One hour after treated with UV or 2 h after treated with MMS, Camptothecin (CPT) (10 μM), or Doxorubicin (DOX; 0.5 μM), HEK293T cells expressing HA-SUMO2 and His-RhoB are subjected to sumoylation assay to detect the SUMO2 conjugation of RhoB.

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    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Camptothecin (CPT), a kind of alkaloid, is a DNA topoisomerase I (Topo I) inhibitor with an IC50 of 679 nM[1]. Camptothecin (CPT) exhibits powerful antineoplastic activity against colorectal, breast, lung and ovarian cancers, modulates hypoxia-inducible factor-1α (HIF-1α) activity by changing microRNAs (miRNA) expression patterns in human cancer cells[2][3].

    IC50 & Target[3]

    Topoisomerase I

    679 nM (IC50)

    Camptothecins

     

    体外研究
    (In Vitro)

    Camptothecin (0.1 μM 至 5 μM；72 小时) 处理高 TOP1 酶活性 MCF7 (Luminal 亚型) 和 HCC1419 (HER2 亚型) 细胞，细胞表现出高敏感性，IC50 值分别为 0.089 μM 和 0.067 μM[4]。
    Camptothecin (0.5 μM；6 和 24 小时) 降低去铁胺激活的 VEGF 表达。
    Camptothecin (0.5 μM；8 至 24 小时) 强烈阻止去铁胺依赖的 HIF-1a 积累[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Camptothecin 相关抗体:

    Cell Viability Assay[4]

    Cell Line: MCF7 (Luminal subtype) and HCC1419 (HER2 subtype)
    Concentration: 0.1 μM to 5 μM
    Incubation Time: 72 hours
    Result: High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward CPT (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC50 values of 0.089 μM and 0.067 μM, respectively.

    RT-PCR[2]

    Cell Line: HeLa and HEK293 cell lines
    Concentration: 0.5 μmol/L
    Incubation Time: 6 and 24 hours
    Result: Reduces desferrioxamine-activated VEGF expression in both cell lines after 6 and 24 hours of treatment, whereas in normoxic condition camptothecin does not affect the VEGF mRNA level.

    Western Blot Analysis[2]

    Cell Line: HeLa and HEK293 cell lines
    Concentration: 0.5 μmol/L
    Incubation Time: 8 to 24 hours
    Result: Strongly prevents the desferrioxamine-dependent HIF-1a accumulation after 8 to 24 hours, whereas it does not affect HIF-1b levels.
    体内研究
    (In Vivo)

    Camptothecin (每隔一天 2 mg/kg) 处理已发展出大量肺转移小鼠，用核因子-kappaB-1 (KINK-1) 激酶抑制剂和 Camptothecin 处理后，在统计学上显著降低了肺转移灶数量[5]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL6 mice (injected with B16F10 melanoma cells)[5]
    Dosage: 2 mg/kg
    Administration: every other day, after 19 days
    Result: Has developed numerous pulmonary metastases.
    Clinical Trial
    分子量

    348.35

    Formula

    C20H16N2O4
    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    中文名称

    喜树碱
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, protect from light

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    溶解性数据
    细胞实验： 

    1M NaOH 中的溶解度 : 10 mg/mL (28.71 mM; ultrasonic and adjust pH to 11 with NaOH)

    DMSO 中的溶解度 : 6.25 mg/mL (17.94 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.8707 mL 14.3534 mL 28.7068 mL
    5 mM 0.5741 mL 2.8707 mL 5.7414 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： 15% Cremophor EL    85% Saline

      Solubility: 10 mg/mL (28.71 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.86%

    COA (297 KB) HNMR (251 KB) RP-HPLC (242 KB) LCMS (94 KB)

    产品使用指南 (1538 KB)
    参考文献

    Camptothecin 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / 1M NaOH 1 mM 2.8707 mL 14.3534 mL 28.7068 mL 71.7669 mL
    5 mM 0.5741 mL 2.8707 mL 5.7414 mL 14.3534 mL
    10 mM 0.2871 mL 1.4353 mL 2.8707 mL 7.1767 mL
    15 mM 0.1914 mL 0.9569 mL 1.9138 mL 4.7845 mL
    1M NaOH 20 mM 0.1435 mL 0.7177 mL 1.4353 mL 3.5883 mL
    25 mM 0.1148 mL 0.5741 mL 1.1483 mL 2.8707 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Camptothecin7689-03-4Campathecin (S)-(+)-Camptothecin CPT喜树碱TopoisomeraseADC CytotoxinMicroRNAInfluenza VirusApoptosisFungalAntibioticADC PayloadmiRNAalkaloidDNA topoisomerase ITop1hypoxia-inducible factor-1αHIF-1αmicroRNAsmiRNInhibitorinhibitorinhibit

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