2. Anti-infection Apoptosis Stem Cell/Wnt JAK/STAT Signaling Cell Cycle/DNA Damage MAPK/ERK Pathway Protein Tyrosine Kinase/RTK NF-κB
  3. Parasite Apoptosis STAT Topoisomerase ERK FAK p38 MAPK NF-κB
  4. Nitidine chloride

Nitidine chloride  (Synonyms: 氯化两面针碱)

目录号: HY-N0498 纯度: 99.75%
COA 产品使用指南 技术支持

Nitidine chloride 是从 Zanthoxylum nitidum (Roxb) DC 中分离得到一种具有抗疟疾活性的化合物。Nitidine chloride 通过多个靶点通路，发挥抗癌活性，如诱导凋亡，抑制 STAT3，DNA 拓扑异构酶 1 和 2A，ERK 和 c-Src/FAK 相关信号通路，具有抗炎活性。Nitidine chloride 通过 MAPK 和 NF-kB 途径抑制 LPS (HY-D1056) 诱导的炎性细胞因子的产生。

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Nitidine chloride Chemical Structure

Nitidine chloride Chemical Structure

CAS No. : 13063-04-2

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规格 价格 是否有货 数量
1 mg ￥304
In-stock
5 mg ￥800
In-stock
10 mg ￥1400
In-stock
50 mg   询价  
100 mg   询价  

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Customer Review

Other Forms of Nitidine chloride:

Nitidine chloride 相关抗体

Top Publications Citing Use of Products

查看 Parasite 亚型特异性产品:

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

查看 STAT 亚型特异性产品:

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STAT3 STAT5 STAT6 STAT1

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Topoisomerase Topo I Topo II

查看 ERK 亚型特异性产品:

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ERK ERK1 ERK2 ERK5 ERK8

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Nitidine chloride, a potential anti-malarial lead compound derived from Zanthoxylum nitidum (Roxb) DC, exerts potent anticancer activity through diverse pathways, including inducing apoptosis, inhibiting STAT3 signaling cascade, DNA topoisomerase 1 and 2A, ERK and c-Src/FAK associated signaling pathway, also has anti-inflammatory activity. Nitidine chloride inhibits LPS-induced inflammatory cytokines production via MAPK and NF-kB pathway[1][2][3][4][5].

IC50 & Target

Plasmodium

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
HCT-116 IC50
4.42 μM
Compound: 1c
Antiproliferative activity against human HCT116 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Antiproliferative activity against human HCT116 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
[PMID: 26026362]
HeLa IC50
0.05 μg/mL
Compound: 1
Growth inhibition of human HeLa S3 cells after 72 hrs
Growth inhibition of human HeLa S3 cells after 72 hrs
[PMID: 10395504]
HL-60 IC50
3.41 μM
Compound: 1c
Antiproliferative activity against human HL60 cells assessed as inhibition of cell viability after 48 hrs by WST-1 assay
Antiproliferative activity against human HL60 cells assessed as inhibition of cell viability after 48 hrs by WST-1 assay
[PMID: 26026362]
HT-29 IC50
2.75 μM
Compound: 1, 1-k6932
Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay
Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay
[PMID: 21435753]
体外研究
(In Vitro)

Nitidine chloride (0-32 μM，24-48 h) 抑制 A498 和 768-O 细胞增殖[1]。
Nitidine chloride (8-16 μM，24 h)诱导 A498 和 768-O 细胞凋亡[1]。
Nitidine chloride (0-16 μM，48 h) 诱导 A498 细胞凋亡，诱导细胞周期阻滞，抑制 ERK 通路[1]。
Nitidine chloride (0-10 μM，24 h) 诱导 HSC3 和 HSC4 细胞凋亡，在 OSCC 细胞中通过 STAT3 去磷酸化诱导凋亡[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Nitidine chloride 相关抗体:

Cell Viability Assay[1]

Cell Line: 768-O, A498
Concentration: 0, 4, 6, 8, 16, 32 μM
Incubation Time: 24, 48 h
Result: Inhibited cell proliferation in a time- and dose-dependent manner.

Apoptosis Analysis[1]

Cell Line: 768-O, A498
Concentration: 8, 16 μM
Incubation Time: 24 h
Result: Increased apoptosis rate in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: A498
Concentration: 0, 8, 16 μM
Incubation Time: 48 h
Result: Increased Bax expression, decreased Bcl-2 expression, increased p53 expression, decreased pro-caspase-3 expression, increased cleaved caspase-3 expression and PARP cleavage level, inhibited ERK1/2, p38 and Akt phosphorylation.

Cell Viability Assay[2]

Cell Line: HSC3, HSC4
Concentration: 0, 0.1, 5, 10 μM
Incubation Time: 24 h
Result: Reduced cell viability.

Western Blot Analysis[2]

Cell Line: HSC3, HSC4, OSCC
Concentration: 0, 0.1, 5, 10 μM
Incubation Time: 24 h
Result: Dephosphorylated STAT3 and induced the cleavage of PARP and caspase-3 in a concentration- and time-dependent manner.
体内研究
(In Vivo)

Nitidine chloride (5 mg/kg，腹腔注射，每日一次，5 周) 抑制小鼠肿瘤生长，诱导体内肾癌细胞凋亡[1]。
Nitidine chloride (0.375-10 mg/kg，腹腔注射，每日一次，3 周) 通过 AGT4B 介导的自噬下调诱导小鼠心肌肥厚[3]。
Nitidine chloride (5-10 mg/kg，腹腔注射，单次剂量) 在内毒素小鼠中通过 Akt 介导的 IL-10 产生发挥抗炎作用[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A549 nude mouse xenograft model[1]
Dosage: 5 mg/kg; daily; 5 weeks
Administration: Intraperitoneal injection (i.p.)
Result: Showed non-toxic effects, reduced tumor growth, and induced apoptosis in renal cancer cells in vivo.
Animal Model: Mice, beagles[3]
Dosage: 2.5, 5, 10 mg/kg; 0.375, 0.75, 1.5 mg/kg; daily; 4 weeks
Administration: Intraperitoneal injection (i.p.)
Result: Both induced dose-dependent cardiac hypertrophy and dysfunction, while medium and high doses led to death in beagle dogs. Cardiac autophagy levels in cardiomyocytes were reduced. Overexpression of ATG4B reversed cardiac hypertrophy and reduced autophagy levels.
Animal Model: LPS induced endotoxemic mice[4]
Dosage: 5, 10 mg/kg; single dose
Administration: Intraperitoneal injection (i.p.)
Result: Increased IL-10 production, inhibited inflammatory response, reduced mortality, and enhanced Akt activation.
分子量

383.82

Formula

C21H18ClNO4
CAS 号
性状

固体

颜色

Light yellow to yellow

中文名称

氯化两面针碱
结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 2 mg/mL (5.21 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

*Nitidine chloride is usually formulated as a suspension.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6054 mL 13.0269 mL 26.0539 mL
5 mM 0.5211 mL 2.6054 mL 5.2108 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： 0.5% CMC-Na/0.5% Tween-80 in Saline water

    Solubility: 1 mg/mL (2.61 mM); 悬浊液; 超声助溶

  • 方案 二

    请依序添加每种溶剂： 15% Cremophor EL    85% Saline

    Solubility: 2 mg/mL (5.21 mM); 悬浊液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.75%

COA (289 KB) RP-HPLC (244 KB) MS (68 KB)

产品使用指南 (1538 KB)
参考文献

Nitidine chloride 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6054 mL 13.0269 mL 26.0539 mL 65.1347 mL
5 mM 0.5211 mL 2.6054 mL 5.2108 mL 13.0269 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitidine13063-04-2氯化两面针碱ParasiteApoptosisSTATTopoisomeraseERKFAKp38 MAPKNF-κBExtracellular signal regulated kinasesPTK2 protein tyrosine kinase 2PTK2Focal adhesion kinaseNuclear factor-κBNuclear factor-kappaBAnti-tumoranti-inflammatoryapoptosisAktInhibitorinhibitorinhibit

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